##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032956_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2924098 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.85644154197294 34.0 31.0 34.0 31.0 34.0 2 32.89801504600735 34.0 31.0 34.0 31.0 34.0 3 32.9772764113925 34.0 31.0 34.0 31.0 34.0 4 36.387148447145066 37.0 37.0 37.0 35.0 37.0 5 36.372940305010296 37.0 37.0 37.0 35.0 37.0 6 36.43026328118962 37.0 37.0 37.0 35.0 37.0 7 36.39317184307776 37.0 37.0 37.0 35.0 37.0 8 36.32485299740296 37.0 37.0 37.0 35.0 37.0 9 38.16849537874585 39.0 39.0 39.0 37.0 39.0 10-11 38.18588193692551 39.0 39.0 39.0 37.0 39.0 12-13 38.188848150780174 39.0 39.0 39.0 37.0 39.0 14-15 39.750767587132856 41.0 40.0 41.0 37.0 41.0 16-17 39.75996683421691 41.0 40.0 41.0 37.0 41.0 18-19 39.78206390483493 41.0 40.0 41.0 37.0 41.0 20-21 39.7922680771985 41.0 40.0 41.0 37.0 41.0 22-23 39.760414151646074 41.0 40.0 41.0 37.0 41.0 24-25 39.74550220273055 41.0 40.0 41.0 37.0 41.0 26-27 39.67629077411222 41.0 40.0 41.0 37.0 41.0 28-29 39.614691949449025 41.0 40.0 41.0 37.0 41.0 30-31 39.5099976129391 41.0 40.0 41.0 36.5 41.0 32-33 39.393326249667425 41.0 39.5 41.0 36.0 41.0 34-35 39.309542122049265 41.0 39.0 41.0 35.0 41.0 36-37 39.222979188796 41.0 39.0 41.0 35.0 41.0 38-39 39.127583446245644 41.0 39.0 41.0 35.0 41.0 40-41 39.02858556724159 41.0 39.0 41.0 35.0 41.0 42-43 38.90002746145991 41.0 39.0 41.0 35.0 41.0 44-45 38.73908415518221 41.0 39.0 41.0 35.0 41.0 46-47 38.52798469818727 41.0 39.0 41.0 35.0 41.0 48-49 38.33273765106368 41.0 38.0 41.0 35.0 41.0 50-51 38.18025490253747 41.0 38.0 41.0 34.0 41.0 52-53 38.04357685686321 40.0 38.0 41.0 34.0 41.0 54-55 37.89970154899049 40.0 37.5 41.0 34.0 41.0 56-57 37.690740016237484 40.0 37.0 41.0 33.5 41.0 58-59 37.52104153143978 40.0 37.0 41.0 33.0 41.0 60-61 37.32862407484291 40.0 36.0 41.0 33.0 41.0 62-63 37.09307417193268 39.0 36.0 41.0 33.0 41.0 64-65 36.80658445783965 39.0 35.0 41.0 32.5 41.0 66-67 36.52922097686192 39.0 35.0 41.0 32.0 41.0 68-69 36.232765796495194 37.5 35.0 40.5 32.0 41.0 70-71 35.904608874257974 37.0 35.0 40.0 32.0 41.0 72-73 35.56238932484479 37.0 35.0 39.0 31.0 41.0 74-75 35.2137566866774 36.0 35.0 39.0 31.0 41.0 76-77 34.8650746315616 36.0 35.0 39.0 31.0 40.5 78-79 34.51649688211544 35.0 35.0 37.0 31.0 39.5 80-81 34.18354463496094 35.0 35.0 37.0 31.0 39.0 82-83 33.879564569997314 35.0 34.0 36.5 30.0 39.0 84-85 33.605854352350704 35.0 34.0 36.0 30.0 37.0 86-87 33.45833928958605 35.0 34.0 36.0 30.0 37.0 88-89 33.27013407211386 35.0 34.0 35.5 30.0 37.0 90-91 33.10003392499157 35.0 34.0 35.0 30.0 36.0 92-93 32.94450698984781 35.0 34.0 35.0 30.0 36.0 94-95 32.84927728140438 35.0 34.0 35.0 30.0 36.0 96-97 32.79254320477631 35.0 34.0 35.0 30.0 36.0 98-99 32.684793396117364 35.0 34.0 35.0 30.0 36.0 100 32.60107971757444 35.0 34.0 35.0 30.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.20619812683627003 1101 2 0.19004660710115218 1101 3 0.19727037152699722 1101 4 0.11349692506205145 1101 5 0.09973052157979367 1101 6 0.07784577385792346 1101 7 0.06293933764738568 1101 8 0.03038215723071147 1101 9 0.09841485123687477 1101 10-11 0.14053656219043376 1101 12-13 0.18996993131482753 1101 14-15 0.2982332854147103 1101 16-17 0.2746232265527482 1101 18-19 0.24152328429031655 1101 20-21 0.29139585522531064 1101 22-23 0.26297829429483954 1101 24-25 0.2692804065084715 1101 26-27 0.3137197308247437 1101 28-29 0.2672429532946552 1101 30-31 0.2567548602857954 1101 32-33 0.2887237144370829 1101 34-35 0.23515987716834985 1101 36-37 0.2597298621586219 1101 38-39 0.30051033520604875 1101 40-41 0.3214182400726884 1101 42-43 0.2844014884655408 1101 44-45 0.2294831957303245 1101 46-47 0.26790839750140094 1101 48-49 0.2839831788585627 1101 50-51 0.2760859546332597 1101 52-53 0.28130404790813657 1101 54-55 0.2798646777638183 1101 56-57 0.26547667747082215 1101 58-59 0.2692320858624555 1101 60-61 0.2866643436816503 1101 62-63 0.2123511874365036 1101 64-65 0.18594124599297146 1101 66-67 0.15825063238683867 1101 68-69 0.19426015261050367 1101 70-71 0.13248615335722036 1101 72-73 0.1881641742093123 1101 74-75 0.1937192089626052 1101 76-77 0.18315456741382263 1101 78-79 0.1955055464957951 1101 80-81 0.21305317780622346 1101 82-83 0.2094140295718674 1101 84-85 0.23269166238267758 1101 86-87 0.23196736621445524 1101 88-89 0.17422078971348043 1101 90-91 0.18970650655283805 1101 92-93 0.23034731034549338 1101 94-95 0.2289066807795308 1101 96-97 0.23388380197646086 1101 98-99 0.18138138937074189 1101 100 0.21271537854138245 1102 1 0.21651323936735167 1102 2 0.1998984508683037 1102 3 0.19283636263865844 1102 4 0.11076456069513796 1102 5 0.11103194063272781 1102 6 0.13780423322587154 1102 7 0.13256478213498468 1102 8 -0.0030304822342088755 1102 9 0.11884828747682263 1102 10-11 0.16715744769761187 1102 12-13 0.18598342209812557 1102 14-15 0.29439932887740383 1102 16-17 0.24472916177921888 1102 18-19 0.24461833778001818 1102 20-21 0.2796085473247487 1102 22-23 0.25073120218633704 1102 24-25 0.2630015910023573 1102 26-27 0.2642364373216566 1102 28-29 0.276049784630807 1102 30-31 0.2995895619274833 1102 32-33 0.34929504223481445 1102 34-35 0.33338856515152315 1102 36-37 0.30538581387472874 1102 38-39 0.2602077245530907 1102 40-41 0.32093376867156564 1102 42-43 0.304954462693523 1102 44-45 0.3425556787469546 1102 46-47 0.35750347792259163 1102 48-49 0.37837596131548423 1102 50-51 0.36259501778616965 1102 52-53 0.3877331954274652 1102 54-55 0.35700400613987426 1102 56-57 0.36877078769713023 1102 58-59 0.36174114098316323 1102 60-61 0.40907171591538116 1102 62-63 0.3973465004134198 1102 64-65 0.423574970258052 1102 66-67 0.41031171771617636 1102 68-69 0.39010271115812856 1102 70-71 0.438559687174191 1102 72-73 0.4107215831549098 1102 74-75 0.4048111884833361 1102 76-77 0.3825592280724024 1102 78-79 0.4569188847618335 1102 80-81 0.4363446468572221 1102 82-83 0.4368130813946607 1102 84-85 0.39455607937275516 1102 86-87 0.39229408999476334 1102 88-89 0.39943413903561265 1102 90-91 0.35994407531080697 1102 92-93 0.36787145418418277 1102 94-95 0.333271612775178 1102 96-97 0.38709281816464625 1102 98-99 0.40971855453443595 1102 100 0.4252248636208691 1103 1 0.1252702882810084 1103 2 0.17638379768232681 1103 3 0.1525351790050422 1103 4 0.08522960365057486 1103 5 0.11339102685725777 1103 6 0.1062587746068786 1103 7 0.12612044903384145 1103 8 0.05113480961423278 1103 9 0.11984630366504945 1103 10-11 0.12898120686476489 1103 12-13 0.15842790659899464 1103 14-15 0.21068613836222028 1103 16-17 0.22029932095941263 1103 18-19 0.21934603890743887 1103 20-21 0.24552094798140445 1103 22-23 0.2107856042524361 1103 24-25 0.1862884317176139 1103 26-27 0.19288574801156244 1103 28-29 0.20581728080507133 1103 30-31 0.23292318380291732 1103 32-33 0.2808324047020747 1103 34-35 0.30463161128702865 1103 36-37 0.27785380606983523 1103 38-39 0.2727397866717851 1103 40-41 0.28110129621205715 1103 42-43 0.2523243845223817 1103 44-45 0.3077053098883198 1103 46-47 0.35190448829516896 1103 48-49 0.3799014154807452 1103 50-51 0.3906023562603096 1103 52-53 0.37655414113945795 1103 54-55 0.39581699148464367 1103 56-57 0.45416592403157807 1103 58-59 0.38481109988119044 1103 60-61 0.33676985607487353 1103 62-63 0.39473281822250783 1103 64-65 0.37836924992799226 1103 66-67 0.35713391875601275 1103 68-69 0.31307639883473115 1103 70-71 0.4040112691316864 1103 72-73 0.3372253287707707 1103 74-75 0.3229142007270269 1103 76-77 0.3699602072145396 1103 78-79 0.3510394661639751 1103 80-81 0.310485724931965 1103 82-83 0.28858663084262304 1103 84-85 0.27698255884688905 1103 86-87 0.2788840621725157 1103 88-89 0.2969022330690976 1103 90-91 0.25093609661268346 1103 92-93 0.3234024497155161 1103 94-95 0.3257213147960556 1103 96-97 0.29003667737782735 1103 98-99 0.309451873746184 1103 100 0.3315427134459057 1104 1 0.07945318761385778 1104 2 0.09648699767024738 1104 3 0.10749061723237219 1104 4 0.024015313812583372 1104 5 0.04948786990385656 1104 6 0.045869576587953986 1104 7 0.09592372392057769 1104 8 0.03329728941011467 1104 9 0.060834657359102096 1104 10-11 0.005844907202316563 1104 12-13 0.05834094350221619 1104 14-15 0.11888931734532093 1104 16-17 0.08495027223003149 1104 18-19 0.16325217022552607 1104 20-21 0.14677601976111987 1104 22-23 0.11599858179374678 1104 24-25 0.09362484179571595 1104 26-27 0.10593175531674603 1104 28-29 0.09601503189970373 1104 30-31 0.0938487378423929 1104 32-33 0.10833661561419206 1104 34-35 0.09184830863773641 1104 36-37 0.0817763367351958 1104 38-39 0.07158433648375961 1104 40-41 0.08058302297686737 1104 42-43 0.14361383540047257 1104 44-45 0.16177191679511083 1104 46-47 0.15471732906803481 1104 48-49 0.15253533044520395 1104 50-51 0.15132000460634032 1104 52-53 0.1488345232035826 1104 54-55 0.12544794947485371 1104 56-57 0.207672209911955 1104 58-59 0.22841727611459106 1104 60-61 0.19955370660476746 1104 62-63 0.15942840676974157 1104 64-65 0.12720922861679895 1104 66-67 0.13012223886852325 1104 68-69 0.12878264172420728 1104 70-71 0.07770389110994103 1104 72-73 0.06033612609076755 1104 74-75 0.11051294257512723 1104 76-77 0.12807626773174263 1104 78-79 0.1947126419246672 1104 80-81 0.19032428060315 1104 82-83 0.15940919804972253 1104 84-85 0.10283963898786652 1104 86-87 0.08683048959036199 1104 88-89 0.1130864131886895 1104 90-91 0.10307887941978322 1104 92-93 0.0637884945589704 1104 94-95 0.1278615980683142 1104 96-97 0.15718890799053042 1104 98-99 0.14116517050290156 1104 100 0.09749581061490176 1105 1 0.06480127910149491 1105 2 0.116867749200253 1105 3 0.08824478143058201 1105 4 0.022004634267169365 1105 5 0.06908347385714819 1105 6 0.09688077744016965 1105 7 0.09929159240270025 1105 8 0.04261495702184703 1105 9 0.10747567927848678 1105 10-11 0.11871333531874484 1105 12-13 0.11383409209783935 1105 14-15 0.1379551060530133 1105 16-17 0.1465875443756346 1105 18-19 0.14987579066571044 1105 20-21 0.17308228316093022 1105 22-23 0.15188087445838505 1105 24-25 0.15714961862029497 1105 26-27 0.1940149557769928 1105 28-29 0.18112328011128653 1105 30-31 0.17417776962630427 1105 32-33 0.16452189843037246 1105 34-35 0.16874266880410005 1105 36-37 0.15911253009971915 1105 38-39 0.16966082238595703 1105 40-41 0.13523157025892374 1105 42-43 0.1477657714401559 1105 44-45 0.08781360184625697 1105 46-47 0.16202165659938572 1105 48-49 0.1879926224052042 1105 50-51 0.17446679053521308 1105 52-53 0.1524203448176067 1105 54-55 0.16253754549237698 1105 56-57 0.19782728222187274 1105 58-59 0.15441144032333654 1105 60-61 0.18275287609638013 1105 62-63 0.12769731257073147 1105 64-65 0.14530026053294165 1105 66-67 0.17885481005956905 1105 68-69 0.14319939582413355 1105 70-71 0.12113928449637967 1105 72-73 0.12180096396014051 1105 74-75 0.11457979417970421 1105 76-77 0.12267509198602511 1105 78-79 0.1708481701027651 1105 80-81 0.1573171306129808 1105 82-83 0.15399053126223095 1105 84-85 0.1901646396661718 1105 86-87 0.10894295962312128 1105 88-89 0.0722744916739444 1105 90-91 0.08637100958369359 1105 92-93 0.1143736937131763 1105 94-95 0.16268336461611455 1105 96-97 0.07821761832386187 1105 98-99 0.004833800609247874 1105 100 0.014424057040500315 1106 1 0.08366765757069317 1106 2 0.11793348817563754 1106 3 0.10334862479746221 1106 4 0.07418651231758133 1106 5 0.061529963776372654 1106 6 0.1036053458120989 1106 7 0.05487077501847182 1106 8 0.06855373766719453 1106 9 0.12828746629608645 1106 10-11 0.11239196774393889 1106 12-13 0.07114273686210737 1106 14-15 0.12990425111705406 1106 16-17 0.10542231121655021 1106 18-19 0.12597908389242463 1106 20-21 0.1348499878662679 1106 22-23 0.14948108342088062 1106 24-25 0.16244041887505034 1106 26-27 0.11708710784360221 1106 28-29 0.16811274273377563 1106 30-31 0.17349836418107856 1106 32-33 0.19740295873350533 1106 34-35 0.1521592207111624 1106 36-37 0.18828692654654589 1106 38-39 0.1898308464539511 1106 40-41 0.1940517641845858 1106 42-43 0.2550164775205914 1106 44-45 0.3058253216973341 1106 46-47 0.29202524688162157 1106 48-49 0.29355725731837623 1106 50-51 0.25740268836546676 1106 52-53 0.2786415072118231 1106 54-55 0.25110801814395955 1106 56-57 0.26228263085996417 1106 58-59 0.2596819191772255 1106 60-61 0.2465224656998899 1106 62-63 0.27597344763191245 1106 64-65 0.30633763022763105 1106 66-67 0.22655791187180085 1106 68-69 0.30439931151300925 1106 70-71 0.2578824951062728 1106 72-73 0.2863149824490847 1106 74-75 0.26921554441506146 1106 76-77 0.2854290401619153 1106 78-79 0.2543580340502274 1106 80-81 0.30656423027508595 1106 82-83 0.3247147547250293 1106 84-85 0.3413127231765358 1106 86-87 0.3206692651470604 1106 88-89 0.26258509142756736 1106 90-91 0.32211128594465777 1106 92-93 0.30848706796834335 1106 94-95 0.30262477544576427 1106 96-97 0.24310644815911076 1106 98-99 0.2575748701603757 1106 100 0.27217813380093503 1107 1 0.02650444755912673 1107 2 0.056331961797425834 1107 3 0.010183247548297913 1107 4 0.001966719495683833 1107 5 0.031029156664864388 1107 6 0.03850896044101404 1107 7 0.012976080394089706 1107 8 0.025122628457104668 1107 9 0.06820395288284686 1107 10-11 9.792452132728613E-6 1107 12-13 0.019489025226214096 1107 14-15 0.08511972926602596 1107 16-17 0.08773741187631856 1107 18-19 0.0742798028886682 1107 20-21 0.08159639778733663 1107 22-23 0.06205532140300818 1107 24-25 0.09357892183703598 1107 26-27 0.10798357008612669 1107 28-29 0.11466385881380603 1107 30-31 0.15849667229452535 1107 32-33 0.16083872099383711 1107 34-35 0.1659556788869878 1107 36-37 0.14230154197344547 1107 38-39 0.20501870381850296 1107 40-41 0.15558752288050925 1107 42-43 0.1591128811484097 1107 44-45 0.19120075677975024 1107 46-47 0.1823635411180362 1107 48-49 0.20658448427913356 1107 50-51 0.2013758629560769 1107 52-53 0.18427408393426248 1107 54-55 0.1990647330885551 1107 56-57 0.2475893955372399 1107 58-59 0.2784287031196939 1107 60-61 0.26694104413073916 1107 62-63 0.24513117212515567 1107 64-65 0.2432520928293087 1107 66-67 0.13754555342059405 1107 68-69 0.18765341645093514 1107 70-71 0.20783984295070468 1107 72-73 0.2109038607799789 1107 74-75 0.16096820364245445 1107 76-77 0.19724272611860272 1107 78-79 0.19018342015341716 1107 80-81 0.22696724702036875 1107 82-83 0.26579764325592947 1107 84-85 0.2827798481739663 1107 86-87 0.2475347523905853 1107 88-89 0.19753142582954553 1107 90-91 0.20957289006948088 1107 92-93 0.2117419959078788 1107 94-95 0.1813901406783316 1107 96-97 0.2094763754094373 1107 98-99 0.2439467795695407 1107 100 0.19007478701461622 1108 1 0.06520653993663927 1108 2 0.07385073437805545 1108 3 0.05863027230767415 1108 4 0.008664880215356163 1108 5 0.028144230355486854 1108 6 0.05141217988061442 1108 7 3.4720856475445316E-4 1108 8 0.047174386732514506 1108 9 0.023729705208801022 1108 10-11 0.01701342311708487 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2214 52-53 -0.02308376314960725 2214 54-55 -0.02057212490446858 2214 56-57 -0.09549282562717565 2214 58-59 0.004484896299175034 2214 60-61 0.028384091875160777 2214 62-63 0.009755875106023382 2214 64-65 -0.019118301047932107 2214 66-67 -0.048027691056759636 2214 68-69 -0.12624325908958411 2214 70-71 -0.10011272801556004 2214 72-73 -0.1509370407869497 2214 74-75 -0.14183871286309113 2214 76-77 -0.08258112904772474 2214 78-79 -0.07517469725948445 2214 80-81 -0.06979624837326526 2214 82-83 -0.06598972140814396 2214 84-85 -0.10477149965135624 2214 86-87 -0.10618726708486292 2214 88-89 -0.13658135622844014 2214 90-91 -0.16273973566779887 2214 92-93 -0.1369166431617117 2214 94-95 -0.15706612380533613 2214 96-97 -0.13311141786329017 2214 98-99 -0.18257778805092073 2214 100 -0.17829397313496287 2215 1 -0.1379565633035682 2215 2 -0.21219210789855225 2215 3 -0.17210616609697382 2215 4 -0.1318439038587087 2215 5 -0.14545433934866736 2215 6 -0.1246368005924765 2215 7 -0.1360552431125086 2215 8 -0.09402941918192198 2215 9 -0.15090123082760698 2215 10-11 -0.15725205997629388 2215 12-13 -0.14232948069903983 2215 14-15 -0.28300416590700195 2215 16-17 -0.24938252515187997 2215 18-19 -0.250826317060195 2215 20-21 -0.24310173770614085 2215 22-23 -0.2181496030757728 2215 24-25 -0.26608285521383834 2215 26-27 -0.2811426072601009 2215 28-29 -0.27285062065787713 2215 30-31 -0.24335193697624646 2215 32-33 -0.25534501490424333 2215 34-35 -0.2632578370808858 2215 36-37 -0.20193928080336576 2215 38-39 -0.2426077029932472 2215 40-41 -0.2889883295278892 2215 42-43 -0.2751731035811389 2215 44-45 -0.22937060522088615 2215 46-47 -0.16651901227739785 2215 48-49 -0.21762679597638623 2215 50-51 -0.2343628255464978 2215 52-53 -0.25143114581231885 2215 54-55 -0.23347170975940656 2215 56-57 -0.22801264861939075 2215 58-59 -0.2099235324604578 2215 60-61 -0.24965527896868167 2215 62-63 -0.2425860622070104 2215 64-65 -0.2495530611019845 2215 66-67 -0.25732664518330495 2215 68-69 -0.22926643374918854 2215 70-71 -0.2717544115567847 2215 72-73 -0.28630020941700707 2215 74-75 -0.2752142251104459 2215 76-77 -0.33216487663824523 2215 78-79 -0.31398919443555684 2215 80-81 -0.3160513733407697 2215 82-83 -0.35536019612843006 2215 84-85 -0.38002285225969246 2215 86-87 -0.4402045811898816 2215 88-89 -0.4820853613298155 2215 90-91 -0.48653622500145843 2215 92-93 -0.45554974706490725 2215 94-95 -0.4964406986991463 2215 96-97 -0.5032093212280841 2215 98-99 -0.5542041084115468 2215 100 -0.4969563867943876 2216 1 -0.30082262325997533 2216 2 -0.3097425211839351 2216 3 -0.31923364169189483 2216 4 -0.19072791104643017 2216 5 -0.2457204270500455 2216 6 -0.2309480026574704 2216 7 -0.22034641642400743 2216 8 -0.20074831218783373 2216 9 -0.3829259840174686 2216 10-11 -0.4292281880463946 2216 12-13 -0.3929610683715268 2216 14-15 -0.5100115590114882 2216 16-17 -0.511105630762188 2216 18-19 -0.48765534375488073 2216 20-21 -0.4366020775928803 2216 22-23 -0.47301199602118515 2216 24-25 -0.45670838747039966 2216 26-27 -0.4785010997861576 2216 28-29 -0.45763375864020617 2216 30-31 -0.4685575733175682 2216 32-33 -0.5823554162389399 2216 34-35 -0.5874563006327094 2216 36-37 -0.6183478131830711 2216 38-39 -0.5954262307480676 2216 40-41 -0.5657272651381291 2216 42-43 -0.6288037669262039 2216 44-45 -0.6232551545393079 2216 46-47 -0.5920797063518535 2216 48-49 -0.642234725108537 2216 50-51 -0.6539626789872486 2216 52-53 -0.5952944102788251 2216 54-55 -0.6173669653475145 2216 56-57 -0.6634203622843557 2216 58-59 -0.6378212534988279 2216 60-61 -0.7262386090016193 2216 62-63 -0.5977773910591608 2216 64-65 -0.6209980309406973 2216 66-67 -0.6788774333071572 2216 68-69 -0.6102109401256612 2216 70-71 -0.6938520699633699 2216 72-73 -0.6468146773811938 2216 74-75 -0.7399876761586341 2216 76-77 -0.6557422380394513 2216 78-79 -0.6159741286126632 2216 80-81 -0.7243005898482977 2216 82-83 -0.7483117828832988 2216 84-85 -0.6776857820118849 2216 86-87 -0.6791485019353516 2216 88-89 -0.6725945681374199 2216 90-91 -0.6867262629116624 2216 92-93 -0.755255503194391 2216 94-95 -0.8153084051746475 2216 96-97 -0.7617693917981398 2216 98-99 -0.6809569489708025 2216 100 -0.7169531534879567 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13.0 3 6.0 4 13.0 5 27.0 6 44.0 7 143.0 8 328.0 9 600.0 10 986.0 11 1747.0 12 2723.0 13 4409.0 14 5178.0 15 6799.0 16 8443.0 17 12398.0 18 15027.0 19 8284.0 20 5516.0 21 5144.0 22 5366.0 23 5789.0 24 6353.0 25 7636.0 26 9747.0 27 12552.0 28 16087.0 29 21093.0 30 26712.0 31 33863.0 32 43379.0 33 57123.0 34 80870.0 35 124793.0 36 239675.0 37 565305.0 38 1042952.0 39 544789.0 40 2186.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.598615088612306 19.49692998085556 13.245506459112352 23.658948471419787 2 15.798149368537077 20.05784377529639 40.098759166548966 24.045247689617565 3 18.42308655131234 25.245477912459663 30.04802858459994 26.283406951628063 4 12.255384993195328 16.567210276472462 38.71818401649676 32.45922071383546 5 12.514957670519152 38.039113090077755 35.81198904956594 13.633940189837162 6 31.059252837299656 38.091139006735645 17.398319764568683 13.451288391396018 7 27.628159507754766 32.53258898296245 22.231539521441167 17.607711987841608 8 25.54579058423267 36.115967720226436 19.94625060833667 18.391991087204225 9 25.42321292996724 16.14751407289864 20.97118390183512 37.458089095299 10-11 24.15125963630494 26.469683984599694 28.762373901285116 20.61668247781025 12-13 25.10007369801597 23.857449911853067 29.017849967254826 22.024626422876135 14-15 22.098282492186033 25.740507778227872 26.386315202516748 25.774894527069346 16-17 21.33225357016078 29.339834027450518 26.93516085986174 22.39275154252696 18-19 21.2425895875476 27.79102594135963 29.6091440257584 21.35724044533437 20-21 22.26339541287604 27.09312410185979 29.352060019876212 21.291420465387958 22-23 21.933555510337385 26.85092310612343 29.463628917665126 21.751892465874057 24-25 21.704133961974755 27.023458803416524 29.423441567246627 21.848965667362098 26-27 21.550291543444573 26.97525001530479 29.557912132372934 21.916546308877702 28-29 21.56884037028968 27.09519189102947 29.575583481225078 21.76038425745578 30-31 21.779311387445123 27.38786719185026 29.200961392438835 21.631860028265788 32-33 21.574856691748817 27.156310745031888 29.550043388392293 21.718789174827002 34-35 21.68919918552833 26.957062360445615 29.541496236788568 21.812242217237486 36-37 21.92794038885594 27.29991903894974 29.028376736929275 21.743763835265046 38-39 21.73214356889303 27.517234312580197 29.104934507131762 21.64568761139501 40-41 21.677144076720474 27.176419967785066 29.27713141657606 21.869304538918396 42-43 21.700934761744325 27.180915385145827 29.069705961315588 22.048443891794257 44-45 21.79941522539978 27.34648896448062 28.961012573116086 21.893083237003523 46-47 21.914134706674048 27.580654493811718 28.61706247104778 21.88814832846645 48-49 21.931469571256436 27.498927109708017 28.457706724604172 22.11189659443138 50-51 21.89866977633083 27.55654554748429 28.34705166792375 22.19773300826113 52-53 21.977363412742836 27.554002399916467 28.287551759967272 22.18108242737343 54-55 21.99447331083672 27.884666741533643 28.13835279874662 21.98250714888302 56-57 21.857539078573417 27.54672278124557 28.258664519578826 22.337073620602187 58-59 21.86125477927208 27.153898551071894 28.597777831023425 22.387068838632608 60-61 22.019383850419715 27.535675520067254 28.260036879565657 22.18490374994737 62-63 21.72818435865102 27.51164153519023 28.408854412692936 22.351319693465815 64-65 21.794054098050065 27.295203512330986 28.496189252207003 22.414553137411946 66-67 21.70473111789193 27.419143855497296 28.294576360740365 22.58154866587041 68-69 22.06652927947646 27.50615916850883 28.19259354002057 22.234718011994133 70-71 22.023769727240627 27.31266655780117 28.173701458313204 22.489862256644997 72-73 21.83103986254907 27.483671887877904 28.24481258836058 22.44047566121245 74-75 21.90560986670077 27.601776684639162 28.04827334788369 22.444340100776376 76-77 21.97831022334591 27.500733987404313 27.907286917514522 22.61366887173525 78-79 22.009030136649 27.601524914952392 27.984651674576515 22.404793273822094 80-81 21.90853874050752 27.501474390071035 28.048242692898867 22.54174417652258 82-83 21.774499267721524 27.493418898341965 28.19440561473346 22.53767621920305 84-85 21.80886208328175 27.466367406290757 27.969479819075833 22.755290691351657 86-87 21.915206063315022 27.58797049462104 27.910858708577102 22.58596473348684 88-89 21.647361220428177 27.567650568715685 27.931832432568303 22.85315577828783 90-91 21.629377319629935 27.415035605063792 27.986067472215737 22.969519603090532 92-93 21.77615971179867 27.603471466283093 27.841735500588733 22.778633321329504 94-95 21.527197464765273 27.367672816146403 27.984193940737022 23.120935778351292 96-97 21.52154661423783 27.361088858227884 28.01216309823516 23.10520142929913 98-99 21.47810076067928 27.496112475064955 27.890114452427532 23.13567231182823 100 21.59366751729935 27.215845706949633 27.862164674371382 23.32832210137964 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 23.0 1 32.5 2 135.0 3 251.5 4 178.0 5 124.5 6 330.5 7 739.5 8 1273.0 9 1561.0 10 1275.5 11 963.5 12 1020.0 13 1157.0 14 1304.5 15 1525.0 16 1789.0 17 2058.5 18 2337.5 19 2680.0 20 3163.5 21 3885.5 22 5046.0 23 6883.0 24 9255.0 25 12329.5 26 16555.0 27 21721.0 28 28608.0 29 36414.0 30 44045.0 31 52126.5 32 60088.5 33 67833.0 34 74917.5 35 80765.0 36 86645.0 37 92578.5 38 97073.5 39 100152.5 40 102545.5 41 104447.5 42 104903.5 43 105312.0 44 105099.5 45 103809.5 46 102887.0 47 101013.0 48 100092.5 49 97693.0 50 93772.0 51 91236.5 52 85144.5 53 79460.0 54 76819.0 55 72858.0 56 67331.0 57 61087.5 58 55263.5 59 50605.5 60 45681.0 61 39357.5 62 33620.0 63 28372.0 64 23107.0 65 18285.5 66 14044.5 67 10581.0 68 8108.5 69 6195.5 70 4348.0 71 3100.5 72 2328.0 73 1610.5 74 1138.0 75 782.5 76 492.5 77 321.5 78 198.0 79 95.0 80 50.0 81 27.5 82 15.5 83 9.5 84 3.5 85 2.5 86 1.5 87 2.5 88 2.5 89 0.5 90 0.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.1038296254092716E-4 2 5.129787031761589E-4 3 2.0519148127046358E-4 4 0.012687673258556997 5 4.4458154275267103E-4 6 0.003522453761809625 7 9.233616657170861E-4 8 0.14537816448012345 9 0.0012995460480462694 10-11 0.0 12-13 1.0259574063523179E-4 14-15 2.2229077137633552E-4 16-17 0.0 18-19 1.0259574063523179E-4 20-21 0.0 22-23 1.0259574063523179E-4 24-25 4.95879413070287E-4 26-27 0.006053148697478676 28-29 0.007694680547642384 30-31 0.01443180084935594 32-33 0.017270283006930686 34-35 0.024982062844678937 36-37 0.03719095598027152 38-39 0.04180776430885695 40-41 0.05268291281619152 42-43 0.06896143699698164 44-45 0.10750323689561704 46-47 0.11412066216658949 48-49 0.1111282863980619 50-51 0.11323149908108415 52-53 0.10817010920974605 54-55 0.11497562667188309 56-57 0.10027023718083321 58-59 0.08987386879646304 60-61 0.09223357083107338 62-63 0.09103662052366233 64-65 0.0 66-67 1.7099290105871966E-5 68-69 6.839716042348786E-5 70-71 1.7099290105871966E-5 72-73 0.0 74-75 0.0 76-77 5.1297870317615895E-5 78-79 1.7099290105871966E-5 80-81 2.5648935158807944E-4 82-83 1.880921911645916E-4 84-85 0.0 86-87 7.181701844466225E-4 88-89 3.2488651201156735E-4 90-91 1.880921911645916E-4 92-93 0.0010601559865640617 94-95 0.001008858116246446 96-97 2.5648935158807944E-4 98-99 3.419858021174393E-5 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2924098.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.90249453673725 #Duplication Level Percentage of deduplicated Percentage of total 1 75.86472727988442 46.20351138694222 2 14.419779806493432 17.564011217718406 3 4.467510289570434 8.162475630101424 4 1.8563117636839752 4.522160681849775 5 0.9853794417311235 3.0006033033321478 6 0.580826004729782 2.1224251547910282 7 0.3771764135743228 1.607968912696744 8 0.25193154082961244 1.2274607431205813 9 0.18779762478785017 1.029360943588885 >10 0.9403788923172754 10.125386457250748 >50 0.05040056702907811 2.1017793685837716 >100 0.017162051297539244 1.6892214267101329 >500 3.3726767518005106E-4 0.13186964242040583 >1k 2.248451167429084E-4 0.3256687196596734 >5k 5.62112791857271E-5 0.1860964112340991 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5436 0.18590348203103998 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4590 0.15697148317190462 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.419858021174393E-5 0.0 5 0.0 0.0 0.0 3.419858021174393E-5 0.0 6 0.0 0.0 0.0 3.419858021174393E-5 0.0 7 0.0 0.0 0.0 1.0259574063523179E-4 0.0 8 0.0 0.0 0.0 1.0259574063523179E-4 0.0 9 0.0 0.0 0.0 1.3679432084697573E-4 0.0 10-11 1.7099290105871966E-5 0.0 0.0 1.8809219116459162E-4 0.0 12-13 3.419858021174393E-5 0.0 0.0 2.393900614822075E-4 0.0 14-15 3.419858021174393E-5 0.0 0.0 2.906879317998234E-4 0.0 16-17 3.419858021174393E-5 0.0 0.0 3.077872219056954E-4 0.0 18-19 3.419858021174393E-5 0.0 0.0 3.419858021174393E-4 0.0 20-21 3.419858021174393E-5 0.0 0.0 5.12978703176159E-4 0.0 22-23 3.419858021174393E-5 0.0 0.0 6.668723141290067E-4 0.0 24-25 3.419858021174393E-5 0.0 0.0 7.865673448701104E-4 0.0 26-27 3.419858021174393E-5 0.0 0.0 9.74659536034702E-4 0.0 28-29 3.419858021174393E-5 0.0 0.0 0.0022229077137633554 0.0 30-31 3.419858021174393E-5 0.0 0.0 0.006343836629278499 0.0 32-33 3.419858021174393E-5 0.0 0.0 0.015885240508355054 0.0 34-35 3.419858021174393E-5 0.0 0.0 0.028504516606488565 0.0 36-37 3.419858021174393E-5 3.419858021174393E-5 0.0 0.05418765034550826 0.0 38-39 3.419858021174393E-5 3.419858021174393E-5 0.0 0.09864580462077537 0.0 40-41 3.419858021174393E-5 3.419858021174393E-5 0.0 0.15161940536876672 0.0 42-43 3.419858021174393E-5 3.419858021174393E-5 0.0 0.2070553038920036 0.0 44-45 3.419858021174393E-5 3.419858021174393E-5 0.0 0.27201550700421123 0.0 46-47 3.419858021174393E-5 3.419858021174393E-5 0.0 0.35316873784667957 0.0 48-49 3.419858021174393E-5 3.419858021174393E-5 0.0 0.4438633725682245 0.0 50-51 3.419858021174393E-5 3.419858021174393E-5 0.0 0.543158950213023 0.0 52-53 3.419858021174393E-5 3.419858021174393E-5 0.0 0.6446774355715849 0.0 54-55 3.419858021174393E-5 3.419858021174393E-5 0.0 0.7532750270339776 0.0 56-57 3.419858021174393E-5 3.419858021174393E-5 0.0 0.8843239864053805 0.0 58-59 3.419858021174393E-5 3.419858021174393E-5 0.0 1.0316514699575732 0.0 60-61 3.419858021174393E-5 3.419858021174393E-5 0.0 1.192897775655946 0.0 62-63 3.419858021174393E-5 3.419858021174393E-5 0.0 1.3565721805493522 0.0 64-65 3.419858021174393E-5 3.419858021174393E-5 0.0 1.5283345496628362 0.0 66-67 3.419858021174393E-5 3.419858021174393E-5 0.0 1.7207699605143194 0.0 68-69 3.419858021174393E-5 3.419858021174393E-5 0.0 1.9443089800683837 0.0 70-71 3.419858021174393E-5 3.419858021174393E-5 0.0 2.1898547859887048 0.0 72-73 3.419858021174393E-5 3.419858021174393E-5 0.0 2.4381364783259656 0.0 74-75 3.419858021174393E-5 3.419858021174393E-5 0.0 2.690026120875566 0.0 76-77 3.419858021174393E-5 3.419858021174393E-5 0.0 2.959203145722202 0.0 78-79 3.419858021174393E-5 3.419858021174393E-5 0.0 3.269521062563567 0.0 80-81 3.419858021174393E-5 3.419858021174393E-5 0.0 3.612173736995135 0.0 82-83 3.419858021174393E-5 3.419858021174393E-5 0.0 3.9698908860099764 0.0 84-85 3.419858021174393E-5 3.419858021174393E-5 0.0 4.316185709234095 0.0 86-87 3.419858021174393E-5 3.419858021174393E-5 0.0 4.6776818013623345 0.0 88 3.419858021174393E-5 3.419858021174393E-5 0.0 4.974799066241966 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5295 0.0 40.54982 1 GTATCAA 8945 0.0 36.451702 1 TCAACGC 10185 0.0 31.68866 4 ATCAACG 10230 0.0 31.641247 3 CAACGCA 10370 0.0 31.1639 5 AACGCAG 10685 0.0 30.288675 6 TATCAAC 11015 0.0 29.428999 2 GTACATG 8690 0.0 26.924538 1 ACGCAGA 12400 0.0 25.984417 7 CGCAGAG 12675 0.0 25.383106 8 TACATGG 8740 0.0 24.923576 2 ACATGGG 8690 0.0 23.55105 3 GCAGAGT 13855 0.0 23.019217 9 GAGTACT 8445 0.0 20.99639 12-13 AGAGTAC 11510 0.0 20.791994 10-11 GTACTTT 9045 0.0 19.317976 14-15 AGTACTT 8835 0.0 18.979683 12-13 CAGAGTA 13630 0.0 18.884783 10-11 CATGGGG 5790 0.0 18.689173 4 GAGTACA 6340 0.0 18.674541 1 >>END_MODULE