FastQCFastQC Report
Wed 25 May 2016
SRR1032953_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032953_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3299063
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29250.040.4952551
GTATCAA47550.032.5218241
TCAACGC49700.030.075084
CAACGCA51600.028.8748195
TAGACCG1154.1986277E-628.6094765
ATCAACG51650.028.5751673
GTACATG117500.028.2820591
AACGCAG52700.028.1825056
TACATGG116650.027.4001942
TATCAAC59100.025.291182
ACATGGG121000.025.1332343
ACGCAGA65100.022.5252787
GAGTACA82450.021.4921651
CGCAGAG70150.020.7027158
CATGGGG83700.020.0483574
AGTACAT86900.019.0934492
CATGGGA41000.017.4259224
TATACTG17650.017.0429575
GCAGAGT81500.016.8968819
GTATAGA13800.016.6896211
Produced by FastQC (version 0.11.5)