##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032954_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1784724 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.84587981110805 34.0 31.0 34.0 31.0 34.0 2 32.88358872296221 34.0 31.0 34.0 31.0 34.0 3 32.96996342291581 34.0 31.0 34.0 31.0 34.0 4 36.382475945860534 37.0 37.0 37.0 35.0 37.0 5 36.36298217539519 37.0 37.0 37.0 35.0 37.0 6 36.421279704873136 37.0 37.0 37.0 35.0 37.0 7 36.38805888193357 37.0 37.0 37.0 35.0 37.0 8 36.31887003256526 37.0 37.0 37.0 35.0 37.0 9 38.164698295086524 39.0 39.0 39.0 37.0 39.0 10-11 38.175099903402426 39.0 39.0 39.0 37.0 39.0 12-13 38.175161817737646 39.0 39.0 39.0 37.0 39.0 14-15 39.738279140079925 41.0 40.0 41.0 37.0 41.0 16-17 39.74607866538467 41.0 40.0 41.0 37.0 41.0 18-19 39.754023031012075 41.0 40.0 41.0 37.0 41.0 20-21 39.759203383828535 41.0 40.0 41.0 37.0 41.0 22-23 39.731589030012486 41.0 40.0 41.0 37.0 41.0 24-25 39.71056224940103 41.0 40.0 41.0 37.0 41.0 26-27 39.63783867981828 41.0 40.0 41.0 37.0 41.0 28-29 39.57543351240864 41.0 40.0 41.0 37.0 41.0 30-31 39.47318520958983 41.0 39.5 41.0 36.0 41.0 32-33 39.365603308971025 41.0 39.0 41.0 35.5 41.0 34-35 39.27936728592208 41.0 39.0 41.0 35.0 41.0 36-37 39.19788129705209 41.0 39.0 41.0 35.0 41.0 38-39 39.1055602995197 41.0 39.0 41.0 35.0 41.0 40-41 39.008334622048004 41.0 39.0 41.0 35.0 41.0 42-43 38.87014434724921 41.0 39.0 41.0 35.0 41.0 44-45 38.699665326403405 41.0 38.5 41.0 35.0 41.0 46-47 38.47407106084751 41.0 38.0 41.0 35.0 41.0 48-49 38.26029010648145 40.5 38.0 41.0 34.5 41.0 50-51 38.088997234306255 40.0 38.0 41.0 34.0 41.0 52-53 37.92335817751092 40.0 37.0 41.0 34.0 41.0 54-55 37.74551611341585 40.0 37.0 41.0 34.0 41.0 56-57 37.50626791593546 40.0 36.5 41.0 33.0 41.0 58-59 37.30289165159431 39.5 36.0 41.0 33.0 41.0 60-61 37.075262337481874 39.0 35.0 41.0 33.0 41.0 62-63 36.812935221356355 39.0 35.0 41.0 33.0 41.0 64-65 36.50108280047783 38.5 35.0 41.0 32.0 41.0 66-67 36.204490162064275 37.5 35.0 40.0 32.0 41.0 68-69 35.88836509174528 37.0 35.0 40.0 32.0 41.0 70-71 35.556258558746336 36.5 35.0 39.0 31.5 41.0 72-73 35.21191876166847 36.0 35.0 39.0 31.0 41.0 74-75 34.875338147523095 35.5 35.0 38.5 31.0 40.5 76-77 34.54873778802773 35.0 35.0 37.0 31.0 39.5 78-79 34.234058319381596 35.0 34.5 37.0 31.0 39.0 80-81 33.93944497860734 35.0 34.0 36.5 30.5 39.0 82-83 33.67325003754082 35.0 34.0 36.0 30.0 37.5 84-85 33.44009914137985 35.0 34.0 36.0 30.0 37.0 86-87 33.33284166067134 35.0 34.0 35.0 30.0 37.0 88-89 33.182164861345505 35.0 34.0 35.0 30.0 36.0 90-91 33.04223342096593 35.0 34.0 35.0 30.0 36.0 92-93 32.90477351119837 35.0 34.0 35.0 30.0 36.0 94-95 32.82362118736566 35.0 34.0 35.0 30.0 36.0 96-97 32.779841028640845 35.0 34.0 35.0 30.0 35.5 98-99 32.68356759924784 35.0 34.0 35.0 30.0 35.0 100 32.60433321902995 35.0 34.0 35.0 30.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23923201044130593 1101 2 0.25102470164924995 1101 3 0.207617987133915 1101 4 0.14898785068259457 1101 5 0.16281973846240305 1101 6 0.17653797159747597 1101 7 0.14373906431530514 1101 8 0.0374408828496442 1101 9 0.14330672858642401 1101 10-11 0.20462444172441252 1101 12-13 0.24130139299089848 1101 14-15 0.39184213692701775 1101 16-17 0.30679253788459704 1101 18-19 0.33405411931631335 1101 20-21 0.35484846864765984 1101 22-23 0.31426129714733264 1101 24-25 0.30113954629163686 1101 26-27 0.3476899334434762 1101 28-29 0.27829207995090854 1101 30-31 0.33404749286427915 1101 32-33 0.3419144226112536 1101 34-35 0.3402836144453829 1101 36-37 0.3236124407961043 1101 38-39 0.25478577547148973 1101 40-41 0.2941250319639934 1101 42-43 0.3245720083375616 1101 44-45 0.18444100545047348 1101 46-47 0.2535921846314082 1101 48-49 0.2528327932388734 1101 50-51 0.19583181892913615 1101 52-53 0.20152653054437764 1101 54-55 0.22900995822213588 1101 56-57 0.20803337157471447 1101 58-59 0.2191646918158483 1101 60-61 0.2418942759148237 1101 62-63 0.16729441941040335 1101 64-65 0.20156535484193228 1101 66-67 0.2981826989972163 1101 68-69 0.21000234706755094 1101 70-71 0.30192487747058294 1101 72-73 0.34335080396379425 1101 74-75 0.29021851873427806 1101 76-77 0.2612715613808163 1101 78-79 0.253678781041927 1101 80-81 0.19353609996409205 1101 82-83 0.23163265174041214 1101 84-85 0.27410377139220543 1101 86-87 0.20174453189378028 1101 88-89 0.23630657686081946 1101 90-91 0.3257045718436373 1101 92-93 0.31276522499288006 1101 94-95 0.33759660765439037 1101 96-97 0.2916908161862466 1101 98-99 0.32808750005058585 1101 100 0.34607627614040837 1102 1 0.2066323377735486 1102 2 0.2321526885559635 1102 3 0.214721096790214 1102 4 0.17175380812940233 1102 5 0.20041319181756023 1102 6 0.18131833166294342 1102 7 0.16596754222037902 1102 8 0.05763793685946439 1102 9 0.15268610665516746 1102 10-11 0.19126241226450702 1102 12-13 0.19947324241807252 1102 14-15 0.312057194210162 1102 16-17 0.290446547704569 1102 18-19 0.3681703222623014 1102 20-21 0.3347384850142703 1102 22-23 0.33917193544520785 1102 24-25 0.31144756593157297 1102 26-27 0.36341202836982234 1102 28-29 0.336899608592482 1102 30-31 0.27997318844529673 1102 32-33 0.37705517547541234 1102 34-35 0.37403615127025347 1102 36-37 0.377346319683177 1102 38-39 0.3639903581228765 1102 40-41 0.3491715131423945 1102 42-43 0.42258019164361116 1102 44-45 0.3454239841738769 1102 46-47 0.2817587967427073 1102 48-49 0.22153492089844917 1102 50-51 0.2920573508440256 1102 52-53 0.41394027849855064 1102 54-55 0.3786469467655138 1102 56-57 0.37710702132920915 1102 58-59 0.35875978183221946 1102 60-61 0.3324078602028848 1102 62-63 0.4255618498522935 1102 64-65 0.39552771163407385 1102 66-67 0.4680985746109627 1102 68-69 0.38495521122467125 1102 70-71 0.37605941102214047 1102 72-73 0.38996389725348024 1102 74-75 0.43444241398795214 1102 76-77 0.41844472013695366 1102 78-79 0.37696126876697633 1102 80-81 0.3606411736138426 1102 82-83 0.4793328154065364 1102 84-85 0.4872353589535763 1102 86-87 0.5296371669183273 1102 88-89 0.4761929925727628 1102 90-91 0.41095792699912437 1102 92-93 0.4638968125542462 1102 94-95 0.4756051182926839 1102 96-97 0.5020881975283018 1102 98-99 0.46915591248921373 1102 100 0.5425286492991646 1103 1 0.11432377532263871 1103 2 0.18232896506813745 1103 3 0.12644929788998382 1103 4 0.07011987254416852 1103 5 0.0694683371435616 1103 6 0.10111848877213703 1103 7 0.1255050127624031 1103 8 0.10210989286888861 1103 9 0.17120582385862093 1103 10-11 0.14103507526529313 1103 12-13 0.1445447270999196 1103 14-15 0.16619293655109146 1103 16-17 0.21509949350190283 1103 18-19 0.2060846977532691 1103 20-21 0.19548051172283465 1103 22-23 0.19356926458896595 1103 24-25 0.15811088093549586 1103 26-27 0.21970299459134424 1103 28-29 0.1961528686081948 1103 30-31 0.20716816095118418 1103 32-33 0.1777205328988174 1103 34-35 0.2517616815137771 1103 36-37 0.26628960326527107 1103 38-39 0.33501785223128877 1103 40-41 0.2960598085076711 1103 42-43 0.35092347522570577 1103 44-45 0.23465744843153402 1103 46-47 0.3514591895156869 1103 48-49 0.3220172461144699 1103 50-51 0.4248080185580818 1103 52-53 0.4677530043430167 1103 54-55 0.36113901275137295 1103 56-57 0.3806836120597694 1103 58-59 0.33316528065390116 1103 60-61 0.3427665404856768 1103 62-63 0.380031449223857 1103 64-65 0.3847476645013188 1103 66-67 0.39272559739179513 1103 68-69 0.3654841978703942 1103 70-71 0.3845145563481367 1103 72-73 0.35851000880100514 1103 74-75 0.34395191909400324 1103 76-77 0.37972877355408485 1103 78-79 0.3659629969680722 1103 80-81 0.36556921760441696 1103 82-83 0.37214397015908673 1103 84-85 0.4056515412002426 1103 86-87 0.3432874418594096 1103 88-89 0.32376895486655854 1103 90-91 0.32828989871946135 1103 92-93 0.32137505293131596 1103 94-95 0.34518673651735554 1103 96-97 0.32915386917009215 1103 98-99 0.3383307750186759 1103 100 0.3263910216479431 1104 1 0.10031696486639419 1104 2 0.11332407363967434 1104 3 0.1472477330154831 1104 4 0.07979033780976863 1104 5 0.06364728618590476 1104 6 0.08411954931895593 1104 7 0.1159687598763881 1104 8 0.0540385456874688 1104 9 0.09510680680737238 1104 10-11 0.13994643052934208 1104 12-13 0.17867263359006103 1104 14-15 0.2216891576804798 1104 16-17 0.16720271208813386 1104 18-19 0.206047034591073 1104 20-21 0.16310439064593396 1104 22-23 0.19640207243151053 1104 24-25 0.14676963594678938 1104 26-27 0.17278889290559363 1104 28-29 0.1953084365985731 1104 30-31 0.2073747105153103 1104 32-33 0.19234893498834538 1104 34-35 0.1806440660343398 1104 36-37 0.1536677808703999 1104 38-39 0.15263114959928714 1104 40-41 0.1888126090328015 1104 42-43 0.1730429009282446 1104 44-45 0.22765807854221265 1104 46-47 0.10405316508364848 1104 48-49 0.12614070476450934 1104 50-51 0.10094532649090127 1104 52-53 0.07904286601758059 1104 54-55 0.06033887979442909 1104 56-57 0.030154205499684394 1104 58-59 0.06487667680939069 1104 60-61 0.07414210677821842 1104 62-63 0.041472352135407675 1104 64-65 -0.0066138408773355195 1104 66-67 0.05148030977078122 1104 68-69 0.15897438930686292 1104 70-71 0.09045149625805493 1104 72-73 0.11209144672076121 1104 74-75 0.059998578371519784 1104 76-77 0.12547881450529275 1104 78-79 0.17041301914748885 1104 80-81 0.2045461964448947 1104 82-83 0.2036515368677243 1104 84-85 0.23961618087138703 1104 86-87 0.24095558396551553 1104 88-89 0.2196980154038144 1104 90-91 0.26474392395497404 1104 92-93 0.19494430113872596 1104 94-95 0.21229705132160603 1104 96-97 0.22036277895905698 1104 98-99 0.25317630822741677 1104 100 0.2262138852200195 1105 1 0.06799862990837369 1105 2 0.12405381215146605 1105 3 0.07678102188384628 1105 4 0.0683081152455074 1105 5 0.0725060757126954 1105 6 0.09061570994009571 1105 7 0.06853233622786803 1105 8 0.04202445371339536 1105 9 0.058350526130823255 1105 10-11 0.07675829241431842 1105 12-13 0.1159676244405361 1105 14-15 0.12774708185061456 1105 16-17 0.13895890725513738 1105 18-19 0.21436882413496505 1105 20-21 0.12606208051988688 1105 22-23 0.16874571821674778 1105 24-25 0.19140861462071257 1105 26-27 0.1743618411039094 1105 28-29 0.09076702432282246 1105 30-31 0.19044809481233926 1105 32-33 0.22523120543795017 1105 34-35 0.18419869808673894 1105 36-37 0.27895381031988364 1105 38-39 0.29534616336632524 1105 40-41 0.25248686894763495 1105 42-43 0.23868131523911984 1105 44-45 0.20168540739485508 1105 46-47 0.21995879674270924 1105 48-49 0.20320533288347065 1105 50-51 0.1824183995331623 1105 52-53 0.16758971670079603 1105 54-55 0.20126717148461637 1105 56-57 0.25664634717190893 1105 58-59 0.1925343136674229 1105 60-61 0.2100535530867731 1105 62-63 0.18422926558263697 1105 64-65 0.17467752436816397 1105 66-67 0.22033003528512296 1105 68-69 0.12172899399620718 1105 70-71 0.13474330615322572 1105 72-73 0.05871595717857758 1105 74-75 0.1019794926396429 1105 76-77 0.16579827818939208 1105 78-79 0.1734661376808333 1105 80-81 0.1574898627524206 1105 82-83 0.13618450079979283 1105 84-85 0.10907131400975345 1105 86-87 0.12041319688087526 1105 88-89 0.12133082028811515 1105 90-91 0.0737459419803912 1105 92-93 0.16889381629957256 1105 94-95 0.1176448186672232 1105 96-97 0.08000355333354747 1105 98-99 0.10042332821954858 1105 100 -0.010627905007950744 1106 1 0.15174926312461423 1106 2 0.19142718966460848 1106 3 0.13078170285082535 1106 4 0.08958085912718161 1106 5 0.09983610386486674 1106 6 0.10621234496671406 1106 7 0.08780287111911633 1106 8 -0.043839668462048564 1106 9 0.05235469497741008 1106 10-11 0.08859175446701784 1106 12-13 0.09805860827172097 1106 14-15 0.16022629990121828 1106 16-17 0.16694706507337997 1106 18-19 0.1481518448048007 1106 20-21 0.14419199573119812 1106 22-23 0.121755675282607 1106 24-25 0.17730595469727461 1106 26-27 0.18290382437869823 1106 28-29 0.14155844081717106 1106 30-31 0.09830371320508391 1106 32-33 0.13305443637710823 1106 34-35 0.09392572998422821 1106 36-37 0.19329813047401245 1106 38-39 0.1580760935256933 1106 40-41 0.14845961365976024 1106 42-43 0.07929933428958691 1106 44-45 0.12822235815761474 1106 46-47 0.19401644641011018 1106 48-49 0.1319961182376943 1106 50-51 0.06166385490906379 1106 52-53 4.588298658845247E-4 1106 54-55 0.060292475220791175 1106 56-57 0.044832815859891184 1106 58-59 -0.0332651996903266 1106 60-61 -0.021677431740947384 1106 62-63 -0.03449151304496212 1106 64-65 0.013189943710941066 1106 66-67 0.013510400183761817 1106 68-69 0.05603976407019928 1106 70-71 0.08395105921134416 1106 72-73 0.09967653583441916 1106 74-75 0.14640220704043827 1106 76-77 0.120278127380125 1106 78-79 0.1228453781535137 1106 80-81 0.13771981367297315 1106 82-83 0.09596592701037565 1106 84-85 0.1376358024125608 1106 86-87 0.18913812774611927 1106 88-89 0.14917614113151956 1106 90-91 0.09271668531397381 1106 92-93 0.04159659082475997 1106 94-95 0.165317904693282 1106 96-97 0.12209603197028684 1106 98-99 0.07283336999105927 1106 100 0.11657506467241063 1107 1 0.08318707385453195 1107 2 0.10495268855596152 1107 3 0.04858226815681377 1107 4 0.042701024326071035 1107 5 0.0877676386722257 1107 6 0.04682635769331256 1107 7 0.00636898835197286 1107 8 0.05012441552901947 1107 9 0.07139008351705911 1107 10-11 0.07033153735488895 1107 12-13 0.05351951862920856 1107 14-15 0.1010671725181922 1107 16-17 0.12996729969299992 1107 18-19 0.10494906412781546 1107 20-21 0.08556755225939128 1107 22-23 0.07730917333385179 1107 24-25 0.11045002435528772 1107 26-27 0.08643907103071058 1107 28-29 0.08197365053030126 1107 30-31 0.11588294983357628 1107 32-33 0.1646813504573288 1107 34-35 0.15958683818276853 1107 36-37 0.15822903841058888 1107 38-39 0.18765485559215023 1107 40-41 0.1025733931134667 1107 42-43 0.16079494218590895 1107 44-45 0.1753142227855875 1107 46-47 0.06750037302614231 1107 48-49 0.17608589703728228 1107 50-51 0.18891461765529982 1107 52-53 0.17855025680658088 1107 54-55 0.2411779662882907 1107 56-57 0.19038467859602548 1107 58-59 0.19487127857841813 1107 60-61 0.17763461363744426 1107 62-63 0.18581188600558818 1107 64-65 0.13028331539401705 1107 66-67 0.07960558834921727 1107 68-69 0.10943200081252513 1107 70-71 0.11810669952539143 1107 72-73 0.1643696817798741 1107 74-75 0.14604588470379554 1107 76-77 0.10296142295691624 1107 78-79 0.11564037216538026 1107 80-81 0.13122063854515176 1107 82-83 0.15424286602113568 1107 84-85 0.12267266914880537 1107 86-87 0.13159146915982944 1107 88-89 0.19951779372966882 1107 90-91 0.1981347888935474 1107 92-93 0.1770608038774597 1107 94-95 0.19176231279738687 1107 96-97 0.2337415597842636 1107 98-99 0.21053812507055625 1107 100 0.18167022558260726 1108 1 0.023006586700581977 1108 2 0.013035664235502509 1108 3 0.04725328563142028 1108 4 0.07491546764298818 1108 5 0.03847270540840242 1108 6 0.05743106413790855 1108 7 0.04636181975971709 1108 8 0.08003622012126499 1108 9 0.08060141423742806 1108 10-11 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46-47 -0.29075204275552124 2214 48-49 -0.3085512804524626 2214 50-51 -0.23444866395172426 2214 52-53 -0.2724898276448968 2214 54-55 -0.2811506730479323 2214 56-57 -0.3729415473423856 2214 58-59 -0.24399332205970836 2214 60-61 -0.24067744053690632 2214 62-63 -0.27098900251180424 2214 64-65 -0.27083092458335756 2214 66-67 -0.19520261548231588 2214 68-69 -0.2834519293349942 2214 70-71 -0.3444062692609151 2214 72-73 -0.3339526032289868 2214 74-75 -0.2661028417212776 2214 76-77 -0.25418255551438307 2214 78-79 -0.2538963703452879 2214 80-81 -0.22792357389339202 2214 82-83 -0.3943005715346075 2214 84-85 -0.39121256642435753 2214 86-87 -0.4211044220170237 2214 88-89 -0.34311913351279344 2214 90-91 -0.3483798665036133 2214 92-93 -0.4038537824923836 2214 94-95 -0.46153409045610516 2214 96-97 -0.41704277706159587 2214 98-99 -0.4585903724898799 2214 100 -0.4116256125213553 2215 1 -0.22193300240617475 2215 2 -0.24549660541066487 2215 3 -0.25095284415971975 2215 4 -0.11353502370628377 2215 5 -0.18480426646228665 2215 6 -0.16126343983898295 2215 7 -0.18461320232390932 2215 8 -0.10411507982860257 2215 9 -0.16080708975048452 2215 10-11 -0.21285337720917852 2215 12-13 -0.23329569211337997 2215 14-15 -0.28901417934567775 2215 16-17 -0.29458092341224784 2215 18-19 -0.2495511154783827 2215 20-21 -0.26078038533233183 2215 22-23 -0.2626581030657107 2215 24-25 -0.33918760736753484 2215 26-27 -0.3164105646982165 2215 28-29 -0.3495237546937844 2215 30-31 -0.3386832942247935 2215 32-33 -0.32393198755411845 2215 34-35 -0.3373235406424513 2215 36-37 -0.3196749896878046 2215 38-39 -0.31352697178725464 2215 40-41 -0.30956738287815 2215 42-43 -0.32329528974278077 2215 44-45 -0.28427229310468505 2215 46-47 -0.3080257988927215 2215 48-49 -0.30346893019269316 2215 50-51 -0.35655394568999554 2215 52-53 -0.3402217240688401 2215 54-55 -0.409594131540004 2215 56-57 -0.41192507109697374 2215 58-59 -0.4447144158571348 2215 60-61 -0.42999034262125235 2215 62-63 -0.42640811163678904 2215 64-65 -0.39106278900776914 2215 66-67 -0.3662986012555294 2215 68-69 -0.42876982086774973 2215 70-71 -0.45400066599968625 2215 72-73 -0.4647708909365065 2215 74-75 -0.565135506422827 2215 76-77 -0.6149496315960334 2215 78-79 -0.48958995074522704 2215 80-81 -0.5100414964760205 2215 82-83 -0.46156217817498657 2215 84-85 -0.4688527026638809 2215 86-87 -0.5362917162652394 2215 88-89 -0.49376644260053837 2215 90-91 -0.5730115210111464 2215 92-93 -0.6158459345574343 2215 94-95 -0.5629953951861282 2215 96-97 -0.5058311862971152 2215 98-99 -0.4494951786532795 2215 100 -0.595974046464633 2216 1 -0.3130805106289003 2216 2 -0.33386444264730386 2216 3 -0.28595191476654946 2216 4 -0.14351471668365434 2216 5 -0.22702773272357035 2216 6 -0.19796705242951163 2216 7 -0.24157671338805642 2216 8 -0.2152338510399261 2216 9 -0.29723829851138817 2216 10-11 -0.27848184334391846 2216 12-13 -0.2910080628164664 2216 14-15 -0.37321840062327993 2216 16-17 -0.41289376500787256 2216 18-19 -0.38610907950520357 2216 20-21 -0.37380386712712976 2216 22-23 -0.44682357781108095 2216 24-25 -0.3400515503158772 2216 26-27 -0.43467988189530615 2216 28-29 -0.4350080732566042 2216 30-31 -0.4788926171291479 2216 32-33 -0.6354743962445042 2216 34-35 -0.6302301981518994 2216 36-37 -0.5920081330700455 2216 38-39 -0.5929872081585614 2216 40-41 -0.5461326336964945 2216 42-43 -0.6015278980912555 2216 44-45 -0.5939482795922686 2216 46-47 -0.6171928008099812 2216 48-49 -0.6143060036426817 2216 50-51 -0.6249425131940427 2216 52-53 -0.6058984706516881 2216 54-55 -0.5783790219632436 2216 56-57 -0.5309276489631074 2216 58-59 -0.6152841338713841 2216 60-61 -0.6760162050588505 2216 62-63 -0.5992276810790358 2216 64-65 -0.6230793583863203 2216 66-67 -0.6674246068982157 2216 68-69 -0.6851047479867489 2216 70-71 -0.6699692769452312 2216 72-73 -0.6622193794290467 2216 74-75 -0.6846118348223769 2216 76-77 -0.6728440630037653 2216 78-79 -0.6044674192004038 2216 80-81 -0.7324507366512876 2216 82-83 -0.6744522287810923 2216 84-85 -0.7320762657914912 2216 86-87 -0.6438274149987393 2216 88-89 -0.723757109398683 2216 90-91 -0.7086292925793956 2216 92-93 -0.7280851045089705 2216 94-95 -0.7965772066563304 2216 96-97 -0.6644691104254719 2216 98-99 -0.6178058822082733 2216 100 -0.6777340291508693 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 5.0 4 6.0 5 16.0 6 29.0 7 101.0 8 208.0 9 373.0 10 569.0 11 908.0 12 1400.0 13 2239.0 14 2516.0 15 3423.0 16 4514.0 17 6984.0 18 8319.0 19 4781.0 20 3330.0 21 3375.0 22 3518.0 23 3601.0 24 4217.0 25 5069.0 26 6167.0 27 7926.0 28 10428.0 29 13298.0 30 17001.0 31 21701.0 32 28070.0 33 37274.0 34 52975.0 35 84699.0 36 173683.0 37 401696.0 38 618114.0 39 251410.0 40 776.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.57575105576726 17.88447606096415 12.463852893533499 24.07591998973509 2 16.555126978185886 18.830727129694587 39.306702018696534 25.307443873422997 3 19.665852908266437 23.675046491808533 28.714315891037433 27.9447847088876 4 12.790111889593152 15.292735834201443 36.59703663771969 35.32011563848572 5 13.49289996296315 37.33476998210913 34.47077366674343 14.701556388184292 6 33.40077795720675 35.89484690201124 16.581291597232884 14.12308354354913 7 30.176191583482986 31.36630423515371 20.18675355310822 18.27075062825509 8 27.265172459650742 35.12858511205991 18.436789297283525 19.169453131005813 9 26.44492233419884 15.838534026485155 19.427936827407212 38.28860681190879 10-11 25.765440482674073 25.499488996617963 27.065473428944752 21.669597091763208 12-13 26.45910679646466 22.633777137279644 27.634527954493755 23.272588111761944 14-15 23.094553513871507 24.448996046717706 25.030235843783856 27.42621459562693 16-17 22.871771769752634 28.19374312218584 25.45225480242323 23.482230305638293 18-19 22.86870048595831 26.80755143240876 27.833285986997407 22.490462094635518 20-21 23.555098715543693 25.795809324018727 27.90075664360428 22.748335316833305 22-23 23.41442081762474 25.531757529268624 27.915830983989913 23.137990669116725 24-25 23.283396349895447 25.598554615972514 27.875947769841254 23.242101264290792 26-27 23.026759273008466 25.813971080597213 27.997215085606697 23.16205456078762 28-29 23.01117427845451 25.9457146867669 27.95029333076318 23.092817704015406 30-31 22.984926332447824 26.177331694377514 27.693132328683678 23.14460964449098 32-33 23.007458458420874 25.91880149090711 28.04834856426497 23.025391486407045 34-35 23.28995759572348 25.745353520294756 27.90880670577171 23.05588217821005 36-37 23.375745463320218 25.90999144358469 27.61387557408278 23.100387519012312 38-39 23.037250713169005 26.209752687732035 27.726592669368177 23.026403929730783 40-41 22.981397450516724 26.002475705458327 27.817264233344208 23.19886261068074 42-43 23.007631614582298 26.0098971844526 27.656269230780012 23.32620197018509 44-45 23.15118032323423 26.144872027876314 27.500151452938525 23.203796195950936 46-47 23.312711699915965 26.31788281571687 27.210149927577454 23.159255556789713 48-49 23.216337740734204 26.220384254749472 27.133072728144768 23.43020527637155 50-51 23.12194955028437 26.36079403060596 27.04068967896528 23.47656674014439 52-53 23.21670727407914 26.434261568204327 26.95405701566791 23.39497414204863 54-55 23.2621595816415 26.62118405507504 26.893513132045243 23.223143231238215 56-57 23.129585558865383 26.341951655358354 27.010438570267546 23.518024215508717 58-59 23.160961908989524 25.981480697352332 27.256845470858078 23.600711922800066 60-61 23.30051892658892 26.342345727653978 27.017132569548924 23.340002776208177 62-63 23.0171978900063 26.330867224684155 27.191925468799464 23.460009416510076 64-65 23.156801836026187 26.30941815092978 27.087073407428825 23.446706605615212 66-67 23.068132663649955 26.139531938832 27.093937213821295 23.69839818369675 68-69 23.094228067885044 26.363586954390122 27.05498276203086 23.487202215693976 70-71 23.297495859303734 26.12272261705451 26.966858741183508 23.61292278245824 72-73 22.97273976256273 26.312247720095655 27.04356527956143 23.671447237780182 74-75 23.11875673773648 26.434143318518714 26.87866583292431 23.568434110820498 76-77 23.152746069629274 26.149568826767844 26.850853927793295 23.846831175809584 78-79 23.04743563919005 26.288413863548048 26.937814176817866 23.726336320444037 80-81 23.06618016617742 26.343362736654903 26.877844635486035 23.712612461681644 82-83 23.013491468271923 26.221248649298307 27.013837740979614 23.75142214145016 84-85 22.960665066419235 26.368895134485783 26.796608327113887 23.873831471981102 86-87 23.12721779604653 26.36844284592856 26.804339750245276 23.699999607779635 88-89 22.758753425966212 26.417403782283504 26.746223697838673 24.077619093911608 90-91 22.788062774010147 26.234514723462148 26.826932747695086 24.15048975483262 92-93 22.977660347291543 26.312793239634864 26.76838891094368 23.941157502129915 94-95 22.79982630133916 26.11377262284978 26.895584692105118 24.190816383705947 96-97 22.796863592379072 26.13666480456005 26.935194767573794 24.131276835487085 98-99 22.67121489687338 26.20473143318839 26.8924570108479 24.23159665909033 100 22.87726281486661 25.814859888699875 26.85008998590258 24.45778731053093 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 19.0 1 28.5 2 125.0 3 241.0 4 170.5 5 96.0 6 236.0 7 539.5 8 963.0 9 1151.5 10 918.5 11 694.0 12 726.0 13 817.5 14 917.0 15 1007.0 16 1100.0 17 1225.5 18 1335.0 19 1444.0 20 1633.0 21 1884.5 22 2285.0 23 3001.5 24 3947.0 25 5021.5 26 6586.5 27 8699.0 28 11391.5 29 14739.5 30 17750.0 31 20815.5 32 24231.5 33 27592.5 34 31369.5 35 35397.0 36 39135.5 37 42931.0 38 46235.0 39 48802.5 40 51691.5 41 55063.5 42 57388.5 43 59067.5 44 60433.0 45 61334.0 46 62498.5 47 63274.5 48 65161.5 49 65133.5 50 62838.5 51 62233.0 52 60152.0 53 58620.5 54 58605.0 55 56372.0 56 52628.0 57 47800.0 58 43276.5 59 41437.0 60 39063.5 61 34768.5 62 31218.0 63 27891.5 64 23363.0 65 18890.0 66 15795.0 67 12050.0 68 8442.0 69 6196.5 70 4388.0 71 3098.0 72 2361.5 73 1692.0 74 1192.0 75 803.5 76 488.5 77 342.0 78 215.0 79 119.5 80 68.5 81 32.0 82 19.5 83 6.5 84 2.5 85 4.0 86 4.0 87 1.5 88 2.0 89 1.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.6809321777484922E-4 2 4.4824858073293125E-4 3 5.6031072591616406E-5 4 0.011038121300548431 5 8.404660888742461E-4 6 0.0028015536295808202 7 0.0010645903792407118 8 0.1455126955204278 9 0.0014568078873820267 10-11 0.0 12-13 1.1206214518323281E-4 14-15 2.521398266622738E-4 16-17 0.0 18-19 1.6809321777484922E-4 20-21 0.0 22-23 3.0817089925389024E-4 24-25 4.4824858073293125E-4 26-27 0.006107386912486188 28-29 0.007536179263572407 30-31 0.014259907974566374 32-33 0.017201539285626236 34-35 0.02633460411805971 36-37 0.038241207043778196 38-39 0.043984391984418884 40-41 0.05547076186570025 42-43 0.07214000596170612 44-45 0.11161389660249987 46-47 0.11934618462014294 48-49 0.1164605843816747 50-51 0.11752517476091541 52-53 0.11225825393730347 54-55 0.11979443320087589 56-57 0.101808458898967 58-59 0.09331975140133712 60-61 0.09617733610350956 62-63 0.09444037285316945 64-65 0.0 66-67 0.0 68-69 5.6031072591616406E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 2.8015536295808203E-5 78-79 2.8015536295808203E-5 80-81 2.80155362958082E-4 82-83 1.9610875407065744E-4 84-85 0.0 86-87 7.284039436910133E-4 88-89 4.7626411702873946E-4 90-91 2.521398266622738E-4 92-93 0.001540854496269451 94-95 0.0013447457421987938 96-97 3.922175081413149E-4 98-99 2.8015536295808203E-5 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1784724.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.60372680000101 #Duplication Level Percentage of deduplicated Percentage of total 1 76.84389673692418 44.2649483388127 2 13.38887304495547 15.424979700830251 3 3.957456512318626 6.838927312754609 4 1.7689376764684457 4.075896105660677 5 1.0058032921153057 2.8969009026775847 6 0.6231344745078136 2.153692081752617 7 0.43465780467200854 1.7526536602270208 8 0.31421012277573973 1.447973925613479 9 0.2422625588318219 1.2559703627535697 >10 1.3176274380487891 13.617633527763065 >50 0.07711248289757841 3.0142756807524376 >100 0.02456600436477165 2.3415364555576854 >500 0.0010720241542487106 0.39768665708798095 >1k 3.898269651813492E-4 0.5169252877563704 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3380 0.18938502535966345 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2783 0.15593447502246846 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.6031072591616406E-5 0.0 12-13 0.0 0.0 0.0 5.6031072591616406E-5 0.0 14-15 0.0 0.0 0.0 5.6031072591616406E-5 0.0 16-17 0.0 0.0 0.0 1.1206214518323281E-4 0.0 18-19 0.0 0.0 0.0 1.1206214518323281E-4 0.0 20-21 0.0 0.0 0.0 1.9610875407065744E-4 0.0 22-23 0.0 0.0 0.0 2.80155362958082E-4 0.0 24-25 0.0 0.0 0.0 3.3618643554969845E-4 0.0 26-27 0.0 0.0 0.0 3.922175081413149E-4 0.0 28-29 0.0 0.0 0.0 0.0010645903792407118 0.0 30-31 0.0 0.0 0.0 0.0038661440088215322 0.0 32-33 0.0 0.0 0.0 0.009581313413166405 0.0 34-35 0.0 0.0 0.0 0.017509710184880126 0.0 36-37 0.0 0.0 0.0 0.032946270683870446 0.0 38-39 0.0 0.0 0.0 0.0638754227544427 0.0 40-41 0.0 0.0 0.0 0.10404970180263166 0.0 42-43 0.0 0.0 0.0 0.14464421389525775 0.0 44-45 0.0 0.0 0.0 0.1905896934203832 0.0 46-47 0.0 0.0 0.0 0.25040286341193374 0.0 48-49 0.0 0.0 0.0 0.31679968443299916 0.0 50-51 0.0 0.0 0.0 0.3964758696582777 0.0 52-53 0.0 0.0 0.0 0.47825322010574184 0.0 54-55 0.0 0.0 0.0 0.5658297865664383 0.0 56-57 0.0 0.0 0.0 0.6684787115542795 0.0 58-59 0.0 0.0 0.0 0.789505828352171 0.0 60-61 0.0 0.0 0.0 0.9210387712609904 0.0 62-63 0.0 0.0 0.0 1.0569141222956602 0.0 64-65 0.0 0.0 0.0 1.1968517260932223 0.0 66-67 0.0 0.0 0.0 1.3504328960668428 0.0 68-69 0.0 0.0 0.0 1.5333743480784703 0.0 70-71 0.0 0.0 0.0 1.7340496345653444 0.0 72-73 0.0 0.0 0.0 1.9438299703483564 0.0 74-75 0.0 0.0 0.0 2.158513024983135 0.0 76-77 0.0 0.0 0.0 2.3783509382963417 0.0 78-79 0.0 0.0 0.0 2.6389794724562456 0.0 80-81 0.0 0.0 0.0 2.9275955273756615 0.0 82-83 0.0 0.0 0.0 3.222403015816451 0.0 84-85 0.0 0.0 0.0 3.509842418211443 0.0 86-87 0.0 0.0 0.0 3.813726940412075 0.0 88 0.0 0.0 0.0 4.056145375979703 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4165 0.0 33.72362 1 GTATCAA 6125 0.0 31.521997 1 GTACATG 6610 0.0 30.20407 1 TACATGG 6640 0.0 29.115486 2 CGTATAC 120 5.808788E-6 27.438923 3 ATCAACG 7005 0.0 26.926912 3 ACATGGG 6855 0.0 26.692696 3 TCAACGC 7125 0.0 26.473406 4 CAACGCA 7180 0.0 26.26914 5 AACGCAG 7375 0.0 25.51079 6 TATCAAC 7405 0.0 25.472385 2 TACCTGG 1380 0.0 23.51908 2 TATAACG 185 2.7946044E-7 22.883429 2 CATGGGG 4950 0.0 22.331245 4 ACGCAGA 8510 0.0 21.941309 7 ACCGTCC 130 3.1456878E-4 21.707483 8 ACACCGT 350 0.0 21.501951 6 CGCAGAG 8785 0.0 21.25447 8 TATGCGA 115 0.003942195 20.450226 5 GTACCTG 1725 0.0 20.424438 1 >>END_MODULE