##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032961_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2844232 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.613379288328098 33.0 31.0 34.0 30.0 34.0 2 31.617337826168892 33.0 31.0 34.0 30.0 34.0 3 31.77197078156775 34.0 31.0 34.0 30.0 34.0 4 35.11534502108126 37.0 35.0 37.0 33.0 37.0 5 35.1639018898599 37.0 35.0 37.0 33.0 37.0 6 35.24114136962104 37.0 36.0 37.0 33.0 37.0 7 35.142480289934156 37.0 35.0 37.0 33.0 37.0 8 35.12858761169975 37.0 36.0 37.0 33.0 37.0 9 36.76224900078475 39.0 38.0 39.0 34.0 39.0 10-11 36.783057429914294 39.0 38.0 39.0 34.0 39.0 12-13 36.74829743143316 39.0 38.0 39.0 33.0 39.0 14-15 38.21469732426891 41.0 39.0 41.0 34.0 41.0 16-17 38.23892548146565 41.0 39.0 41.0 34.0 41.0 18-19 38.25113686225315 41.0 39.0 41.0 34.0 41.0 20-21 38.30711172646957 41.0 39.0 41.0 34.0 41.0 22-23 38.295382198076666 41.0 39.0 41.0 34.0 41.0 24-25 38.33354452098141 41.0 39.0 41.0 34.0 41.0 26-27 38.32234343049372 41.0 39.0 41.0 34.0 41.0 28-29 38.311034753845675 41.0 39.0 41.0 34.0 41.0 30-31 38.26553494932903 41.0 39.0 41.0 33.0 41.0 32-33 38.220952264090975 41.0 39.0 41.0 33.0 41.0 34-35 38.15229383538333 41.0 39.0 41.0 33.0 41.0 36-37 38.059743719921585 41.0 38.0 41.0 33.0 41.0 38-39 37.96840359717491 41.0 38.0 41.0 33.0 41.0 40-41 37.89065237997463 40.0 38.0 41.0 33.0 41.0 42-43 37.77306351943161 40.0 38.0 41.0 32.5 41.0 44-45 37.63339172050662 40.0 38.0 41.0 32.0 41.0 46-47 37.511843970534045 40.0 38.0 41.0 32.0 41.0 48-49 37.37780690885975 40.0 38.0 41.0 31.5 41.0 50-51 37.242147968238875 40.0 37.0 41.0 31.0 41.0 52-53 37.09083031904571 40.0 37.0 41.0 31.0 41.0 54-55 36.92962757609084 40.0 36.5 41.0 31.0 41.0 56-57 36.74960270470201 40.0 36.0 41.0 30.0 41.0 58-59 36.54170194273885 39.5 35.5 41.0 30.0 41.0 60-61 36.30632803512513 39.0 35.0 41.0 30.0 41.0 62-63 36.05571714965586 39.0 35.0 41.0 30.0 41.0 64-65 35.78048292122443 38.5 35.0 40.0 29.5 41.0 66-67 35.487809890332436 38.0 35.0 40.0 29.0 41.0 68-69 35.18840147357881 37.0 35.0 40.0 29.0 41.0 70-71 34.85521926481384 37.0 34.0 39.5 29.0 41.0 72-73 34.57135036804311 36.0 34.0 39.0 29.0 41.0 74-75 34.30271475744594 36.0 34.0 39.0 29.0 41.0 76-77 33.979447351692826 35.5 34.0 38.5 28.5 40.0 78-79 33.612365833729456 35.0 34.0 37.0 28.0 39.5 80-81 33.28451546849905 35.0 34.0 37.0 28.0 39.0 82-83 32.958378571087025 35.0 34.0 36.5 27.0 39.0 84-85 32.78730409474333 35.0 34.0 36.0 27.5 37.5 86-87 32.54541067676617 35.0 34.0 36.0 27.0 37.0 88-89 32.30241309429048 35.0 34.0 35.5 27.0 37.0 90-91 32.09554951916721 35.0 33.0 35.0 26.5 36.0 92-93 31.8939539742187 35.0 33.0 35.0 26.0 36.0 94-95 31.707613513946825 35.0 33.0 35.0 25.5 36.0 96-97 31.53134343471278 35.0 33.0 35.0 25.0 36.0 98-99 31.34543859291366 35.0 33.0 35.0 25.0 35.5 100 31.213113416908325 35.0 33.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.40439391401226743 1101 2 0.4072901583419082 1101 3 0.4045848809678141 1101 4 0.40232555673903647 1101 5 0.32418931667133677 1101 6 0.3733275399617142 1101 7 0.406507846200995 1101 8 0.27842497767461083 1101 9 0.3590337293369572 1101 10-11 0.3178459315453708 1101 12-13 0.10319639652898616 1101 14-15 0.1432843328158384 1101 16-17 0.1808155502690667 1101 18-19 0.18883965844067774 1101 20-21 0.18761866487510304 1101 22-23 0.191930111561895 1101 24-25 0.14305521581441383 1101 26-27 0.24183221082727613 1101 28-29 0.2496379582310837 1101 30-31 0.2624678383248522 1101 32-33 0.25079442368738825 1101 34-35 0.3065802563353941 1101 36-37 0.2680755883660453 1101 38-39 0.2839297913296832 1101 40-41 0.3381063784240652 1101 42-43 0.3061732320806314 1101 44-45 0.3645104694853245 1101 46-47 0.36461714178671656 1101 48-49 0.3585012799955365 1101 50-51 0.4054776068230552 1101 52-53 0.3032214897404657 1101 54-55 0.4109378534477983 1101 56-57 0.42796303748338715 1101 58-59 0.379479118751064 1101 60-61 0.4616762398225305 1101 62-63 0.5129266521661435 1101 64-65 0.45407485622890675 1101 66-67 0.3977258712984195 1101 68-69 0.4698892816046225 1101 70-71 0.4998668234263235 1101 72-73 0.41316293838748663 1101 74-75 0.41819021700959524 1101 76-77 0.39935829668921485 1101 78-79 0.35949625777455907 1101 80-81 0.34849050822423067 1101 82-83 0.28762966450752003 1101 84-85 0.3501558605720163 1101 86-87 0.2790660391253539 1101 88-89 0.28044999183600794 1101 90-91 0.3259698652041578 1101 92-93 0.32639146550055287 1101 94-95 0.29156066670406133 1101 96-97 0.2714602064085305 1101 98-99 0.321723016339984 1101 100 0.40652786605495095 1102 1 0.3578070048998434 1102 2 0.32751566301146084 1102 3 0.37247090242114567 1102 4 0.3547959972341488 1102 5 0.30591445697310604 1102 6 0.37387704961140855 1102 7 0.32378721545480715 1102 8 0.3142944990486143 1102 9 0.45126086357758055 1102 10-11 0.41020335079959835 1102 12-13 0.43461968971462994 1102 14-15 0.5555894767209466 1102 16-17 0.4613396886266443 1102 18-19 0.49089873980360466 1102 20-21 0.4415745427594544 1102 22-23 0.48449230282405864 1102 24-25 0.5098823522062901 1102 26-27 0.5002628209483717 1102 28-29 0.5219634179933195 1102 30-31 0.5233418638763112 1102 32-33 0.5698582539323311 1102 34-35 0.5636448906096305 1102 36-37 0.56000995107199 1102 38-39 0.579740570162464 1102 40-41 0.5919795676775053 1102 42-43 0.6047960294034027 1102 44-45 0.5333418976188185 1102 46-47 0.5716996583177192 1102 48-49 0.5907679972165525 1102 50-51 0.5404298222061001 1102 52-53 0.5495097113836422 1102 54-55 0.5292873345934552 1102 56-57 0.5311472220390314 1102 58-59 0.5009297596887876 1102 60-61 0.5699908169542098 1102 62-63 0.5619359348052981 1102 64-65 0.5787867905512698 1102 66-67 0.6000270353929977 1102 68-69 0.6032294720439353 1102 70-71 0.5440035944554538 1102 72-73 0.5410600656634443 1102 74-75 0.5332215983534283 1102 76-77 0.4705662498565317 1102 78-79 0.5673752069970206 1102 80-81 0.500515035151146 1102 82-83 0.524170980587833 1102 84-85 0.4807974436761029 1102 86-87 0.4801703020940309 1102 88-89 0.4982985008860439 1102 90-91 0.5130715816319054 1102 92-93 0.4713580808061515 1102 94-95 0.4460452140140667 1102 96-97 0.5211125107995009 1102 98-99 0.5569856838348848 1102 100 0.4802432343084355 1103 1 0.25506386223783295 1103 2 0.26643197429350707 1103 3 0.31075003044220395 1103 4 0.3148807579085542 1103 5 0.279828130181599 1103 6 0.20738770246232718 1103 7 0.27541860039998056 1103 8 0.32647216814822144 1103 9 0.32502538092423094 1103 10-11 0.35071952499776415 1103 12-13 0.3766916400750233 1103 14-15 0.4034008352062912 1103 16-17 0.4554694400927559 1103 18-19 0.45562236707499437 1103 20-21 0.38291124924813147 1103 22-23 0.3752527824723799 1103 24-25 0.342235079432335 1103 26-27 0.43152755192127046 1103 28-29 0.4562994721277036 1103 30-31 0.4656291585795316 1103 32-33 0.4591937913440276 1103 34-35 0.38850867226505414 1103 36-37 0.33968844063905834 1103 38-39 0.4327220406294643 1103 40-41 0.45388416472003 1103 42-43 0.4330455116757932 1103 44-45 0.41857500049513163 1103 46-47 0.4358624048291233 1103 48-49 0.4523759163386245 1103 50-51 0.49367043850261183 1103 52-53 0.40082697172383774 1103 54-55 0.4627854486528875 1103 56-57 0.4909732972015064 1103 58-59 0.5095051844095551 1103 60-61 0.5388545794189312 1103 62-63 0.5166785803243243 1103 64-65 0.42695771541486494 1103 66-67 0.4401095969163862 1103 68-69 0.4305699891841499 1103 70-71 0.47359708087095953 1103 72-73 0.46306307730740315 1103 74-75 0.4093845647799341 1103 76-77 0.38291763373324983 1103 78-79 0.4106103928427913 1103 80-81 0.4462073508429114 1103 82-83 0.4557978712774542 1103 84-85 0.42326831432595924 1103 86-87 0.42704634712838896 1103 88-89 0.40931314845069977 1103 90-91 0.3948439067426648 1103 92-93 0.3910847247785121 1103 94-95 0.37179517832324294 1103 96-97 0.4010091066148611 1103 98-99 0.4561925696153075 1103 100 0.44205574190205255 1104 1 0.08849493426696498 1104 2 0.16632494623767613 1104 3 0.19970862396698053 1104 4 0.12635437376033565 1104 5 0.11832783324646101 1104 6 0.0838084492314124 1104 7 0.1285837566468757 1104 8 0.14803104135793888 1104 9 0.1618436395745988 1104 10-11 0.13905603305666858 1104 12-13 0.10792849129684612 1104 14-15 0.17033991400880666 1104 16-17 0.17320223541181434 1104 18-19 0.14282526535532014 1104 20-21 0.13891381117759494 1104 22-23 0.15632319691647467 1104 24-25 0.21261677109545474 1104 26-27 0.24410885577427877 1104 28-29 0.23543148680580117 1104 30-31 0.25207273225666427 1104 32-33 0.2735926878275592 1104 34-35 0.2994683278264887 1104 36-37 0.2940955859751284 1104 38-39 0.2936027686557807 1104 40-41 0.26204283649361315 1104 42-43 0.1929085713021479 1104 44-45 0.16355355490417622 1104 46-47 0.1693313367855751 1104 48-49 0.1832568854833454 1104 50-51 0.13740703481456507 1104 52-53 0.1396450130577236 1104 54-55 0.11515275094028965 1104 56-57 0.1409068842690786 1104 58-59 0.12787655918049978 1104 60-61 0.1920498661678991 1104 62-63 0.09076310676500299 1104 64-65 0.13410668375353652 1104 66-67 0.1318565852453304 1104 68-69 0.1698426843603471 1104 70-71 0.19532754484164627 1104 72-73 0.18475353050027365 1104 74-75 0.14775520441870071 1104 76-77 0.1808222342784802 1104 78-79 0.23389688866911484 1104 80-81 0.20747063273206834 1104 82-83 0.2067866271641705 1104 84-85 0.14060760318040622 1104 86-87 0.11701104280814434 1104 88-89 0.16389280191335587 1104 90-91 0.22769328838555225 1104 92-93 0.2235802698216922 1104 94-95 0.23383342961857423 1104 96-97 0.22408147814694956 1104 98-99 0.18339793982409702 1104 100 0.1069049502372117 1105 1 0.11470903125585963 1105 2 0.042426922360107255 1105 3 0.1529948328165851 1105 4 0.12500040278634117 1105 5 0.042115997855148635 1105 6 0.18155141068859137 1105 7 0.11824481994908353 1105 8 0.12283158607731082 1105 9 0.12334569171299847 1105 10-11 0.1281384321092034 1105 12-13 0.1396868484675764 1105 14-15 0.16490855445165664 1105 16-17 0.2038445225543981 1105 18-19 0.17975288174439896 1105 20-21 0.17687695245479063 1105 22-23 0.21739018579557268 1105 24-25 0.11282917006374049 1105 26-27 0.15495969627296802 1105 28-29 0.22005823985016093 1105 30-31 0.24090758048195937 1105 32-33 0.22276789782600304 1105 34-35 0.24708593344055885 1105 36-37 0.2598602864938968 1105 38-39 0.2610873588707321 1105 40-41 0.24775841284219524 1105 42-43 0.23747414350096818 1105 44-45 0.24757417589406572 1105 46-47 0.1901126700550151 1105 48-49 0.19258028419905315 1105 50-51 0.1969937079877795 1105 52-53 0.2853845426227295 1105 54-55 0.23220192834914855 1105 56-57 0.27091316356341366 1105 58-59 0.2372038297990855 1105 60-61 0.2878522753160624 1105 62-63 0.29025585163761036 1105 64-65 0.23987323776655245 1105 66-67 0.27200267907193165 1105 68-69 0.34716104745126586 1105 70-71 0.30558177171376144 1105 72-73 0.3021182261672166 1105 74-75 0.3134166004177459 1105 76-77 0.37683361265379745 1105 78-79 0.3318555533573644 1105 80-81 0.30152614446647874 1105 82-83 0.31751480362876805 1105 84-85 0.3160928473167317 1105 86-87 0.2617503905490679 1105 88-89 0.3203699606441006 1105 90-91 0.30551677347217776 1105 92-93 0.29676138518903983 1105 94-95 0.3102840921053378 1105 96-97 0.32157832281498955 1105 98-99 0.3087903210306209 1105 100 0.3359075701650731 1106 1 -0.027383559930147072 1106 2 -0.06136304817103877 1106 3 -0.04014876287044089 1106 4 -0.09164473162048381 1106 5 -0.0792268550277555 1106 6 -0.05326602095171751 1106 7 -0.09065116961944142 1106 8 -0.11514946013733152 1106 9 -0.046612359276750226 1106 10-11 0.005430242771602423 1106 12-13 0.036548873960555284 1106 14-15 -0.01865053031620789 1106 16-17 -0.028306766932580274 1106 18-19 2.4778097278499445E-4 1106 20-21 0.027864499798631925 1106 22-23 0.021965163788230768 1106 24-25 0.024610402074145554 1106 26-27 0.09582239669794745 1106 28-29 -0.00816631868396911 1106 30-31 0.005207099412061211 1106 32-33 0.06005775878691111 1106 34-35 0.021148513691350956 1106 36-37 0.07143633481295808 1106 38-39 0.06095116108373588 1106 40-41 0.02597877745130006 1106 42-43 0.025002811318053375 1106 44-45 -0.017038294238211904 1106 46-47 -0.021136653993920618 1106 48-49 0.03168441243934694 1106 50-51 0.0029656849902650606 1106 52-53 0.0147273089287836 1106 54-55 0.05144892676475621 1106 56-57 0.04841588186410206 1106 58-59 0.03267902389514177 1106 60-61 0.020378405822860657 1106 62-63 0.08628618821200718 1106 64-65 0.0402541759094035 1106 66-67 0.10998828377591963 1106 68-69 0.09822458492742214 1106 70-71 0.17393719219106174 1106 72-73 0.16287176282870064 1106 74-75 0.11042095526777729 1106 76-77 0.10913463186656713 1106 78-79 0.08817998368542135 1106 80-81 0.059576083578491534 1106 82-83 0.07954897151586948 1106 84-85 0.16307380402652427 1106 86-87 0.1524677848201037 1106 88-89 0.14840258610833956 1106 90-91 0.1330557443937863 1106 92-93 0.14043959005823226 1106 94-95 0.13893773997903125 1106 96-97 0.18117311904104483 1106 98-99 0.24380520874681721 1106 100 0.18968989058657115 1107 1 0.109465304227637 1107 2 0.12135388693442906 1107 3 0.15104437696919604 1107 4 0.1574058167931227 1107 5 0.14410522101132273 1107 6 0.0766199282017439 1107 7 0.07114331980243804 1107 8 0.09139028461897425 1107 9 0.12884175726016878 1107 10-11 0.06326286190995489 1107 12-13 0.09321638962489232 1107 14-15 0.1434355995246932 1107 16-17 0.13111077206298205 1107 18-19 0.17405615442287825 1107 20-21 0.21286522241878458 1107 22-23 0.15036544804042506 1107 24-25 0.18289913062561425 1107 26-27 0.21932571426973624 1107 28-29 0.18827821195642258 1107 30-31 0.1925527730236638 1107 32-33 0.14527930181100857 1107 34-35 0.2106717690983686 1107 36-37 0.2866233800159321 1107 38-39 0.1956160435591272 1107 40-41 0.1673870345044648 1107 42-43 0.21795105940165627 1107 44-45 0.21427290164304935 1107 46-47 0.1708302240796371 1107 48-49 0.20861431050778378 1107 50-51 0.2229806753078023 1107 52-53 0.18254158064432602 1107 54-55 0.18109791807776077 1107 56-57 0.16943037250438664 1107 58-59 0.23201840973930388 1107 60-61 0.2643838180209457 1107 62-63 0.26200934303547996 1107 64-65 0.2903945683666791 1107 66-67 0.27457247582827193 1107 68-69 0.19550813264886102 1107 70-71 0.22078549899159583 1107 72-73 0.2506920650200186 1107 74-75 0.2002410196101394 1107 76-77 0.2271832204565527 1107 78-79 0.25506781100317966 1107 80-81 0.19892908578977142 1107 82-83 0.10925732785639042 1107 84-85 0.09993818993550008 1107 86-87 0.10431787062719167 1107 88-89 0.1354381973477743 1107 90-91 0.2071437523989914 1107 92-93 0.26766893802802727 1107 94-95 0.2150163558665632 1107 96-97 0.21673186319851467 1107 98-99 0.2557327348065108 1107 100 0.23974397368205302 1108 1 0.18343477083409 1108 2 0.18729924294828137 1108 3 0.14752877157002686 1108 4 0.18262032830899955 1108 5 0.1685281724890686 1108 6 0.1414513296769826 1108 7 0.1513248391962847 1108 8 0.18640508716801918 1108 9 0.17975499926930638 1108 10-11 0.1954446071053937 1108 12-13 0.28180274619069934 1108 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14-15 -0.5343644987510174 2215 16-17 -0.5117168210755878 2215 18-19 -0.5214792024966215 2215 20-21 -0.5225818203758195 2215 22-23 -0.5215504145522729 2215 24-25 -0.49488821414344386 2215 26-27 -0.5182229545411232 2215 28-29 -0.5379530263175027 2215 30-31 -0.5394353668852148 2215 32-33 -0.5942376284132465 2215 34-35 -0.5663620243439595 2215 36-37 -0.5452531052873866 2215 38-39 -0.5296239055161891 2215 40-41 -0.525029364137545 2215 42-43 -0.5617463184237721 2215 44-45 -0.5190304575993352 2215 46-47 -0.5220789378965947 2215 48-49 -0.5612961915917438 2215 50-51 -0.5348780310265155 2215 52-53 -0.5268286954210737 2215 54-55 -0.5558676630890389 2215 56-57 -0.5206975422637754 2215 58-59 -0.592917510031981 2215 60-61 -0.5984649937364068 2215 62-63 -0.5539474379883771 2215 64-65 -0.511510126531924 2215 66-67 -0.500473847766834 2215 68-69 -0.5640412508944195 2215 70-71 -0.5319222201185596 2215 72-73 -0.48763338392128475 2215 74-75 -0.3963357694544598 2215 76-77 -0.46387092052441403 2215 78-79 -0.40525332720819307 2215 80-81 -0.4748450811002414 2215 82-83 -0.49615567580661235 2215 84-85 -0.49581272581541924 2215 86-87 -0.541958351118538 2215 88-89 -0.5359185434723841 2215 90-91 -0.5534390225496395 2215 92-93 -0.5574621930360344 2215 94-95 -0.5433978315720189 2215 96-97 -0.4925012289126265 2215 98-99 -0.46560133559463424 2215 100 -0.430209970747498 2216 1 -0.21810107344029106 2216 2 -0.21985762055801317 2216 3 -0.3077923771514257 2216 4 -0.2386740149564659 2216 5 -0.21362215615209834 2216 6 -0.20871883562275428 2216 7 -0.24044075627011807 2216 8 -0.16357740025613765 2216 9 -0.2325632430547131 2216 10-11 -0.3154946061399784 2216 12-13 -0.38982530654027414 2216 14-15 -0.4410509000265108 2216 16-17 -0.4399284581664489 2216 18-19 -0.4409257978618726 2216 20-21 -0.47054696532105567 2216 22-23 -0.4747220309992102 2216 24-25 -0.5263007101690249 2216 26-27 -0.5653090000775691 2216 28-29 -0.6094358407111073 2216 30-31 -0.6326947937398302 2216 32-33 -0.7120259268101137 2216 34-35 -0.6556920276156504 2216 36-37 -0.648621585516679 2216 38-39 -0.605389080874339 2216 40-41 -0.5420040707219442 2216 42-43 -0.5727970665356352 2216 44-45 -0.5476599314096617 2216 46-47 -0.4529664146837007 2216 48-49 -0.5223080572403447 2216 50-51 -0.5404127224966118 2216 52-53 -0.5279802209057252 2216 54-55 -0.5403713932803171 2216 56-57 -0.4923147817839819 2216 58-59 -0.5223248542924637 2216 60-61 -0.45752105028601164 2216 62-63 -0.46959365273126963 2216 64-65 -0.4982399169131426 2216 66-67 -0.5598454245848075 2216 68-69 -0.6537009487005463 2216 70-71 -0.6236774605880271 2216 72-73 -0.6244868074489034 2216 74-75 -0.5678478724160314 2216 76-77 -0.4973947002058168 2216 78-79 -0.506626092895047 2216 80-81 -0.5352692584536811 2216 82-83 -0.5555087836849495 2216 84-85 -0.5632149551749492 2216 86-87 -0.6001234564348152 2216 88-89 -0.5278128723835529 2216 90-91 -0.5134135494213012 2216 92-93 -0.5301643629521209 2216 94-95 -0.4734233139163919 2216 96-97 -0.4422612156415333 2216 98-99 -0.5142556742748035 2216 100 -0.5087218018359607 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 37734.0 3 6142.0 4 3882.0 5 3299.0 6 2631.0 7 3019.0 8 3412.0 9 3922.0 10 4089.0 11 4363.0 12 4878.0 13 5498.0 14 5889.0 15 5943.0 16 5889.0 17 5659.0 18 5418.0 19 5389.0 20 5542.0 21 5843.0 22 6944.0 23 10324.0 24 16461.0 25 21517.0 26 22321.0 27 18645.0 28 19749.0 29 24477.0 30 30322.0 31 36295.0 32 46488.0 33 63552.0 34 91583.0 35 144339.0 36 272503.0 37 569394.0 38 920862.0 39 399419.0 40 596.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.73598100013194 20.17043585345472 13.683599419448475 23.40998372696486 2 15.131944461548585 20.45799764748685 40.6588936854761 23.75116420548847 3 17.913651800756362 26.137462403607564 30.03640358223072 25.912482213405358 4 12.11194089721052 17.161371502242815 39.50106334914869 31.225624251397978 5 12.715313858411676 38.6194086306401 35.50105664504116 13.164220865907064 6 30.04796611970559 38.58295865666715 18.336449676742415 13.03262554688484 7 27.083189656992378 32.877064189193945 22.86617762758811 17.173568526225566 8 24.84404693669092 36.88563958085052 20.570076223390164 17.700237259068395 9 24.87531799453208 17.70181877038596 21.305088264374408 36.11777497070756 10-11 23.499507836353608 27.67884364853798 28.908381911952407 19.913266603156003 12-13 24.116983500793083 24.998399057590294 28.93186177991018 21.952755661706444 14-15 21.509224180168165 26.862547268810545 25.97836652009194 25.64986203092935 16-17 21.366556199002236 30.251474319346666 26.528019720219735 21.853949761431362 18-19 21.34967058678653 29.2442231741536 28.34322305980455 21.062883179255323 20-21 22.41105753541681 28.337617297374894 27.990560794964864 21.260764372243433 22-23 22.136904739914208 28.086897518216535 28.041139575096775 21.735058166772486 24-25 21.90421452703297 28.275897375271015 27.896708867529263 21.923179230166756 26-27 21.699237125749345 28.34771683087357 27.96992132001663 21.983124723360447 28-29 21.769746827693794 28.29126109066959 28.060300029483642 21.878692052152974 30-31 21.91442176052153 28.60533457466853 27.767292576864794 21.71295108794515 32-33 21.70476193994653 28.35908935856845 28.131480018387485 21.804668683097535 34-35 21.88538962107036 28.22581709182879 28.06654072093756 21.82225256616329 36-37 22.04207112852577 28.446854147099955 27.66887655109558 21.8421981732787 38-39 21.8120065103168 28.556010749200105 27.86400812311901 21.76797461736408 40-41 21.62972014396627 28.26264291998416 28.15496200381853 21.95267493223104 42-43 21.69175056701789 28.27484059109196 28.029015552792302 22.004393289097845 44-45 21.723589633881158 28.395585828148068 28.0289990105657 21.851825527405076 46-47 21.773540596165073 28.444796865230014 27.929249513798894 21.852413024806022 48-49 21.7351579534832 28.25422390157622 28.017598497589674 21.99301964735091 50-51 21.667662512967116 28.267431532927677 27.986887906414378 22.078018047690833 52-53 21.77422902102147 28.223782808767233 27.984093934532545 22.017894235678753 54-55 21.821858736687386 28.56769100922248 27.83182106170846 21.778629192381675 56-57 21.71887282828162 28.300023153735882 27.921909979119413 22.05919403886309 58-59 21.68221434721734 27.94002821348934 28.16376913638502 22.213988302908298 60-61 21.875359566103334 28.29341438517759 27.796909675587365 22.034316373131706 62-63 21.62062164083666 28.271380196636354 27.9321408759806 22.175857286546385 64-65 21.711441406603242 28.12017169798242 27.97337196385461 22.195014931559726 66-67 21.53603907128292 28.19429025839334 27.91063288511375 22.359037785209985 68-69 21.910813323321584 28.2380082435079 27.843725215464914 22.007453217705596 70-71 21.866943509963438 28.043877621751413 27.90709212147186 22.182086746813294 72-73 21.52741858869478 28.110307700427505 28.081613623968614 22.280660086909094 74-75 21.648559186434476 28.157804689032506 27.973202512616304 22.220433611916718 76-77 21.692507060014876 28.19683384810043 27.814189134508677 22.296469957376015 78-79 21.69607292755045 28.188684695766636 27.90176187745938 22.213480499223536 80-81 21.62484781950866 28.070879163411938 28.003779002258955 22.300494014820444 82-83 21.600469100403565 28.083217116746077 28.11372342111842 22.202590361731936 84-85 21.568498436965893 28.049024856367815 27.956504215151746 22.425972491514543 86-87 21.766123464922003 28.07525796629039 27.917782943085207 22.240835625702402 88-89 21.49491244779889 28.050640135419417 27.970119624943663 22.484327791838034 90-91 21.490450162638382 27.97710924127303 28.076996828155703 22.455443767932884 92-93 21.66710734572407 28.06932083880664 27.94527979953589 22.318292015933405 94-95 21.482899399425552 27.824106399436634 28.079296445300823 22.61369775583699 96-97 21.558783937356054 27.76533641636719 28.070924790533603 22.60495485574315 98-99 21.489566029215435 27.864682333118303 28.003411405232615 22.642340232433646 100 21.569133814828785 27.686914901402265 28.003775873812913 22.740175409956034 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2715.0 1 1669.5 2 548.0 3 426.5 4 330.0 5 280.0 6 258.5 7 251.5 8 272.0 9 269.0 10 261.0 11 253.5 12 266.5 13 306.0 14 337.5 15 429.0 16 547.0 17 694.5 18 990.5 19 1369.5 20 1965.0 21 2994.5 22 4460.0 23 6715.5 24 10006.0 25 13856.0 26 18822.5 27 25208.0 28 33050.0 29 41512.0 30 49333.5 31 57519.5 32 65569.0 33 72850.0 34 79392.5 35 85159.5 36 90876.5 37 95607.5 38 98579.0 39 99788.0 40 100629.5 41 102032.0 42 102194.5 43 101646.5 44 100427.5 45 98442.0 46 96687.5 47 93854.0 48 91321.5 49 89240.5 50 86502.5 51 83055.0 52 77278.0 53 72607.0 54 70144.0 55 66609.5 56 61030.0 57 54679.5 58 49636.0 59 46364.0 60 42373.0 61 36680.0 62 31734.0 63 27514.5 64 22866.0 65 17896.5 66 14032.5 67 11148.0 68 8309.5 69 6017.5 70 4301.5 71 3140.5 72 2521.5 73 1959.5 74 1519.0 75 1094.5 76 694.5 77 503.0 78 353.5 79 221.5 80 147.0 81 102.0 82 66.5 83 48.0 84 44.0 85 42.5 86 36.5 87 25.5 88 16.5 89 15.0 90 13.0 91 6.5 92 4.0 93 3.5 94 4.0 95 3.5 96 3.0 97 3.0 98 2.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07407975158144624 2 0.0761189663853019 3 0.0774901625465152 4 0.07974033060594213 5 0.07798238680951484 6 0.0782636578169432 7 0.07720889153908682 8 0.07703309715944409 9 0.0774198447946581 10-11 0.07698035884555127 12-13 0.07580253650194499 14-15 0.07416764877126761 16-17 0.07288434979987568 18-19 0.07232180778501895 20-21 0.07184716295998357 22-23 0.07392153663976778 24-25 0.07418522820923187 26-27 0.07334141518694678 28-29 0.06954425658666381 30-31 0.07492356460373134 32-33 0.07318320024526832 34-35 0.07040564904691318 36-37 0.0677335744763437 38-39 0.06940362108294963 40-41 0.07167136858034084 42-43 0.07629476076494462 44-45 0.0774901625465152 46-47 0.08090057351158415 48-49 0.08432856391461738 50-51 0.08536575075450947 52-53 0.08518995637486675 54-55 0.08527785356468812 56-57 0.08290462943951127 58-59 0.08188502203758344 60-61 0.07963485397815649 62-63 0.08035561093469168 64-65 0.07963485397815649 66-67 0.07900199421144267 68-69 0.07868556432808575 70-71 0.07703309715944409 72-73 0.07671666727608718 74-75 0.07645297570662309 76-77 0.07629476076494462 78-79 0.07659361121033728 80-81 0.07777143355394356 82-83 0.0772264709770511 84-85 0.07606622807140909 86-87 0.07713857378722973 88-89 0.07379848057401787 90-91 0.07161863026644802 92-93 0.07128462094512683 94-95 0.07160105082848375 96-97 0.07066934061637728 98-99 0.07037049017098464 100 0.06947393883480672 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2844232.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.96643896679228 #Duplication Level Percentage of deduplicated Percentage of total 1 78.15539427956844 49.99322258109601 2 13.313868516097019 17.032815156936348 3 3.956986454660532 7.593429976334002 4 1.6508010260812123 4.223834523245678 5 0.8301640660088552 2.655131953038978 6 0.4913104531142682 1.885642806772854 7 0.32150252348237635 1.4395760082203592 8 0.23067006304322768 1.1804114007296602 9 0.15974709634405201 0.9196607599572683 >10 0.8278965552191587 9.383946940538678 >50 0.04510863752284512 1.9428283852256942 >100 0.01627565045741363 1.5728135853127947 >500 1.6480704046016267E-4 0.07186211453658525 >1k 1.0987136017840657E-4 0.10482380805507815 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 3.5158875928545914E-5 0.0 0.0 0.0 4 0.0 3.5158875928545914E-5 0.0 0.0 0.0 5 0.0 3.5158875928545914E-5 0.0 0.0 0.0 6 0.0 3.5158875928545914E-5 0.0 0.0 0.0 7 0.0 3.5158875928545914E-5 0.0 0.0 0.0 8 0.0 3.5158875928545914E-5 0.0 0.0 0.0 9 0.0 3.5158875928545914E-5 0.0 0.0 0.0 10-11 0.0 3.5158875928545914E-5 0.0 0.0 0.0 12-13 0.0 5.273831389281887E-5 0.0 0.0 0.0 14-15 0.0 7.031775185709183E-5 0.0 0.0 0.0 16-17 0.0 7.031775185709183E-5 0.0 0.0 0.0 18-19 0.0 7.031775185709183E-5 0.0 3.5158875928545914E-5 0.0 20-21 0.0 7.031775185709183E-5 0.0 3.5158875928545914E-5 0.0 22-23 0.0 7.031775185709183E-5 0.0 5.273831389281887E-5 0.0 24-25 0.0 7.031775185709183E-5 0.0 7.031775185709183E-5 0.0 26-27 0.0 7.031775185709183E-5 0.0 4.2190651114255097E-4 0.0 28-29 1.7579437964272957E-5 7.031775185709183E-5 0.0 0.0013360372852847446 0.0 30-31 3.5158875928545914E-5 7.031775185709183E-5 0.0 0.004658551060532333 0.0 32-33 3.5158875928545914E-5 7.031775185709183E-5 0.0 0.0131318401593119 0.0 34-35 3.5158875928545914E-5 7.031775185709183E-5 0.0 0.024716689777767777 0.0 36-37 3.5158875928545914E-5 7.031775185709183E-5 0.0 0.04468693130518185 0.0 38-39 7.031775185709183E-5 7.031775185709183E-5 0.0 0.07986338667169204 0.0 40-41 7.031775185709183E-5 7.031775185709183E-5 0.0 0.12244078542116114 0.0 42-43 7.031775185709183E-5 7.031775185709183E-5 0.0 0.1666706513392719 0.0 44-45 7.031775185709183E-5 7.031775185709183E-5 0.0 0.21988361005712614 0.0 46-47 7.031775185709183E-5 7.031775185709183E-5 0.0 0.2838551848091154 0.0 48-49 7.031775185709183E-5 7.031775185709183E-5 0.0 0.3576185065072047 0.0 50-51 7.031775185709183E-5 7.031775185709183E-5 0.0 0.44018912662539483 0.0 52-53 7.031775185709183E-5 1.0547662778563774E-4 0.0 0.5219686720351926 0.0 54-55 7.031775185709183E-5 1.0547662778563774E-4 0.0 0.6104811421853069 0.0 56-57 7.031775185709183E-5 1.0547662778563774E-4 0.0 0.718805638921157 0.0 58-59 7.031775185709183E-5 1.0547662778563774E-4 0.0 0.8433735363359951 0.0 60-61 7.031775185709183E-5 1.0547662778563774E-4 0.0 0.9791746946100037 0.0 62-63 7.031775185709183E-5 1.0547662778563774E-4 0.0 1.1163646284831898 0.0 64-65 7.031775185709183E-5 1.230560657499107E-4 0.0 1.2583361694826583 0.0 66-67 7.031775185709183E-5 1.4063550371418366E-4 0.0 1.419979101564148 0.0 68-69 7.031775185709183E-5 1.4063550371418366E-4 0.0 1.6074813868910836 0.0 70-71 7.031775185709183E-5 1.4063550371418366E-4 0.0 1.8090296431514727 0.0 72-73 7.031775185709183E-5 1.4063550371418366E-4 0.0 2.0239734311406385 0.0 74-75 7.031775185709183E-5 1.4063550371418366E-4 0.0 2.245368872862692 0.0 76-77 7.031775185709183E-5 1.4063550371418366E-4 0.0 2.473497239325062 0.0 78-79 7.031775185709183E-5 1.4063550371418366E-4 0.0 2.7314754914507677 0.0 80-81 7.031775185709183E-5 1.4063550371418366E-4 0.0 3.0244544045633406 0.0 82-83 7.031775185709183E-5 1.4063550371418366E-4 0.0 3.324447513423659 0.0 84-85 7.031775185709183E-5 1.4063550371418366E-4 0.0 3.6198699684132656 0.0 86-87 7.031775185709183E-5 1.4063550371418366E-4 0.0 3.9300943101687906 0.0 88 7.031775185709183E-5 1.4063550371418366E-4 0.0 4.187070534330533 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4155 0.0 37.442146 1 TCAACGC 4105 0.0 35.495033 4 ATCAACG 4295 0.0 34.033062 3 CAACGCA 4335 0.0 33.826855 5 GGTATCA 2510 0.0 32.956627 1 AACGCAG 4555 0.0 32.089886 6 TATCAAC 5135 0.0 29.198063 2 GTACATG 9225 0.0 27.56352 1 ACGCAGA 5575 0.0 26.050121 7 TACATGG 9290 0.0 25.852884 2 CGCAGAG 5830 0.0 24.507622 8 ACATGGG 9520 0.0 24.339617 3 TACCGTC 220 6.315531E-8 21.363552 7 CATGGGG 6300 0.0 20.36766 4 GCAGAGT 6875 0.0 20.167194 9 GAGTACA 6630 0.0 19.424095 1 TATACCG 220 1.4898324E-6 19.227196 5 CATGGGA 3240 0.0 18.278671 4 GTATACG 235 2.798759E-6 18.000246 1 AGTACAT 6790 0.0 17.166656 2 >>END_MODULE