FastQCFastQC Report
Wed 25 May 2016
SRR1032954_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032954_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1784724
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33800.18938502535966345No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27830.15593447502246846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41650.033.723621
GTATCAA61250.031.5219971
GTACATG66100.030.204071
TACATGG66400.029.1154862
CGTATAC1205.808788E-627.4389233
ATCAACG70050.026.9269123
ACATGGG68550.026.6926963
TCAACGC71250.026.4734064
CAACGCA71800.026.269145
AACGCAG73750.025.510796
TATCAAC74050.025.4723852
TACCTGG13800.023.519082
TATAACG1852.7946044E-722.8834292
CATGGGG49500.022.3312454
ACGCAGA85100.021.9413097
ACCGTCC1303.1456878E-421.7074838
ACACCGT3500.021.5019516
CGCAGAG87850.021.254478
TATGCGA1150.00394219520.4502265
GTACCTG17250.020.4244381
Produced by FastQC (version 0.11.5)