FastQCFastQC Report
Wed 25 May 2016
SRR1032957_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032957_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2549536
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30850.035.803351
TCAACGC32150.033.4784664
ATCAACG33300.032.4634483
GTACATG80450.031.6653461
CAACGCA34350.031.3336665
AACGCAG35950.030.2006026
TACATGG80600.029.6237262
ACATGGG81350.028.3684063
TATCAAC39150.028.3329162
GGTATCA19650.027.746391
TACCGTC2104.9112714E-1126.8574287
ACGCAGA41900.025.4632787
CGCAGAG44700.023.8682638
TAGACCG1050.002336125422.381195
TACCTGG21050.022.3284682
GTACCTG21500.022.2983511
CATGGGG55100.022.178534
TACACCG2859.640644E-1121.4388245
GAGTACA54300.020.9472271
ACACCGT4100.020.6343656
Produced by FastQC (version 0.11.5)