FastQCFastQC Report
Wed 25 May 2016
SRR1032956_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032956_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2924098
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG92150.029.4307141
TACATGG94100.027.7714182
ACATGGG95600.026.4507063
GTATCAA55250.023.1396581
TCAACGC55350.021.9935344
CATGGGG61500.021.628394
ATCAACG56350.021.6032313
GGTATCA40600.021.5331331
CAACGCA57200.021.1989465
AACGCAG57900.020.9426546
GAGTACA66450.019.663921
TATCAAC64600.019.4263692
ACACCGT4650.018.1932456
AGTACAT65550.017.9975722
ACGCAGA66600.017.9951957
TACACCG4253.6379788E-1217.693825
GTATAGA12650.017.4633541
CGCAGAG69150.016.991478
ACCGTTC4005.020411E-1016.4494448
CGCCGTA2901.1526045E-616.20468394
Produced by FastQC (version 0.11.5)