FastQCFastQC Report
Wed 25 May 2016
SRR1032962_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032962_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4449210
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG118250.028.1408391
TACATGG113200.027.4032732
ACATGGG123850.024.173993
GTATCAA99850.020.240691
TCAACGC92950.020.1756924
CAACGCA94450.019.8052865
CATGGGG70250.019.7369754
ATCAACG96150.019.55313
GAGTACA87550.019.5411531
AACGCAG100200.018.9032886
GTACGGA3701.6552804E-1017.7840731
TATCAAC108000.017.6688062
AGTACAT91000.017.0958822
CATGGGA48450.016.8795324
ACGCAGA110400.016.6459167
GGTATCA76650.016.3107681
CGCAGAG111350.016.2506438
GGACCGT5300.015.9623436
TATACCG2658.7861845E-615.9623435
GAACCGT3403.6052552E-715.2059586
Produced by FastQC (version 0.11.5)