##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032962_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4449210 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.82460639079747 34.0 31.0 34.0 31.0 34.0 2 32.86884210904857 34.0 31.0 34.0 31.0 34.0 3 32.9556919093502 34.0 31.0 34.0 31.0 34.0 4 36.369759125777385 37.0 37.0 37.0 35.0 37.0 5 36.356741758649285 37.0 37.0 37.0 35.0 37.0 6 36.411100172839674 37.0 37.0 37.0 35.0 37.0 7 36.37258412167553 37.0 37.0 37.0 35.0 37.0 8 36.30595678783425 37.0 37.0 37.0 35.0 37.0 9 38.14689079634362 39.0 39.0 39.0 37.0 39.0 10-11 38.15915083801394 39.0 39.0 39.0 37.0 39.0 12-13 38.15430604534288 39.0 39.0 39.0 37.0 39.0 14-15 39.691690097792645 41.0 40.0 41.0 37.0 41.0 16-17 39.71141944300224 41.0 40.0 41.0 37.0 41.0 18-19 39.74812247567546 41.0 40.0 41.0 37.0 41.0 20-21 39.766130279308015 41.0 40.0 41.0 37.0 41.0 22-23 39.727962604597224 41.0 40.0 41.0 37.0 41.0 24-25 39.71531328033516 41.0 40.0 41.0 37.0 41.0 26-27 39.64223682406539 41.0 40.0 41.0 37.0 41.0 28-29 39.586698987011175 41.0 40.0 41.0 37.0 41.0 30-31 39.482247859732404 41.0 39.5 41.0 36.5 41.0 32-33 39.35892304476525 41.0 39.0 41.0 36.0 41.0 34-35 39.26958752227924 41.0 39.0 41.0 35.0 41.0 36-37 39.17925182223361 41.0 39.0 41.0 35.0 41.0 38-39 39.08729246315638 41.0 39.0 41.0 35.0 41.0 40-41 38.993610213948095 41.0 39.0 41.0 35.0 41.0 42-43 38.86717799339658 41.0 39.0 41.0 35.0 41.0 44-45 38.71163779637284 41.0 39.0 41.0 35.0 41.0 46-47 38.506251446886075 41.0 39.0 41.0 35.0 41.0 48-49 38.320452844437554 41.0 39.0 41.0 35.0 41.0 50-51 38.18272277550396 41.0 38.0 41.0 34.0 41.0 52-53 38.065479601996756 40.0 38.0 41.0 34.0 41.0 54-55 37.94150691920588 40.0 38.0 41.0 34.0 41.0 56-57 37.75598713029954 40.0 38.0 41.0 33.5 41.0 58-59 37.611136696177525 40.0 37.0 41.0 33.0 41.0 60-61 37.44508373396626 40.0 37.0 41.0 33.0 41.0 62-63 37.235096455325774 40.0 36.5 41.0 33.0 41.0 64-65 36.96758267197997 39.0 36.0 41.0 32.5 41.0 66-67 36.711977407225106 39.0 35.0 41.0 32.0 41.0 68-69 36.43016782754691 39.0 35.0 41.0 32.0 41.0 70-71 36.10631100802165 37.5 35.0 40.0 32.0 41.0 72-73 35.76358724357807 37.0 35.0 40.0 31.0 41.0 74-75 35.41129728648457 37.0 35.0 39.0 31.0 41.0 76-77 35.04068385173997 36.0 35.0 39.0 31.0 41.0 78-79 34.67411293240822 36.0 35.0 38.5 31.0 40.0 80-81 34.309749820754696 35.0 35.0 37.0 30.5 39.0 82-83 33.97059669019893 35.0 34.0 37.0 30.0 39.0 84-85 33.66524585263451 35.0 34.0 36.5 30.0 38.0 86-87 33.49709543941509 35.0 34.0 36.0 30.0 37.0 88-89 33.28291820345635 35.0 34.0 36.0 30.0 37.0 90-91 33.08710804839511 35.0 34.0 35.5 30.0 36.5 92-93 32.911543959489435 35.0 34.0 35.0 30.0 36.0 94-95 32.80353961714552 35.0 34.0 35.0 30.0 36.0 96-97 32.734285412466484 35.0 34.0 35.0 30.0 36.0 98-99 32.6215710204733 35.0 34.0 35.0 30.0 36.0 100 32.53754846365984 35.0 34.0 35.0 30.0 36.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18817665962232866 1101 2 0.1826514848870957 1101 3 0.1666021733844616 1101 4 0.11803358835187083 1101 5 0.10114482196533459 1101 6 0.114252976897248 1101 7 0.09258959316981219 1101 8 -0.015317214549959601 1101 9 0.06130724158359868 1101 10-11 0.14941596632240106 1101 12-13 0.19481416720062583 1101 14-15 0.23748147152482346 1101 16-17 0.2698194991956342 1101 18-19 0.23450379473283078 1101 20-21 0.249966409955114 1101 22-23 0.23106430709596282 1101 24-25 0.21522615277930868 1101 26-27 0.23307628703559402 1101 28-29 0.19974138957040566 1101 30-31 0.213340644461411 1101 32-33 0.23914678282704216 1101 34-35 0.2372328577124847 1101 36-37 0.1954519928310816 1101 38-39 0.22405185823775753 1101 40-41 0.21693125833211013 1101 42-43 0.235903742393063 1101 44-45 0.19159480939399032 1101 46-47 0.19447932871970153 1101 48-49 0.1310557092612541 1101 50-51 0.1379261679113739 1101 52-53 0.17081417233397644 1101 54-55 0.12136017712084168 1101 56-57 0.1579536422339416 1101 58-59 0.13559295777878333 1101 60-61 0.12691473990894053 1101 62-63 0.12314518762363491 1101 64-65 0.12849062226394636 1101 66-67 0.15061242121782215 1101 68-69 0.16552387204507824 1101 70-71 0.19312941765838332 1101 72-73 0.14250573104157382 1101 74-75 0.19774525320270442 1101 76-77 0.20624567392835758 1101 78-79 0.17776787385676585 1101 80-81 0.1990823184881947 1101 82-83 0.1881224804218249 1101 84-85 0.2166342554437577 1101 86-87 0.18961761379710396 1101 88-89 0.17834846610521282 1101 90-91 0.18237304214697758 1101 92-93 0.1636199087296859 1101 94-95 0.19306062471139285 1101 96-97 0.19547850239773368 1101 98-99 0.22799009951587124 1101 100 0.2173667143241076 1102 1 0.1689192583268948 1102 2 0.17494933429865966 1102 3 0.15028816362480768 1102 4 0.09386011040108144 1102 5 0.1088319551065382 1102 6 0.12016760130357795 1102 7 0.1113727074846409 1102 8 -0.08685743656069889 1102 9 0.03877575556501256 1102 10-11 0.09521967253838426 1102 12-13 0.13691150275676733 1102 14-15 0.18765720940456987 1102 16-17 0.1771412499712568 1102 18-19 0.16832377491204653 1102 20-21 0.15641159458535014 1102 22-23 0.17385926494587522 1102 24-25 0.16937489131373695 1102 26-27 0.15610528926965372 1102 28-29 0.1620112767201931 1102 30-31 0.17587513678170552 1102 32-33 0.18292327427890598 1102 34-35 0.16565765123664988 1102 36-37 0.15532516289607656 1102 38-39 0.1706729747933906 1102 40-41 0.13201750741249896 1102 42-43 0.17360220330157716 1102 44-45 0.20985463316678477 1102 46-47 0.16172717621893185 1102 48-49 0.16218956308694032 1102 50-51 0.18745798352637166 1102 52-53 0.1821144920312463 1102 54-55 0.19929658020037522 1102 56-57 0.20589079618583384 1102 58-59 0.21462913576533538 1102 60-61 0.21330043969948065 1102 62-63 0.23048953487651147 1102 64-65 0.2483070438143784 1102 66-67 0.2600773782577974 1102 68-69 0.23479402692164086 1102 70-71 0.2709848277640532 1102 72-73 0.23625279223401918 1102 74-75 0.23872841510091547 1102 76-77 0.26218992545203434 1102 78-79 0.25108811332599146 1102 80-81 0.22309046390657983 1102 82-83 0.2301608429688713 1102 84-85 0.21967478230304494 1102 86-87 0.21732972539641793 1102 88-89 0.19204274687636058 1102 90-91 0.19522772387833243 1102 92-93 0.20562621155792016 1102 94-95 0.1940383758289812 1102 96-97 0.18663169329592222 1102 98-99 0.2208577260617659 1102 100 0.20081602509639396 1103 1 0.16233482309223035 1103 2 0.2035583008018378 1103 3 0.1659321499635098 1103 4 0.1148865413304847 1103 5 0.14972547463806052 1103 6 0.14291791070657212 1103 7 0.1367905636915978 1103 8 0.04472407977776527 1103 9 0.12762960448640115 1103 10-11 0.15331135550042774 1103 12-13 0.16710780825579974 1103 14-15 0.21792070356979565 1103 16-17 0.23090702065931623 1103 18-19 0.2533376807497518 1103 20-21 0.23985213429141794 1103 22-23 0.2458787854425779 1103 24-25 0.19369916143833876 1103 26-27 0.1740876357854475 1103 28-29 0.2144053283556886 1103 30-31 0.25848619645777404 1103 32-33 0.28533899061063295 1103 34-35 0.27798483096022153 1103 36-37 0.2861088746336691 1103 38-39 0.29935362181970504 1103 40-41 0.3502164306208897 1103 42-43 0.3237027646809949 1103 44-45 0.31636037171671205 1103 46-47 0.2656495024451502 1103 48-49 0.23800067569233363 1103 50-51 0.1952278689932072 1103 52-53 0.25972102309391687 1103 54-55 0.2500908605043506 1103 56-57 0.2914884001828639 1103 58-59 0.2467781287677724 1103 60-61 0.24713941954742324 1103 62-63 0.2860962912347347 1103 64-65 0.33094850865919057 1103 66-67 0.3335980492320303 1103 68-69 0.28700862049453946 1103 70-71 0.3306970927064228 1103 72-73 0.2940658557431419 1103 74-75 0.29959088730652894 1103 76-77 0.2938725166794356 1103 78-79 0.3122236374602849 1103 80-81 0.30428783188381914 1103 82-83 0.2789516523809539 1103 84-85 0.2679919737800418 1103 86-87 0.24632036460441498 1103 88-89 0.26041983626550547 1103 90-91 0.24033575114557948 1103 92-93 0.2459476814881043 1103 94-95 0.2802330832863902 1103 96-97 0.2572587381448699 1103 98-99 0.2330232259627394 1103 100 0.2018599088629216 1104 1 0.1029539799430097 1104 2 0.12281172834649112 1104 3 0.15305853112804613 1104 4 0.0608060695773176 1104 5 0.045203522083404835 1104 6 0.0753004444414529 1104 7 0.06159026176094784 1104 8 0.01183996261212883 1104 9 0.09175471228268606 1104 10-11 0.0901363217071065 1104 12-13 0.1143162791477792 1104 14-15 0.1649275625721387 1104 16-17 0.1766456812732713 1104 18-19 0.15572033939071162 1104 20-21 0.12041710833557318 1104 22-23 0.13344648155820948 1104 24-25 0.13939760333086326 1104 26-27 0.15391910016619192 1104 28-29 0.16465782781187954 1104 30-31 0.16719511486078176 1104 32-33 0.24349371731845082 1104 34-35 0.1567503800180745 1104 36-37 0.18833507674715122 1104 38-39 0.2072700992484755 1104 40-41 0.2461502686473409 1104 42-43 0.29946698128637905 1104 44-45 0.2988361004180007 1104 46-47 0.3126812675126729 1104 48-49 0.2760131851658869 1104 50-51 0.30927309390888524 1104 52-53 0.29851398825906017 1104 54-55 0.2719855100513229 1104 56-57 0.3019725423711819 1104 58-59 0.23960147109175267 1104 60-61 0.21285503265721673 1104 62-63 0.1466400385011184 1104 64-65 0.23190638281367626 1104 66-67 0.2884780987093549 1104 68-69 0.300390596712937 1104 70-71 0.2720262058727343 1104 72-73 0.2853229872454577 1104 74-75 0.2584174117813589 1104 76-77 0.293986551784883 1104 78-79 0.29721902241382736 1104 80-81 0.3183740244391444 1104 82-83 0.2780938559843946 1104 84-85 0.2416970799561753 1104 86-87 0.28263793484219235 1104 88-89 0.3045113717950372 1104 90-91 0.29087773987946264 1104 92-93 0.29146072336735074 1104 94-95 0.27851409574294905 1104 96-97 0.262216032444897 1104 98-99 0.2998678941601014 1104 100 0.3150149965545097 1105 1 0.10740500458108926 1105 2 0.17147039626114946 1105 3 0.13539977970351202 1105 4 0.09876297271156176 1105 5 0.09348502714588136 1105 6 0.08875219167295967 1105 7 0.10644253619201294 1105 8 0.05365969681698601 1105 9 0.1184635969272847 1105 10-11 0.11111438730917911 1105 12-13 0.11601565705468175 1105 14-15 0.14969769849351877 1105 16-17 0.17463922519795005 1105 18-19 0.19110644756344186 1105 20-21 0.1645132078621856 1105 22-23 0.1845886991737089 1105 24-25 0.17085164638127992 1105 26-27 0.14773012330715574 1105 28-29 0.16824357473967666 1105 30-31 0.22368433154627354 1105 32-33 0.24022758613575945 1105 34-35 0.2531199638126864 1105 36-37 0.21728485905562422 1105 38-39 0.27171365015943394 1105 40-41 0.24512888146470857 1105 42-43 0.19826025987303098 1105 44-45 0.1840584323347798 1105 46-47 0.209738886886484 1105 48-49 0.21633927607005177 1105 50-51 0.2560027058675587 1105 52-53 0.23512662647659965 1105 54-55 0.23073152012472775 1105 56-57 0.28442803452875864 1105 58-59 0.2626128431545851 1105 60-61 0.30753159979327194 1105 62-63 0.27778408904374885 1105 64-65 0.28296967656895333 1105 66-67 0.2991447286878852 1105 68-69 0.28888894193773496 1105 70-71 0.2166551635030487 1105 72-73 0.26778746651550733 1105 74-75 0.24843443107939578 1105 76-77 0.22784125599090999 1105 78-79 0.25370401552629573 1105 80-81 0.23377005024184427 1105 82-83 0.2459104884149781 1105 84-85 0.2678553097953227 1105 86-87 0.28187465900936814 1105 88-89 0.26202270794640015 1105 90-91 0.265118789325669 1105 92-93 0.2881896733123881 1105 94-95 0.32816005159041595 1105 96-97 0.30470669342964385 1105 98-99 0.3180128372746367 1105 100 0.3061702724814168 1106 1 0.08740711928388833 1106 2 0.0966609046136071 1106 3 0.09368755898927361 1106 4 -0.006234870660620118 1106 5 0.034665645584105675 1106 6 0.0483265903344261 1106 7 0.03577444262647589 1106 8 0.0033999099927584098 1106 9 0.03798738379523314 1106 10-11 0.05551259355353011 1106 12-13 0.04012638003428748 1106 14-15 0.07555132289535749 1106 16-17 0.06902608832970714 1106 18-19 0.07626634901464513 1106 20-21 0.0986280703246365 1106 22-23 0.08025115738561794 1106 24-25 0.10130235531939036 1106 26-27 0.09885024195286718 1106 28-29 0.07800407495494 1106 30-31 0.10599709188577577 1106 32-33 0.059296228421587216 1106 34-35 0.03863713185240414 1106 36-37 0.023781098054129757 1106 38-39 0.06237248425095743 1106 40-41 0.07939314882424497 1106 42-43 0.04545450775062676 1106 44-45 0.0522935759812313 1106 46-47 0.08618393087821374 1106 48-49 0.06561546917151873 1106 50-51 0.13725991326354858 1106 52-53 0.10306592427615158 1106 54-55 0.06413860179119268 1106 56-57 0.12703045309570626 1106 58-59 0.05656023395491161 1106 60-61 0.03869600107231008 1106 62-63 0.04294754629740538 1106 64-65 0.05824139684277441 1106 66-67 0.03283301631045532 1106 68-69 0.15013817400329543 1106 70-71 0.11857967851636886 1106 72-73 0.09189750514796202 1106 74-75 0.14875955904354754 1106 76-77 0.14888410670370433 1106 78-79 0.05388657972933686 1106 80-81 0.06958383839837978 1106 82-83 0.10847432101319043 1106 84-85 0.09273679219498376 1106 86-87 0.1058551154558458 1106 88-89 0.04312952713419804 1106 90-91 0.08012327366810013 1106 92-93 0.05225134331518433 1106 94-95 0.051046631652361896 1106 96-97 0.056639034057859305 1106 98-99 0.016501121403059926 1106 100 -0.036688752914329825 1107 1 0.05132492289901336 1107 2 0.03234171364560012 1107 3 0.031509298632599325 1107 4 0.015709539662694283 1107 5 0.0369207675008596 1107 6 0.07203258918210054 1107 7 0.01848547243659482 1107 8 0.014903232050599513 1107 9 0.048149069703875114 1107 10-11 0.054227583961299786 1107 12-13 0.033748409181171724 1107 14-15 0.10064230457222578 1107 16-17 0.07257431732464426 1107 18-19 0.10757391027280505 1107 20-21 0.09869907131530198 1107 22-23 0.10034559931369103 1107 24-25 0.10498314853616364 1107 26-27 0.10147010814397817 1107 28-29 0.1543009889684157 1107 30-31 0.15807725114422766 1107 32-33 0.13602132632353658 1107 34-35 0.144055978390206 1107 36-37 0.1808176089593445 1107 38-39 0.177815258915615 1107 40-41 0.1493353345615418 1107 42-43 0.12981068758772807 1107 44-45 0.1794896672253543 1107 46-47 0.20276711078932408 1107 48-49 0.1854318579514569 1107 50-51 0.20363900413858005 1107 52-53 0.1311747775401244 1107 54-55 0.18154106857803498 1107 56-57 0.1796059216089887 1107 58-59 0.17497155814047005 1107 60-61 0.2189200260071118 1107 62-63 0.19327159875026467 1107 64-65 0.17876075909326516 1107 66-67 0.17542469565555052 1107 68-69 0.15134664280756738 1107 70-71 0.18293714546283013 1107 72-73 0.22082751943684542 1107 74-75 0.15583322121433696 1107 76-77 0.18498135882798294 1107 78-79 0.12429561624666974 1107 80-81 0.146728430748702 1107 82-83 0.20970658860791502 1107 84-85 0.2162937392225217 1107 86-87 0.17483001461198455 1107 88-89 0.19975727694828294 1107 90-91 0.2095294089623252 1107 92-93 0.22477274921477175 1107 94-95 0.21942996534198755 1107 96-97 0.19454630102321602 1107 98-99 0.15673422088323008 1107 100 0.22420648145494937 1108 1 0.008978348955103854 1108 2 0.03408612541358025 1108 3 0.022411641860983877 1108 4 -0.0019079094434957256 1108 5 -0.013150184618432093 1108 6 0.006547654323270535 1108 7 0.022292839423613486 1108 8 0.04406964140327574 1108 9 0.047587991047059575 1108 10-11 0.017647150913497 1108 12-13 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26-27 -0.17156811353540746 2214 28-29 -0.1849798155561473 2214 30-31 -0.17959172860341965 2214 32-33 -0.1415838270983798 2214 34-35 -0.15903467783053316 2214 36-37 -0.11595562200960075 2214 38-39 -0.14072984536657174 2214 40-41 -0.15770868980089858 2214 42-43 -0.13392682411458168 2214 44-45 -0.12153763696682063 2214 46-47 -0.09740489877904679 2214 48-49 -0.0892245925411359 2214 50-51 -0.09382651738393122 2214 52-53 -0.0945350543803869 2214 54-55 -0.11685192817280665 2214 56-57 -0.09249241524286589 2214 58-59 -0.03483585626489116 2214 60-61 -0.0607767425901784 2214 62-63 -0.0758723142331732 2214 64-65 -0.11196384537353055 2214 66-67 -0.14586771173041768 2214 68-69 -0.1333912693035515 2214 70-71 -0.12414128992124063 2214 72-73 -0.133130026982748 2214 74-75 -0.1627996041337596 2214 76-77 -0.11288838546482793 2214 78-79 -0.09047726019481672 2214 80-81 -0.0915586438112399 2214 82-83 -0.10816458335804668 2214 84-85 -0.16847487810790085 2214 86-87 -0.15597440748607738 2214 88-89 -0.11379851382040584 2214 90-91 -0.12044644469001753 2214 92-93 -0.13903873052974802 2214 94-95 -0.10721596021658542 2214 96-97 -0.09959797343249477 2214 98-99 -0.08426770966331532 2214 100 -0.11553276668045243 2215 1 -0.15702155579192123 2215 2 -0.18533883205265056 2215 3 -0.1631682549931952 2215 4 -0.1090148736351324 2215 5 -0.14166900566027607 2215 6 -0.13893216525768537 2215 7 -0.1420195767233423 2215 8 -0.09123749536622938 2215 9 -0.12315054432814776 2215 10-11 -0.18674837122737387 2215 12-13 -0.18549974267334335 2215 14-15 -0.27375948233055425 2215 16-17 -0.2762890880657949 2215 18-19 -0.2294281217980938 2215 20-21 -0.23787192454849304 2215 22-23 -0.2271915012832153 2215 24-25 -0.24806090527675906 2215 26-27 -0.2288710157351872 2215 28-29 -0.2600824955413259 2215 30-31 -0.2850667139625287 2215 32-33 -0.30933848437673106 2215 34-35 -0.26175678776731814 2215 36-37 -0.2884796855712537 2215 38-39 -0.2719144001950511 2215 40-41 -0.2624972518633939 2215 42-43 -0.27331828838631367 2215 44-45 -0.29058944385199936 2215 46-47 -0.21574553672981267 2215 48-49 -0.19459907384902664 2215 50-51 -0.1841008202038168 2215 52-53 -0.17712982232943375 2215 54-55 -0.16629257374997053 2215 56-57 -0.19062916369157534 2215 58-59 -0.21132316738034973 2215 60-61 -0.17131825199187034 2215 62-63 -0.15319213154354827 2215 64-65 -0.18059235395483597 2215 66-67 -0.2217888831001673 2215 68-69 -0.232085066299355 2215 70-71 -0.24816843952901024 2215 72-73 -0.27986275226420076 2215 74-75 -0.25032717423309236 2215 76-77 -0.2345283630373416 2215 78-79 -0.2388133015145968 2215 80-81 -0.1752666583265423 2215 82-83 -0.18770602689107108 2215 84-85 -0.1878097879420224 2215 86-87 -0.28005733755103535 2215 88-89 -0.27899220785507595 2215 90-91 -0.29909898730075923 2215 92-93 -0.27783010009046905 2215 94-95 -0.303371989755604 2215 96-97 -0.3023110063461303 2215 98-99 -0.29890245091333867 2215 100 -0.34008948333264755 2216 1 -0.18990665285012653 2216 2 -0.2056982473866853 2216 3 -0.20101969248018747 2216 4 -0.12302932267962063 2216 5 -0.1284022868762662 2216 6 -0.13295233257960604 2216 7 -0.13079032315167893 2216 8 -0.12484980939824908 2216 9 -0.1719229722966631 2216 10-11 -0.17915169690675015 2216 12-13 -0.22855999370913338 2216 14-15 -0.3293382014682109 2216 16-17 -0.28149547298023236 2216 18-19 -0.2860662459394092 2216 20-21 -0.24501613561370306 2216 22-23 -0.28209375901400335 2216 24-25 -0.29109427508875285 2216 26-27 -0.31145817284490107 2216 28-29 -0.2786859431786226 2216 30-31 -0.25960832295996994 2216 32-33 -0.34503026598502373 2216 34-35 -0.328350317844901 2216 36-37 -0.3101446870332083 2216 38-39 -0.28195198215532 2216 40-41 -0.2839316053417065 2216 42-43 -0.2800782692567694 2216 44-45 -0.3024270703314471 2216 46-47 -0.3086959657871091 2216 48-49 -0.35699120670435036 2216 50-51 -0.27884757131988636 2216 52-53 -0.24906743944418963 2216 54-55 -0.24568926425570936 2216 56-57 -0.2898926687562451 2216 58-59 -0.3187695945252642 2216 60-61 -0.35141280913603623 2216 62-63 -0.28096565005562724 2216 64-65 -0.292159804980777 2216 66-67 -0.26936335825710955 2216 68-69 -0.32856682368461776 2216 70-71 -0.3555054046537407 2216 72-73 -0.30566585032850213 2216 74-75 -0.3157843058152636 2216 76-77 -0.318856713651428 2216 78-79 -0.33455499040255177 2216 80-81 -0.33198813233180857 2216 82-83 -0.31120587896121776 2216 84-85 -0.27118328047927776 2216 86-87 -0.24807358187674566 2216 88-89 -0.20987232702238146 2216 90-91 -0.24146239808526104 2216 92-93 -0.24397291321638903 2216 94-95 -0.294550054942988 2216 96-97 -0.2626883571449241 2216 98-99 -0.25100668044080265 2216 100 -0.24900843775120052 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 4.0 4 17.0 5 19.0 6 81.0 7 226.0 8 481.0 9 901.0 10 1667.0 11 2883.0 12 4749.0 13 7397.0 14 8814.0 15 11627.0 16 14231.0 17 20155.0 18 24422.0 19 13202.0 20 8685.0 21 8009.0 22 8282.0 23 8906.0 24 9858.0 25 11771.0 26 15002.0 27 19297.0 28 24591.0 29 31709.0 30 40193.0 31 51510.0 32 65898.0 33 85962.0 34 120059.0 35 180081.0 36 325402.0 37 767070.0 38 1618823.0 39 933814.0 40 3404.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.27603665288965 20.63238798714106 13.780952481559039 23.310622878410246 2 15.102219798808186 20.976618874414584 40.73881363044529 23.182347696331938 3 17.84053154652763 26.336137279325794 30.901760898461433 24.921570275685145 4 11.753579068822825 17.393839521677393 40.49485772626824 30.35772368323153 5 11.804173749418153 38.497353113117924 36.680684951796835 13.017788185667095 6 29.182022903259814 39.73126014680401 18.120722552466688 12.965994397469489 7 25.74002400442329 33.46951573098863 23.611923994983343 17.178536269604734 8 24.391737611199577 36.5080772671772 21.080615121454414 18.019570000168805 9 24.59871004631894 16.584396307431167 22.24983547426566 36.567058171984236 10-11 23.047732069288703 27.22113588704512 29.85207486272844 19.87905718093774 12-13 24.197772050409927 24.636974307533283 29.98840466483548 21.17684897722131 14-15 21.18966978565365 26.613913374558713 27.621712302950574 24.574704536837064 16-17 20.10350152049465 30.1811445177908 28.133185441909912 21.58216851980464 18-19 19.945626222760996 28.781735876994613 30.88983230369211 20.38280559655228 20-21 21.265696606813343 27.943331512785413 30.485187257962647 20.30578462243859 22-23 20.673996006693795 27.776628381468065 30.68769398872223 20.86168162311591 24-25 20.67497447007495 28.02945839248735 30.503796139994456 20.791770997443244 26-27 20.393944516047245 27.91543397215418 30.721380845624314 20.96924066617426 28-29 20.41719287901928 28.028513413624434 30.7747265671931 20.77956714016319 30-31 20.72336536161749 28.400127861370223 30.24636242494214 20.630144352070147 32-33 20.380475252372378 28.053238480531306 30.809139345925246 20.757146921171067 34-35 20.579386115776053 27.90303434972921 30.723032686086327 20.79454684840841 36-37 20.915769874762034 28.189266916883284 30.17661383757984 20.718349370774842 38-39 20.698907051298264 28.43249581634999 30.276269864959595 20.59232726739215 40-41 20.588936049257867 28.181421887935677 30.336594156334428 20.893047906472027 42-43 20.57590010960037 28.055647207822133 30.233991047991378 21.134461634586124 44-45 20.806126945481026 28.189322147369385 30.002991338617846 21.001559568531746 46-47 20.926889022946593 28.521334263686803 29.650584167325018 20.90119254604158 48-49 20.984927305095393 28.42264342325769 29.488395095951514 21.104034175695404 50-51 20.89901037373318 28.399988884522454 29.378184660258466 21.3228160814859 52-53 21.000813819771206 28.496978156915343 29.26370573069068 21.238502292622773 54-55 21.027685300431585 28.78921574197634 29.11532753556532 21.067771422026755 56-57 20.930136549285667 28.401841955237106 29.171565603456774 21.496455892020457 58-59 20.775671968402172 28.157766421067016 29.53399055282136 21.532571057709447 60-61 20.985940286570916 28.46806545789538 29.288398191511078 21.25759606402262 62-63 20.80237820008749 28.500798781070095 29.27352934129891 21.423293677543505 64-65 20.76202292092304 28.35305593577287 29.32366644865044 21.56125469465366 66-67 20.723409324351962 28.27501961022294 29.2110509506182 21.7905201148069 68-69 21.10087196388482 28.488498911997738 29.067950160584704 21.342678963532734 70-71 21.13351583764309 28.256083664290966 29.066856812782493 21.543543685283456 72-73 20.95269722040542 28.31371187244477 29.221299961116692 21.512290946033115 74-75 20.93929034592658 28.39650185089038 29.087793113833694 21.576414689349345 76-77 20.94070868094658 28.387694488200655 28.927723003378464 21.743873827474296 78-79 21.10385239263878 28.452091146988153 29.005504124440996 21.438552335932073 80-81 21.095370724574227 28.223103178409808 29.031303562927857 21.650222534088105 82-83 20.82331282331597 28.25661331185236 29.29004443037952 21.630029434452155 84-85 20.801153463199086 28.20433290404364 29.057585503943393 21.93692812881388 86-87 20.889413430049295 28.245376478966516 28.97915222346053 21.886057867523665 88-89 20.67287220802053 28.186657565395812 29.003704152806954 22.13676607377671 90-91 20.665505560095692 28.02016945053542 29.071402435132214 22.242922554236667 92-93 20.769635165906113 28.232595136219697 28.914867999064096 22.082901698810094 94-95 20.51227301808281 27.97677679468566 29.117461333522733 22.3934888537088 96-97 20.44223885541722 27.99936932510594 29.110085359127325 22.448306460349517 98-99 20.515547877785455 28.05737245175181 28.884283575382003 22.542796095080732 100 20.604961330213676 27.872476237354498 28.801337765580854 22.72122466685097 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 61.0 1 60.0 2 178.5 3 302.0 4 203.5 5 162.0 6 386.0 7 832.5 8 1462.0 9 1816.5 10 1570.5 11 1346.0 12 1526.0 13 1877.0 14 2213.0 15 2475.0 16 2824.5 17 3282.5 18 3831.0 19 4545.0 20 5612.5 21 7129.0 22 9572.0 23 13527.0 24 18437.0 25 24570.0 26 33268.5 27 44920.5 28 59299.5 29 74241.5 30 88002.5 31 102342.0 32 116972.5 33 129945.5 34 141482.5 35 150978.0 36 158656.0 37 166840.0 38 172655.5 39 173849.5 40 173095.5 41 171499.0 42 167976.0 43 164775.0 44 161154.5 45 155267.0 46 149588.5 47 145692.0 48 144930.5 49 138914.0 50 128349.0 51 119136.0 52 108883.0 53 100202.0 54 93780.5 55 87655.0 56 79962.5 57 71445.5 58 63531.5 59 56680.5 60 49727.0 61 41589.0 62 34043.5 63 28037.0 64 22735.5 65 17591.5 66 13482.0 67 10023.5 68 7278.0 69 5551.0 70 3962.0 71 2766.0 72 2117.0 73 1481.5 74 1097.0 75 827.0 76 444.0 77 275.0 78 194.5 79 108.0 80 62.5 81 31.5 82 16.0 83 7.5 84 2.0 85 2.0 86 3.0 87 2.0 88 2.5 89 3.0 90 1.0 91 0.0 92 0.5 93 0.5 94 0.5 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.472349023759274E-4 2 5.169457049678482E-4 3 8.99036008639736E-5 4 0.01117052240734872 5 4.719939045358614E-4 6 0.003348909132183017 7 6.74277006479802E-4 8 0.13901344283591918 9 0.0013036022125276173 10-11 0.0 12-13 1.573313015119538E-4 14-15 2.0228310194394064E-4 16-17 0.0 18-19 1.4609335140395712E-4 20-21 0.0 22-23 3.2590055313190433E-4 24-25 4.3828005421187136E-4 26-27 0.006383155661342126 28-29 0.008012658427001648 30-31 0.014429527938667763 32-33 0.017385108817070893 34-35 0.02562252624623248 36-37 0.035894012644941464 38-39 0.04129946664688788 40-41 0.052290181852508645 42-43 0.06870882696029183 44-45 0.10589520386765292 46-47 0.11285149498450288 48-49 0.10972734485447978 50-51 0.1114017994205713 52-53 0.10646833932316076 54-55 0.11302006423612283 56-57 0.09817473214345916 58-59 0.08917313410695382 60-61 0.09130834462747318 62-63 0.0898923629138656 64-65 0.0 66-67 0.0 68-69 1.12379501079967E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 1.12379501079967E-5 78-79 2.24759002159934E-5 80-81 8.99036008639736E-5 82-83 4.49518004319868E-5 84-85 0.0 86-87 6.855149565877988E-4 88-89 3.2590055313190433E-4 90-91 1.348554012959604E-4 92-93 8.316083079917559E-4 94-95 8.877980585317394E-4 96-97 1.798072017279472E-4 98-99 3.37138503239901E-5 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4449210.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.786925196759476 #Duplication Level Percentage of deduplicated Percentage of total 1 70.23013952651056 39.881536798499354 2 16.740869587314634 19.013250179670834 3 6.027969291857663 10.269295237952528 4 2.684092851458705 6.096855199077693 5 1.3868108657262397 3.937636244702461 6 0.8595892805694033 2.9288059305378598 7 0.5129988966227297 2.039214097797457 8 0.3310563651809673 1.5039738436354142 9 0.23944654977190236 1.2237689979467248 >10 0.9258653767961246 8.734954148333877 >50 0.04159628563550228 1.6230727959565938 >100 0.018416029159619464 1.774662421066946 >500 7.528544802826237E-4 0.2978709112250213 >1k 3.169911325848433E-4 0.34467322332870853 >5k 7.924778314592697E-5 0.33042997026846893 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7991 0.17960491862600325 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6694 0.15045367604585982 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.12379501079967E-5 0.0 14-15 0.0 4.49518004319868E-5 0.0 6.74277006479802E-5 0.0 16-17 0.0 8.99036008639736E-5 0.0 6.74277006479802E-5 0.0 18-19 0.0 8.99036008639736E-5 0.0 8.99036008639736E-5 0.0 20-21 0.0 8.99036008639736E-5 0.0 1.348554012959604E-4 0.0 22-23 0.0 8.99036008639736E-5 0.0 2.472349023759274E-4 0.0 24-25 0.0 1.4609335140395712E-4 0.0 3.37138503239901E-4 0.0 26-27 0.0 1.798072017279472E-4 0.0 5.057077548598515E-4 0.0 28-29 0.0 1.9104515183594392E-4 0.0 0.0016182648155515249 0.0 30-31 0.0 2.022831019439406E-4 0.0 0.0059673515073462476 0.0 32-33 0.0 2.022831019439406E-4 0.0 0.01590169940281533 0.0 34-35 0.0 2.022831019439406E-4 0.0 0.030162658089863143 0.0 36-37 0.0 2.584728524839241E-4 0.0 0.05633584389138746 0.0 38-39 0.0 3.2590055313190433E-4 0.0 0.10231029778320197 0.0 40-41 0.0 3.483764533478977E-4 0.0 0.1538475369784748 0.0 42-43 0.0 4.7199390453586145E-4 0.0 0.20701427893940721 0.0 44-45 0.0 4.7199390453586145E-4 0.0 0.2704187934487246 0.0 46-47 0.0 4.944698047518548E-4 0.0 0.3511747029247889 0.0 48-49 0.0 5.281836550758449E-4 0.0 0.442730282454638 0.0 50-51 0.0 5.394216051838416E-4 0.0 0.5418490024071689 0.0 52-53 0.0 5.394216051838416E-4 0.0 0.640439538704624 0.0 54-55 0.0 5.394216051838416E-4 0.0 0.7508186846653675 0.0 56-57 0.0 5.394216051838416E-4 0.0 0.8827522189332488 0.0 58-59 0.0 5.394216051838416E-4 0.0 1.031565154263341 0.0 60-61 0.0 5.61897505399835E-4 0.0 1.1900314887362027 0.0 62-63 0.0 5.843734056158285E-4 0.0 1.352341202145999 0.0 64-65 0.0 5.843734056158285E-4 0.0 1.526113624665952 0.0 66-67 0.0 6.518011062638087E-4 0.0 1.7242836368703656 0.0 68-69 0.0 6.518011062638087E-4 0.0 1.9488178800281397 0.0 70-71 0.0 6.518011062638087E-4 0.0 2.1920745480658366 0.0 72-73 0.0 6.518011062638087E-4 0.0 2.4460971723069935 0.0 74-75 0.0 6.518011062638087E-4 0.0 2.700299603749879 0.0 76-77 0.0 6.742770064798021E-4 0.0 2.973595312426251 0.0 78-79 0.0 6.742770064798021E-4 0.0 3.2880331564479985 0.0 80-81 0.0 6.967529066957954E-4 0.0 3.6315772912494575 0.0 82-83 0.0 7.079908568037921E-4 0.0 3.984257879488718 0.0 84-85 0.0 7.192288069117888E-4 0.0 4.3323309081837 0.0 86-87 0.0 7.192288069117888E-4 0.0 4.701182906628368 0.0 88 0.0 7.192288069117888E-4 0.0 4.999898858449028 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10935 0.0 31.32277 1 GTATCAA 16605 0.0 30.756863 1 TCAACGC 18240 0.0 27.496336 4 ATCAACG 18335 0.0 27.328207 3 CAACGCA 18500 0.0 27.081118 5 GTACATG 11520 0.0 26.183876 1 AACGCAG 19150 0.0 26.16162 6 TATCAAC 19450 0.0 25.9548 2 TACATGG 11260 0.0 25.236906 2 ACGCAGA 21455 0.0 23.218369 7 CGCAGAG 21440 0.0 22.971073 8 ACATGGG 12070 0.0 22.608055 3 GAGTACT 13005 0.0 22.160393 12-13 GCAGAGT 23745 0.0 20.55473 9 AGAGTAC 17660 0.0 20.534784 10-11 CATGGGG 7070 0.0 19.564573 4 GTACTTT 15125 0.0 18.930044 14-15 AGTACTT 14100 0.0 18.873571 12-13 GAGTACA 8785 0.0 18.665304 1 TACACCG 515 0.0 17.355417 5 >>END_MODULE