FastQCFastQC Report
Wed 25 May 2016
SRR1032960_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032960_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3250833
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46750.034.584341
TCAACGC47550.032.6191644
ATCAACG49050.031.8132823
CAACGCA49950.030.9577795
GGTATCA29950.029.1889721
AACGCAG53000.029.0875646
GTACATG99900.027.9462571
TATCAAC58250.027.191722
TACATGG99200.026.6273142
ACATGGG102600.024.6458783
ACGCAGA63150.023.891377
CGCAGAG65650.022.62368
CATGGGG69200.020.3762654
TATACCG2201.4895322E-619.2277835
GTCTTAC11050.019.1404841
GAGTACA71000.019.0651
GCAGAGT76800.018.3598619
AGTACAT74250.017.0277982
AGAGTAC62150.016.59979810-11
CATGGGA33550.016.1107124
Produced by FastQC (version 0.11.5)