FastQCFastQC Report
Wed 25 May 2016
SRR1032962_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032962_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4449210
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79910.17960491862600325No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66940.15045367604585982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109350.031.322771
GTATCAA166050.030.7568631
TCAACGC182400.027.4963364
ATCAACG183350.027.3282073
CAACGCA185000.027.0811185
GTACATG115200.026.1838761
AACGCAG191500.026.161626
TATCAAC194500.025.95482
TACATGG112600.025.2369062
ACGCAGA214550.023.2183697
CGCAGAG214400.022.9710738
ACATGGG120700.022.6080553
GAGTACT130050.022.16039312-13
GCAGAGT237450.020.554739
AGAGTAC176600.020.53478410-11
CATGGGG70700.019.5645734
GTACTTT151250.018.93004414-15
AGTACTT141000.018.87357112-13
GAGTACA87850.018.6653041
TACACCG5150.017.3554175
Produced by FastQC (version 0.11.5)