##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032962_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4449210 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.724966005200923 33.0 31.0 34.0 30.0 34.0 2 31.84418087705458 34.0 31.0 34.0 30.0 34.0 3 31.923592727697727 34.0 31.0 34.0 30.0 34.0 4 35.28714018893242 37.0 35.0 37.0 33.0 37.0 5 35.35677120207857 37.0 35.0 37.0 33.0 37.0 6 35.48050395463464 37.0 37.0 37.0 35.0 37.0 7 35.441064818248634 37.0 37.0 37.0 35.0 37.0 8 35.45553030762765 37.0 37.0 37.0 35.0 37.0 9 37.14019545042828 39.0 39.0 39.0 35.0 39.0 10-11 37.12941814389521 39.0 38.0 39.0 35.0 39.0 12-13 37.0800000449518 39.0 38.0 39.0 35.0 39.0 14-15 38.58780716127133 41.0 39.0 41.0 35.5 41.0 16-17 38.621694076026984 41.0 39.0 41.0 35.5 41.0 18-19 38.646914171279846 41.0 39.5 41.0 35.0 41.0 20-21 38.69316058356428 41.0 40.0 41.0 35.0 41.0 22-23 38.692383816452804 41.0 39.0 41.0 35.0 41.0 24-25 38.73388713951466 41.0 39.0 41.0 35.0 41.0 26-27 38.7129245416602 41.0 39.0 41.0 35.0 41.0 28-29 38.702110824168784 41.0 39.0 41.0 35.0 41.0 30-31 38.66374985671614 41.0 39.0 41.0 35.0 41.0 32-33 38.613729740785445 41.0 39.0 41.0 35.0 41.0 34-35 38.54985503044361 41.0 39.0 41.0 34.0 41.0 36-37 38.47180375842004 41.0 39.0 41.0 34.0 41.0 38-39 38.392918405739444 41.0 39.0 41.0 34.0 41.0 40-41 38.32292260873279 41.0 39.0 41.0 34.0 41.0 42-43 38.23098167989373 41.0 38.5 41.0 33.5 41.0 44-45 38.12367656280553 40.0 38.0 41.0 33.0 41.0 46-47 38.02072637614318 40.0 38.0 41.0 33.0 41.0 48-49 37.90958484764711 40.0 38.0 41.0 33.0 41.0 50-51 37.796381829583225 40.0 38.0 41.0 33.0 41.0 52-53 37.66662665956429 40.0 38.0 41.0 33.0 41.0 54-55 37.52921687220878 40.0 37.5 41.0 32.0 41.0 56-57 37.368053654468994 40.0 37.0 41.0 32.0 41.0 58-59 37.184439484762464 40.0 37.0 41.0 31.5 41.0 60-61 36.96714191957673 40.0 36.0 41.0 31.0 41.0 62-63 36.73881149687248 39.0 36.0 41.0 31.0 41.0 64-65 36.480431357476945 39.0 35.0 41.0 31.0 41.0 66-67 36.20212723157594 39.0 35.0 40.5 30.5 41.0 68-69 35.90107929272837 38.0 35.0 40.0 30.0 41.0 70-71 35.564090141845405 37.5 35.0 40.0 30.0 41.0 72-73 35.268821431220374 37.0 35.0 39.5 30.0 41.0 74-75 34.986639538255105 36.5 35.0 39.0 30.0 41.0 76-77 34.64175640169828 36.0 34.5 39.0 30.0 40.5 78-79 34.247727574108666 36.0 34.0 38.5 29.0 39.5 80-81 33.8903952611812 35.0 34.0 37.0 29.0 39.0 82-83 33.53543887566557 35.0 34.0 37.0 29.0 39.0 84-85 33.33654165570967 35.0 34.0 36.5 29.0 38.5 86-87 33.07676778574174 35.0 34.0 36.0 29.0 37.0 88-89 32.80959226469418 35.0 34.0 36.0 29.0 37.0 90-91 32.58188768343144 35.0 34.0 35.5 28.0 36.5 92-93 32.36961157149247 35.0 34.0 35.0 28.0 36.0 94-95 32.17789247978855 35.0 34.0 35.0 27.0 36.0 96-97 31.998290932547576 35.0 34.0 35.0 27.0 36.0 98-99 31.820009282546792 35.0 34.0 35.0 27.0 36.0 100 31.690777014346367 35.0 34.0 35.0 26.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31945252827126325 1101 2 0.4010494287284452 1101 3 0.39285326980964896 1101 4 0.33704363257236025 1101 5 0.24421606149073227 1101 6 0.26471856359390245 1101 7 0.3212616689657892 1101 8 0.2524784252399357 1101 9 0.30803804335332075 1101 10-11 0.3192863520006668 1101 12-13 0.13210487326657017 1101 14-15 0.1378412796128643 1101 16-17 0.1798607127122409 1101 18-19 0.22403176294744753 1101 20-21 0.2430258418849789 1101 22-23 0.2212992726087606 1101 24-25 0.255110009953718 1101 26-27 0.27583159233486043 1101 28-29 0.2690405527990123 1101 30-31 0.3238921653837039 1101 32-33 0.30481895886221366 1101 34-35 0.324522617078685 1101 36-37 0.2646158226055846 1101 38-39 0.2935937945084035 1101 40-41 0.25754420827518487 1101 42-43 0.20506664310311606 1101 44-45 0.25343728724760695 1101 46-47 0.1901923101996772 1101 48-49 0.24400171536468918 1101 50-51 0.22150438184085885 1101 52-53 0.17906375462345636 1101 54-55 0.190310817940599 1101 56-57 0.21115666052538984 1101 58-59 0.21199761987736565 1101 60-61 0.25080372781135196 1101 62-63 0.24733254989446607 1101 64-65 0.23425030338805897 1101 66-67 0.2718302727391233 1101 68-69 0.23409976714437875 1101 70-71 0.20075624178083018 1101 72-73 0.12207651037234513 1101 74-75 0.1843865777210425 1101 76-77 0.21243911557264994 1101 78-79 0.2093935220123626 1101 80-81 0.19157271158774591 1101 82-83 0.135350542433045 1101 84-85 0.11644999426286518 1101 86-87 0.16086402182744308 1101 88-89 0.06534357309116956 1101 90-91 0.11114418262723547 1101 92-93 0.10302074283410434 1101 94-95 0.05007146072746593 1101 96-97 0.08579845508890571 1101 98-99 0.06063831727625413 1101 100 0.09399707442697292 1102 1 0.21500671707038066 1102 2 0.22526635603500367 1102 3 0.20620673327169214 1102 4 0.22690857116393914 1102 5 0.14618652492802653 1102 6 0.15612369044914942 1102 7 0.2138698789535738 1102 8 0.18955408392654505 1102 9 0.21120922762808902 1102 10-11 0.21398330891465633 1102 12-13 0.24224799178853829 1102 14-15 0.30843545869282707 1102 16-17 0.2846597432713409 1102 18-19 0.2689342395326051 1102 20-21 0.21494650589237807 1102 22-23 0.22942188359725435 1102 24-25 0.2802823731262052 1102 26-27 0.31182418091530195 1102 28-29 0.35678782041104284 1102 30-31 0.322622164618771 1102 32-33 0.4007754690099574 1102 34-35 0.38847472548327744 1102 36-37 0.34677104563998284 1102 38-39 0.34233161181524707 1102 40-41 0.3128750364508406 1102 42-43 0.3155737659852065 1102 44-45 0.3439513085937449 1102 46-47 0.3065869473399019 1102 48-49 0.36136708704369624 1102 50-51 0.3430275897025723 1102 52-53 0.33474712721587707 1102 54-55 0.33442404893030186 1102 56-57 0.28934989288748625 1102 58-59 0.2532782622191405 1102 60-61 0.30165648820938173 1102 62-63 0.30260967804110095 1102 64-65 0.33159734759447446 1102 66-67 0.3252990921315373 1102 68-69 0.3491959153660176 1102 70-71 0.36180511766584544 1102 72-73 0.29524488801558135 1102 74-75 0.29821102376285324 1102 76-77 0.229513025036006 1102 78-79 0.29081000756973907 1102 80-81 0.297954915744036 1102 82-83 0.3152952900386907 1102 84-85 0.23060076043389444 1102 86-87 0.18081607848652226 1102 88-89 0.196911709555323 1102 90-91 0.2089362241676369 1102 92-93 0.21351483090946033 1102 94-95 0.16421862721077218 1102 96-97 0.12249614656417407 1102 98-99 0.1278562142486912 1102 100 0.1559586117636087 1103 1 0.21988770610484565 1103 2 0.28071819468015136 1103 3 0.32768627418769825 1103 4 0.3372316583788262 1103 5 0.24489543227490884 1103 6 0.22738616278449797 1103 7 0.2881613006852106 1103 8 0.29202377281112746 1103 9 0.39619205803199975 1103 10-11 0.39418131169777126 1103 12-13 0.37409293850895153 1103 14-15 0.4550260473931118 1103 16-17 0.4308612539190406 1103 18-19 0.4244589208540006 1103 20-21 0.44058447930490985 1103 22-23 0.4509751320476454 1103 24-25 0.465594930693328 1103 26-27 0.4561721654499138 1103 28-29 0.5124203657577198 1103 30-31 0.46547778239126103 1103 32-33 0.5095805139869043 1103 34-35 0.4981265206260659 1103 36-37 0.460929075977468 1103 38-39 0.46844281938206933 1103 40-41 0.508732484911782 1103 42-43 0.5163225277245758 1103 44-45 0.5022191847789301 1103 46-47 0.5726050388747623 1103 48-49 0.5394127822555888 1103 50-51 0.4973174157709579 1103 52-53 0.456950230067406 1103 54-55 0.4964649227743152 1103 56-57 0.5021155340630372 1103 58-59 0.5133431838759677 1103 60-61 0.5111740465591978 1103 62-63 0.5098678755989141 1103 64-65 0.48270652274467807 1103 66-67 0.5270140355395512 1103 68-69 0.5702518974167532 1103 70-71 0.5455572549465018 1103 72-73 0.5221683108819164 1103 74-75 0.548983315048801 1103 76-77 0.5050948259127992 1103 78-79 0.514342024401607 1103 80-81 0.4358158696912042 1103 82-83 0.4215065443943331 1103 84-85 0.44239467944339594 1103 86-87 0.44187026271341523 1103 88-89 0.4480280251904958 1103 90-91 0.45899438587707664 1103 92-93 0.47522946945498035 1103 94-95 0.4406211244021421 1103 96-97 0.4870210929645893 1103 98-99 0.51871992860681 1103 100 0.5262910805270913 1104 1 0.040790292992703314 1104 2 0.1421265278437467 1104 3 0.1208097228062357 1104 4 0.08156391539490926 1104 5 0.14000345193461072 1104 6 0.16131335974640848 1104 7 0.18431042030206157 1104 8 0.16876062166497263 1104 9 0.24064332825687273 1104 10-11 0.21916367584024954 1104 12-13 0.18580548276444375 1104 14-15 0.24489206463108104 1104 16-17 0.2787350284983958 1104 18-19 0.3050440172159341 1104 20-21 0.2489039835656328 1104 22-23 0.27626280344949805 1104 24-25 0.3004000826493467 1104 26-27 0.23334996645711215 1104 28-29 0.18137672673462646 1104 30-31 0.24137175879292982 1104 32-33 0.2582823459133863 1104 34-35 0.2548890317192587 1104 36-37 0.23739918815505234 1104 38-39 0.28698275074176394 1104 40-41 0.279098810087298 1104 42-43 0.2598027801509559 1104 44-45 0.21150194037499404 1104 46-47 0.2161715050023858 1104 48-49 0.27041980202914573 1104 50-51 0.2847450952797814 1104 52-53 0.20775323104742682 1104 54-55 0.18588755970545634 1104 56-57 0.23590059982556966 1104 58-59 0.23940488167508533 1104 60-61 0.27590744437724624 1104 62-63 0.21316014074581346 1104 64-65 0.2544844680313574 1104 66-67 0.3025374140448278 1104 68-69 0.25513369298779054 1104 70-71 0.26050669104738233 1104 72-73 0.2599892683290079 1104 74-75 0.24357873518336248 1104 76-77 0.27623314581821035 1104 78-79 0.2686442873732773 1104 80-81 0.2817701461877604 1104 82-83 0.25845710081464546 1104 84-85 0.2572156099916256 1104 86-87 0.24091804302093323 1104 88-89 0.2621200173269074 1104 90-91 0.2570751806238931 1104 92-93 0.25801291024641415 1104 94-95 0.2625957138407813 1104 96-97 0.2649365913577313 1104 98-99 0.31207445347470397 1104 100 0.2684947513457736 1105 1 0.12272043479898187 1105 2 0.16010267597325267 1105 3 0.16239327717445207 1105 4 0.15215964408292848 1105 5 0.19461972479192724 1105 6 0.1481878853803522 1105 7 0.13911552607667943 1105 8 0.13084440093290794 1105 9 0.11830409604919367 1105 10-11 0.17283533290623865 1105 12-13 0.22401609615225482 1105 14-15 0.3183222102467198 1105 16-17 0.33159604062182524 1105 18-19 0.3022514291945626 1105 20-21 0.2718931652272758 1105 22-23 0.3075676160519265 1105 24-25 0.30075168955615794 1105 26-27 0.32311180353828917 1105 28-29 0.29922991045435765 1105 30-31 0.2929886660738106 1105 32-33 0.35684670832139886 1105 34-35 0.34397265617215567 1105 36-37 0.4027650535041474 1105 38-39 0.3661121680462429 1105 40-41 0.3432481532625218 1105 42-43 0.39087715283385904 1105 44-45 0.3510419880947069 1105 46-47 0.32582041661128613 1105 48-49 0.32613775433738823 1105 50-51 0.3354191324142306 1105 52-53 0.3774192632905198 1105 54-55 0.36669528143249863 1105 56-57 0.3215488543027192 1105 58-59 0.30456958364061393 1105 60-61 0.26367635413102875 1105 62-63 0.24526432556699262 1105 64-65 0.29168826593330976 1105 66-67 0.29293577538724946 1105 68-69 0.25362223079493873 1105 70-71 0.27211874306848927 1105 72-73 0.3237959917099005 1105 74-75 0.32127609494723686 1105 76-77 0.3392262951235878 1105 78-79 0.3349456556556021 1105 80-81 0.3132260754222713 1105 82-83 0.28881075885419705 1105 84-85 0.2645437680795908 1105 86-87 0.3140854542865057 1105 88-89 0.31559315166707336 1105 90-91 0.3007079815577214 1105 92-93 0.3079977505040077 1105 94-95 0.34557438908714744 1105 96-97 0.3930640787630111 1105 98-99 0.3594011795421146 1105 100 0.30675839979241815 1106 1 -0.0329191919099614 1106 2 0.03314761233329122 1106 3 0.008706212034159222 1106 4 0.012777676836158491 1106 5 -0.02088820315972839 1106 6 0.052937481235986183 1106 7 0.11979663928855899 1106 8 0.08193590779458759 1106 9 0.07045699499398239 1106 10-11 0.09293675081983821 1106 12-13 0.15701805364205512 1106 14-15 0.16725320509339525 1106 16-17 0.12521535352465918 1106 18-19 0.14459809344709385 1106 20-21 0.1555753789768275 1106 22-23 0.15306710037415172 1106 24-25 0.1317955546442846 1106 26-27 0.14728216048278853 1106 28-29 0.16384848956420228 1106 30-31 0.1734313134957972 1106 32-33 0.17725650754678668 1106 34-35 0.1566644675286213 1106 36-37 0.1715773190232781 1106 38-39 0.13968640372564067 1106 40-41 0.15075640857968864 1106 42-43 0.1229236495754904 1106 44-45 0.09435200994781212 1106 46-47 0.09247921912474055 1106 48-49 0.16383905604744342 1106 50-51 0.1583415484437296 1106 52-53 0.12441402747418806 1106 54-55 0.15093432020547937 1106 56-57 0.17795057281288962 1106 58-59 0.1553011706087517 1106 60-61 0.1758435024449554 1106 62-63 0.17674637837585294 1106 64-65 0.16045660477307422 1106 66-67 0.13934796394185156 1106 68-69 0.15646869923777018 1106 70-71 0.10833526836140805 1106 72-73 0.1663486374509091 1106 74-75 0.11734775305524181 1106 76-77 0.10931058216942802 1106 78-79 0.1699853373972715 1106 80-81 0.18615272249232362 1106 82-83 0.11226494532694176 1106 84-85 0.10956216569029209 1106 86-87 0.08816610686501036 1106 88-89 0.11683224978907703 1106 90-91 0.09722343763177577 1106 92-93 0.10396626605014347 1106 94-95 0.08325740728328412 1106 96-97 0.07799404423945333 1106 98-99 0.05148695220520949 1106 100 0.08720448529980018 1107 1 0.08748658215820626 1107 2 0.10576988721932779 1107 3 0.15204046993285658 1107 4 0.1394975688923168 1107 5 0.09930721497669026 1107 6 0.0910287923297588 1107 7 0.17460596404512074 1107 8 0.12386634308572297 1107 9 0.06445355241204709 1107 10-11 0.08725325386926386 1107 12-13 0.07839039277745741 1107 14-15 0.1503660935859159 1107 16-17 0.10780410571235421 1107 18-19 0.14464618692920794 1107 20-21 0.11854694947428612 1107 22-23 0.1462527587950362 1107 24-25 0.13982992488693924 1107 26-27 0.15477564100139318 1107 28-29 0.19539234104706793 1107 30-31 0.16857135878944263 1107 32-33 0.1491244563816707 1107 34-35 0.12398891140836099 1107 36-37 0.20280052123117542 1107 38-39 0.15042489868914544 1107 40-41 0.17003493665377079 1107 42-43 0.1777117645033215 1107 44-45 0.1806579750518651 1107 46-47 0.19118656773955678 1107 48-49 0.18601679682473105 1107 50-51 0.1866351584148802 1107 52-53 0.14714117988144437 1107 54-55 0.14568326251186647 1107 56-57 0.19547388983085057 1107 58-59 0.18504044429352007 1107 60-61 0.20245031115515388 1107 62-63 0.26628040346456316 1107 64-65 0.21515263173991173 1107 66-67 0.22420714843767797 1107 68-69 0.1746113454885858 1107 70-71 0.18983820687763853 1107 72-73 0.19535691477924644 1107 74-75 0.19233157874587903 1107 76-77 0.12032591063216103 1107 78-79 0.13332636546436305 1107 80-81 0.15511539388622708 1107 82-83 0.10715994394210071 1107 84-85 0.12238824837833562 1107 86-87 0.16251020882712197 1107 88-89 0.17445382583414926 1107 90-91 0.17839506052946774 1107 92-93 0.24519764593702575 1107 94-95 0.1602927226645008 1107 96-97 0.17349468789465305 1107 98-99 0.22448561834265135 1107 100 0.22936240745228886 1108 1 -0.04603315919652928 1108 2 -0.013621851055756196 1108 3 0.004565541760214842 1108 4 -0.03591415656762109 1108 5 -0.06003727755192756 1108 6 -0.055413187867699776 1108 7 -0.054133605669214546 1108 8 -0.04006332913156285 1108 9 -0.028959421935695673 1108 10-11 -0.008907211406807392 1108 12-13 -0.004098784311686643 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2214 82-83 -0.10794531621375825 2214 84-85 -0.09519716694989455 2214 86-87 -0.0823496554892742 2214 88-89 -0.08218203656411305 2214 90-91 -0.0882410033824712 2214 92-93 -0.09108433340087885 2214 94-95 -0.10659193170481984 2214 96-97 -0.15493816302404895 2214 98-99 -0.12556270509204026 2214 100 -0.17853320573162534 2215 1 -0.17476147976808676 2215 2 -0.182060862719009 2215 3 -0.16807001738000693 2215 4 -0.18069133845898477 2215 5 -0.12768388815760545 2215 6 -0.15629189745750693 2215 7 -0.20088419544567415 2215 8 -0.2600090064273459 2215 9 -0.32200012301897374 2215 10-11 -0.2751366765374783 2215 12-13 -0.27716863715296824 2215 14-15 -0.3936103679079537 2215 16-17 -0.36805696247598263 2215 18-19 -0.3616944650049021 2215 20-21 -0.38135454597009044 2215 22-23 -0.36292170069170027 2215 24-25 -0.3428426168709038 2215 26-27 -0.3621747324856912 2215 28-29 -0.38132708953686745 2215 30-31 -0.3418946474939091 2215 32-33 -0.39681623253206766 2215 34-35 -0.36896484811458663 2215 36-37 -0.38854594534877407 2215 38-39 -0.38760761433550783 2215 40-41 -0.38056275720525434 2215 42-43 -0.40475337241910125 2215 44-45 -0.4100230720194986 2215 46-47 -0.3261002282604366 2215 48-49 -0.3413078188965315 2215 50-51 -0.38793810587593924 2215 52-53 -0.3886859271145582 2215 54-55 -0.44839842486377535 2215 56-57 -0.40338851544379395 2215 58-59 -0.46580556872705614 2215 60-61 -0.4125968173151122 2215 62-63 -0.4107892484902038 2215 64-65 -0.4012294458124117 2215 66-67 -0.3812123995899981 2215 68-69 -0.36540868532375015 2215 70-71 -0.43945596838770484 2215 72-73 -0.46446493199129435 2215 74-75 -0.4167248561169643 2215 76-77 -0.3884650523982387 2215 78-79 -0.3524439596149236 2215 80-81 -0.41087904152429644 2215 82-83 -0.4144447766482884 2215 84-85 -0.35656057457305934 2215 86-87 -0.44295405670673915 2215 88-89 -0.3800868176935097 2215 90-91 -0.3235457943212836 2215 92-93 -0.34022745013026423 2215 94-95 -0.3383326798273032 2215 96-97 -0.399854297661868 2215 98-99 -0.4508990440020959 2215 100 -0.3948985823269666 2216 1 -0.05195412821091594 2216 2 -0.1742728394773252 2216 3 -0.18803730945760577 2216 4 -0.1692255243355021 2216 5 -0.1198862804838825 2216 6 -0.2037390716966101 2216 7 -0.16484934754944902 2216 8 -0.2321791407068261 2216 9 -0.2716757832367165 2216 10-11 -0.3313547192148789 2216 12-13 -0.35693304892225086 2216 14-15 -0.4228796982256142 2216 16-17 -0.4219776832923472 2216 18-19 -0.40304510923919423 2216 20-21 -0.43061671239463095 2216 22-23 -0.3903625656856846 2216 24-25 -0.4468247344313241 2216 26-27 -0.4943644262346112 2216 28-29 -0.4862585284660099 2216 30-31 -0.49079598390490276 2216 32-33 -0.5365986468426911 2216 34-35 -0.5241257872085185 2216 36-37 -0.5649004778555948 2216 38-39 -0.5430881429185277 2216 40-41 -0.5166503079127551 2216 42-43 -0.4967973137006254 2216 44-45 -0.5218847419791359 2216 46-47 -0.5131559453828274 2216 48-49 -0.5268121735391347 2216 50-51 -0.49667899513602265 2216 52-53 -0.5052626461272567 2216 54-55 -0.5181345716659251 2216 56-57 -0.49196252369577564 2216 58-59 -0.4542324320945994 2216 60-61 -0.5265847132905748 2216 62-63 -0.5044989655054479 2216 64-65 -0.48360419860851067 2216 66-67 -0.4877010774267063 2216 68-69 -0.5137815841466349 2216 70-71 -0.535106236539292 2216 72-73 -0.5832679569941703 2216 74-75 -0.49851498024301577 2216 76-77 -0.4842038861951963 2216 78-79 -0.42895247621401467 2216 80-81 -0.4548467850387681 2216 82-83 -0.4684457291355528 2216 84-85 -0.47327231409138193 2216 86-87 -0.45468633394263946 2216 88-89 -0.304969156863919 2216 90-91 -0.3638255915037192 2216 92-93 -0.4420093695198979 2216 94-95 -0.4026168243808961 2216 96-97 -0.33685393935156327 2216 98-99 -0.32440952134757595 2216 100 -0.37846015380258535 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 44112.0 3 6315.0 4 4151.0 5 3700.0 6 3077.0 7 3753.0 8 4768.0 9 5061.0 10 5251.0 11 5696.0 12 6519.0 13 7219.0 14 8120.0 15 8286.0 16 7886.0 17 7907.0 18 7630.0 19 7438.0 20 7591.0 21 7940.0 22 9414.0 23 13683.0 24 21622.0 25 29505.0 26 31054.0 27 26094.0 28 27196.0 29 34342.0 30 42249.0 31 50574.0 32 65862.0 33 89304.0 34 129123.0 35 199601.0 36 363276.0 37 815584.0 38 1548063.0 39 788662.0 40 1582.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.426749990665634 20.569022779894816 13.543013870188922 23.461213359250625 2 14.853570978712686 20.795732639951055 41.17271426771993 23.177982113616324 3 17.859345583124707 26.214036200540285 30.567098310986353 25.359519905348655 4 11.68102664874368 17.324292662912917 40.715790221895915 30.27889046644749 5 12.199756667519162 38.799609246454324 36.510713894491595 12.489920191534917 6 29.468742795125706 39.55272140402967 18.476306155844593 12.502229645000028 7 26.105945574384513 33.24905174781384 23.604197299435356 17.040805378366287 8 24.119122204875097 37.14888656110986 21.213697087669523 17.51829414634552 9 24.35423955841518 17.398603852395684 22.071098484976286 36.17605810421285 10-11 22.914169007811815 27.65942467435777 29.954305495261853 19.472100822568564 12-13 23.672762013798177 25.084170672718713 29.76559745517055 21.477469858312563 14-15 20.945070489537258 27.256183080149356 26.839263701844164 24.959482728469222 16-17 20.520292514685675 30.614323125128905 27.42616451330736 21.439219846878057 18-19 20.485084446123842 29.735865479990014 29.26327532652726 20.51577474735888 20-21 21.768055983213852 28.895398541670836 28.659424877874663 20.677120597240652 22-23 21.288522903607525 28.695878661948974 28.753099162632857 21.262499271810647 24-25 21.279606851311804 28.88965387949014 28.62256819490721 21.208171074290846 26-27 20.94696386944814 28.788966591902632 28.819544722118007 21.444524816531217 28-29 20.925254393621824 28.900910245940263 28.910221649643365 21.263613710794548 30-31 21.262958089996907 29.290701282378144 28.398658590197144 21.047682037427805 32-33 20.857592057570916 28.939911088518993 28.99497187918565 21.207524974724443 34-35 21.087565089121053 28.701571068048054 28.985234414175697 21.225629428655193 36-37 21.341028956986225 29.035951644644364 28.505066066910317 21.117953331459095 38-39 21.139509105162368 29.1956151636914 28.733691220554963 20.931184510591265 40-41 20.94565613685953 28.878570928933527 28.932123757640728 21.24364917656622 42-43 20.88967558894445 28.755977616461532 28.916464127890766 21.437882666703256 44-45 21.0623272950568 28.880281523818518 28.838927933186454 21.218463247938224 46-47 21.09481924508868 29.133014574053952 28.684803622037556 21.087362558819812 48-49 21.076238378775983 28.907936736452122 28.781191443989805 21.234633440782087 50-51 20.943352510136712 28.8403713888842 28.801185475117958 21.415090625861126 52-53 21.077658277664217 28.865047352772926 28.764776582326746 21.292517787236108 54-55 21.157193560534317 29.184053310994447 28.57767089224922 21.081082236222016 56-57 21.095350609550355 28.839393445882777 28.60099892657233 21.464257017994534 58-59 20.92392636877606 28.593013248563643 28.924857173834706 21.558203208825596 60-61 21.16040928762005 28.937668089869266 28.641540878207817 21.26038174430287 62-63 20.935789534180273 28.99158411593404 28.624724045175782 21.44790230470991 64-65 20.867850528968127 28.78170091712701 28.75894875330964 21.591499800595223 66-67 20.85126665311815 28.75949094320724 28.548109357889214 21.841133045785398 68-69 21.20094476396815 28.92016483546763 28.507974946502472 21.370915454061745 70-71 21.199086923657674 28.655828318075095 28.579150058948237 21.565934699318994 72-73 20.93833522367195 28.692652309090327 28.811954512660957 21.557057954576766 74-75 20.960806733697286 28.780397395025524 28.656451137834736 21.602344733442447 76-77 20.968256547779376 28.76765048069813 28.539900165940246 21.724192805582252 78-79 21.08247600072247 28.801832175215473 28.644798546423942 21.47089327763811 80-81 21.07640561423571 28.49743426697367 28.750695458899404 21.675464659891215 82-83 20.887168924120424 28.57188003808065 28.906834554422094 21.63411648337683 84-85 20.86175063432488 28.562124588255365 28.75313338664875 21.822991390771005 86-87 21.0173702293725 28.61201239365022 28.646719112875562 21.723898264101713 88-89 20.84507292520321 28.515981758268378 28.689768766449625 21.949176550078786 90-91 20.913429152550304 28.3185236616247 28.741716581411808 22.02633060441319 92-93 20.986867807877335 28.554820104014823 28.60804656308668 21.850265525021157 94-95 20.777304602119347 28.291977071048173 28.83108093822831 22.09963738860417 96-97 20.78594603676307 28.310485132034803 28.814227777728423 22.08934105347371 98-99 20.8731778291232 28.35463533641211 28.60625814892427 22.165928685540422 100 20.9188394336344 28.24884946023255 28.52405236502215 22.308258741110897 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3533.0 1 2035.5 2 466.0 3 371.5 4 311.5 5 258.0 6 247.5 7 251.5 8 265.0 9 284.5 10 276.0 11 279.5 12 315.0 13 352.5 14 408.0 15 500.5 16 666.0 17 953.0 18 1369.0 19 1964.0 20 2895.5 21 4483.5 22 6866.5 23 10935.5 24 16314.0 25 22751.5 26 31708.0 27 43464.0 28 58679.5 29 74383.0 30 88225.0 31 102470.0 32 117368.5 33 131062.5 34 142914.5 35 152611.0 36 160364.0 37 168176.5 38 173836.5 39 175372.0 40 174954.0 41 173097.0 42 169011.5 43 165113.0 44 161540.5 45 155692.5 46 150149.5 47 146333.0 48 144627.5 49 138983.5 50 128933.5 51 119576.0 52 109863.0 53 101165.0 54 94671.5 55 89450.5 56 81168.0 57 71700.0 58 63912.5 59 57315.5 60 50548.5 61 42392.0 62 34853.5 63 28670.0 64 23464.5 65 18629.5 66 14275.0 67 10617.5 68 7941.0 69 6006.0 70 4348.5 71 3150.0 72 2402.0 73 1781.5 74 1302.0 75 1019.0 76 648.0 77 436.5 78 336.5 79 209.5 80 150.0 81 113.5 82 90.5 83 71.0 84 46.5 85 45.0 86 43.5 87 29.0 88 20.5 89 17.0 90 14.5 91 11.0 92 9.5 93 9.0 94 7.5 95 4.0 96 2.0 97 2.5 98 2.0 99 1.5 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07354114550673041 2 0.07574378372789776 3 0.07686757873869743 4 0.07916012056072876 5 0.0773395726432333 6 0.07720471724193734 7 0.07639558483416156 8 0.07617082583200163 9 0.07653044023545753 10-11 0.0759685427300577 12-13 0.074900937469798 14-15 0.07341752805554244 16-17 0.07217011559355481 18-19 0.07147336268685901 20-21 0.07109127238318712 22-23 0.07306915160219454 24-25 0.0732377208538145 26-27 0.07241735049593073 28-29 0.06852901975856387 30-31 0.07395694966072629 32-33 0.07226001919441878 34-35 0.06955167321839158 36-37 0.06652866463934047 38-39 0.06795588430305605 40-41 0.07041699537670733 42-43 0.07537293137433387 44-45 0.07653044023545753 46-47 0.07946354521364467 48-49 0.08271131279485572 50-51 0.08394748730673536 52-53 0.08421719810932728 54-55 0.08423967400954327 56-57 0.081767324985784 58-59 0.08082333717671228 60-61 0.078339750202845 62-63 0.07887917180802884 64-65 0.07819365685144104 66-67 0.07773290089701318 68-69 0.07739576239377328 70-71 0.07553026267584582 72-73 0.07546283497519785 74-75 0.07487846156958201 76-77 0.074900937469798 78-79 0.07522683802292991 80-81 0.07651920228534953 82-83 0.07602473248059767 84-85 0.07510322057174194 86-87 0.07608092223113766 88-89 0.07305791365208655 90-91 0.07032709177584334 92-93 0.06996747737238745 94-95 0.07030461587562736 96-97 0.0694617696175276 98-99 0.06902348956331573 100 0.06823683305575597 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4449210.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.1446062151844 #Duplication Level Percentage of deduplicated Percentage of total 1 71.27240278475779 41.44105793929753 2 16.078336133381537 18.697370461416803 3 5.778417566569414 10.079514418652481 4 2.6899909280652654 6.2563385293909315 5 1.373167087593263 3.9921129787880947 6 0.8000051103922913 2.7909589268336945 7 0.4888249554822253 1.9895774181268329 8 0.34477495789820817 1.6037443327878476 9 0.2311425081735349 1.2095721105606205 >10 0.8897083953697044 8.54031084604749 >50 0.03702290261533875 1.4883737589172912 >100 0.01562676955644925 1.5051253679891785 >500 3.4794021125081456E-4 0.13916571913628215 >1k 2.319599338585412E-4 0.26677719205496025 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.37138503239901E-5 0.0 12-13 0.0 1.12379501079967E-5 0.0 5.61897505399835E-5 0.0 14-15 0.0 6.74277006479802E-5 0.0 1.348554012959604E-4 0.0 16-17 0.0 1.236174511879637E-4 0.0 1.348554012959604E-4 0.0 18-19 0.0 1.348554012959604E-4 0.0 1.573313015119538E-4 0.0 20-21 0.0 1.348554012959604E-4 0.0 2.022831019439406E-4 0.0 22-23 0.0 1.348554012959604E-4 0.0 3.1466260302390767E-4 0.0 24-25 0.0 1.685692516199505E-4 0.0 4.49518004319868E-4 0.0 26-27 0.0 1.798072017279472E-4 0.0 6.405631561558119E-4 0.0 28-29 0.0 1.798072017279472E-4 0.0 0.0017194063665234953 0.0 30-31 0.0 1.798072017279472E-4 0.0 0.006090968958534212 0.0 32-33 0.0 1.798072017279472E-4 0.0 0.015924175303031324 0.0 34-35 0.0 1.798072017279472E-4 0.0 0.030128944239539154 0.0 36-37 0.0 2.584728524839241E-4 0.0 0.05611108488922753 0.0 38-39 0.0 3.3713850323990104E-4 0.0 0.10213049058147403 0.0 40-41 0.0 3.483764533478977E-4 0.0 0.15337554307393897 0.0 42-43 0.0 4.045662038878812E-4 0.0 0.20648609528433137 0.0 44-45 0.0 4.045662038878812E-4 0.0 0.2697782302925688 0.0 46-47 0.0 4.1580415399587793E-4 0.0 0.3505566156688491 0.0 48-49 0.0 4.382800542118713E-4 0.0 0.44194362594707826 0.0 50-51 0.0 4.6075595442786473E-4 0.0 0.5407139694462613 0.0 52-53 0.0 4.7199390453586145E-4 0.0 0.6391022226417724 0.0 54-55 0.0 4.7199390453586145E-4 0.0 0.7489868987977641 0.0 56-57 0.0 4.7199390453586145E-4 0.0 0.8803135837598135 0.0 58-59 0.0 4.7199390453586145E-4 0.0 1.028339862582346 0.0 60-61 0.0 5.057077548598515E-4 0.0 1.1862442995498077 0.0 62-63 0.0 5.169457049678482E-4 0.0 1.3478684980030162 0.0 64-65 0.0 5.169457049678482E-4 0.0 1.5209441676162734 0.0 66-67 0.0 5.394216051838416E-4 0.0 1.7180802884107516 0.0 68-69 0.0 5.394216051838416E-4 0.0 1.941423308857078 0.0 70-71 0.0 5.394216051838416E-4 0.0 2.183466278283111 0.0 72-73 0.0 5.394216051838416E-4 0.0 2.4363988213637926 0.0 74-75 0.0 5.506595552918383E-4 0.0 2.6895785993468504 0.0 76-77 0.0 6.293252060478152E-4 0.0 2.9618067027629627 0.0 78-79 0.0 6.293252060478152E-4 0.0 3.2746262819691587 0.0 80-81 0.0 6.518011062638087E-4 0.0 3.6179231818682416 0.0 82-83 0.0 6.742770064798021E-4 0.0 3.9699744449014545 0.0 84-85 0.0 6.742770064798021E-4 0.0 4.316541588281964 0.0 86-87 0.0 6.742770064798021E-4 0.0 4.683460659308057 0.0 88 0.0 6.742770064798021E-4 0.0 4.981468620271913 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11825 0.0 28.140839 1 TACATGG 11320 0.0 27.403273 2 ACATGGG 12385 0.0 24.17399 3 GTATCAA 9985 0.0 20.24069 1 TCAACGC 9295 0.0 20.175692 4 CAACGCA 9445 0.0 19.805286 5 CATGGGG 7025 0.0 19.736975 4 ATCAACG 9615 0.0 19.5531 3 GAGTACA 8755 0.0 19.541153 1 AACGCAG 10020 0.0 18.903288 6 GTACGGA 370 1.6552804E-10 17.784073 1 TATCAAC 10800 0.0 17.668806 2 AGTACAT 9100 0.0 17.095882 2 CATGGGA 4845 0.0 16.879532 4 ACGCAGA 11040 0.0 16.645916 7 GGTATCA 7665 0.0 16.310768 1 CGCAGAG 11135 0.0 16.250643 8 GGACCGT 530 0.0 15.962343 6 TATACCG 265 8.7861845E-6 15.962343 5 GAACCGT 340 3.6052552E-7 15.205958 6 >>END_MODULE