FastQCFastQC Report
Wed 25 May 2016
SRR1032961_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032961_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2844232
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41550.037.4421461
TCAACGC41050.035.4950334
ATCAACG42950.034.0330623
CAACGCA43350.033.8268555
GGTATCA25100.032.9566271
AACGCAG45550.032.0898866
TATCAAC51350.029.1980632
GTACATG92250.027.563521
ACGCAGA55750.026.0501217
TACATGG92900.025.8528842
CGCAGAG58300.024.5076228
ACATGGG95200.024.3396173
TACCGTC2206.315531E-821.3635527
CATGGGG63000.020.367664
GCAGAGT68750.020.1671949
GAGTACA66300.019.4240951
TATACCG2201.4898324E-619.2271965
CATGGGA32400.018.2786714
GTATACG2352.798759E-618.0002461
AGTACAT67900.017.1666562
Produced by FastQC (version 0.11.5)