FastQCFastQC Report
Wed 25 May 2016
SRR1032954_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032954_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1784724
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG67550.029.1543851
TACATGG68250.027.6846222
ACATGGG67900.026.1659953
TAACCGT1201.8387997E-423.4996227
CATGGGG50650.021.4361974
CAGCGTT5050.021.4055969
TACCTGG14100.020.6674922
GAGTACA47300.020.5695341
GTATCAA45550.020.3279251
GTACCTG16250.019.9577221
AGTACAT46650.019.7478432
TCAACGC46900.019.4421424
CTACACT10500.019.2483884
ATCAACG47600.019.1562293
TACACTG14000.019.1359425
CAACGCA48050.018.9762945
GTACAAG12050.018.722741
GGTATCA37300.018.523531
AACGCAG49100.018.3785236
GTCTAGA7200.018.278511
Produced by FastQC (version 0.11.5)