##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032959_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2740255 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.71703728302658 33.0 31.0 34.0 30.0 34.0 2 31.817192925475915 34.0 31.0 34.0 30.0 34.0 3 31.88633612565254 34.0 31.0 34.0 30.0 34.0 4 35.2027668957816 37.0 35.0 37.0 33.0 37.0 5 35.27900104187384 37.0 35.0 37.0 33.0 37.0 6 35.306621464060825 37.0 37.0 37.0 33.0 37.0 7 35.27123570616603 37.0 37.0 37.0 33.0 37.0 8 35.26759407427411 37.0 37.0 37.0 33.0 37.0 9 36.9151849006753 39.0 38.0 39.0 34.0 39.0 10-11 36.918421825706005 39.0 38.0 39.0 34.0 39.0 12-13 36.873792402531876 39.0 38.0 39.0 34.0 39.0 14-15 38.36414603750381 41.0 39.0 41.0 34.0 41.0 16-17 38.38508113296025 41.0 39.0 41.0 34.0 41.0 18-19 38.39711267746979 41.0 39.0 41.0 34.0 41.0 20-21 38.44342825758917 41.0 39.0 41.0 34.0 41.0 22-23 38.43449678953236 41.0 39.0 41.0 34.0 41.0 24-25 38.466870054064316 41.0 39.0 41.0 34.0 41.0 26-27 38.45135543954851 41.0 39.0 41.0 34.0 41.0 28-29 38.437266239820744 41.0 39.0 41.0 34.0 41.0 30-31 38.38843337572598 41.0 39.0 41.0 34.0 41.0 32-33 38.33911643259478 41.0 39.0 41.0 34.0 41.0 34-35 38.267066386157495 41.0 39.0 41.0 33.5 41.0 36-37 38.177262882468966 41.0 39.0 41.0 33.0 41.0 38-39 38.083428367068024 41.0 38.0 41.0 33.0 41.0 40-41 37.99899626129685 41.0 38.0 41.0 33.0 41.0 42-43 37.878947579696046 40.5 38.0 41.0 33.0 41.0 44-45 37.74155379700065 40.0 38.0 41.0 33.0 41.0 46-47 37.613258255162386 40.0 38.0 41.0 32.0 41.0 48-49 37.47704793896918 40.0 38.0 41.0 32.0 41.0 50-51 37.33308250509533 40.0 37.0 41.0 32.0 41.0 52-53 37.17328661018774 40.0 37.0 41.0 31.0 41.0 54-55 37.001008482787185 40.0 36.5 41.0 31.0 41.0 56-57 36.81366715871333 40.0 36.0 41.0 31.0 41.0 58-59 36.59634304106734 39.5 35.5 41.0 31.0 41.0 60-61 36.338973927608926 39.0 35.0 41.0 30.0 41.0 62-63 36.08190569855725 39.0 35.0 41.0 30.0 41.0 64-65 35.8010723454569 38.5 35.0 40.5 30.0 41.0 66-67 35.498357452134925 37.5 35.0 40.0 29.5 41.0 68-69 35.18135082318982 37.0 35.0 40.0 29.0 41.0 70-71 34.84959538437116 36.5 34.5 39.5 29.0 41.0 72-73 34.5580659464174 36.0 34.0 39.0 29.0 41.0 74-75 34.285567766503476 36.0 34.0 39.0 29.0 40.5 76-77 33.96418727454197 35.0 34.0 37.5 29.0 40.0 78-79 33.60480137797395 35.0 34.0 37.0 28.5 39.0 80-81 33.289962430503735 35.0 34.0 37.0 28.0 39.0 82-83 32.98131998664358 35.0 34.0 36.0 28.0 38.5 84-85 32.81831727339244 35.0 34.0 36.0 28.0 37.0 86-87 32.5956261369836 35.0 34.0 36.0 27.5 37.0 88-89 32.365712862489076 35.0 34.0 35.0 27.0 37.0 90-91 32.16980791933597 35.0 34.0 35.0 27.0 36.0 92-93 31.983695860421747 35.0 33.5 35.0 27.0 36.0 94-95 31.803019062094585 35.0 33.0 35.0 26.0 36.0 96-97 31.634238784346714 35.0 33.0 35.0 25.5 36.0 98-99 31.455268396554338 35.0 33.0 35.0 25.0 35.0 100 31.32579522708653 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3807423511356305 1101 2 0.4128497191555205 1101 3 0.39557449103528697 1101 4 0.34026911692207307 1101 5 0.31717720060092347 1101 6 0.2968414319582209 1101 7 0.3550973836999134 1101 8 0.32397467501026966 1101 9 0.342358647309716 1101 10-11 0.3706204945977376 1101 12-13 0.20689152064036875 1101 14-15 0.3056452693859413 1101 16-17 0.274659456702679 1101 18-19 0.2727637300563188 1101 20-21 0.27753403849730063 1101 22-23 0.2780109884407409 1101 24-25 0.2801793845165861 1101 26-27 0.3457692379549542 1101 28-29 0.352092999163105 1101 30-31 0.3460521761080315 1101 32-33 0.37640911637474517 1101 34-35 0.3236395376480914 1101 36-37 0.35945502114805805 1101 38-39 0.3785414699934009 1101 40-41 0.3707637767961458 1101 42-43 0.3058038924611495 1101 44-45 0.33906532329894645 1101 46-47 0.3070604032238222 1101 48-49 0.3068934210179748 1101 50-51 0.3203023372703342 1101 52-53 0.24868647787143772 1101 54-55 0.28545519431040844 1101 56-57 0.2939414792269446 1101 58-59 0.29537029979839957 1101 60-61 0.2941485475504564 1101 62-63 0.32595698681276275 1101 64-65 0.25640348628886045 1101 66-67 0.22982492774950458 1101 68-69 0.22154857329873323 1101 70-71 0.3003152040629402 1101 72-73 0.17755063461963516 1101 74-75 0.13907804709888438 1101 76-77 0.2089635853967522 1101 78-79 0.2235298282967335 1101 80-81 0.18732542061879798 1101 82-83 0.07993250908720029 1101 84-85 0.12828817022599992 1101 86-87 0.07392890134963892 1101 88-89 0.08496713670788125 1101 90-91 0.17986934567350232 1101 92-93 0.19883558755334718 1101 94-95 0.11246308836980745 1101 96-97 0.1486468457669261 1101 98-99 0.1989296010162782 1101 100 0.18999580736054256 1102 1 0.3281910430324473 1102 2 0.2922108894257569 1102 3 0.4209493395196837 1102 4 0.4473900171208385 1102 5 0.34823001757008143 1102 6 0.2942920478626547 1102 7 0.2702425056745952 1102 8 0.37268225989230785 1102 9 0.3471980444737284 1102 10-11 0.3601581111666903 1102 12-13 0.3815530803001508 1102 14-15 0.4702266201280807 1102 16-17 0.4588752681972963 1102 18-19 0.42874765275265503 1102 20-21 0.41002161014808536 1102 22-23 0.44604267684622556 1102 24-25 0.43905427666116736 1102 26-27 0.44980584293991654 1102 28-29 0.4757767559507826 1102 30-31 0.4478810933505457 1102 32-33 0.41327598836755186 1102 34-35 0.443051590519822 1102 36-37 0.39078245320468596 1102 38-39 0.3765401320681079 1102 40-41 0.4088453265990779 1102 42-43 0.45903896327202887 1102 44-45 0.44764835828776484 1102 46-47 0.4447660685382644 1102 48-49 0.45262271610599214 1102 50-51 0.44828432707901555 1102 52-53 0.42992714458448233 1102 54-55 0.46067231117018537 1102 56-57 0.4475253874192191 1102 58-59 0.44621797315518563 1102 60-61 0.5152646051700387 1102 62-63 0.46305954973891517 1102 64-65 0.4563919052430023 1102 66-67 0.49336546437002227 1102 68-69 0.44644445488642504 1102 70-71 0.508564904731756 1102 72-73 0.4709310864062246 1102 74-75 0.4915281598013266 1102 76-77 0.44380144335466554 1102 78-79 0.49505584753977416 1102 80-81 0.49936922731227185 1102 82-83 0.45467165805423093 1102 84-85 0.4511926669278097 1102 86-87 0.5102758842836863 1102 88-89 0.47244336411374377 1102 90-91 0.4621361374500026 1102 92-93 0.4680543516596458 1102 94-95 0.5020979043236835 1102 96-97 0.48005923015997354 1102 98-99 0.5497047170857421 1102 100 0.5097130374407719 1103 1 0.08926383256510206 1103 2 0.13908952185783008 1103 3 0.1243982673961348 1103 4 0.19949747934248308 1103 5 0.12920310031086046 1103 6 0.04225171548588236 1103 7 0.14829413870293706 1103 8 0.1524839569830121 1103 9 0.18853819873832833 1103 10-11 0.1805710238990983 1103 12-13 0.1850296260969202 1103 14-15 0.30004664760273414 1103 16-17 0.22241001730720455 1103 18-19 0.27764003868209386 1103 20-21 0.2249227477099467 1103 22-23 0.14786410493384494 1103 24-25 0.18352322781354502 1103 26-27 0.21579971465531145 1103 28-29 0.21396117628049183 1103 30-31 0.21785541379118456 1103 32-33 0.25500704188199563 1103 34-35 0.23518762530279247 1103 36-37 0.18344187381988064 1103 38-39 0.21937256895381552 1103 40-41 0.22286668374822227 1103 42-43 0.18499153151520176 1103 44-45 0.2551349991649374 1103 46-47 0.2811424087325989 1103 48-49 0.2012227946475491 1103 50-51 0.32516716319403116 1103 52-53 0.27792542339369675 1103 54-55 0.24745955481166249 1103 56-57 0.258229544384335 1103 58-59 0.29745078988304385 1103 60-61 0.29345717186423315 1103 62-63 0.2928202490411138 1103 64-65 0.23140334001240603 1103 66-67 0.27557950949390886 1103 68-69 0.22757176250587463 1103 70-71 0.29156684784464915 1103 72-73 0.28069638302120126 1103 74-75 0.31914396658539346 1103 76-77 0.2769929635608932 1103 78-79 0.297284905918346 1103 80-81 0.28708841618515635 1103 82-83 0.31561259223073534 1103 84-85 0.30494354434999593 1103 86-87 0.24799989955482715 1103 88-89 0.3469439240965144 1103 90-91 0.3666899098032985 1103 92-93 0.3498099350506223 1103 94-95 0.3347623860965214 1103 96-97 0.27506642223806566 1103 98-99 0.2724876076777072 1103 100 0.30118156822485886 1104 1 -0.003979253319730702 1104 2 0.04220428428181222 1104 3 0.03767714219667795 1104 4 7.063576089691992E-4 1104 5 0.06360427542755076 1104 6 -0.042141738880580704 1104 7 -0.05455138782995306 1104 8 -0.010378295443231877 1104 9 -0.018636156774363144 1104 10-11 0.05797860089876394 1104 12-13 0.06501870258895792 1104 14-15 0.06235315679488451 1104 16-17 0.08256738901990701 1104 18-19 0.0347454077665077 1104 20-21 0.01793707595566474 1104 22-23 0.06385819590408914 1104 24-25 0.08671800777764815 1104 26-27 0.1550434759087338 1104 28-29 0.1837106052088444 1104 30-31 0.07610274797342953 1104 32-33 0.040434370843044576 1104 34-35 0.0762836195527754 1104 36-37 0.04553560146593583 1104 38-39 0.12209913493477131 1104 40-41 0.08384842460228725 1104 42-43 0.03643977360010098 1104 44-45 0.04538219407896804 1104 46-47 0.04394797378630955 1104 48-49 0.02958318350469824 1104 50-51 0.10142273435101146 1104 52-53 0.076037297304417 1104 54-55 0.0651389556786981 1104 56-57 0.08282324889898973 1104 58-59 0.026453690458907886 1104 60-61 0.12410801568774588 1104 62-63 0.11311250531025507 1104 64-65 0.12121531489943749 1104 66-67 0.13193486577894475 1104 68-69 0.06325380768703326 1104 70-71 0.04738179273604004 1104 72-73 0.0083540636667081 1104 74-75 0.05580720157905006 1104 76-77 0.08042022960452044 1104 78-79 0.09686961659856763 1104 80-81 -0.010785392692930884 1104 82-83 -0.012648794196678637 1104 84-85 0.006832527158110224 1104 86-87 -0.02883721235410519 1104 88-89 -0.00952119500693982 1104 90-91 -0.05461398749297075 1104 92-93 -0.10323498422624056 1104 94-95 -0.04590654820211881 1104 96-97 -0.05502018655415952 1104 98-99 -0.009471929011478863 1104 100 -0.054915470194046634 1105 1 0.03419407039820399 1105 2 0.03888490160928981 1105 3 0.05308527548789499 1105 4 0.07506908025631986 1105 5 0.06935047960228502 1105 6 0.07682151578945451 1105 7 0.053557838855205375 1105 8 0.012263578898782157 1105 9 0.1105826361544402 1105 10-11 0.09274355263239187 1105 12-13 0.0978596631034776 1105 14-15 0.189928787297994 1105 16-17 0.09172430785917385 1105 18-19 0.12783297832511664 1105 20-21 0.12199994073863962 1105 22-23 0.1615060538418831 1105 24-25 0.11984985108826152 1105 26-27 0.17016508639208183 1105 28-29 0.20405801948204783 1105 30-31 0.18360248735024953 1105 32-33 0.19045362637310603 1105 34-35 0.19354556777511078 1105 36-37 0.1607747103780568 1105 38-39 0.14712572760918619 1105 40-41 0.13610304609218815 1105 42-43 0.1015677483647508 1105 44-45 0.09211837197273098 1105 46-47 0.0764842231838827 1105 48-49 0.06296146376135425 1105 50-51 -0.011053476056552825 1105 52-53 0.04826794666805512 1105 54-55 0.05264622286802023 1105 56-57 0.03313688823060801 1105 58-59 0.05063919585377619 1105 60-61 0.0706039972753203 1105 62-63 0.04672716200282423 1105 64-65 0.053453907250556654 1105 66-67 0.05955050405453477 1105 68-69 0.061974020995876344 1105 70-71 -0.050118118437339376 1105 72-73 -0.0030538780453284176 1105 74-75 0.037175247446647575 1105 76-77 -0.02251173883124835 1105 78-79 -0.049946251467929415 1105 80-81 -0.022439543590429878 1105 82-83 -0.0057663901069844314 1105 84-85 0.05369781276272079 1105 86-87 -0.004498651850418867 1105 88-89 -0.07189303900994304 1105 90-91 -0.05098488297078063 1105 92-93 0.007205794964818324 1105 94-95 0.03135865764724244 1105 96-97 -0.013671457386607244 1105 98-99 0.031142337364538974 1105 100 0.04302281501868421 1106 1 -0.0010320933703980018 1106 2 -0.043232698211500775 1106 3 0.013015183596390756 1106 4 0.0552558623904531 1106 5 0.05158159832068776 1106 6 -0.027918857708385758 1106 7 0.04760732290885983 1106 8 0.039230836914271094 1106 9 0.02920854328766609 1106 10-11 -0.0033078214346389245 1106 12-13 0.054617026793152945 1106 14-15 0.04941621404674379 1106 16-17 7.777782369302599E-4 1106 18-19 -0.02513964096107202 1106 20-21 0.05764091963369111 1106 22-23 0.08818936699448443 1106 24-25 0.08819215780259526 1106 26-27 0.10038436312613186 1106 28-29 0.15245883369842517 1106 30-31 0.1139693829368511 1106 32-33 0.10270276812325108 1106 34-35 0.08635976496905329 1106 36-37 0.11632919569692746 1106 38-39 0.08722396549524802 1106 40-41 0.12051852402728969 1106 42-43 0.1270477771947114 1106 44-45 0.01508208984210313 1106 46-47 0.09788280601829769 1106 48-49 0.1658082256741693 1106 50-51 0.07485088562538067 1106 52-53 0.10398170042726207 1106 54-55 0.10175255678702655 1106 56-57 -0.0015399488829359598 1106 58-59 -0.0728132818856082 1106 60-61 -0.02642747371087495 1106 62-63 -0.07121664146825424 1106 64-65 0.024091256567075447 1106 66-67 -0.03933496745791132 1106 68-69 0.03508201784126186 1106 70-71 -0.04454689068026596 1106 72-73 -0.04711300037990185 1106 74-75 -0.008681288187368352 1106 76-77 0.008359730294941414 1106 78-79 0.09865362062033256 1106 80-81 0.07487606293299365 1106 82-83 0.06459871929149585 1106 84-85 -0.0030889762849000135 1106 86-87 -0.03723731493781912 1106 88-89 -0.052096920327834084 1106 90-91 -0.06778769112421656 1106 92-93 -0.08266991365179877 1106 94-95 -0.06808745796562832 1106 96-97 -0.04693475172518902 1106 98-99 -0.10075769071994856 1106 100 -0.031441116344453235 1107 1 0.02908163639898831 1107 2 0.0580390224275078 1107 3 0.06477508060197579 1107 4 0.01961753674778066 1107 5 -0.04420890577360126 1107 6 0.05114529537438273 1107 7 0.01307514071497451 1107 8 0.0033155192495684105 1107 9 -0.06514855946524989 1107 10-11 -0.05416702349396729 1107 12-13 -0.038199432913309295 1107 14-15 -0.013570571032339274 1107 16-17 -0.029604202943502855 1107 18-19 -0.023255465416404775 1107 20-21 -0.002132057563791534 1107 22-23 0.05466844700427487 1107 24-25 0.04835683797656998 1107 26-27 0.07331795761439963 1107 28-29 0.045844569103707045 1107 30-31 -0.006687129356890864 1107 32-33 0.007971202292530677 1107 34-35 0.019027866194626597 1107 36-37 -0.029172694599985505 1107 38-39 -0.05611278422998822 1107 40-41 -0.0544757180584412 1107 42-43 -0.09530918712105318 1107 44-45 -0.06375701458018312 1107 46-47 -0.016453685373321036 1107 48-49 -0.04655666051925067 1107 50-51 0.02196215489805553 1107 52-53 0.024090043979065 1107 54-55 0.036255980310720304 1107 56-57 0.012435346324579655 1107 58-59 -0.028014472209676455 1107 60-61 0.01645060877448401 1107 62-63 -0.022230255102286378 1107 64-65 0.05012488678885063 1107 66-67 0.06311155604205254 1107 68-69 -2.89616162689299E-4 1107 70-71 -0.0016539167836100432 1107 72-73 -0.01746190496944422 1107 74-75 -0.06937853349405998 1107 76-77 -0.09683705440803436 1107 78-79 -0.03472895639313833 1107 80-81 -0.10739854893531486 1107 82-83 -0.15079044773062833 1107 84-85 -0.08973700730187772 1107 86-87 -0.03810554896182339 1107 88-89 -0.04294594207689073 1107 90-91 -0.02659136693665687 1107 92-93 0.025644591071340983 1107 94-95 -0.10895864319659765 1107 96-97 -0.1437042042492216 1107 98-99 -0.0644662619161096 1107 100 -0.0821927529723574 1108 1 -0.02106811573688816 1108 2 0.03833638153690089 1108 3 0.0544659957878757 1108 4 -0.09576289388841275 1108 5 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50-51 -0.15252006561518527 2214 52-53 -0.13240289321034737 2214 54-55 -0.0731600119415674 2214 56-57 -0.1528875935695524 2214 58-59 -0.20317377027861028 2214 60-61 -0.1816511261105589 2214 62-63 -0.14529312053558385 2214 64-65 -0.2159007818816292 2214 66-67 -0.13934619983759688 2214 68-69 -0.1949918039949381 2214 70-71 -0.13164712382187105 2214 72-73 -0.1400439034961849 2214 74-75 -0.13271982310980235 2214 76-77 -0.1343186978311408 2214 78-79 -0.11168501153952093 2214 80-81 -0.12127518625722189 2214 82-83 -0.11361327856344872 2214 84-85 -0.13486558124108683 2214 86-87 -0.16866477797935886 2214 88-89 -0.14392230880209667 2214 90-91 -0.1042531173271044 2214 92-93 -0.07246003549649771 2214 94-95 -0.1254888481944718 2214 96-97 -0.10883175612812934 2214 98-99 -0.12035431390657081 2214 100 -0.07966382679627415 2215 1 -0.18882605807901953 2215 2 -0.26593244598340604 2215 3 -0.3152980622720456 2215 4 -0.217890541768206 2215 5 -0.23417299888318155 2215 6 -0.23252802867602185 2215 7 -0.23349408891230183 2215 8 -0.25510760883732075 2215 9 -0.3138699873621391 2215 10-11 -0.28607170633230083 2215 12-13 -0.28313940609049837 2215 14-15 -0.3836792069124968 2215 16-17 -0.3888632898409341 2215 18-19 -0.36470625807281465 2215 20-21 -0.3784584060978844 2215 22-23 -0.3011473849827979 2215 24-25 -0.3509162607497842 2215 26-27 -0.3098842621655393 2215 28-29 -0.40020129121792536 2215 30-31 -0.3950667646179653 2215 32-33 -0.42199501075955226 2215 34-35 -0.4346198630962945 2215 36-37 -0.42147349481513174 2215 38-39 -0.4048961053151956 2215 40-41 -0.4313729791549079 2215 42-43 -0.36979991514156296 2215 44-45 -0.4418402095958527 2215 46-47 -0.40966602685855236 2215 48-49 -0.39847379486220547 2215 50-51 -0.3724721653582108 2215 52-53 -0.4045713162895268 2215 54-55 -0.3829734332210819 2215 56-57 -0.4014218540766379 2215 58-59 -0.4788965849470941 2215 60-61 -0.4710062199858527 2215 62-63 -0.4449458907566566 2215 64-65 -0.4201755397645712 2215 66-67 -0.47533421326389913 2215 68-69 -0.4760664312978804 2215 70-71 -0.43397174066166855 2215 72-73 -0.46338187548845156 2215 74-75 -0.49520320764276704 2215 76-77 -0.4728733196095405 2215 78-79 -0.4358497181112142 2215 80-81 -0.48294029042825315 2215 82-83 -0.500569592859442 2215 84-85 -0.4705391354443407 2215 86-87 -0.4736901197055303 2215 88-89 -0.4285533784698643 2215 90-91 -0.47295891055870243 2215 92-93 -0.4577266363151331 2215 94-95 -0.3853396856642348 2215 96-97 -0.39827643541194746 2215 98-99 -0.34287660039573 2215 100 -0.3166880259475029 2216 1 -1.0493623380369854E-4 2216 2 -0.053330294294838154 2216 3 -0.050094791924472304 2216 4 -0.024534788789218 2216 5 -0.016221185356222634 2216 6 -0.028097700919389013 2216 7 -0.047492448511135876 2216 8 -0.06709062732481641 2216 9 -0.032130153991047905 2216 10-11 -0.0927406900552441 2216 12-13 -0.04145389389452703 2216 14-15 -0.11882022789419722 2216 16-17 -0.0674636779115545 2216 18-19 -0.07328395060837067 2216 20-21 -0.13253840614766688 2216 22-23 -0.14843247749495703 2216 24-25 -0.1439794361084239 2216 26-27 -0.14933808823322892 2216 28-29 -0.1936258713474075 2216 30-31 -0.1921853589011988 2216 32-33 -0.2965186347766249 2216 34-35 -0.2427263759120848 2216 36-37 -0.17235188109848565 2216 38-39 -0.16908439300024014 2216 40-41 -0.08009485399156091 2216 42-43 -0.07651644311675199 2216 44-45 -0.13189882468903136 2216 46-47 -0.06145011870111006 2216 48-49 -0.03445844628318184 2216 50-51 -0.06252004565622116 2216 52-53 -0.0970449491433456 2216 54-55 -0.05356893120356432 2216 56-57 -0.05690587198911601 2216 58-59 -0.05001678104846263 2216 60-61 -4.26404138927694E-4 2216 62-63 -0.0013612843906969374 2216 64-65 -0.014188542246330371 2216 66-67 -0.052407086654298496 2216 68-69 -0.05779182714734077 2216 70-71 -0.05027484522666015 2216 72-73 -0.03464364642472617 2216 74-75 -0.04780572649244874 2216 76-77 -0.06962385043738095 2216 78-79 -0.006049677785711083 2216 80-81 0.0315176117500684 2216 82-83 -0.02176879083022243 2216 84-85 -0.08447498430838607 2216 86-87 -0.07220380174649677 2216 88-89 -0.05583926753493529 2216 90-91 -0.160465444781849 2216 92-93 -0.10992736267182579 2216 94-95 -0.15335236081253 2216 96-97 -0.08984150009453629 2216 98-99 -0.05127460179467391 2216 100 -0.025255450418107728 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 35785.0 3 5446.0 4 3602.0 5 3130.0 6 2502.0 7 2915.0 8 3303.0 9 3665.0 10 3877.0 11 4096.0 12 4495.0 13 4934.0 14 5283.0 15 5345.0 16 5293.0 17 5147.0 18 5015.0 19 4858.0 20 4839.0 21 5376.0 22 6329.0 23 9275.0 24 14543.0 25 19610.0 26 20278.0 27 17213.0 28 18123.0 29 22568.0 30 28084.0 31 33609.0 32 43695.0 33 59806.0 34 87281.0 35 138162.0 36 268987.0 37 562200.0 38 886089.0 39 384827.0 40 670.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.68903105971601 19.40060850704684 13.185159323155515 23.725201110081635 2 15.544125091030208 20.031978355809972 40.314787396525034 24.10910915663478 3 18.31169708645673 25.69319686275942 29.53312089698054 26.461985153803308 4 12.398174742076767 16.594047385998078 38.65820752484274 32.34957034708243 5 13.042222214917784 38.32434704705264 35.16174402250351 13.47168671552606 6 30.821805740397973 38.039449714178026 17.860184626532284 13.278559918891714 7 27.924893657661485 32.63609573239166 22.071107205713762 17.367903404233097 8 25.765982988828316 36.19371655278529 20.036134770202363 18.00416568818403 9 25.577882473247872 17.230524816478578 20.631423249698695 36.56016946057485 10-11 24.129313850576317 27.013525918483655 28.433773837351882 20.42338639358814 12-13 24.744726440311148 24.503230476443687 28.39311416030073 22.358928922944436 14-15 22.17412256058357 26.375434344083597 25.36450756259494 26.085935532737892 16-17 21.961024272364096 29.590806718386453 26.001865447479144 22.446303561770307 18-19 21.94976316817798 28.604792146751734 27.813532829601606 21.631911855468676 20-21 23.059883805116748 27.854050286597854 27.30744095350141 21.778624954783986 22-23 22.858053779144612 27.577232334099893 27.32060379833606 22.244110088419433 24-25 22.483516459437013 27.75434174303718 27.35110032700481 22.411041470520995 26-27 22.257316318712263 27.776045086652008 27.453624542307814 22.51301405232792 28-29 22.364748991716077 27.765130101858222 27.52072806790743 22.349392838518273 30-31 22.457006342385075 28.123208768790636 27.1666376630631 22.25314722576119 32-33 22.236197283554844 27.81788912541802 27.631051807183592 22.31486178384354 34-35 22.45702950106527 27.588272758679373 27.551899481206043 22.402798259049312 36-37 22.664093115281396 27.93038483856631 27.096932517129723 22.30858952902257 38-39 22.4230103044895 28.093474066773034 27.276148413571672 22.207367215165792 40-41 22.235298306672423 27.727862437562713 27.605594454117092 22.43124480164777 42-43 22.240324690348274 27.769106975543224 27.475711862515713 22.51485647159279 44-45 22.262801980357125 27.878829491485313 27.482586414189154 22.375782113968413 46-47 22.31347110329679 27.919308807835225 27.400078305503957 22.367141783364026 48-49 22.232619415077547 27.79085644806447 27.485788930532447 22.490735206325535 50-51 22.227952496352124 27.76424954207521 27.432206129161273 22.575591832411394 52-53 22.280700088352937 27.722404500119254 27.46121729818257 22.535678113345238 54-55 22.346543379557477 28.039191479865345 27.292448822834803 22.32181631774238 56-57 22.232481320871937 27.796043680872067 27.386485285153228 22.584989713102765 58-59 22.21501901130269 27.371509580158925 27.6637326872601 22.749738721278288 60-61 22.430991811072683 27.85789326728576 27.23523676633517 22.475878155306386 62-63 22.152455591035412 27.817997477736622 27.342233448514015 22.68731348271395 64-65 22.21886012111234 27.58520567567306 27.461485630304782 22.73444857290982 66-67 22.05760620995447 27.619217360641525 27.434213806111675 22.888962623292333 68-69 22.375047684405114 27.73953184642599 27.32409088277826 22.56132958639063 70-71 22.289357055335714 27.547263527147425 27.440109126567464 22.723270290949397 72-73 22.050232103573652 27.560676444824434 27.584579798580005 22.804511653021912 74-75 22.180593918238092 27.709677548946885 27.387339712160276 22.72238882065475 76-77 22.201224521621597 27.58907948324596 27.3246837577683 22.885012237364144 78-79 22.173437709142245 27.64580860908686 27.411121907251275 22.76963177451962 80-81 22.115219213428364 27.544926472333763 27.475754039775975 22.864100274461897 82-83 22.068406986968142 27.543138986102576 27.61870240194149 22.769751624987787 84-85 22.05509001893088 27.521526186686224 27.438859932468223 22.984523861914667 86-87 22.236384526777346 27.483274886435765 27.43451841387708 22.84582217290981 88-89 21.905642396189158 27.516941395034934 27.460772893914026 23.116643314861882 90-91 21.93123617162965 27.43587023348616 27.565021757457707 23.06787183742648 92-93 22.152697193932283 27.485385349286272 27.457466185217815 22.904451271563627 94-95 21.93213490630533 27.296352130699248 27.554290029858418 23.217222933137005 96-97 22.050372250982235 27.211617101067514 27.595198215225764 23.142812432724487 98-99 21.947973313242912 27.359531707231564 27.50285123509698 23.189643744428544 100 22.033249011159086 27.135379634204636 27.477963573843255 23.353407780793024 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2549.0 1 1572.0 2 571.5 3 485.5 4 399.5 5 336.0 6 278.5 7 260.5 8 281.5 9 285.5 10 262.5 11 265.0 12 294.0 13 307.5 14 332.0 15 415.0 16 507.0 17 591.5 18 762.0 19 1100.5 20 1561.5 21 2145.0 22 3222.5 23 4952.5 24 7045.0 25 9720.0 26 13512.0 27 18176.5 28 24211.5 29 31387.0 30 38253.5 31 45683.5 32 53335.0 33 60260.5 34 66664.0 35 73127.5 36 79448.5 37 84901.0 38 89556.0 39 91563.5 40 93576.5 41 96710.5 42 98164.0 43 99100.0 44 97979.5 45 96389.0 46 96793.0 47 96216.0 48 94333.0 49 91941.0 50 89731.5 51 87069.5 52 81525.0 53 76780.5 54 74254.5 55 71172.5 56 65862.0 57 59299.5 58 53992.5 59 50127.0 60 46130.0 61 40752.0 62 35085.5 63 29849.5 64 24753.5 65 19934.0 66 16006.0 67 12459.0 68 9353.0 69 6884.0 70 4941.0 71 3674.5 72 2871.5 73 2109.5 74 1563.0 75 1119.0 76 726.5 77 505.5 78 334.0 79 215.5 80 154.5 81 96.0 82 73.5 83 65.0 84 52.0 85 40.5 86 35.0 87 39.5 88 33.0 89 19.5 90 16.0 91 9.5 92 5.5 93 6.0 94 5.0 95 3.5 96 2.5 97 3.0 98 2.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0758323586673503 2 0.07856933022656651 3 0.07955463998788434 4 0.08141578064815136 5 0.0800655413456047 6 0.07981009066674452 7 0.07897075272191821 8 0.07926269635490128 9 0.07864231613481228 10-11 0.07814966125415336 12-13 0.07714610501577408 14-15 0.07570463332792021 16-17 0.07437264050243499 18-19 0.07389823209883752 20-21 0.07336908426405572 22-23 0.07502951367664688 24-25 0.07550392208024435 26-27 0.0752119784472613 28-29 0.07075983804426961 30-31 0.07625202763976345 32-33 0.07459159822717229 34-35 0.07229254211743068 36-37 0.06882571147575682 38-39 0.0705773732736552 40-41 0.07325960540168706 42-43 0.0780401823917847 44-45 0.07893425976779533 46-47 0.08251056927183785 48-49 0.08526578730811549 50-51 0.08612337173000323 52-53 0.08656128717947782 54-55 0.08626934354649476 56-57 0.08415275220736756 58-59 0.08314919596898829 60-61 0.08132454826284416 62-63 0.08167123132701154 64-65 0.08095961872161533 66-67 0.08026625259328056 68-69 0.08030274554740344 70-71 0.07845985136419786 72-73 0.0782956330706449 74-75 0.07787596409823173 76-77 0.07752928103406434 78-79 0.07796719648353895 80-81 0.07918971044665551 82-83 0.07873354852011948 84-85 0.07796719648353895 86-87 0.07871530204305803 88-89 0.07537619674081426 90-91 0.07293116881458113 92-93 0.07271221108984383 94-95 0.07294941529164256 96-97 0.07231078859449212 98-99 0.07181813371383321 100 0.07108827463137556 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2740255.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.60543424084579 #Duplication Level Percentage of deduplicated Percentage of total 1 78.69349070360947 48.47946666723823 2 12.621394845356187 15.550930203466812 3 3.791693131643582 7.007667056488059 4 1.6291205229309664 4.0145070898334385 5 0.8337386786523944 2.568141667088487 6 0.5084240673076749 1.8793011266991788 7 0.35442030380026884 1.5283951703571634 8 0.24079772281416328 1.1867558622538679 9 0.18500408689096157 1.025753139832397 >10 1.0528025441298785 11.664449945370613 >50 0.06672248425147134 2.7796299660421737 >100 0.022213660793209435 2.1919401416463575 >500 1.181652131066513E-4 0.05732700171626365 >1k 5.9082606552067824E-5 0.0657349619669445 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 7.298590824576545E-5 0.0 10-11 0.0 0.0 0.0 7.298590824576545E-5 0.0 12-13 0.0 0.0 0.0 7.298590824576545E-5 0.0 14-15 0.0 0.0 0.0 1.2772533943008953E-4 0.0 16-17 0.0 0.0 0.0 1.6421829355297226E-4 0.0 18-19 0.0 0.0 0.0 1.824647706144136E-4 0.0 20-21 0.0 0.0 0.0 1.824647706144136E-4 0.0 22-23 0.0 0.0 0.0 2.00711247675855E-4 0.0 24-25 0.0 0.0 0.0 3.1019011004450314E-4 0.0 26-27 0.0 0.0 0.0 4.926548806589168E-4 0.0 28-29 0.0 0.0 0.0 0.001769908274959812 0.0 30-31 0.0 0.0 0.0 0.005802379705538353 0.0 32-33 0.0 0.0 0.0 0.016166378676437047 0.0 34-35 0.0 0.0 0.0 0.030197919536685453 0.0 36-37 0.0 0.0 0.0 0.055633508560334714 0.0 38-39 0.0 0.0 0.0 0.10044685622323471 0.0 40-41 0.0 0.0 0.0 0.15555121694878762 0.0 42-43 0.0 0.0 0.0 0.20775438782157135 0.0 44-45 0.0 0.0 0.0 0.2717812758301691 0.0 46-47 0.0 0.0 0.0 0.3496937328825237 0.0 48-49 0.0 0.0 0.0 0.4423858363546458 0.0 50-51 0.0 0.0 0.0 0.5444201360822258 0.0 52-53 0.0 0.0 0.0 0.6468011188739734 0.0 54-55 0.0 0.0 0.0 0.755677847499594 0.0 56-57 0.0 0.0 0.0 0.8846257008928001 0.0 58-59 0.0 0.0 0.0 1.0293750034212144 0.0 60-61 0.0 0.0 0.0 1.1945421137813816 0.0 62-63 0.0 3.649295412288273E-5 0.0 1.3602201254992692 0.0 64-65 0.0 3.649295412288273E-5 0.0 1.530332031143087 0.0 66-67 0.0 3.649295412288273E-5 0.0 1.724018385150287 0.0 68-69 0.0 3.649295412288273E-5 0.0 1.9451291941808337 0.0 70-71 0.0 3.649295412288273E-5 0.0 2.1872599447861605 0.0 72-73 0.0 3.649295412288273E-5 0.0 2.4403568281054135 0.0 74-75 0.0 3.649295412288273E-5 0.0 2.700861781111612 0.0 76-77 0.0 3.649295412288273E-5 0.0 2.968008451768175 0.0 78-79 0.0 3.649295412288273E-5 0.0 3.275607562069953 0.0 80-81 0.0 3.649295412288273E-5 0.0 3.6100654866061737 0.0 82-83 0.0 3.649295412288273E-5 0.0 3.962058275598439 1.8246477061441364E-5 84-85 0.0 3.649295412288273E-5 0.0 4.312865043581711 3.649295412288273E-5 86-87 0.0 3.649295412288273E-5 0.0 4.675331310407243 3.649295412288273E-5 88 0.0 3.649295412288273E-5 0.0 4.96796830951864 3.649295412288273E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4505 0.0 35.785774 1 GGTATCA 2675 0.0 34.965553 1 ATCAACG 4705 0.0 31.867655 3 TCAACGC 4755 0.0 31.532557 4 CAACGCA 5015 0.0 30.178387 5 AACGCAG 5125 0.0 29.712992 6 TATCAAC 5520 0.0 28.354126 2 GTACATG 9990 0.0 28.27614 1 TACATGG 9965 0.0 27.356579 2 ACATGGG 10380 0.0 25.221846 3 ACGCAGA 6285 0.0 24.079409 7 CGCAGAG 6615 0.0 22.665018 8 CATGGGG 6785 0.0 21.059244 4 GCAGAGT 7485 0.0 18.963146 9 CATGGGA 3560 0.0 17.955933 4 GAGTACA 7490 0.0 16.943108 1 TATTACG 310 1.260978E-7 16.677927 2 AGTACAT 7420 0.0 15.96275 2 GTATAGG 1300 0.0 15.908176 1 GTATTAC 1005 0.0 15.900982 1 >>END_MODULE