FastQCFastQC Report
Wed 25 May 2016
SRR1032963_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032963_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3575712
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62010.17342000698042795No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50980.1425730036423515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53050.044.5500951
GTATCAA93000.040.0641861
TCAACGC107300.033.8921284
ATCAACG108850.033.366293
CAACGCA110150.033.054685
AACGCAG112650.032.404636
TATCAAC116050.031.457892
GTACATG105400.027.4603541
ACGCAGA132800.027.1682477
CGCAGAG136200.026.3864298
TACATGG106550.026.050232
ACATGGG104050.025.6365783
GCAGAGT150250.023.8263729
CATGGGG72750.021.4049434
GAGTACT98700.020.58639712-13
AGAGTAC134050.020.50219710-11
GAGTACA76200.020.3481541
GTGGTAT33250.020.0660881
CAGAGTA147250.019.3343110-11
TATACTG22250.019.027825
Produced by FastQC (version 0.11.5)