FastQCFastQC Report
Wed 25 May 2016
SRR1032958_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032958_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2894093
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62750.21682095219469452No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50640.17497709990660285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40900.052.3828661
GTATCAA74950.043.379271
TCAACGC90350.035.4691054
ATCAACG90950.035.235113
CAACGCA93450.034.3351255
AACGCAG96100.033.3883176
TATCAAC98900.032.6865542
GTACATG104450.027.7539271
ACGCAGA115700.027.5681327
TACATGG103250.026.8887522
CGCAGAG119100.026.662638
CGACGCG1500.025.05107790-91
ACATGGG105000.024.963023
TAGACCG1151.3685632E-424.5463525
ACACCGT5750.024.5463526
GTGGTAT20050.023.9019621
GCAGAGT131900.023.8605359
TACACCG5700.022.2855035
TGGTATC20150.021.0172632
CATGGGG69350.020.6959134
Produced by FastQC (version 0.11.5)