FastQCFastQC Report
Wed 25 May 2016
SRR1032957_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032957_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2549536
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32260.12653282793418097No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26200.10276379702032057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29850.047.537261
GTATCAA54400.041.3721541
TCAACGC64050.034.4492154
ATCAACG64100.034.423023
CAACGCA65250.033.7376025
AACGCAG68300.032.299896
TATCAAC70700.031.4763532
GTACATG76450.029.0706771
TACATGG78050.027.3669012
ACGCAGA82050.026.8291937
CGCAGAG84500.025.9951298
ACATGGG80100.025.7849433
GCAGAGT95700.022.7754449
GTATAGA10150.022.2201351
GAGTACA55750.022.0814671
CATGGGG53500.021.36874
AGAGTAC85600.020.22201710-11
AGTACAT56750.020.062832
TATACCG2151.1859393E-619.6903255
GTGGTAT17450.019.386911
Produced by FastQC (version 0.11.5)