FastQCFastQC Report
Wed 25 May 2016
SRR1032959_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032959_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2740255
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA45050.035.7857741
GGTATCA26750.034.9655531
ATCAACG47050.031.8676553
TCAACGC47550.031.5325574
CAACGCA50150.030.1783875
AACGCAG51250.029.7129926
TATCAAC55200.028.3541262
GTACATG99900.028.276141
TACATGG99650.027.3565792
ACATGGG103800.025.2218463
ACGCAGA62850.024.0794097
CGCAGAG66150.022.6650188
CATGGGG67850.021.0592444
GCAGAGT74850.018.9631469
CATGGGA35600.017.9559334
GAGTACA74900.016.9431081
TATTACG3101.260978E-716.6779272
AGTACAT74200.015.962752
GTATAGG13000.015.9081761
GTATTAC10050.015.9009821
Produced by FastQC (version 0.11.5)