FastQCFastQC Report
Wed 25 May 2016
SRR1032959_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032959_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2740255
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67220.24530563761401766No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52570.19184345982399448No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29190.10652293308469467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47100.052.270851
GTATCAA86050.043.1345631
TCAACGC102800.035.4300044
ATCAACG102900.035.3498463
CAACGCA105250.034.6027375
TATCAAC107350.034.103032
AACGCAG109050.033.5264056
ACGCAGA128450.028.5345577
CGCAGAG130550.027.9308878
GTGGTAT21600.026.7547281
GTACATG101900.026.1892661
GCAGAGT143500.025.2735989
TACATGG102500.024.9282262
ACATGGG103550.023.857923
TGGTATC22600.022.9033852
CAGAGTA139700.021.15182910-11
GAGTACT89950.021.07347112-13
AGAGTAC132100.020.18165410-11
TATAACG2351.2512828E-720.023852
GAGTACA78750.019.509511
Produced by FastQC (version 0.11.5)