##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032961_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2844232 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.80189555563681 34.0 31.0 34.0 31.0 34.0 2 32.843013861035246 34.0 31.0 34.0 31.0 34.0 3 32.92887535194035 34.0 31.0 34.0 31.0 34.0 4 36.3556928548726 37.0 37.0 37.0 35.0 37.0 5 36.33759130760079 37.0 37.0 37.0 35.0 37.0 6 36.3948883213465 37.0 37.0 37.0 35.0 37.0 7 36.359099749950076 37.0 37.0 37.0 35.0 37.0 8 36.29239457259464 37.0 37.0 37.0 35.0 37.0 9 38.12809362949295 39.0 39.0 39.0 37.0 39.0 10-11 38.14115849199362 39.0 39.0 39.0 37.0 39.0 12-13 38.135099211316096 39.0 39.0 39.0 37.0 39.0 14-15 39.664495020096815 41.0 40.0 41.0 37.0 41.0 16-17 39.679003681837486 41.0 40.0 41.0 37.0 41.0 18-19 39.71413302431026 41.0 40.0 41.0 37.0 41.0 20-21 39.731248365112265 41.0 40.0 41.0 37.0 41.0 22-23 39.694112329795885 41.0 40.0 41.0 37.0 41.0 24-25 39.67638469716957 41.0 40.0 41.0 37.0 41.0 26-27 39.59880874696579 41.0 40.0 41.0 37.0 41.0 28-29 39.536381701633346 41.0 40.0 41.0 37.0 41.0 30-31 39.41883889921779 41.0 39.0 41.0 36.0 41.0 32-33 39.28601024810916 41.0 39.0 41.0 35.5 41.0 34-35 39.19465518284022 41.0 39.0 41.0 35.0 41.0 36-37 39.10129113940072 41.0 39.0 41.0 35.0 41.0 38-39 39.00160640904117 41.0 39.0 41.0 35.0 41.0 40-41 38.89496145180843 41.0 39.0 41.0 35.0 41.0 42-43 38.754338077906446 41.0 39.0 41.0 35.0 41.0 44-45 38.57327672285524 41.0 39.0 41.0 35.0 41.0 46-47 38.33774442450546 41.0 38.0 41.0 34.5 41.0 48-49 38.12285091370887 41.0 38.0 41.0 34.0 41.0 50-51 37.9682060042922 40.0 38.0 41.0 34.0 41.0 52-53 37.82624044030163 40.0 38.0 41.0 34.0 41.0 54-55 37.68138551988727 40.0 37.5 41.0 33.0 41.0 56-57 37.47734537829544 40.0 37.0 41.0 33.0 41.0 58-59 37.31177660612777 40.0 36.5 41.0 33.0 41.0 60-61 37.126650181841704 40.0 36.0 41.0 33.0 41.0 62-63 36.895035988625395 39.0 35.5 41.0 32.5 41.0 64-65 36.61435002489249 39.0 35.0 41.0 32.0 41.0 66-67 36.34392623386559 39.0 35.0 41.0 32.0 41.0 68-69 36.05620374849872 38.0 35.0 40.5 31.0 41.0 70-71 35.73724646934568 37.0 35.0 40.0 31.0 41.0 72-73 35.39954968511711 37.0 35.0 39.5 31.0 41.0 74-75 35.05807402490373 36.0 35.0 39.0 31.0 41.0 76-77 34.706275542923365 36.0 35.0 39.0 31.0 40.5 78-79 34.35755662688557 35.0 35.0 37.5 30.0 39.5 80-81 34.026002801459235 35.0 34.0 37.0 30.0 39.0 82-83 33.71484340939839 35.0 34.0 37.0 30.0 39.0 84-85 33.430023289239415 35.0 34.0 36.0 30.0 37.5 86-87 33.27949021036259 35.0 34.0 36.0 30.0 37.0 88-89 33.087127034644155 35.0 34.0 36.0 30.0 37.0 90-91 32.909479079062464 35.0 34.0 35.0 29.5 36.5 92-93 32.74513752745908 35.0 34.0 35.0 29.0 36.0 94-95 32.64159745055959 35.0 34.0 35.0 29.0 36.0 96-97 32.578364563790856 35.0 34.0 35.0 29.0 36.0 98-99 32.46617786453426 35.0 34.0 35.0 29.0 36.0 100 32.38203282995199 35.0 34.0 35.0 29.0 36.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16045845398856073 1101 2 0.20962416136455886 1101 3 0.1463338724969887 1101 4 0.08185919922285478 1101 5 0.05885341132275812 1101 6 0.0739366826201504 1101 7 0.10696026362983702 1101 8 0.04539695682866807 1101 9 0.11686997509659136 1101 10-11 0.1804963325319875 1101 12-13 0.19369481370048192 1101 14-15 0.260364937552481 1101 16-17 0.27560039276346515 1101 18-19 0.25350798843166444 1101 20-21 0.2604278771280235 1101 22-23 0.2603403023676236 1101 24-25 0.2521040245399675 1101 26-27 0.2827406380777333 1101 28-29 0.22500274054444702 1101 30-31 0.17053206984187597 1101 32-33 0.27194448794023884 1101 34-35 0.2502760648948126 1101 36-37 0.3101482513513645 1101 38-39 0.2457013295729169 1101 40-41 0.27918586639876963 1101 42-43 0.27000004429519464 1101 44-45 0.25667332622730044 1101 46-47 0.23041025200551246 1101 48-49 0.1950120650808458 1101 50-51 0.24504437113336763 1101 52-53 0.27232556101242267 1101 54-55 0.2268049851019569 1101 56-57 0.2962335204597153 1101 58-59 0.20905379460150186 1101 60-61 0.19773657122996724 1101 62-63 0.1459574473409191 1101 64-65 0.19688341702462253 1101 66-67 0.2699576738173377 1101 68-69 0.21317064769228722 1101 70-71 0.22415572361668268 1101 72-73 0.2489640445255077 1101 74-75 0.25819899702750604 1101 76-77 0.2112027834639747 1101 78-79 0.2392030001873522 1101 80-81 0.27710016765621504 1101 82-83 0.2174416408297617 1101 84-85 0.20599142317323071 1101 86-87 0.17371358646039425 1101 88-89 0.2173180030559152 1101 90-91 0.1975785447710834 1101 92-93 0.27617684765071004 1101 94-95 0.24281762409172813 1101 96-97 0.2202043323320808 1101 98-99 0.22458023992069798 1101 100 0.20239777382667512 1102 1 0.18607310614505224 1102 2 0.22526495119560508 1102 3 0.19207184499865093 1102 4 0.13275895908969204 1102 5 0.12072682645732868 1102 6 0.11177849590787758 1102 7 0.08945649222155083 1102 8 -0.00863394586227173 1102 9 0.11937739018276261 1102 10-11 0.16016010576883843 1102 12-13 0.14588693636470396 1102 14-15 0.2313942026051734 1102 16-17 0.22362594960080884 1102 18-19 0.2396523318570445 1102 20-21 0.22483949709914697 1102 22-23 0.22777953630787806 1102 24-25 0.19787184562058968 1102 26-27 0.21720566280364295 1102 28-29 0.19661203609109634 1102 30-31 0.25402506618207354 1102 32-33 0.20385181213613635 1102 34-35 0.1826871847005478 1102 36-37 0.20639929672389457 1102 38-39 0.21571944039988722 1102 40-41 0.16957482565143067 1102 42-43 0.16696322029155652 1102 44-45 0.23798455278877384 1102 46-47 0.22496127290758494 1102 48-49 0.22905732735841866 1102 50-51 0.18727782466773135 1102 52-53 0.22775322930060327 1102 54-55 0.20311744863156633 1102 56-57 0.2256869418866927 1102 58-59 0.1584643523934659 1102 60-61 0.19170278855452239 1102 62-63 0.17131489079668683 1102 64-65 0.1772199933657248 1102 66-67 0.16264645263399302 1102 68-69 0.19556980117582867 1102 70-71 0.20825590996862076 1102 72-73 0.2205389873157273 1102 74-75 0.2373236807266892 1102 76-77 0.1886888877403834 1102 78-79 0.23002597391253232 1102 80-81 0.2714693407967985 1102 82-83 0.14431188287215235 1102 84-85 0.17092613276806645 1102 86-87 0.19043147974231545 1102 88-89 0.18222514977377813 1102 90-91 0.2058907017477125 1102 92-93 0.21589253321341317 1102 94-95 0.19313089971780784 1102 96-97 0.21293909409470757 1102 98-99 0.22719064604960693 1102 100 0.24615095028018175 1103 1 0.2034581988834816 1103 2 0.20793075272456463 1103 3 0.18240044302561387 1103 4 0.11581771622963544 1103 5 0.13073276909242537 1103 6 0.14565689711989904 1103 7 0.14558852530684163 1103 8 0.06120349201268027 1103 9 0.10610183124376249 1103 10-11 0.13251777589800184 1103 12-13 0.1756571004465215 1103 14-15 0.25264781074940146 1103 16-17 0.2439223565174018 1103 18-19 0.222201044154275 1103 20-21 0.20170348181297015 1103 22-23 0.20598732418108057 1103 24-25 0.1954847748132451 1103 26-27 0.2213609690269962 1103 28-29 0.21334847004582969 1103 30-31 0.22822860979754722 1103 32-33 0.2987512729421766 1103 34-35 0.2713192596034304 1103 36-37 0.16459983491942154 1103 38-39 0.1681626375349694 1103 40-41 0.143791854626933 1103 42-43 0.14221774673693233 1103 44-45 0.1816644702479948 1103 46-47 0.1595068363732537 1103 48-49 0.09439485619472521 1103 50-51 0.12200025381675772 1103 52-53 0.10849982421477478 1103 54-55 0.19534372208069328 1103 56-57 0.2241664027108783 1103 58-59 0.27411266042813054 1103 60-61 0.23455203376984457 1103 62-63 0.24563916080108328 1103 64-65 0.21648404540262334 1103 66-67 0.25687861503610776 1103 68-69 0.3054237806601918 1103 70-71 0.295331592791392 1103 72-73 0.2702172382956505 1103 74-75 0.1910879893095938 1103 76-77 0.2737148282567148 1103 78-79 0.2540259599085033 1103 80-81 0.19752830138415334 1103 82-83 0.20029068207086453 1103 84-85 0.22350256915017042 1103 86-87 0.1972949444917802 1103 88-89 0.24390024932880294 1103 90-91 0.18355609288085617 1103 92-93 0.21324282205296186 1103 94-95 0.19668814967428006 1103 96-97 0.21201806993866512 1103 98-99 0.1953416294370669 1103 100 0.2230684225998587 1104 1 0.09661380213103143 1104 2 0.13070970362151257 1104 3 0.0877583727495761 1104 4 0.09459477239466452 1104 5 0.08051078210699103 1104 6 0.09533028086578099 1104 7 0.10199878187603417 1104 8 0.04200191374710727 1104 9 0.051373595541789996 1104 10-11 0.10894941328469088 1104 12-13 0.13623514120519786 1104 14-15 0.20414783681374615 1104 16-17 0.14945728202141595 1104 18-19 0.16587637531861787 1104 20-21 0.12993759699181595 1104 22-23 0.15600695146659405 1104 24-25 0.14770170971741692 1104 26-27 0.15929129795897978 1104 28-29 0.1693262321113096 1104 30-31 0.20842928724726306 1104 32-33 0.22084768521818887 1104 34-35 0.2180156679248526 1104 36-37 0.16905063880134463 1104 38-39 0.13312537606474706 1104 40-41 0.20504460540664837 1104 42-43 0.2785865024290146 1104 44-45 0.264132323865951 1104 46-47 0.28890277652209306 1104 48-49 0.3037730539964798 1104 50-51 0.21963126246087938 1104 52-53 0.23884582663599474 1104 54-55 0.22253648471053822 1104 56-57 0.2597001883261214 1104 58-59 0.30595491717732415 1104 60-61 0.26703480387683953 1104 62-63 0.16428578245030678 1104 64-65 0.20393197328131407 1104 66-67 0.1594140028800055 1104 68-69 0.21417084950656573 1104 70-71 0.16568328411918287 1104 72-73 0.14062914723869113 1104 74-75 0.16604802966872256 1104 76-77 0.13704008773947862 1104 78-79 0.20440382466935603 1104 80-81 0.16062984142409675 1104 82-83 0.19619718590183055 1104 84-85 0.15569914597553236 1104 86-87 0.14525713142448637 1104 88-89 0.187798134633681 1104 90-91 0.1749675051447852 1104 92-93 0.19830237612773516 1104 94-95 0.19136154054833554 1104 96-97 0.12029762322870852 1104 98-99 0.09187450183962653 1104 100 0.13484295297219262 1105 1 0.10906125285046642 1105 2 0.14053262560918256 1105 3 0.10741126741225315 1105 4 0.09765793753066987 1105 5 0.08844316386442586 1105 6 0.0909227647613875 1105 7 0.11248160039665578 1105 8 0.006413509689899399 1105 9 0.10783496192794928 1105 10-11 0.096188326482185 1105 12-13 0.1419121096629965 1105 14-15 0.17331416533622246 1105 16-17 0.16833495958715616 1105 18-19 0.16299578115838642 1105 20-21 0.16617875726726794 1105 22-23 0.17640859327291025 1105 24-25 0.19378592826553387 1105 26-27 0.184173771067627 1105 28-29 0.125277899530154 1105 30-31 0.24336717577076428 1105 32-33 0.1783392107999049 1105 34-35 0.16216944413256584 1105 36-37 0.1480615791377673 1105 38-39 0.1747279267883286 1105 40-41 0.16226046703917518 1105 42-43 0.1855277965539912 1105 44-45 0.08871074504616416 1105 46-47 0.15574851471322404 1105 48-49 0.1732548828515661 1105 50-51 0.11404811594586306 1105 52-53 0.035882858937171136 1105 54-55 0.00263934945858324 1105 56-57 0.07212191001195833 1105 58-59 0.09727662050148922 1105 60-61 0.05544768041565362 1105 62-63 0.10515020388733376 1105 64-65 0.03443497349815061 1105 66-67 0.0848649645429731 1105 68-69 0.14291980004461635 1105 70-71 0.09823697674637799 1105 72-73 0.14974514819399332 1105 74-75 0.16355220585655417 1105 76-77 0.09055834187934408 1105 78-79 0.09530985412437332 1105 80-81 0.13289548479303903 1105 82-83 0.12241377453192825 1105 84-85 0.09544704924751812 1105 86-87 0.06750884371079025 1105 88-89 0.06314159422789345 1105 90-91 0.060852369691218655 1105 92-93 0.08762101503197783 1105 94-95 0.06694051102488885 1105 96-97 0.0392582479482968 1105 98-99 0.013462134981850227 1105 100 -0.03741089543336784 1106 1 0.0915440231673017 1106 2 0.1138828586519054 1106 3 0.06186862891613032 1106 4 0.05055507854834218 1106 5 0.04747514740839165 1106 6 0.045670112785572314 1106 7 0.04088811384641389 1106 8 0.07210180053254334 1106 9 0.0810317908957785 1106 10-11 0.03994558152754024 1106 12-13 0.0670182538578814 1106 14-15 0.08863669482013847 1106 16-17 0.055726780864972625 1106 18-19 0.12551153564780293 1106 20-21 0.11751903638345595 1106 22-23 0.15335851149280444 1106 24-25 0.14346046418233271 1106 26-27 0.1111679565824133 1106 28-29 0.14364470761906745 1106 30-31 0.11416520592924684 1106 32-33 0.07610196852750306 1106 34-35 0.137844937332126 1106 36-37 0.11461883057766897 1106 38-39 0.16989931536675584 1106 40-41 0.2076801583697403 1106 42-43 0.12568235230767044 1106 44-45 0.10488350742927821 1106 46-47 0.04871429486853174 1106 48-49 0.016004309868399957 1106 50-51 0.09282180308227339 1106 52-53 0.04058875283999441 1106 54-55 0.05808131595643573 1106 56-57 0.023821586399392913 1106 58-59 0.00940820743966242 1106 60-61 0.08716856583504295 1106 62-63 0.14365052174723303 1106 64-65 0.12722437341436432 1106 66-67 0.09989753047127437 1106 68-69 0.15062786680822882 1106 70-71 0.16804415551741414 1106 72-73 0.10592474601467217 1106 74-75 0.11944121433402444 1106 76-77 0.04689880729651463 1106 78-79 0.008141978682971285 1106 80-81 0.04855258098594106 1106 82-83 0.036612756955392456 1106 84-85 0.044662779526412066 1106 86-87 0.056716591466269506 1106 88-89 -0.004532854537934838 1106 90-91 0.05798990821376293 1106 92-93 0.016157135405030942 1106 94-95 0.00830711398179318 1106 96-97 -0.017244457205116248 1106 98-99 -0.029528639359085673 1106 100 -0.00213456055526251 1107 1 0.07939412128810375 1107 2 0.07575767880697981 1107 3 0.0657317303110787 1107 4 0.06013984091972446 1107 5 0.06832160456058034 1107 6 0.05196824965695157 1107 7 0.03727809152619699 1107 8 0.04171789156583117 1107 9 0.08975200144720219 1107 10-11 0.056907955376857444 1107 12-13 0.06975967379675296 1107 14-15 0.10015803058510642 1107 16-17 0.0677562190488743 1107 18-19 0.1058996085915993 1107 20-21 0.12104974871193264 1107 22-23 0.10875008412163822 1107 24-25 0.10958813074789475 1107 26-27 0.10916292870158628 1107 28-29 0.15318353662446071 1107 30-31 0.16445503169678233 1107 32-33 0.16247581525166765 1107 34-35 0.15407667092054567 1107 36-37 0.15223647508225469 1107 38-39 0.11629361904609681 1107 40-41 0.1234886731034166 1107 42-43 0.14456841876539528 1107 44-45 0.152994860277893 1107 46-47 0.1747671167812257 1107 48-49 0.2136939830755864 1107 50-51 0.20187498598100007 1107 52-53 0.16666636418830905 1107 54-55 0.18595470318492602 1107 56-57 0.20400505738700758 1107 58-59 0.1539311031568218 1107 60-61 0.15826782662379912 1107 62-63 0.1269944513491268 1107 64-65 0.11738628893456848 1107 66-67 0.09789023427607191 1107 68-69 0.0639507140482678 1107 70-71 0.14371875221112163 1107 72-73 0.08656808533635285 1107 74-75 0.08966343137153387 1107 76-77 0.09837771586141741 1107 78-79 0.08064516404444078 1107 80-81 0.14001693021303652 1107 82-83 0.1381937072624595 1107 84-85 0.1517602703818639 1107 86-87 0.14794730431322733 1107 88-89 0.18170871705395797 1107 90-91 0.12248205530941192 1107 92-93 0.16144680239056441 1107 94-95 0.16060392810185675 1107 96-97 0.15214677906510587 1107 98-99 0.11946186353269184 1107 100 0.1616327142788947 1108 1 -0.02374389366497809 1108 2 0.0076709709942406334 1108 3 0.013642438324524164 1108 4 -0.02252173266650459 1108 5 -0.04354803265152185 1108 6 0.01714713469885254 1108 7 0.03571065211629332 1108 8 0.04489730347812326 1108 9 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-0.22678985838348353 2214 36-37 -0.14315042967965041 2214 38-39 -0.1852459871810126 2214 40-41 -0.2030623800101381 2214 42-43 -0.18594611878038592 2214 44-45 -0.1605353595023331 2214 46-47 -0.1712293346839715 2214 48-49 -0.1874764610636106 2214 50-51 -0.15669873196772244 2214 52-53 -0.17745565962121645 2214 54-55 -0.2394259057894388 2214 56-57 -0.23160620634925522 2214 58-59 -0.2308702933633242 2214 60-61 -0.18566818528097428 2214 62-63 -0.1831732455373185 2214 64-65 -0.20209631179803722 2214 66-67 -0.1974537446616509 2214 68-69 -0.3001890524352433 2214 70-71 -0.22757531830169597 2214 72-73 -0.19057534415082955 2214 74-75 -0.16533027284092583 2214 76-77 -0.170137622966422 2214 78-79 -0.15805103558925993 2214 80-81 -0.17764847729611688 2214 82-83 -0.1721743101368105 2214 84-85 -0.1777383559917638 2214 86-87 -0.16681345576003537 2214 88-89 -0.20312129668230483 2214 90-91 -0.14855159853674849 2214 92-93 -0.1948947727010122 2214 94-95 -0.20068842537799725 2214 96-97 -0.16470303778471873 2214 98-99 -0.16935739013031537 2214 100 -0.1655765348053535 2215 1 -0.18378621463613598 2215 2 -0.20283376227227023 2215 3 -0.216908625268303 2215 4 -0.12152964000585342 2215 5 -0.16186833558057856 2215 6 -0.1525191978548861 2215 7 -0.16270721178837277 2215 8 -0.13682115069271816 2215 9 -0.24044352254100687 2215 10-11 -0.20445948409424375 2215 12-13 -0.25396729841801857 2215 14-15 -0.3267233014974451 2215 16-17 -0.2857192343595045 2215 18-19 -0.2680632029870651 2215 20-21 -0.24605967235479653 2215 22-23 -0.27459725350009023 2215 24-25 -0.32073852430436744 2215 26-27 -0.30415222362575633 2215 28-29 -0.2609410725062489 2215 30-31 -0.33488294739729696 2215 32-33 -0.326762190644871 2215 34-35 -0.2786926491902335 2215 36-37 -0.25794522330065917 2215 38-39 -0.27913470669320617 2215 40-41 -0.226400521035778 2215 42-43 -0.1906595138710756 2215 44-45 -0.23269827382377883 2215 46-47 -0.16376750307046706 2215 48-49 -0.15177557379031015 2215 50-51 -0.11293724041049558 2215 52-53 -0.18814347231665352 2215 54-55 -0.18154107542671483 2215 56-57 -0.18917038708721634 2215 58-59 -0.12327540984237118 2215 60-61 -0.14723559069059888 2215 62-63 -0.16219374663175046 2215 64-65 -0.18808568015325733 2215 66-67 -0.14302904563101038 2215 68-69 -0.14585425334205837 2215 70-71 -0.1793356567945068 2215 72-73 -0.29353103911643075 2215 74-75 -0.23829136180278 2215 76-77 -0.2138062830664893 2215 78-79 -0.19447430794698306 2215 80-81 -0.16588731797779843 2215 82-83 -0.16965208921748598 2215 84-85 -0.1378391220955777 2215 86-87 -0.2742552553222666 2215 88-89 -0.2894421808286509 2215 90-91 -0.2960018408757108 2215 92-93 -0.25379940059345074 2215 94-95 -0.3167078515818744 2215 96-97 -0.2680726437884715 2215 98-99 -0.32025898878572434 2215 100 -0.3313261439360602 2216 1 -0.18325887295614507 2216 2 -0.21044259676605037 2216 3 -0.2175947777107865 2216 4 -0.13310855924110854 2216 5 -0.1641684005536419 2216 6 -0.18423597058278318 2216 7 -0.15222503790938902 2216 8 -0.1016886931901766 2216 9 -0.18313056893914847 2216 10-11 -0.2485514417189023 2216 12-13 -0.22835263017233842 2216 14-15 -0.264419550109281 2216 16-17 -0.2747433389062337 2216 18-19 -0.28856219613493295 2216 20-21 -0.27840984492511467 2216 22-23 -0.30872097880686056 2216 24-25 -0.2973028775919673 2216 26-27 -0.350756878646429 2216 28-29 -0.3026987183615617 2216 30-31 -0.36258202533272055 2216 32-33 -0.40245002501424665 2216 34-35 -0.42147675889795266 2216 36-37 -0.4236490290634478 2216 38-39 -0.37731422589251906 2216 40-41 -0.2903687810017743 2216 42-43 -0.3714514479948363 2216 44-45 -0.3687836143333314 2216 46-47 -0.30506646672701976 2216 48-49 -0.32298231255711585 2216 50-51 -0.2958304680051569 2216 52-53 -0.2708593280488003 2216 54-55 -0.25154847590502527 2216 56-57 -0.3014820734838821 2216 58-59 -0.3209324165654692 2216 60-61 -0.4344368366989073 2216 62-63 -0.3223604831867064 2216 64-65 -0.4752515918728335 2216 66-67 -0.4622290382581795 2216 68-69 -0.44312330174007997 2216 70-71 -0.4313368364473362 2216 72-73 -0.3743469718024883 2216 74-75 -0.44282487822805905 2216 76-77 -0.45655345489490173 2216 78-79 -0.4342378428504219 2216 80-81 -0.376755883455445 2216 82-83 -0.3921835136021983 2216 84-85 -0.4253961993213693 2216 86-87 -0.32719396406321266 2216 88-89 -0.33245263642347567 2216 90-91 -0.3673047397803799 2216 92-93 -0.3679247663549461 2216 94-95 -0.3876113827067442 2216 96-97 -0.38997153463832035 2216 98-99 -0.3102479387564614 2216 100 -0.3395209709740712 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14.0 3 2.0 4 8.0 5 31.0 6 66.0 7 157.0 8 339.0 9 690.0 10 1138.0 11 1918.0 12 3032.0 13 4774.0 14 5976.0 15 7559.0 16 9843.0 17 14441.0 18 17077.0 19 9440.0 20 6166.0 21 5715.0 22 5821.0 23 6047.0 24 6737.0 25 8069.0 26 10103.0 27 13097.0 28 16462.0 29 21245.0 30 26828.0 31 34386.0 32 44106.0 33 58014.0 34 80822.0 35 124760.0 36 237697.0 37 543236.0 38 993769.0 39 522763.0 40 1884.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.724698766163925 20.25839079198894 13.912958179380702 23.103952262466425 2 15.43437086551413 20.631266882941773 40.30743786426985 23.626924387274247 3 18.01995619200979 26.322378991853686 30.35833951544003 25.299325300696495 4 12.260003699148141 17.328856379518534 39.28024141512811 31.130898506205217 5 12.46911718120159 38.091265124099095 35.675136127000066 13.764481567699253 6 29.707524740360704 38.76629755658833 18.005244471259836 13.520933231791144 7 26.575787397076102 33.15395155004214 22.90440272933145 17.36585832355031 8 25.236620493894446 35.855759555130234 20.50885817276377 18.39876177821155 9 25.26869641497858 16.619219146366547 21.56459033223812 36.54749410641676 10-11 23.65777475255183 27.039636710366803 28.90333840558717 20.399250131494195 12-13 24.770328674481913 24.409334344738596 29.224754348940092 21.595582631839402 14-15 21.814763411154697 26.101164551710436 26.914003890335774 25.170068146799085 16-17 20.933875998863666 29.688840432144776 27.35610175259965 22.02118181639191 18-19 20.76914341285459 28.101481983342406 30.248021520067475 20.881353083735522 20-21 21.828300223047908 27.196761726891477 30.157807098717687 20.81713095134293 22-23 21.46437704592092 27.010050008323883 30.262674227949145 21.262898717806053 24-25 21.204007992357827 27.228653791184005 30.114597444569103 21.452740771889065 26-27 21.058915258826126 27.25310891099553 30.1995311251502 21.488444705028144 28-29 21.159048391432435 27.301810773572694 30.19756818332454 21.34157265167033 30-31 21.30416245064952 27.558326088084062 29.896732121523268 21.240779339743142 32-33 21.129514587551686 27.3683188886693 30.196298799532727 21.305867724246287 34-35 21.303462894792602 27.22118232707726 30.084675501428254 21.390679276701885 36-37 21.538621705758445 27.468634603284137 29.650046901692157 21.342696789265265 38-39 21.33161614919875 27.607867883046467 29.759729640226706 21.300786327528076 40-41 21.25484752459644 27.354954184208747 29.896246088209473 21.493952202985344 42-43 21.297701530583073 27.43788960556326 29.619988618042076 21.644420245811588 44-45 21.41449121922143 27.57167462626101 29.46467775654007 21.549156397977487 46-47 21.543193993253638 27.71324903491416 29.132216496024732 21.61134047580747 48-49 21.583405240648542 27.736113675213826 28.86770573397115 21.81277535016648 50-51 21.609193015234514 27.78510114280867 28.714427365457663 21.891278476499153 52-53 21.693532704293492 27.7568655125959 28.680503431326393 21.86909835178421 54-55 21.688123597837112 28.093487909829324 28.53109209030092 21.68729640203265 56-57 21.586732294992427 27.81163952565164 28.61471372423152 21.986914455124413 58-59 21.50637249312708 27.46100067990152 28.939100249578757 22.09352657739264 60-61 21.699291155526996 27.77681780845926 28.595585611393314 21.928305424620433 62-63 21.436250798415664 27.74868778296467 28.765539412260498 22.049522006359172 64-65 21.579382968542287 27.56223035330112 28.79512950952296 22.06325716863363 66-67 21.405227178317652 27.67766184913635 28.691574047883954 22.225536924662048 68-69 21.742718938144197 27.703703781834648 28.65257285984105 21.901004420180097 70-71 21.73704536057537 27.548737233812147 28.60116544641928 22.113051959193204 72-73 21.493851415777616 27.670861589349954 28.689343907248073 22.145943087624357 74-75 21.61001634184553 27.76798798410256 28.499257444540387 22.122738229511516 76-77 21.673145574223177 27.73935788233685 28.3609673551308 22.226529188309172 78-79 21.647938995120477 27.784218689653073 28.4392110839079 22.12863123131855 80-81 21.63464564160712 27.676643022264415 28.469829215339853 22.218882120788617 82-83 21.52321798336526 27.677189086601167 28.622331152366975 22.177261777666597 84-85 21.4868723789058 27.651436310399436 28.43792278548304 22.423768525211727 86-87 21.600576470098073 27.72080719174223 28.381255017875812 22.297361320283883 88-89 21.291236488604344 27.723879960741428 28.436483189852257 22.54840036080197 90-91 21.297188779730174 27.556252093055143 28.5321058178363 22.614453309378383 92-93 21.39746681276498 27.653682305081194 28.398759090198716 22.55009179195511 94-95 21.190381051111586 27.47315280561466 28.474928877151157 22.8615372661226 96-97 21.295966843680386 27.420062554848002 28.479050876442923 22.80491972502869 98-99 21.17041850159472 27.495521196330607 28.40251519699265 22.931545105082023 100 21.188320783958552 27.346046314084084 28.39662868570496 23.069004216252402 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 56.0 1 61.5 2 213.0 3 378.0 4 244.5 5 153.5 6 428.5 7 999.0 8 1773.0 9 2118.5 10 1693.0 11 1321.5 12 1348.5 13 1534.5 14 1821.5 15 2056.5 16 2319.5 17 2550.0 18 2847.0 19 3346.0 20 4011.5 21 4963.5 22 6325.0 23 8505.0 24 11434.0 25 15007.5 26 19885.0 27 25989.0 28 33657.0 29 41867.0 30 49108.0 31 57035.0 32 65224.5 33 72098.5 34 78688.5 35 84765.5 36 90074.0 37 94880.0 38 97974.5 39 98993.5 40 99785.5 41 101036.5 42 101093.0 43 100685.0 44 99416.5 45 97811.5 46 96682.5 47 93364.0 48 90488.0 49 88179.0 50 85411.0 51 82155.5 52 75887.0 53 71360.0 54 69173.5 55 66009.5 56 60896.0 57 54294.0 58 48895.0 59 45534.5 60 41884.0 61 36477.0 62 31542.0 63 27173.0 64 22267.5 65 17365.5 66 13535.0 67 10418.0 68 7620.5 69 5670.0 70 4099.5 71 2942.0 72 2287.5 73 1635.5 74 1226.5 75 881.0 76 542.0 77 349.5 78 207.5 79 113.0 80 55.5 81 25.5 82 14.5 83 9.0 84 4.5 85 3.0 86 3.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.570653870710969E-4 2 5.273831389281887E-4 3 7.031775185709183E-5 4 0.011743064560134334 5 4.570653870710969E-4 6 0.003304934337283316 7 6.680186426423724E-4 8 0.14169026999204004 9 0.0013008784093561988 10-11 0.0 12-13 1.5821494167845662E-4 14-15 1.5821494167845662E-4 16-17 0.0 18-19 1.0547662778563774E-4 20-21 0.0 22-23 1.9337381760700252E-4 24-25 5.273831389281887E-4 26-27 0.006662606988459451 28-29 0.00796348539781565 30-31 0.014573354072382281 32-33 0.017790391219844232 34-35 0.02564839998987424 36-37 0.036951978600901755 38-39 0.042384024931862094 40-41 0.054285304433674894 42-43 0.07082755555805574 44-45 0.10939684245167061 46-47 0.11577817843270169 48-49 0.11217439365002575 50-51 0.11423118789184566 52-53 0.11002970221838443 54-55 0.11632314100959416 56-57 0.09760103957764346 58-59 0.09296006795507539 60-61 0.09513991826264524 62-63 0.09376872210143194 64-65 3.5158875928545914E-5 66-67 5.273831389281887E-5 68-69 1.4063550371418366E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 8.789718982136479E-5 78-79 1.7579437964272957E-5 80-81 2.461121314998214E-4 82-83 1.230560657499107E-4 84-85 0.0 86-87 8.965513361779208E-4 88-89 4.3948594910682393E-4 90-91 1.5821494167845662E-4 92-93 0.0010020279639635585 94-95 0.0011778223436062882 96-97 2.812710074283673E-4 98-99 5.273831389281887E-5 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2844232.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.89804024820107 #Duplication Level Percentage of deduplicated Percentage of total 1 76.90658563148143 47.603669327691605 2 14.07368982082696 17.4226763794049 3 4.203465661299736 7.805588600551867 4 1.7346824416684534 4.294937743689665 5 0.8654501735690955 2.678483483819624 6 0.5106869367454419 1.8966312338939928 7 0.33280612765889545 1.4420032959274776 8 0.23756224743933432 1.1763710042762416 9 0.17438681749882992 0.9714782023468442 >10 0.8895370610392479 9.81998062103933 >50 0.05076702552690429 2.1304694254837573 >100 0.019583693239649435 1.9295137052499824 >500 5.119470040603882E-4 0.1949195844448829 >1k 1.7064900119435214E-4 0.19710257096758038 >5k 1.1376600079623476E-4 0.4361748212122875 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6888 0.24217433739582425 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5500 0.19337381760700253 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2906 0.10217169344835442 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 3.5158875928545914E-5 0.0 20-21 0.0 0.0 0.0 3.5158875928545914E-5 0.0 22-23 0.0 0.0 0.0 5.273831389281887E-5 0.0 24-25 0.0 0.0 0.0 7.031775185709183E-5 0.0 26-27 0.0 0.0 0.0 4.04327073178278E-4 0.0 28-29 0.0 0.0 0.0 0.0013008784093561988 0.0 30-31 0.0 0.0 0.0 0.004658551060532334 0.0 32-33 0.0 0.0 0.0 0.01320215791116899 0.0 34-35 0.0 0.0 0.0 0.024874904719446235 0.0 36-37 0.0 0.0 0.0 0.04496820231261022 0.0 38-39 0.0 0.0 0.0 0.0802149754309775 0.0 40-41 0.0 0.0 0.0 0.12303848631194643 0.0 42-43 0.0 0.0 0.0 0.1675847821134141 0.0 44-45 0.0 1.7579437964272957E-5 0.0 0.22097353521091107 0.0 46-47 0.0 3.5158875928545914E-5 0.0 0.2851209043425431 0.0 48-49 0.0 3.5158875928545914E-5 0.0 0.358989702668418 0.0 50-51 0.0 3.5158875928545914E-5 0.0 0.44175369660421515 0.0 52-53 0.0 5.273831389281887E-5 0.0 0.5240430457149768 0.0 54-55 0.0 8.789718982136479E-5 0.0 0.6131707961938407 0.0 56-57 0.0 1.0547662778563774E-4 0.0 0.7219875171926904 0.0 58-59 0.0 1.0547662778563774E-4 0.0 0.8461335080963859 0.0 60-61 0.0 1.0547662778563774E-4 0.0 0.9825147878232157 0.0 62-63 0.0 1.0547662778563774E-4 0.0 1.12010904876958 0.0 64-65 0.0 1.0547662778563774E-4 0.0 1.2632759915506189 0.0 66-67 0.0 1.0547662778563774E-4 0.0 1.42537598901918 0.0 68-69 0.0 1.0547662778563774E-4 0.0 1.6139681994999002 0.0 70-71 0.0 1.0547662778563774E-4 0.0 1.8171161846150383 0.0 72-73 0.0 1.0547662778563774E-4 0.0 2.034064028532131 0.0 74-75 0.0 1.0547662778563774E-4 0.0 2.2568658252913263 0.0 76-77 0.0 1.0547662778563774E-4 0.0 2.486453285104731 0.0 78-79 0.0 1.0547662778563774E-4 0.0 2.7463301165305785 0.0 80-81 0.0 1.0547662778563774E-4 0.0 3.0408560201840076 0.0 82-83 0.0 1.0547662778563774E-4 0.0 3.342360960709253 0.0 84-85 0.0 1.0547662778563774E-4 0.0 3.640402751955537 0.0 86-87 1.7579437964272957E-5 1.0547662778563774E-4 0.0 3.952121345938025 0.0 88 3.5158875928545914E-5 1.0547662778563774E-4 0.0 4.210626981202658 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4585 0.0 52.56802 1 GTATCAA 8790 0.0 43.722805 1 ATCAACG 10380 0.0 36.57765 3 TCAACGC 10415 0.0 36.4999 4 CAACGCA 10650 0.0 35.645935 5 TATCAAC 10870 0.0 35.058025 2 AACGCAG 10970 0.0 34.606125 6 ACGCAGA 12545 0.0 29.922325 7 CGCAGAG 12755 0.0 29.282164 8 GCAGAGT 14110 0.0 26.201283 9 GTACATG 9615 0.0 24.920942 1 TACATGG 9450 0.0 24.245386 2 ACATGGG 9675 0.0 22.46625 3 GTGGTAT 2145 0.0 21.903639 1 GAGTACT 9570 0.0 21.74498 12-13 CAGAGTA 13885 0.0 21.280018 10-11 AGAGTAC 13135 0.0 20.885744 10-11 GAGTACA 7060 0.0 20.829708 1 AGTACAT 7155 0.0 19.660452 2 GTACTTT 10665 0.0 19.556417 14-15 >>END_MODULE