##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1032963_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3575712
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.778133417903902	33.0	31.0	34.0	30.0	34.0
2	31.829147313877627	34.0	31.0	34.0	30.0	34.0
3	31.976002541591715	34.0	31.0	34.0	30.0	34.0
4	35.31887691178708	37.0	35.0	37.0	33.0	37.0
5	35.36937454694338	37.0	35.0	37.0	33.0	37.0
6	35.42937126927448	37.0	37.0	37.0	35.0	37.0
7	35.392436806991164	37.0	37.0	37.0	35.0	37.0
8	35.37784474812289	37.0	37.0	37.0	35.0	37.0
9	37.053649175325084	39.0	38.0	39.0	35.0	39.0
10-11	37.049606903464266	39.0	38.0	39.0	35.0	39.0
12-13	36.995600316804044	39.0	38.0	39.0	35.0	39.0
14-15	38.496328982871105	41.0	39.0	41.0	35.0	41.0
16-17	38.52163275453951	41.0	39.0	41.0	35.0	41.0
18-19	38.53453256302521	41.0	39.0	41.0	35.0	41.0
20-21	38.5784119358606	41.0	39.0	41.0	35.0	41.0
22-23	38.56823885704442	41.0	39.0	41.0	35.0	41.0
24-25	38.59472644888626	41.0	39.0	41.0	34.5	41.0
26-27	38.56669622721293	41.0	39.0	41.0	34.0	41.0
28-29	38.54829122144065	41.0	39.0	41.0	34.0	41.0
30-31	38.50486728237621	41.0	39.0	41.0	34.0	41.0
32-33	38.456472305375826	41.0	39.0	41.0	34.0	41.0
34-35	38.39176966713203	41.0	39.0	41.0	34.0	41.0
36-37	38.30841256230927	41.0	39.0	41.0	34.0	41.0
38-39	38.21871797840542	41.0	39.0	41.0	33.5	41.0
40-41	38.14153698620024	41.0	38.5	41.0	33.0	41.0
42-43	38.03734878536079	40.5	38.0	41.0	33.0	41.0
44-45	37.91211540526754	40.0	38.0	41.0	33.0	41.0
46-47	37.79826758978352	40.0	38.0	41.0	33.0	41.0
48-49	37.6740352690597	40.0	38.0	41.0	33.0	41.0
50-51	37.542598928549054	40.0	38.0	41.0	32.0	41.0
52-53	37.391403166697984	40.0	37.0	41.0	32.0	41.0
54-55	37.23452420105423	40.0	37.0	41.0	32.0	41.0
56-57	37.0556294802266	40.0	36.5	41.0	31.0	41.0
58-59	36.85048893758781	40.0	36.0	41.0	31.0	41.0
60-61	36.611547434468996	39.0	35.5	41.0	31.0	41.0
62-63	36.36016113713856	39.0	35.0	41.0	30.5	41.0
64-65	36.08381449624578	39.0	35.0	41.0	30.0	41.0
66-67	35.78857735186726	38.0	35.0	40.0	30.0	41.0
68-69	35.47845352198387	37.5	35.0	40.0	30.0	41.0
70-71	35.13887849468861	37.0	35.0	40.0	29.0	41.0
72-73	34.84247319135322	36.5	34.5	39.0	29.0	41.0
74-75	34.560914581487545	36.0	34.0	39.0	29.0	41.0
76-77	34.22936522851953	35.5	34.0	38.5	29.0	40.0
78-79	33.85402879202799	35.0	34.0	37.0	29.0	39.5
80-81	33.526838431059325	35.0	34.0	37.0	29.0	39.0
82-83	33.20066045587564	35.0	34.0	36.5	28.5	39.0
84-85	33.024732137263854	35.0	34.0	36.0	29.0	37.5
86-87	32.78705625620856	35.0	34.0	36.0	28.5	37.0
88-89	32.548511177634	35.0	34.0	35.5	28.0	37.0
90-91	32.34285633182986	35.0	34.0	35.0	27.0	36.0
92-93	32.15256765645555	35.0	34.0	35.0	27.0	36.0
94-95	31.971189374312026	35.0	34.0	35.0	27.0	36.0
96-97	31.80302300632713	35.0	33.5	35.0	26.5	36.0
98-99	31.625505074234166	35.0	33.0	35.0	25.5	35.5
100	31.49875577227696	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.14212961497715781
1101	2	0.25160458087641047
1101	3	0.22875879193718518
1101	4	0.1545466612017563
1101	5	0.14056883401912756
1101	6	0.1575323635974044
1101	7	0.1783238005026604
1101	8	0.16126420100894023
1101	9	0.15365102176562573
1101	10-11	0.16160779509975498
1101	12-13	-0.09519619281054048
1101	14-15	-0.016427683259713888
1101	16-17	-0.018330525344119053
1101	18-19	0.05451228556623988
1101	20-21	0.009640655251665464
1101	22-23	-0.0437731245859041
1101	24-25	0.04453849951158162
1101	26-27	0.06892066053237755
1101	28-29	0.03601376100101561
1101	30-31	0.0516895283861416
1101	32-33	0.05099517336398662
1101	34-35	-0.008987842679445635
1101	36-37	0.010086360006454242
1101	38-39	0.015191190795796672
1101	40-41	-0.00818024398745365
1101	42-43	-0.041671427580389775
1101	44-45	-0.04700594494377697
1101	46-47	-0.0810873929381799
1101	48-49	-0.07030488435109561
1101	50-51	-0.025718203996270006
1101	52-53	-0.10950590560045725
1101	54-55	-0.032116082378074395
1101	56-57	-0.06698487412814558
1101	58-59	-0.04620132694365964
1101	60-61	-0.052057823749613874
1101	62-63	-0.03752540158070161
1101	64-65	-0.09251566706235792
1101	66-67	-0.05310035873670671
1101	68-69	0.006300732001051301
1101	70-71	-0.034383199483684734
1101	72-73	-0.060558004608459726
1101	74-75	-0.0674366986827124
1101	76-77	-0.041341054292544754
1101	78-79	0.015601061050169562
1101	80-81	0.01953885792173793
1101	82-83	-0.030216926710231462
1101	84-85	-0.013180006498075159
1101	86-87	-0.016185032446912828
1101	88-89	-0.04861816733866675
1101	90-91	-0.06710840339764701
1101	92-93	-0.046893048745971555
1101	94-95	-0.04629250059288026
1101	96-97	-0.07486585948545965
1101	98-99	-0.022036674271838308
1101	100	-0.033537748498684294
1102	1	0.3027731399192426
1102	2	0.3342537782653139
1102	3	0.3852543771009671
1102	4	0.34389783549961095
1102	5	0.3056668980801831
1102	6	0.2956114188372396
1102	7	0.3327554682146925
1102	8	0.3285859233547015
1102	9	0.34915061312137396
1102	10-11	0.36227174169512466
1102	12-13	0.4244858882974256
1102	14-15	0.4959227708950493
1102	16-17	0.4181184722631528
1102	18-19	0.3935392265208364
1102	20-21	0.44598743899967275
1102	22-23	0.3811438055348688
1102	24-25	0.4389269164005185
1102	26-27	0.4858753844388062
1102	28-29	0.49258471832673223
1102	30-31	0.4875109244500635
1102	32-33	0.45216343131459524
1102	34-35	0.5215288230368884
1102	36-37	0.535454424933377
1102	38-39	0.5439620691380753
1102	40-41	0.5421312337419621
1102	42-43	0.5429760296386803
1102	44-45	0.535329451673455
1102	46-47	0.5400002132090052
1102	48-49	0.512942770960116
1102	50-51	0.4994443350466824
1102	52-53	0.49708143973244034
1102	54-55	0.49790807726138553
1102	56-57	0.4580046827409845
1102	58-59	0.4819452021144386
1102	60-61	0.5358318824277788
1102	62-63	0.5388117098640777
1102	64-65	0.563701385334916
1102	66-67	0.564830972134196
1102	68-69	0.560361578813307
1102	70-71	0.49138166217914403
1102	72-73	0.4825952718314639
1102	74-75	0.5294274688128553
1102	76-77	0.4552550974823646
1102	78-79	0.4305014975709369
1102	80-81	0.4422728041158521
1102	82-83	0.4513189632367016
1102	84-85	0.4206074604777683
1102	86-87	0.47917663355451623
1102	88-89	0.42801196420887777
1102	90-91	0.39230523961833086
1102	92-93	0.4209965518385914
1102	94-95	0.41992421390733625
1102	96-97	0.4475361450888222
1102	98-99	0.4890006063326595
1102	100	0.5215219008908818
1103	1	0.20028452276288888
1103	2	0.20531507174454333
1103	3	0.24389919194274157
1103	4	0.21526159317242133
1103	5	0.2451197128572602
1103	6	0.14613959895709172
1103	7	0.20817046688028995
1103	8	0.20909606884483622
1103	9	0.285902924061034
1103	10-11	0.2602214550011226
1103	12-13	0.2571932663210603
1103	14-15	0.3458516028495069
1103	16-17	0.3702504000275866
1103	18-19	0.33169937079787104
1103	20-21	0.3092327018766383
1103	22-23	0.28950447386751677
1103	24-25	0.30688301865605894
1103	26-27	0.3408971796527567
1103	28-29	0.37561069466335084
1103	30-31	0.3562077404887489
1103	32-33	0.33805925908792744
1103	34-35	0.36054160902325094
1103	36-37	0.29617609317706695
1103	38-39	0.31001935941474557
1103	40-41	0.35847404536499283
1103	42-43	0.3821137640686487
1103	44-45	0.3828377209151057
1103	46-47	0.3531093267624783
1103	48-49	0.3781265139140757
1103	50-51	0.38800633616272506
1103	52-53	0.35492116605895774
1103	54-55	0.4167453693172476
1103	56-57	0.388958011060204
1103	58-59	0.40647913228887234
1103	60-61	0.41082027718399416
1103	62-63	0.4452974236876628
1103	64-65	0.406708801368751
1103	66-67	0.3869869191140083
1103	68-69	0.4183939440994777
1103	70-71	0.4013829965799971
1103	72-73	0.42787125018990224
1103	74-75	0.4027888650005167
1103	76-77	0.40186159480143147
1103	78-79	0.3449594882058662
1103	80-81	0.34093613882402707
1103	82-83	0.3895025201832709
1103	84-85	0.3676501855669301
1103	86-87	0.2747513963950894
1103	88-89	0.33751706323759834
1103	90-91	0.3001486052199098
1103	92-93	0.3343339692472682
1103	94-95	0.3116244832136914
1103	96-97	0.31702616134424844
1103	98-99	0.3774981114074265
1103	100	0.3663691726476834
1104	1	0.1266318359992944
1104	2	0.17186960921707595
1104	3	0.23987368002892495
1104	4	0.2282833901891408
1104	5	0.21981232743215884
1104	6	0.21443897735302642
1104	7	0.2886686924287716
1104	8	0.3074534765168764
1104	9	0.3417234183364215
1104	10-11	0.37619771223450016
1104	12-13	0.36285004427654854
1104	14-15	0.4123274647549309
1104	16-17	0.42002849218174987
1104	18-19	0.3338275737499501
1104	20-21	0.2926644979760269
1104	22-23	0.34277382356395236
1104	24-25	0.3449690603655071
1104	26-27	0.32480324448903275
1104	28-29	0.3194544680452722
1104	30-31	0.27229985034187365
1104	32-33	0.28255530799252426
1104	34-35	0.3306033170022573
1104	36-37	0.32909751995215686
1104	38-39	0.31168312222545325
1104	40-41	0.38277587186126283
1104	42-43	0.3437378567956699
1104	44-45	0.2562982196336421
1104	46-47	0.29211969327860743
1104	48-49	0.32456105827598947
1104	50-51	0.3037351780047075
1104	52-53	0.27433432714121864
1104	54-55	0.33579237621349733
1104	56-57	0.37289418572090227
1104	58-59	0.34068666979198525
1104	60-61	0.34761577749694794
1104	62-63	0.28832913666521875
1104	64-65	0.2818341549641801
1104	66-67	0.3277269624347099
1104	68-69	0.28785490647810263
1104	70-71	0.2583795837172076
1104	72-73	0.2716722457798326
1104	74-75	0.2978289032665131
1104	76-77	0.3053846470702126
1104	78-79	0.23401079160243654
1104	80-81	0.24904111072048352
1104	82-83	0.1969264016110941
1104	84-85	0.20151088285429353
1104	86-87	0.16960944963425106
1104	88-89	0.18921837752363047
1104	90-91	0.18133804625051653
1104	92-93	0.19180435709300525
1104	94-95	0.1907783909072691
1104	96-97	0.2425263432512743
1104	98-99	0.18336462847143054
1104	100	0.1872423949979698
1105	1	0.1347766158663788
1105	2	0.17094116988228336
1105	3	0.21077036684230777
1105	4	0.22671727465708358
1105	5	0.23160284683303445
1105	6	0.24172531762719274
1105	7	0.22732976081715606
1105	8	0.2456210634884357
1105	9	0.34468371344320303
1105	10-11	0.3019684422368982
1105	12-13	0.30965678262325014
1105	14-15	0.34933181374071154
1105	16-17	0.339614052350278
1105	18-19	0.3368479129281141
1105	20-21	0.36393440004521693
1105	22-23	0.38206504687864395
1105	24-25	0.34610670822819856
1105	26-27	0.3959291001263239
1105	28-29	0.3867842699910682
1105	30-31	0.372657748610969
1105	32-33	0.4257090896489899
1105	34-35	0.4271253670841304
1105	36-37	0.4387450779423361
1105	38-39	0.40474538766787305
1105	40-41	0.38733158133667445
1105	42-43	0.3953657817467402
1105	44-45	0.4190784759858559
1105	46-47	0.4020850509276457
1105	48-49	0.4015503157648439
1105	50-51	0.35796517342667755
1105	52-53	0.352240452778787
1105	54-55	0.3168798659514849
1105	56-57	0.3249012079473701
1105	58-59	0.30833975287517745
1105	60-61	0.22965677005548457
1105	62-63	0.2440714638562298
1105	64-65	0.30719774981719183
1105	66-67	0.2829310440370918
1105	68-69	0.27168639915221604
1105	70-71	0.29714235244687615
1105	72-73	0.2806581618661781
1105	74-75	0.2579834127209182
1105	76-77	0.24001868309102292
1105	78-79	0.28131150091614643
1105	80-81	0.30810378234119895
1105	82-83	0.2847468084955267
1105	84-85	0.26701573815364554
1105	86-87	0.22720803243098686
1105	88-89	0.2916139913320279
1105	90-91	0.2703732051510883
1105	92-93	0.2663700815783159
1105	94-95	0.2917572761629401
1105	96-97	0.30941580964454474
1105	98-99	0.29699697658255175
1105	100	0.3067229021482234
1106	1	-0.1055842560297755
1106	2	-0.06491517378321987
1106	3	-0.02964993905989033
1106	4	-0.011998449716045911
1106	5	-0.032494899400987265
1106	6	-0.04453590329961088
1106	7	-0.02592316554247276
1106	8	-0.08152505932172005
1106	9	-0.001871349209487505
1106	10-11	-0.054213114344705104
1106	12-13	-0.04246635666528675
1106	14-15	0.03543079050778175
1106	16-17	-0.048908038166494805
1106	18-19	-0.01841430278870604
1106	20-21	0.03506497577354395
1106	22-23	0.013020085159844541
1106	24-25	0.006871281517661032
1106	26-27	0.048548865698677446
1106	28-29	-0.005879089534985837
1106	30-31	0.0272604390425073
1106	32-33	-0.001454535942109203
1106	34-35	0.026125160381916146
1106	36-37	0.09338364231285823
1106	38-39	0.05666671469612794
1106	40-41	0.06376295861975478
1106	42-43	0.04389951278729143
1106	44-45	-0.010367344258945366
1106	46-47	0.04528730250132895
1106	48-49	0.0028019461072545937
1106	50-51	-0.003158053838426156
1106	52-53	-0.012782570602766441
1106	54-55	-0.0065006321211456
1106	56-57	-0.03877572417031416
1106	58-59	-0.047758453741053586
1106	60-61	-0.03682460012398536
1106	62-63	-0.07885338536645747
1106	64-65	-0.07665709853142033
1106	66-67	-0.08154856746523365
1106	68-69	-0.035527906919114116
1106	70-71	-0.028825268760769518
1106	72-73	-0.04983327693216921
1106	74-75	-0.08056168978886546
1106	76-77	-0.03796491113030953
1106	78-79	-0.04830575974418139
1106	80-81	0.048494406829604486
1106	82-83	0.0018729700210684541
1106	84-85	0.04794206304796944
1106	86-87	-0.009042355135676416
1106	88-89	-2.455926612441317E-4
1106	90-91	0.06892555647424814
1106	92-93	4.6724984332513486E-4
1106	94-95	0.016538751202503477
1106	96-97	0.010692238559052214
1106	98-99	-0.001960878372280206
1106	100	0.03762330385092483
1107	1	0.056678708949004175
1107	2	0.04663239423178567
1107	3	0.07275049745817697
1107	4	0.08532684500590193
1107	5	0.05597903508965629
1107	6	0.08476310125479358
1107	7	0.03331971231159514
1107	8	0.0855992953450766
1107	9	0.039091233678369974
1107	10-11	-0.02023882858913595
1107	12-13	-0.012532873376351006
1107	14-15	0.06534421625946152
1107	16-17	0.050094348783652265
1107	18-19	0.03376689418180945
1107	20-21	0.057867230474677456
1107	22-23	0.08673318600005331
1107	24-25	0.08467993880487512
1107	26-27	0.1686465180379031
1107	28-29	0.1445943268312746
1107	30-31	0.13067522616082528
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2216	36-37	-0.3531200329638864
2216	38-39	-0.30928598704451815
2216	40-41	-0.35335728576462344
2216	42-43	-0.3295713402224578
2216	44-45	-0.3215146097078545
2216	46-47	-0.24106643521655968
2216	48-49	-0.24494718959349626
2216	50-51	-0.2846332352385659
2216	52-53	-0.2565432952015456
2216	54-55	-0.26573626685699736
2216	56-57	-0.3260840602190953
2216	58-59	-0.2769247616313777
2216	60-61	-0.23453248357992607
2216	62-63	-0.2186687379549639
2216	64-65	-0.2741997478935332
2216	66-67	-0.30557033210457263
2216	68-69	-0.2778222914924555
2216	70-71	-0.3238177358644947
2216	72-73	-0.3675292236880736
2216	74-75	-0.3850589301656413
2216	76-77	-0.3145061720983193
2216	78-79	-0.365070702989982
2216	80-81	-0.305352660462205
2216	82-83	-0.3783805146117629
2216	84-85	-0.37670288906734584
2216	86-87	-0.3603329088218459
2216	88-89	-0.35848893644552504
2216	90-91	-0.39967027170255065
2216	92-93	-0.4609785034547471
2216	94-95	-0.34767138086658633
2216	96-97	-0.33738142793258774
2216	98-99	-0.33486878737871706
2216	100	-0.38188188869800754
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	42035.0
3	6347.0
4	4165.0
5	3582.0
6	2901.0
7	3394.0
8	4075.0
9	4354.0
10	4660.0
11	5105.0
12	5610.0
13	6191.0
14	6614.0
15	6949.0
16	6679.0
17	6731.0
18	6466.0
19	6317.0
20	6646.0
21	6744.0
22	8003.0
23	11396.0
24	17177.0
25	23268.0
26	24504.0
27	21685.0
28	23404.0
29	29015.0
30	35882.0
31	43484.0
32	55900.0
33	75530.0
34	109113.0
35	169966.0
36	320361.0
37	702365.0
38	1200826.0
39	557064.0
40	1204.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.6560169052717	19.389143121037826	12.999678128105014	23.955161845585458
2	15.399266841526762	19.775378201900434	40.695529311924304	24.129825644648495
3	18.448235952373555	25.067840449897993	29.56536488575514	26.91855871197331
4	12.170810476740158	16.220619049565055	38.986233701819216	32.622336771875574
5	12.819228871296895	38.21845651663401	35.728806500161916	13.233508111907177
6	31.42327892455807	37.84865709708154	17.6557949880035	13.072268990356894
7	27.88517275671536	32.16286878897743	22.277741375599295	17.674217078707915
8	25.226087107422934	37.05897269824992	19.949685647254288	17.76525454707286
9	24.959596949128823	17.033125743474915	20.79683717024701	37.210440137149256
10-11	23.976357263661853	27.015275973037706	28.76470411242825	20.24366265087219
12-13	24.472641096460933	24.242821604543657	28.9189387383131	22.365598560682308
14-15	21.679865463654014	26.383949456787253	25.73567392093205	26.20051115862669
16-17	21.61278562967099	29.783167397511978	26.348691230625953	22.255355742191078
18-19	21.51950299725324	28.83324591694566	28.176923305134316	21.47032778066678
20-21	22.560657160049818	28.154969982787332	27.755090331518772	21.529282525644074
22-23	22.28038725062135	27.80797283983789	27.837304919280804	22.07433499025996
24-25	22.114154727152123	27.94575509324437	27.773610570773638	22.166479608829874
26-27	21.854292865753326	27.95034723961787	27.846467834329214	22.348892060299594
28-29	21.90777934413814	28.029263044707882	27.90688822596115	22.15606938519283
30-31	22.071727632486006	28.415894430467116	27.56500484627254	21.947373090774335
32-33	21.79485187998029	28.119454341876406	27.995801165804828	22.089892612338478
34-35	21.940867452749657	27.894424557583424	28.059748278052837	22.104959711614082
36-37	22.1495001069795	28.228496455962997	27.602725857231835	22.019277579825665
38-39	22.066826035490372	28.407942954114734	27.69418370030004	21.831047310094853
40-41	21.859775373479586	28.06181609441826	28.02730682435509	22.051101707747065
42-43	21.76290805601299	28.03080291711339	27.99333907769541	22.21294994917821
44-45	21.855199934392218	28.132470617146975	27.947458916066548	22.064870532394263
46-47	21.906945967597018	28.268498816279532	27.817710966194692	22.006844249928765
48-49	21.855431800760332	28.13280479907539	27.857075177854075	22.15468822231021
50-51	21.86239673863145	28.05635736215209	27.879995678082054	22.2012502211344
52-53	21.885054273130812	28.002349064488985	27.938485973073092	22.17411068930711
54-55	21.98398422607501	28.4430442958641	27.630261124071303	21.942710353989582
56-57	21.804151392257737	28.168382312909745	27.80822046260658	22.219245832225933
58-59	21.715608963409334	27.832093313775168	28.109217076735472	22.343080646080026
60-61	21.994842495419377	28.23511234492638	27.740116742212663	22.02992841744158
62-63	21.810268155478536	28.193685495140507	27.784339205029323	22.211707144351635
64-65	21.793868451326322	27.918839411420592	27.929097845588874	22.35819429166422
66-67	21.675368812561544	28.03171398965819	27.80260039936384	22.490316798416426
68-69	22.00779830489522	28.085191603715902	27.78963078198992	22.117379309398956
70-71	21.907441168472992	27.884057881772538	27.86478709559863	22.343713854155837
72-73	21.726091596690637	27.979934902069008	27.953428865158163	22.34054463608219
74-75	21.7754216948403	28.049952627991885	27.872877880980802	22.30174779618701
76-77	21.80047092081463	27.990217722156718	27.76824818156691	22.441063175461736
78-79	21.877991368066326	28.055253891927297	27.84309417316487	22.22366056684151
80-81	21.793452933248805	27.840022167828938	27.945431314017654	22.421093584904604
82-83	21.657074178765388	27.89845164566004	28.06897802567818	22.375496149896396
84-85	21.714352568819486	27.87109734160064	27.88985019826248	22.524699891317397
86-87	21.86351682895737	27.963724357852083	27.797815999443564	22.37494281374698
88-89	21.56708779485807	27.85593794108534	27.90232930845655	22.67464495560003
90-91	21.587397235123127	27.798672362034637	27.96343761842415	22.650492784418084
92-93	21.816776824773672	27.908178922831855	27.80816410323007	22.4668801491644
94-95	21.55542917405084	27.63742402649235	28.065528934585256	22.741617864871554
96-97	21.607400770141556	27.58950897983042	28.082682814161124	22.720407435866903
98-99	21.625192694998383	27.722359112948098	27.945868518479127	22.706579673574392
100	21.72077065188704	27.54479605107938	27.88557153360442	22.84886176342916
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3100.0
1	1795.5
2	481.0
3	406.5
4	310.5
5	276.5
6	247.0
7	231.0
8	235.5
9	231.5
10	234.0
11	250.0
12	266.0
13	284.0
14	330.0
15	414.0
16	516.5
17	684.5
18	971.5
19	1386.5
20	2014.5
21	2951.0
22	4406.0
23	6723.0
24	9960.5
25	14021.5
26	19398.0
27	26227.5
28	34668.5
29	44681.0
30	54940.5
31	65344.5
32	75732.0
33	86149.0
34	96111.5
35	103709.0
36	110287.5
37	117707.0
38	124033.0
39	126753.5
40	128160.5
41	130385.0
42	131633.5
43	131296.5
44	129732.0
45	128060.0
46	126561.5
47	123559.5
48	119827.0
49	116627.5
50	113599.0
51	109684.5
52	102662.0
53	97178.5
54	93535.5
55	88001.5
56	81339.5
57	73970.5
58	66788.5
59	60608.0
60	53967.0
61	46192.0
62	39717.5
63	33492.5
64	27107.0
65	21663.0
66	16897.0
67	12618.0
68	9222.5
69	6815.0
70	4882.5
71	3578.5
72	2819.0
73	2011.0
74	1445.5
75	1022.5
76	644.5
77	427.5
78	301.0
79	181.0
80	118.5
81	103.0
82	83.0
83	59.5
84	44.5
85	36.5
86	23.5
87	14.5
88	13.5
89	15.0
90	20.0
91	15.0
92	7.5
93	6.0
94	2.5
95	2.5
96	3.0
97	1.5
98	2.5
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08004000322173598
2	0.08252901799697515
3	0.08364767632292534
4	0.08596889234927198
5	0.08392734090441288
6	0.08401124027885915
7	0.08308834715995024
8	0.0831722465343965
9	0.08283664903661145
10-11	0.08234723601900824
12-13	0.0813264602965787
14-15	0.07966245603672778
16-17	0.07815226729669504
18-19	0.07757895490464557
20-21	0.0772433574068605
22-23	0.07920100947727333
24-25	0.07948067405876089
26-27	0.07883744552133953
28-29	0.07475434263162134
30-31	0.08006796967988473
32-33	0.07873956291781888
34-35	0.07612469908091032
36-37	0.07264287504139036
38-39	0.0743488289884644
40-41	0.07715945803241425
42-43	0.08203960497937195
44-45	0.08292054841105771
46-47	0.086080758181867
48-49	0.0892269847236019
50-51	0.0901638610715852
52-53	0.0902197939878827
54-55	0.09044352565307273
56-57	0.08813629285580046
58-59	0.08726933265318908
60-61	0.08540956318629689
62-63	0.0857032109968588
64-65	0.08507396568851182
66-67	0.08473836819072676
68-69	0.08424895517312357
70-71	0.08240316893530575
72-73	0.0822493534154876
74-75	0.08185782300140504
76-77	0.08178790685603314
78-79	0.08199765529214881
80-81	0.08297648132735522
82-83	0.08252901799697515
84-85	0.08174595716881002
86-87	0.0826828335167933
88-89	0.07967643926580217
90-91	0.07690775990907545
92-93	0.07667004501481103
94-95	0.07707555865796799
96-97	0.07641834689147224
98-99	0.07573316866682775
100	0.0750899401294064
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3575712.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.30539632355509
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.00897238231086	45.98454779720165
2	14.733881547110128	18.065328952598026
3	4.719583169680703	8.680077500977674
4	2.051838348312887	5.031550525407608
5	1.0433421565330703	3.198125220366625
6	0.6276299837850887	2.3086262940294793
7	0.3948528522684497	1.6944627418462417
8	0.27073953739104645	1.327823571217125
9	0.18561813437004143	1.0241453963154863
>10	0.9081379951941958	9.63748719849158
>50	0.042674396062112735	1.773976804812872
>100	0.012592360258679207	1.1600702705707602
>500	9.14244819065937E-5	0.04416593508402471
>1k	4.571224095103733E-5	0.06961179108079511
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.796645814875471E-5	0.0
5	0.0	0.0	0.0	2.796645814875471E-5	0.0
6	0.0	0.0	0.0	2.796645814875471E-5	0.0
7	0.0	0.0	0.0	5.593291629750942E-5	0.0
8	0.0	0.0	0.0	5.593291629750942E-5	0.0
9	1.1186583259501884E-4	0.0	0.0	8.389937444626413E-5	0.0
10-11	1.258490616693962E-4	0.0	0.0	1.1186583259501884E-4	0.0
12-13	1.3983229074377354E-4	0.0	0.0	1.1186583259501884E-4	0.0
14-15	1.3983229074377354E-4	0.0	0.0	1.1186583259501884E-4	0.0
16-17	1.3983229074377354E-4	0.0	0.0	1.538155198181509E-4	0.0
18-19	1.6779874889252827E-4	0.0	0.0	1.8178197796690562E-4	0.0
20-21	2.0974843611566032E-4	0.0	0.0	2.0974843611566032E-4	0.0
22-23	2.2373166519003767E-4	0.0	0.0	2.936478105619244E-4	0.0
24-25	2.2373166519003767E-4	1.3983229074377354E-5	0.0	3.2161426871067916E-4	0.0
26-27	2.2373166519003767E-4	2.796645814875471E-5	0.0	4.055136431569433E-4	0.0
28-29	2.2373166519003767E-4	2.796645814875471E-5	0.0	0.0011745912422476978	0.0
30-31	2.516981233387924E-4	2.796645814875471E-5	0.0	0.004600482365470149	0.0
32-33	2.516981233387924E-4	2.796645814875471E-5	0.0	0.012403124188972714	0.0
34-35	3.3559749778505653E-4	2.796645814875471E-5	0.0	0.023254109950689543	0.0
36-37	3.3559749778505653E-4	2.796645814875471E-5	0.0	0.04336199335964418	0.0
38-39	3.3559749778505653E-4	2.796645814875471E-5	0.0	0.07892134489578578	0.0
40-41	3.3559749778505653E-4	2.796645814875471E-5	0.0	0.12317826491619012	0.0
42-43	3.3559749778505653E-4	2.796645814875471E-5	0.0	0.1678406985797514	0.0
44-45	3.3559749778505653E-4	2.796645814875471E-5	0.0	0.2197744113619889	0.0
46-47	3.3559749778505653E-4	2.796645814875471E-5	0.0	0.28419514770764537	0.0
48-49	3.3559749778505653E-4	2.796645814875471E-5	0.0	0.3598304337709525	0.0
50-51	3.3559749778505653E-4	2.796645814875471E-5	0.0	0.4399543363671347	0.0
52-53	3.6356395593381124E-4	2.796645814875471E-5	0.0	0.5231825158178287	0.0
54-55	3.9153041408256594E-4	2.796645814875471E-5	0.0	0.6149963979201905	0.0
56-57	4.1949687223132064E-4	2.796645814875471E-5	0.0	0.7236600710571769	0.0
58-59	4.4746333038007534E-4	2.796645814875471E-5	0.0	0.8505299084490026	0.0
60-61	4.4746333038007534E-4	5.593291629750942E-5	0.0	0.9877613185849419	0.0
62-63	4.7542978852883005E-4	5.593291629750942E-5	0.0	1.1288800664035583	0.0
64-65	4.7542978852883005E-4	5.593291629750942E-5	0.0	1.2771162778210328	0.0
66-67	4.7542978852883005E-4	5.593291629750942E-5	0.0	1.4414192194449664	0.0
68-69	4.7542978852883005E-4	5.593291629750942E-5	0.0	1.632206396935771	0.0
70-71	4.7542978852883005E-4	5.593291629750942E-5	0.0	1.8417870343025389	0.0
72-73	5.033962466775847E-4	5.593291629750942E-5	0.0	2.0610999991050734	0.0
74-75	5.313627048263395E-4	5.593291629750942E-5	0.0	2.278860825480352	1.3983229074377354E-5
76-77	5.313627048263395E-4	8.389937444626413E-5	0.0	2.513471442890255	2.796645814875471E-5
78-79	5.313627048263395E-4	1.1186583259501884E-4	0.0	2.783711327981672	2.796645814875471E-5
80-81	5.313627048263395E-4	1.1186583259501884E-4	0.0	3.0814002917460916	2.796645814875471E-5
82-83	5.313627048263395E-4	1.1186583259501884E-4	0.0	3.3854795911975013	2.796645814875471E-5
84-85	5.313627048263395E-4	1.1186583259501884E-4	0.0	3.6861609659838375	2.796645814875471E-5
86-87	5.313627048263395E-4	1.1186583259501884E-4	0.0	4.002713305769592	2.796645814875471E-5
88	5.593291629750942E-4	1.1186583259501884E-4	0.0	4.265863693720299	2.796645814875471E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4835	0.0	31.885641	1
TCAACGC	4605	0.0	30.92693	4
GGTATCA	3060	0.0	30.56676	1
ATCAACG	4815	0.0	29.67571	3
CAACGCA	4850	0.0	29.558056	5
AACGCAG	5035	0.0	28.471613	6
GTACATG	10560	0.0	27.68498	1
TACATGG	10510	0.0	26.609587	2
TATCAAC	5760	0.0	25.378254	2
ACATGGG	10825	0.0	24.662909	3
ACGCAGA	6110	0.0	22.769636	7
CGCAGAG	6330	0.0	21.606718	8
GAGTACA	7605	0.0	20.333609	1
CATGGGG	7410	0.0	19.4735	4
AGTACAT	7575	0.0	18.428825	2
ACACCGT	565	0.0	18.301468	6
GTATAGA	1660	0.0	17.555021	1
GCAGAGT	8025	0.0	16.98449	9
TCTATAC	1415	0.0	16.608767	3
AGAGTAC	6270	0.0	15.966345	10-11
>>END_MODULE