FastQCFastQC Report
Wed 25 May 2016
SRR1032963_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032963_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3575712
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48350.031.8856411
TCAACGC46050.030.926934
GGTATCA30600.030.566761
ATCAACG48150.029.675713
CAACGCA48500.029.5580565
AACGCAG50350.028.4716136
GTACATG105600.027.684981
TACATGG105100.026.6095872
TATCAAC57600.025.3782542
ACATGGG108250.024.6629093
ACGCAGA61100.022.7696367
CGCAGAG63300.021.6067188
GAGTACA76050.020.3336091
CATGGGG74100.019.47354
AGTACAT75750.018.4288252
ACACCGT5650.018.3014686
GTATAGA16600.017.5550211
GCAGAGT80250.016.984499
TCTATAC14150.016.6087673
AGAGTAC62700.015.96634510-11
Produced by FastQC (version 0.11.5)