FastQCFastQC Report
Wed 25 May 2016
SRR1032958_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032958_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2894093
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37200.038.1575051
GGTATCA22700.035.4067651
TCAACGC40100.033.52264
ATCAACG40700.032.7974363
CAACGCA41500.032.1646425
AACGCAG42750.031.2241576
GTACATG102400.030.8909361
TACATGG101800.029.5031972
TATCAAC47250.029.2456672
ACATGGG105300.027.8530143
ACGCAGA52500.025.1568077
CGCAGAG56450.023.2295748
GAGTACA69200.022.2783871
CATGGGG73400.021.5799224
CATGGGA33150.021.2678574
TATACCG1857.1847535E-620.3248215
AGTACAT70350.020.1771052
CTATACA11050.018.7157144
GCAGAGT68200.018.6760869
TACCTGG19300.018.5085452
Produced by FastQC (version 0.11.5)