FastQCFastQC Report
Wed 25 May 2016
SRR1032960_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032960_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3250833
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69440.21360678939828653No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54200.16672649748541374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48900.051.7784961
GTATCAA89650.042.442821
TCAACGC107250.034.962774
ATCAACG107650.034.832863
CAACGCA108900.034.475175
TATCAAC113250.033.235072
AACGCAG113600.033.214486
ACGCAGA132500.028.2264257
TATACCG2700.027.879675
CGCAGAG135050.027.5881048
GTACATG99250.027.1202471
TACATGG101250.025.4642162
GCAGAGT150950.024.5815949
TATAACG2751.8189894E-1223.951912
ACATGGG103750.023.6715773
GAGTACT97400.021.43493512-13
CAGAGTA146400.020.78946510-11
AGAGTAC137950.020.6669510-11
GAGTACA74250.020.1819781
GTGGTAT23550.020.1465171
Produced by FastQC (version 0.11.5)