FastQCFastQC Report
Wed 25 May 2016
SRR1032956_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032956_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2924098
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54360.18590348203103998No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45900.15697148317190462No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52950.040.549821
GTATCAA89450.036.4517021
TCAACGC101850.031.688664
ATCAACG102300.031.6412473
CAACGCA103700.031.16395
AACGCAG106850.030.2886756
TATCAAC110150.029.4289992
GTACATG86900.026.9245381
ACGCAGA124000.025.9844177
CGCAGAG126750.025.3831068
TACATGG87400.024.9235762
ACATGGG86900.023.551053
GCAGAGT138550.023.0192179
GAGTACT84450.020.9963912-13
AGAGTAC115100.020.79199410-11
GTACTTT90450.019.31797614-15
AGTACTT88350.018.97968312-13
CAGAGTA136300.018.88478310-11
CATGGGG57900.018.6891734
GAGTACA63400.018.6745411
Produced by FastQC (version 0.11.5)