FastQCFastQC Report
Wed 25 May 2016
SRR1032961_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032961_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2844232
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68880.24217433739582425No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55000.19337381760700253No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29060.10217169344835442No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45850.052.568021
GTATCAA87900.043.7228051
ATCAACG103800.036.577653
TCAACGC104150.036.49994
CAACGCA106500.035.6459355
TATCAAC108700.035.0580252
AACGCAG109700.034.6061256
ACGCAGA125450.029.9223257
CGCAGAG127550.029.2821648
GCAGAGT141100.026.2012839
GTACATG96150.024.9209421
TACATGG94500.024.2453862
ACATGGG96750.022.466253
GTGGTAT21450.021.9036391
GAGTACT95700.021.7449812-13
CAGAGTA138850.021.28001810-11
AGAGTAC131350.020.88574410-11
GAGTACA70600.020.8297081
AGTACAT71550.019.6604522
GTACTTT106650.019.55641714-15
Produced by FastQC (version 0.11.5)