##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032954_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1784724 Sequences flagged as poor quality 0 Sequence length 100 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.164152552439482 31.0 31.0 34.0 28.0 34.0 2 31.30466951752764 33.0 31.0 34.0 28.0 34.0 3 31.512529668452938 34.0 31.0 34.0 28.0 34.0 4 34.86621853014808 37.0 35.0 37.0 32.0 37.0 5 34.97431703725618 37.0 35.0 37.0 33.0 37.0 6 35.02212947212006 37.0 35.0 37.0 33.0 37.0 7 34.937668233295454 37.0 35.0 37.0 32.0 37.0 8 34.967058211801934 37.0 35.0 37.0 33.0 37.0 9 36.586012739224664 39.0 38.0 39.0 33.0 39.0 10-11 36.573273234404866 39.0 38.0 39.0 33.0 39.0 12-13 36.52442870718386 39.0 38.0 39.0 33.0 39.0 14-15 37.9575281668202 41.0 39.0 41.0 33.0 41.0 16-17 37.968287813689955 41.0 39.0 41.0 33.0 41.0 18-19 37.95253607840764 41.0 39.0 41.0 33.0 41.0 20-21 37.97316363762688 41.0 39.0 41.0 33.0 41.0 22-23 37.956679576225795 41.0 39.0 41.0 33.0 41.0 24-25 37.97303868833501 41.0 39.0 41.0 33.0 41.0 26-27 37.936809837263354 41.0 39.0 41.0 33.0 41.0 28-29 37.92008624302693 41.0 39.0 41.0 33.0 41.0 30-31 37.86690855280705 41.0 38.0 41.0 33.0 41.0 32-33 37.81204460745751 41.0 38.0 41.0 32.0 41.0 34-35 37.74538584117208 40.5 38.0 41.0 32.0 41.0 36-37 37.65204843998288 40.0 38.0 41.0 32.0 41.0 38-39 37.549231421777264 40.0 38.0 41.0 32.0 41.0 40-41 37.44969362209507 40.0 38.0 41.0 31.5 41.0 42-43 37.321636846929835 40.0 38.0 41.0 31.0 41.0 44-45 37.16124313899516 40.0 37.5 41.0 31.0 41.0 46-47 37.00751068512554 40.0 37.0 41.0 30.5 41.0 48-49 36.850364538158274 40.0 37.0 41.0 30.0 41.0 50-51 36.67740222017522 40.0 36.0 41.0 30.0 41.0 52-53 36.49037834421457 40.0 36.0 41.0 30.0 41.0 54-55 36.29093181914963 39.5 35.0 41.0 30.0 41.0 56-57 36.07417253311996 39.0 35.0 41.0 30.0 41.0 58-59 35.83324536454936 39.0 35.0 41.0 29.0 41.0 60-61 35.552901457032014 38.5 35.0 41.0 29.0 41.0 62-63 35.274140707470735 38.0 35.0 40.0 28.5 41.0 64-65 34.973925380058766 37.0 34.5 40.0 28.0 41.0 66-67 34.65194142063423 37.0 34.0 40.0 28.0 41.0 68-69 34.32439777803178 36.0 34.0 39.0 28.0 41.0 70-71 33.979146635558216 36.0 34.0 39.0 27.0 41.0 72-73 33.69163019043841 35.0 34.0 39.0 27.0 40.5 74-75 33.42571736582239 35.0 34.0 37.5 27.0 40.0 76-77 33.119269702206054 35.0 34.0 37.0 26.5 39.0 78-79 32.779435083519914 35.0 33.0 37.0 26.0 39.0 80-81 32.49029345713959 35.0 33.0 36.0 26.0 39.0 82-83 32.211362092962275 35.0 33.0 36.0 26.0 37.0 84-85 32.08905130429131 35.0 33.0 35.5 26.0 37.0 86-87 31.898600848086314 35.0 33.0 35.0 25.5 37.0 88-89 31.699620221389974 35.0 33.0 35.0 25.0 36.0 90-91 31.5290941344432 35.0 33.0 35.0 25.0 36.0 92-93 31.348382999276076 35.0 33.0 35.0 24.0 36.0 94-95 31.182900829483998 35.0 33.0 35.0 24.0 35.5 96-97 31.01980278183069 35.0 33.0 35.0 23.0 35.0 98-99 30.843444420537853 35.0 33.0 35.0 21.0 35.0 100 30.704993040940785 35.0 33.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4637501174991421 1101 2 0.4524469656628973 1101 3 0.5071908479052532 1101 4 0.433285872245321 1101 5 0.4147160411089885 1101 6 0.4064033782359999 1101 7 0.5274132315740943 1101 8 0.42555533557514025 1101 9 0.46944484791657004 1101 10-11 0.47179198860810345 1101 12-13 0.380954826932971 1101 14-15 0.4774502632412094 1101 16-17 0.47555435816322245 1101 18-19 0.48429211692567975 1101 20-21 0.49838352366497674 1101 22-23 0.5000973975187435 1101 24-25 0.5618187634291445 1101 26-27 0.5906317701537986 1101 28-29 0.5004298079093417 1101 30-31 0.5459718085270424 1101 32-33 0.5578457843885545 1101 34-35 0.6116994786146819 1101 36-37 0.6350885151764203 1101 38-39 0.5515321080917985 1101 40-41 0.590642083310442 1101 42-43 0.6336917848148076 1101 44-45 0.5157513887936389 1101 46-47 0.4831211689181103 1101 48-49 0.5501421718587238 1101 50-51 0.553140902625131 1101 52-53 0.37448590935568404 1101 54-55 0.5089973947850908 1101 56-57 0.48640598495533993 1101 58-59 0.4363346083420794 1101 60-61 0.42922746610403806 1101 62-63 0.433508706679234 1101 64-65 0.4450413235706563 1101 66-67 0.42449231448641456 1101 68-69 0.43477981317261793 1101 70-71 0.5404778716690473 1101 72-73 0.5163274169801113 1101 74-75 0.4839594341973239 1101 76-77 0.4026674996385893 1101 78-79 0.47836806336323434 1101 80-81 0.4636163097838093 1101 82-83 0.44720150829923 1101 84-85 0.4045591002688198 1101 86-87 0.3556114927113114 1101 88-89 0.44600187477107056 1101 90-91 0.34579981486382394 1101 92-93 0.38020433394589404 1101 94-95 0.38585109989434585 1101 96-97 0.38448591962934486 1101 98-99 0.3673649178415843 1101 100 0.4340153912644027 1102 1 0.41363227789193857 1102 2 0.48358542720135844 1102 3 0.5520085238463359 1102 4 0.4627444647166783 1102 5 0.5258499199960553 1102 6 0.30540239623927334 1102 7 0.4284148682353077 1102 8 0.4189150737093428 1102 9 0.5693826547905445 1102 10-11 0.5074734943364163 1102 12-13 0.5458212753781453 1102 14-15 0.6809239129957092 1102 16-17 0.6767622141370353 1102 18-19 0.5724099565328729 1102 20-21 0.5968535727648145 1102 22-23 0.63805157100483 1102 24-25 0.694061971285123 1102 26-27 0.6975957636071541 1102 28-29 0.6780209699388067 1102 30-31 0.7118546235843297 1102 32-33 0.7216238531283281 1102 34-35 0.8501142085655786 1102 36-37 0.749378858875275 1102 38-39 0.8177880819052277 1102 40-41 0.775152394276077 1102 42-43 0.8531160074007289 1102 44-45 0.8960034346201482 1102 46-47 0.824531316217616 1102 48-49 0.8760266235772178 1102 50-51 0.8596639795482162 1102 52-53 0.8625035852967713 1102 54-55 0.9000831558325544 1102 56-57 0.9110030389651627 1102 58-59 0.8305107130884011 1102 60-61 0.9152238654493772 1102 62-63 0.8712596068428979 1102 64-65 0.9179041713611653 1102 66-67 0.8710631819168597 1102 68-69 0.7957087820760549 1102 70-71 0.8331868733057135 1102 72-73 0.8107012304007313 1102 74-75 0.8245414309240005 1102 76-77 0.726829856430733 1102 78-79 0.7255061975694446 1102 80-81 0.7382100904712061 1102 82-83 0.765449298806594 1102 84-85 0.6934867598432888 1102 86-87 0.6766625565410962 1102 88-89 0.7319184050493064 1102 90-91 0.7343700276297795 1102 92-93 0.7574603077593096 1102 94-95 0.7424144386832126 1102 96-97 0.7893199621825353 1102 98-99 0.7293478965649882 1102 100 0.7178451784984432 1103 1 0.10353487019358809 1103 2 0.19933499515108366 1103 3 0.21442423476542416 1103 4 0.19902443329483788 1103 5 0.16680011638254655 1103 6 0.23497725876872977 1103 7 0.2991799899949257 1103 8 0.22014021117988847 1103 9 0.3200378001165447 1103 10-11 0.3451913262295818 1103 12-13 0.33585568229094775 1103 14-15 0.4391814149595774 1103 16-17 0.4252233296122938 1103 18-19 0.3933094066507081 1103 20-21 0.402153179991835 1103 22-23 0.3810052237935153 1103 24-25 0.3212805101696503 1103 26-27 0.3956397541805998 1103 28-29 0.40682882539834964 1103 30-31 0.37339099436201195 1103 32-33 0.32320185784160316 1103 34-35 0.3606246563267774 1103 36-37 0.31222237812114884 1103 38-39 0.36357111411260945 1103 40-41 0.34299261422894745 1103 42-43 0.22296471124990802 1103 44-45 0.24067193422737176 1103 46-47 0.2472581033346657 1103 48-49 0.3349133478505877 1103 50-51 0.35168613194412046 1103 52-53 0.312964386553638 1103 54-55 0.2986525195403331 1103 56-57 0.37334522278291615 1103 58-59 0.39065305401534545 1103 60-61 0.4258614145459987 1103 62-63 0.42779582051139897 1103 64-65 0.34080236460545876 1103 66-67 0.378226261256998 1103 68-69 0.3188338409919993 1103 70-71 0.2723317279797115 1103 72-73 0.35865236944236045 1103 74-75 0.26367277420758484 1103 76-77 0.24580660427048429 1103 78-79 0.18431593833928162 1103 80-81 0.15236767098966908 1103 82-83 0.14922840328420506 1103 84-85 0.22471503949765292 1103 86-87 0.16470293360315225 1103 88-89 0.2174761426769507 1103 90-91 0.28835258850959633 1103 92-93 0.19607287649458272 1103 94-95 0.19322857851039288 1103 96-97 0.18468147043076755 1103 98-99 0.21913281408266272 1103 100 0.20935342673096713 1104 1 -0.0393905531582881 1104 2 0.09358968899740105 1104 3 0.147330898275559 1104 4 0.06520960931332809 1104 5 0.1395582913811424 1104 6 0.04537073718295659 1104 7 0.0581043659521967 1104 8 0.1567025927352148 1104 9 0.1524968100416828 1104 10-11 0.18597912599547328 1104 12-13 0.1768246239321769 1104 14-15 0.23545389777500958 1104 16-17 0.2523238579726552 1104 18-19 0.24428210265159578 1104 20-21 0.21025174628079668 1104 22-23 0.248622347260536 1104 24-25 0.30170702455757237 1104 26-27 0.23544203453561607 1104 28-29 0.33998687557046736 1104 30-31 0.3079995246497731 1104 32-33 0.29647042847079064 1104 34-35 0.3414965525534015 1104 36-37 0.3991782500472709 1104 38-39 0.4427975187393187 1104 40-41 0.34038892395643927 1104 42-43 0.3282934807645077 1104 44-45 0.2503672093537901 1104 46-47 0.2334518641628236 1104 48-49 0.2498244926791955 1104 50-51 0.3254597177521674 1104 52-53 0.22048592928458532 1104 54-55 0.24377295796345066 1104 56-57 0.19002800091340788 1104 58-59 0.23200355935932748 1104 60-61 0.27064913181163064 1104 62-63 0.19922825220059082 1104 64-65 0.1966782961709015 1104 66-67 0.22324339500665502 1104 68-69 0.21785307431349565 1104 70-71 0.13774182003326274 1104 72-73 0.18794049980711947 1104 74-75 0.19049698647955893 1104 76-77 0.23120824303651943 1104 78-79 0.17908793272926715 1104 80-81 0.10566579823376543 1104 82-83 0.09463833990248105 1104 84-85 0.0684447507108743 1104 86-87 0.038935920315829264 1104 88-89 -0.005851762378398462 1104 90-91 0.10540990586418175 1104 92-93 0.09681221034683318 1104 94-95 0.060964819200719944 1104 96-97 0.04710047968634257 1104 98-99 0.08676159519512083 1104 100 0.08384213435841303 1105 1 0.006268982011789603 1105 2 0.015296288624579546 1105 3 0.019577063172178555 1105 4 0.015656824267239244 1105 5 0.028390743966092202 1105 6 -0.07446576855473097 1105 7 0.02143359482706586 1105 8 -0.06779503865021042 1105 9 0.09910550123249351 1105 10-11 0.025785104823306426 1105 12-13 0.0645860693856335 1105 14-15 0.09029544857623506 1105 16-17 0.11286333773254142 1105 18-19 0.14935752207594533 1105 20-21 0.07129402220301984 1105 22-23 0.13868827512467874 1105 24-25 0.10816983645366207 1105 26-27 0.15018003327007534 1105 28-29 0.17326291750434564 1105 30-31 0.11315874343451071 1105 32-33 0.19490175575004542 1105 34-35 0.1664969801011651 1105 36-37 0.09639833453070423 1105 38-39 0.1106053103696425 1105 40-41 0.1642063268603451 1105 42-43 0.26429428455428905 1105 44-45 0.21670380915198706 1105 46-47 0.2342496682775419 1105 48-49 0.19430827151729346 1105 50-51 0.17041978479165465 1105 52-53 0.24314553286230733 1105 54-55 0.1822749161321795 1105 56-57 0.14379854458313446 1105 58-59 0.12550996402472947 1105 60-61 0.081053078932527 1105 62-63 0.09623114242342723 1105 64-65 0.11364424626753333 1105 66-67 0.07615456768464668 1105 68-69 0.07379043001613184 1105 70-71 0.12772095570272057 1105 72-73 0.1107843764681462 1105 74-75 0.12080285414496927 1105 76-77 0.17175345193634683 1105 78-79 0.1370762350226329 1105 80-81 0.06751870470341004 1105 82-83 0.10377589056689374 1105 84-85 0.044327209281497915 1105 86-87 0.1093089984886646 1105 88-89 0.11575735636016304 1105 90-91 0.121764035120421 1105 92-93 0.2159629294821599 1105 94-95 0.18430807164201823 1105 96-97 0.18745029926118661 1105 98-99 0.19762430105937057 1105 100 0.21600997250593323 1106 1 0.001885049510566006 1106 2 0.07270678714223067 1106 3 0.16763838341766046 1106 4 0.017222217857849387 1106 5 0.12263159035821758 1106 6 0.04597652779877137 1106 7 0.12584576254424462 1106 8 0.12939223557187063 1106 9 0.1441971411172247 1106 10-11 0.18387615509029587 1106 12-13 0.23614780900711452 1106 14-15 0.2165039573416081 1106 16-17 0.17502607223016042 1106 18-19 0.30674476806280637 1106 20-21 0.3018353909466285 1106 22-23 0.2942452147594494 1106 24-25 0.3092735012186054 1106 26-27 0.34169036818956755 1106 28-29 0.33225334244434634 1106 30-31 0.2891301594305986 1106 32-33 0.30961853162056485 1106 34-35 0.26551539112286093 1106 36-37 0.2008628204421683 1106 38-39 0.2958810604713733 1106 40-41 0.24199393160053972 1106 42-43 0.2527253200393176 1106 44-45 0.25409138731786385 1106 46-47 0.257482683549469 1106 48-49 0.3091388186991679 1106 50-51 0.2988555538275932 1106 52-53 0.2914929422812449 1106 54-55 0.3814776865051357 1106 56-57 0.37334420674046953 1106 58-59 0.39925661109283794 1106 60-61 0.4527904582062092 1106 62-63 0.44413218629596685 1106 64-65 0.3692518432015177 1106 66-67 0.26893797866483027 1106 68-69 0.3971945174788658 1106 70-71 0.29776588291398554 1106 72-73 0.23436701014943395 1106 74-75 0.1886184449668704 1106 76-77 0.30640221415431057 1106 78-79 0.31018675928415007 1106 80-81 0.30973159823174257 1106 82-83 0.29473148653756454 1106 84-85 0.2319639217058196 1106 86-87 0.27371311086481853 1106 88-89 0.29678536735529093 1106 90-91 0.24085768468817292 1106 92-93 0.3478533602352911 1106 94-95 0.38596979714588997 1106 96-97 0.34744058302510794 1106 98-99 0.23991567187910334 1106 100 0.24304103954796474 1107 1 -0.04156222680798649 1107 2 0.12060147926209552 1107 3 0.05057627511170182 1107 4 0.12542877418884046 1107 5 0.15614753387892222 1107 6 0.09163869414238235 1107 7 0.12137509961635828 1107 8 0.10859808166306806 1107 9 0.05308764394455778 1107 10-11 0.08867436201536805 1107 12-13 0.10278425440922945 1107 14-15 0.12447980598197006 1107 16-17 0.20652432548916977 1107 18-19 0.1606999059182712 1107 20-21 0.12302450551969457 1107 22-23 0.02697420296433961 1107 24-25 0.02254584691780792 1107 26-27 0.07869482362161051 1107 28-29 0.10690050717668953 1107 30-31 0.04373589618013085 1107 32-33 0.050998256599037006 1107 34-35 0.11155007262920691 1107 36-37 0.1252543469446863 1107 38-39 0.058466173011510136 1107 40-41 -0.010601329512788027 1107 42-43 -0.05698131725581135 1107 44-45 -0.006076825683543063 1107 46-47 -0.07679536491036743 1107 48-49 -0.09330902356667536 1107 50-51 -0.18474079268606403 1107 52-53 -0.21164782468154186 1107 54-55 -0.22953014857814225 1107 56-57 -0.07448242741228484 1107 58-59 -0.09863230932663924 1107 60-61 -0.03875039340817921 1107 62-63 -0.021102938348711575 1107 64-65 -0.03881498988251053 1107 66-67 -0.03973175662255102 1107 68-69 -0.11480300389646914 1107 70-71 0.010720785091685059 1107 72-73 -0.05186724393043818 1107 74-75 0.031091105544390985 1107 76-77 0.0426110567199629 1107 78-79 0.03765428144508576 1107 80-81 0.02237827557605243 1107 82-83 0.05934430965258031 1107 84-85 -0.003942307401821665 1107 86-87 -0.04524387874451463 1107 88-89 -0.06622693117629552 1107 90-91 0.0401949010932654 1107 92-93 0.035999570232199574 1107 94-95 -0.04591932848959601 1107 96-97 -0.02603853384060173 1107 98-99 0.017583760628617995 1107 100 0.05261741277176313 1108 1 0.06312870135402804 1108 2 0.14288385352467614 1108 3 0.10641138507666525 1108 4 0.1148434632860642 1108 5 0.028231608121949137 1108 6 0.1623757867972131 1108 7 0.11009154920812136 1108 8 0.11713367170648326 1108 9 0.12881326995506726 1108 10-11 0.1634926645796213 1108 12-13 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2214 86-87 -0.2716751083029081 2214 88-89 -0.2654939461890393 2214 90-91 -0.24581221746510096 2214 92-93 -0.24468333971575618 2214 94-95 -0.32135809267735027 2214 96-97 -0.36567148201173794 2214 98-99 -0.3726255354067085 2214 100 -0.2526372917492239 2215 1 -0.12146656678071821 2215 2 -0.2548207345444702 2215 3 -0.39989186126277687 2215 4 -0.2891923773885807 2215 5 -0.2996561133800668 2215 6 -0.3022068463794696 2215 7 -0.3166018198263103 2215 8 -0.28118659509681976 2215 9 -0.40727716549186965 2215 10-11 -0.3573334376276449 2215 12-13 -0.3175389300133844 2215 14-15 -0.44610253116346854 2215 16-17 -0.4467063352852989 2215 18-19 -0.4455746391025528 2215 20-21 -0.4056275568885823 2215 22-23 -0.40037590011198887 2215 24-25 -0.4307049093310553 2215 26-27 -0.35515776655972786 2215 28-29 -0.43145887601755106 2215 30-31 -0.393057571537625 2215 32-33 -0.3855617694647435 2215 34-35 -0.40982109310322556 2215 36-37 -0.4230917444623401 2215 38-39 -0.3484424700845068 2215 40-41 -0.3118581320397098 2215 42-43 -0.41999028271480654 2215 44-45 -0.3532918157007785 2215 46-47 -0.34588896619573006 2215 48-49 -0.3269330683354923 2215 50-51 -0.3909565596045468 2215 52-53 -0.3939575186826971 2215 54-55 -0.3722372549504982 2215 56-57 -0.31625395718374705 2215 58-59 -0.3506412766420226 2215 60-61 -0.31888165444792094 2215 62-63 -0.3243452712058783 2215 64-65 -0.3971007066876169 2215 66-67 -0.361892145425891 2215 68-69 -0.3642031563064876 2215 70-71 -0.4754816761166225 2215 72-73 -0.4064214910370154 2215 74-75 -0.3793396987294031 2215 76-77 -0.3354997969710567 2215 78-79 -0.3941787831750929 2215 80-81 -0.37999299040171053 2215 82-83 -0.4886349117197213 2215 84-85 -0.38849937622859443 2215 86-87 -0.4670676103395195 2215 88-89 -0.40974398262720513 2215 90-91 -0.40544974130475 2215 92-93 -0.4510924521893358 2215 94-95 -0.3983593738970157 2215 96-97 -0.476226122541469 2215 98-99 -0.5243265578637661 2215 100 -0.49284801790720323 2216 1 -0.013735499130493878 2216 2 -0.08040614315894246 2216 3 -0.14994225793476446 2216 4 -0.09499938300595545 2216 5 -0.2086713580460895 2216 6 -0.26789631212238163 2216 7 -0.21035603591153063 2216 8 -0.3157353681669335 2216 9 -0.30310680815983204 2216 10-11 -0.32603459539846114 2216 12-13 -0.3295814438604765 2216 14-15 -0.4472687450600361 2216 16-17 -0.43434791502679815 2216 18-19 -0.374643068620081 2216 20-21 -0.4012886434146523 2216 22-23 -0.356469163189594 2216 24-25 -0.40912062558775375 2216 26-27 -0.4564015171542266 2216 28-29 -0.4026487785316277 2216 30-31 -0.3924440847773383 2216 32-33 -0.48174283619866287 2216 34-35 -0.4702701558123934 2216 36-37 -0.45697423425865225 2216 38-39 -0.3399491036828195 2216 40-41 -0.41172306459543506 2216 42-43 -0.36421927472026994 2216 44-45 -0.36541668774558644 2216 46-47 -0.3632440746729344 2216 48-49 -0.41581185364915996 2216 50-51 -0.3525158980860539 2216 52-53 -0.28783074509072293 2216 54-55 -0.30403812220959736 2216 56-57 -0.42041558781934185 2216 58-59 -0.30008588786333235 2216 60-61 -0.324957507766122 2216 62-63 -0.2969414808525457 2216 64-65 -0.297382164464814 2216 66-67 -0.4366035753910964 2216 68-69 -0.4349360853610591 2216 70-71 -0.4457881096990448 2216 72-73 -0.4238408498168056 2216 74-75 -0.4538587050379306 2216 76-77 -0.46862915104717473 2216 78-79 -0.3263567528044433 2216 80-81 -0.38243559273749383 2216 82-83 -0.37915872294731656 2216 84-85 -0.3511650416522869 2216 86-87 -0.3598664713311024 2216 88-89 -0.3618239470920983 2216 90-91 -0.3996241260071969 2216 92-93 -0.38882616402858616 2216 94-95 -0.3422471520713728 2216 96-97 -0.21798092157001392 2216 98-99 -0.192550539872812 2216 100 -0.2392384380889112 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 32450.0 3 4976.0 4 3273.0 5 2671.0 6 2241.0 7 2246.0 8 2626.0 9 2777.0 10 3049.0 11 3345.0 12 3517.0 13 3741.0 14 3935.0 15 4043.0 16 4068.0 17 4084.0 18 4015.0 19 3968.0 20 3977.0 21 4268.0 22 4755.0 23 6921.0 24 10513.0 25 13425.0 26 13086.0 27 12243.0 28 13609.0 29 16747.0 30 20777.0 31 25302.0 32 32771.0 33 43809.0 34 64734.0 35 103639.0 36 205433.0 37 401141.0 38 522261.0 39 174082.0 40 206.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.71194979274704 17.825941161693194 12.214634660589638 24.247474384970126 2 16.31651463348573 18.82471163977697 39.50825252218814 25.350521204549164 3 19.56615120274914 23.681114460804107 28.32337397826887 28.429360358177885 4 12.771672689506353 15.21310982653773 36.626772363577636 35.38844512037828 5 13.745719883852257 37.78138293875326 34.25780851323934 14.215088664155143 6 33.46999384831412 35.903694993021176 16.85578754757463 13.770523611090082 7 30.45799112415086 31.262414020118147 20.17023820338637 18.10935665234463 8 27.094041633534722 35.675897254151636 18.56753364878308 18.662527463530562 9 26.19500637871332 16.606300206081507 19.210609692840418 37.98808372236475 10-11 25.566906712036403 26.101877782101905 27.04797468027908 21.28324082558261 12-13 25.97350854487095 23.149938428479462 27.33223162555543 23.544321401094155 14-15 22.904797993179567 25.111778967133464 24.241407241611636 27.742015798075332 16-17 23.196650781692682 28.624439009578367 24.825867619858563 23.353042588870387 18-19 23.340265046195373 27.645169945655635 26.34402286259648 22.670542145552513 20-21 24.061120924103513 26.716881150643452 26.20399809347576 23.01799983177727 22-23 23.9501405793867 26.373382664156015 26.20880328642868 23.46767347002861 24-25 23.778163261472432 26.49008497868734 26.092289731090073 23.639462028750156 26-27 23.520766382611495 26.62345745817256 26.277084727020494 23.57869143219545 28-29 23.49673966738944 26.73237236368367 26.28628798698222 23.48459998194467 30-31 23.471842469455915 26.952365358173836 26.028220334611095 23.54757183775915 32-33 23.460809819280975 26.748249150404423 26.374794382589826 23.416146647724773 34-35 23.731743621043933 26.544514962709925 26.29860599248563 23.42513542376051 36-37 23.78828738786046 26.672746688766324 26.057642085939143 23.481323837434076 38-39 23.43786052742174 26.89155026756304 26.23881252307726 23.43177668193796 40-41 23.360730885497187 26.741519291274784 26.39187247073099 23.50587735249704 42-43 23.386071122555638 26.711164994100784 26.26701351660528 23.635750366738296 44-45 23.41468557271625 26.802583338286766 26.320744877119278 23.461986211877704 46-47 23.53913810536162 26.902032345022548 26.21762090969605 23.341208639919785 48-49 23.34251184701231 26.649384516165213 26.345708437315984 23.66239519950649 50-51 23.224248120722415 26.737841057195986 26.425637586000363 23.612273236081233 52-53 23.296679570025137 26.76659408819616 26.394663309269806 23.542063032508892 54-55 23.44350200027536 26.963493117017016 26.210154674688518 23.382850208019104 56-57 23.28748532462751 26.715837401219005 26.324064190016177 23.672613084137307 58-59 23.349592529368618 26.336224397093755 26.583953754943373 23.730229318594255 60-61 23.503140922615877 26.736676173738278 26.245321702031255 23.514861201614597 62-63 23.217534742521348 26.84385682012735 26.394081959909165 23.54452647744214 64-65 23.352656747469847 26.7147345355441 26.350033716670616 23.582575000315437 66-67 23.23574026148812 26.591597948694947 26.33540912544757 23.83725266436937 68-69 23.263840795037854 26.70834499268475 26.361620648912304 23.66619356336509 70-71 23.37460427920988 26.52169731729973 26.351450810339166 23.752247593151225 72-73 23.1602478676357 26.57542335362634 26.540515973140664 23.723812805597294 74-75 23.213155183909354 26.77697545613546 26.382369285654605 23.627500074300578 76-77 23.30377765727715 26.47315287772507 26.364113517572186 23.85895594742559 78-79 23.208095816079425 26.57350186438953 26.482238041284894 23.736164278246154 80-81 23.153463215904424 26.587645501050734 26.511493584144922 23.74739769889992 82-83 23.074337102493512 26.57452790168276 26.619136499786773 23.731998496036958 84-85 23.080007256232776 26.59919603856794 26.454043345056018 23.866753360143264 86-87 23.279265558282418 26.6479895406599 26.480153357298448 23.592591543759234 88-89 22.92377182013424 26.645208682633054 26.4323204342438 23.998699062988905 90-91 23.01166439382385 26.410134486502006 26.557383299988334 24.02081781968581 92-93 23.208758464302456 26.495677850723236 26.52407910407924 23.77148458089507 94-95 23.012621630881362 26.348306321693737 26.668851672146005 23.9702203752789 96-97 23.054478635488383 26.351508205215048 26.72215312737961 23.87186003191696 98-99 22.988185422145467 26.445813871334927 26.684208165348018 23.88179254117159 100 23.10937186343853 26.146264274684 26.617218103377706 24.127145758499765 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1634.0 1 972.0 2 282.5 3 237.0 4 190.5 5 150.5 6 138.0 7 131.5 8 142.0 9 153.5 10 141.5 11 135.5 12 143.5 13 151.0 14 172.5 15 210.5 16 244.5 17 297.5 18 377.0 19 493.5 20 669.0 21 955.0 22 1410.0 23 2159.5 24 3176.0 25 4308.5 26 5974.0 27 8310.0 28 11265.0 29 14567.5 30 17560.0 31 20867.5 32 24358.0 33 27958.5 34 31630.0 35 35479.5 36 39570.5 37 43749.0 38 47057.5 39 49386.0 40 52268.5 41 55546.0 42 57711.5 43 59417.0 44 60993.5 45 61461.5 46 62509.0 47 63543.0 48 65092.0 49 65452.0 50 63566.0 51 62652.5 52 60415.5 53 59084.5 54 59170.5 55 56681.5 56 52768.5 57 47979.0 58 43372.5 59 41414.0 60 38761.0 61 34489.5 62 31285.5 63 28040.5 64 23716.0 65 19223.0 66 16147.5 67 12655.0 68 8981.5 69 6610.0 70 4806.5 71 3482.5 72 2668.0 73 1985.0 74 1458.5 75 1045.5 76 707.5 77 459.5 78 303.0 79 220.5 80 157.0 81 99.5 82 72.0 83 57.5 84 46.0 85 38.5 86 22.5 87 16.5 88 13.5 89 12.5 90 11.5 91 7.0 92 5.0 93 6.0 94 7.0 95 3.5 96 1.5 97 2.5 98 2.5 99 1.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07821937733789651 2 0.08130108633043541 3 0.08270186314522582 4 0.084718981758524 5 0.08281392529040904 6 0.08253376992745097 7 0.08169330383857672 8 0.08124505525784378 9 0.08118902418525217 10-11 0.08124505525784378 12-13 0.0802364959511947 14-15 0.07875167252751686 16-17 0.07746295785790969 18-19 0.07701470927717675 20-21 0.07698669374088095 22-23 0.0778831909023468 24-25 0.07813533072900908 26-27 0.07760303553938872 28-29 0.07379292260315881 30-31 0.07844350162826297 32-33 0.07707074034976837 34-35 0.07510965280906179 36-37 0.07214000596170612 38-39 0.07354078277649653 40-41 0.075810041216457 42-43 0.08046062024156117 44-45 0.08124505525784378 46-47 0.08477501283111563 48-49 0.08819290825920421 50-51 0.08920146756585333 52-53 0.0894536073925156 54-55 0.08934154524733236 56-57 0.08682014698070963 58-59 0.08561547891998987 60-61 0.08348629816150845 62-63 0.08396256227853718 64-65 0.08301003404447971 66-67 0.0823096456370845 68-69 0.0819734592015348 70-71 0.07998435612453242 72-73 0.08032054256008211 74-75 0.07995634058823661 76-77 0.07973221629787014 78-79 0.0802645114874905 80-81 0.08104894650377313 82-83 0.08043260470526535 84-85 0.08009641826971566 86-87 0.08054466685044859 88-89 0.07810731519271327 90-91 0.07552988585349892 92-93 0.07575401014386539 94-95 0.07603416550682347 96-97 0.07541782370831568 98-99 0.07474545083721629 100 0.0740730779661169 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1784724.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.07049619565679 #Duplication Level Percentage of deduplicated Percentage of total 1 78.5793032516186 47.202977370337166 2 12.336209708982102 14.82084476784467 3 3.6867110551106514 6.643876872315306 4 1.6681063893112473 4.008159140522881 5 0.904600031099635 2.716988636338082 6 0.6066407322748877 2.1864725880149454 7 0.4252251421916815 1.7880439700426114 8 0.27935122480053554 1.3424613349306105 9 0.23078105338715085 1.247681915057026 >10 1.1965398685702509 12.90567796802283 >50 0.0647825179936717 2.6610251398744036 >100 0.020909212666729744 2.0674984389048854 >500 6.531871055459233E-4 0.2604794430493772 >1k 1.8662488729883522E-4 0.14781241474525667 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 5.6031072591616406E-5 0.0 3 0.0 0.0 0.0 5.6031072591616406E-5 0.0 4 0.0 0.0 0.0 5.6031072591616406E-5 0.0 5 0.0 0.0 0.0 5.6031072591616406E-5 0.0 6 0.0 0.0 0.0 5.6031072591616406E-5 0.0 7 0.0 0.0 0.0 5.6031072591616406E-5 0.0 8 0.0 0.0 0.0 5.6031072591616406E-5 0.0 9 0.0 0.0 0.0 5.6031072591616406E-5 0.0 10-11 0.0 0.0 0.0 5.6031072591616406E-5 0.0 12-13 0.0 0.0 0.0 5.6031072591616406E-5 0.0 14-15 0.0 0.0 0.0 5.6031072591616406E-5 0.0 16-17 0.0 0.0 0.0 2.2412429036646562E-4 0.0 18-19 0.0 0.0 0.0 2.2412429036646562E-4 0.0 20-21 0.0 0.0 0.0 2.521398266622738E-4 0.0 22-23 0.0 0.0 0.0 3.3618643554969845E-4 0.0 24-25 0.0 0.0 0.0 3.922175081413148E-4 0.0 26-27 0.0 0.0 0.0 4.482485807329312E-4 0.0 28-29 0.0 0.0 0.0 0.001120621451832328 0.0 30-31 0.0 0.0 0.0 0.003922175081413149 0.0 32-33 0.0 0.0 0.0 0.00952528234057479 0.0 34-35 0.0 0.0 0.0 0.017341616967105275 0.0 36-37 0.0 0.0 0.0 0.03261008424832075 0.0 38-39 0.0 0.0 0.0 0.0635112207825972 0.0 40-41 0.0 0.0 0.0 0.10354542214930712 0.0 42-43 0.0 0.0 0.0 0.1440839031693416 0.0 44-45 0.0 0.0 0.0 0.189889305012988 0.0 46-47 0.0 0.0 0.0 0.2496464439319469 0.0 48-49 0.0 0.0 0.0 0.31609929602560394 0.0 50-51 0.0 0.0 0.0 0.3954953258879244 0.0 52-53 0.0 0.0 0.0 0.4770205365087263 0.0 54-55 0.0 0.0 0.0 0.564260916533873 0.0 56-57 0.0 0.0 0.0 0.6660413598965442 0.0 58-59 0.0 0.0 0.0 0.786284041678153 0.0 60-61 0.0 0.0 0.0 0.9170885806432816 0.0 62-63 0.0 0.0 0.0 1.0521794966616687 0.0 64-65 0.0 0.0 0.0 1.1909684634711026 0.0 66-67 0.0 0.0 0.0 1.3440173382551026 0.0 68-69 0.0 0.0 0.0 1.5250537337986154 0.0 70-71 0.0 0.0 0.0 1.7240761036440369 0.0 72-73 0.0 0.0 0.0 1.933072004410766 0.0 74-75 0.0 0.0 0.0 2.146774515275191 0.0 76-77 0.0 0.0 0.0 2.3646513410476913 0.0 78-79 0.0 0.0 0.0 2.623122678912818 0.0 80-81 0.0 0.0 0.0 2.909805661827823 0.0 82-83 0.0 0.0 0.0 3.2021477830745817 0.0 84-85 0.0 0.0 0.0 3.487289911493318 2.8015536295808203E-5 86-87 0.0 0.0 0.0 3.7880927247014107 5.6031072591616406E-5 88 0.0 0.0 0.0 4.029026336845361 5.6031072591616406E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 6755 0.0 29.154385 1 TACATGG 6825 0.0 27.684622 2 ACATGGG 6790 0.0 26.165995 3 TAACCGT 120 1.8387997E-4 23.499622 7 CATGGGG 5065 0.0 21.436197 4 CAGCGTT 505 0.0 21.405596 9 TACCTGG 1410 0.0 20.667492 2 GAGTACA 4730 0.0 20.569534 1 GTATCAA 4555 0.0 20.327925 1 GTACCTG 1625 0.0 19.957722 1 AGTACAT 4665 0.0 19.747843 2 TCAACGC 4690 0.0 19.442142 4 CTACACT 1050 0.0 19.248388 4 ATCAACG 4760 0.0 19.156229 3 TACACTG 1400 0.0 19.135942 5 CAACGCA 4805 0.0 18.976294 5 GTACAAG 1205 0.0 18.72274 1 GGTATCA 3730 0.0 18.52353 1 AACGCAG 4910 0.0 18.378523 6 GTCTAGA 720 0.0 18.27851 1 >>END_MODULE