##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1032953_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3299063
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.315684180629468	33.0	31.0	34.0	28.0	34.0
2	31.329357153834287	33.0	31.0	34.0	28.0	34.0
3	31.50484364802976	34.0	31.0	34.0	28.0	34.0
4	34.837895487294425	37.0	35.0	37.0	32.0	37.0
5	34.92965548096535	37.0	35.0	37.0	32.0	37.0
6	34.97446638636485	37.0	35.0	37.0	32.0	37.0
7	34.91898275358791	37.0	35.0	37.0	32.0	37.0
8	34.9325869193768	37.0	35.0	37.0	32.0	37.0
9	36.54733177268818	39.0	38.0	39.0	33.0	39.0
10-11	36.533494055736426	39.0	38.0	39.0	33.0	39.0
12-13	36.46996874567112	39.0	38.0	39.0	33.0	39.0
14-15	37.89372679454742	41.0	39.0	41.0	33.0	41.0
16-17	37.899443872396496	41.0	39.0	41.0	33.0	41.0
18-19	37.89150737648842	41.0	39.0	41.0	33.0	41.0
20-21	37.92979491449542	41.0	39.0	41.0	33.0	41.0
22-23	37.90761407102562	41.0	39.0	41.0	33.0	41.0
24-25	37.93483816465463	41.0	39.0	41.0	33.0	41.0
26-27	37.91033287330372	41.0	39.0	41.0	33.0	41.0
28-29	37.884992647912455	41.0	39.0	41.0	33.0	41.0
30-31	37.82948931257148	41.0	38.0	41.0	32.0	41.0
32-33	37.78449699202471	41.0	38.0	41.0	32.0	41.0
34-35	37.70885672689488	40.5	38.0	41.0	32.0	41.0
36-37	37.609749798654946	40.0	38.0	41.0	32.0	41.0
38-39	37.50788936131259	40.0	38.0	41.0	31.0	41.0
40-41	37.41697794191866	40.0	38.0	41.0	31.0	41.0
42-43	37.28008043495987	40.0	38.0	41.0	31.0	41.0
44-45	37.12542455236532	40.0	37.5	41.0	31.0	41.0
46-47	36.98724425692993	40.0	37.0	41.0	30.0	41.0
48-49	36.83738897984064	40.0	37.0	41.0	30.0	41.0
50-51	36.68268656888334	40.0	36.5	41.0	30.0	41.0
52-53	36.5175126998181	40.0	36.0	41.0	30.0	41.0
54-55	36.3344473870308	40.0	35.5	41.0	30.0	41.0
56-57	36.13256733805932	39.0	35.0	41.0	29.5	41.0
58-59	35.90860253350724	39.0	35.0	41.0	29.0	41.0
60-61	35.65344857009399	39.0	35.0	41.0	29.0	41.0
62-63	35.38862943811622	38.0	35.0	40.0	28.5	41.0
64-65	35.09876819569678	38.0	35.0	40.0	28.0	41.0
66-67	34.7959346335611	37.0	34.0	40.0	28.0	41.0
68-69	34.481691165036864	37.0	34.0	40.0	28.0	41.0
70-71	34.14599084649187	36.0	34.0	39.0	27.0	41.0
72-73	33.85803878252704	36.0	34.0	39.0	27.0	41.0
74-75	33.584593413341906	35.0	34.0	38.5	27.0	40.0
76-77	33.271625458501404	35.0	34.0	37.5	26.5	39.5
78-79	32.91750263635463	35.0	33.5	37.0	26.0	39.0
80-81	32.60663012497791	35.0	33.0	36.5	26.0	39.0
82-83	32.30882192913563	35.0	33.0	36.0	26.0	38.0
84-85	32.158379667196414	35.0	33.0	36.0	26.0	37.0
86-87	31.945386159645935	35.0	33.0	35.5	25.5	37.0
88-89	31.724019668615	35.0	33.0	35.0	25.0	36.5
90-91	31.538889830233614	35.0	33.0	35.0	25.0	36.0
92-93	31.357286447697422	35.0	33.0	35.0	24.0	36.0
94-95	31.176969794150644	35.0	33.0	35.0	24.0	36.0
96-97	31.00426712069457	35.0	33.0	35.0	23.0	35.5
98-99	30.81833023497884	35.0	33.0	35.0	20.0	35.0
100	30.684339159331	35.0	32.0	35.0	19.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.38719273037521873
1101	2	0.4868318683083146
1101	3	0.4648978924457623
1101	4	0.4674768503339024
1101	5	0.3619971331368106
1101	6	0.3777872063605585
1101	7	0.3785116123938721
1101	8	0.4158951866099301
1101	9	0.46230305086096735
1101	10-11	0.4148715906026226
1101	12-13	0.32328635978900166
1101	14-15	0.4237125246668896
1101	16-17	0.381052569677081
1101	18-19	0.402999647348139
1101	20-21	0.3996545509578482
1101	22-23	0.40703783475102284
1101	24-25	0.39396871367979003
1101	26-27	0.39938261298840416
1101	28-29	0.46396508223056543
1101	30-31	0.4810961459278644
1101	32-33	0.4527044007055707
1101	34-35	0.5512546499203594
1101	36-37	0.46753322759605
1101	38-39	0.4277453054928344
1101	40-41	0.4232966002643792
1101	42-43	0.41764555776731527
1101	44-45	0.45072217133004244
1101	46-47	0.4376186289456001
1101	48-49	0.49597905358656647
1101	50-51	0.4990465983976833
1101	52-53	0.4218972073607645
1101	54-55	0.45507818290592184
1101	56-57	0.45113155544656536
1101	58-59	0.48479000609393097
1101	60-61	0.5285055194659876
1101	62-63	0.5554059715993986
1101	64-65	0.5733660764263604
1101	66-67	0.4724144647906101
1101	68-69	0.4615884289418233
1101	70-71	0.49928568521752936
1101	72-73	0.37775819267556443
1101	74-75	0.38068709510141474
1101	76-77	0.4462838928714916
1101	78-79	0.4275425045265493
1101	80-81	0.3943310204022694
1101	82-83	0.3568109954211991
1101	84-85	0.2622476385124912
1101	86-87	0.2578203772707255
1101	88-89	0.2930352987981877
1101	90-91	0.3758689183172095
1101	92-93	0.3890209045665003
1101	94-95	0.3721393389982772
1101	96-97	0.37230955009155764
1101	98-99	0.37955287776824775
1101	100	0.37048657847120126
1102	1	0.33592703348223907
1102	2	0.31685060984877467
1102	3	0.40304178192158346
1102	4	0.34976423146734703
1102	5	0.27693651834500344
1102	6	0.32634081183721975
1102	7	0.3375315626088664
1102	8	0.3107291132591854
1102	9	0.29349152483982976
1102	10-11	0.37202449816552274
1102	12-13	0.3658759987768647
1102	14-15	0.43968479390478166
1102	16-17	0.40686834626490054
1102	18-19	0.3590297681683552
1102	20-21	0.35752641781155603
1102	22-23	0.37784981422191777
1102	24-25	0.4310226967688422
1102	26-27	0.40627292380617064
1102	28-29	0.4409256620164612
1102	30-31	0.44813068945772727
1102	32-33	0.46968305025451684
1102	34-35	0.49287824148388637
1102	36-37	0.4354530478116132
1102	38-39	0.4793252682616682
1102	40-41	0.4712169767956169
1102	42-43	0.5422714748353457
1102	44-45	0.49995180532155814
1102	46-47	0.4245426650893265
1102	48-49	0.41381600375905236
1102	50-51	0.4672967704094404
1102	52-53	0.4392489557927135
1102	54-55	0.4235186568658875
1102	56-57	0.42670200206833897
1102	58-59	0.44767876447605204
1102	60-61	0.4683878167138573
1102	62-63	0.45164294213513756
1102	64-65	0.4568846208946127
1102	66-67	0.4504222038764638
1102	68-69	0.3531074489025343
1102	70-71	0.32844835664542416
1102	72-73	0.3708205425424964
1102	74-75	0.3050765255233756
1102	76-77	0.2513982183675978
1102	78-79	0.3739710890757735
1102	80-81	0.3476570009217781
1102	82-83	0.3419369773052452
1102	84-85	0.314282992898697
1102	86-87	0.31591768318084235
1102	88-89	0.3343091724819942
1102	90-91	0.32987541461482195
1102	92-93	0.32889632659229306
1102	94-95	0.39536559730824195
1102	96-97	0.32577912303530354
1102	98-99	0.2848468250011784
1102	100	0.2983158504376675
1103	1	0.21665857786975806
1103	2	0.2521700018859576
1103	3	0.30313584880211053
1103	4	0.22765988778260748
1103	5	0.2175248697147083
1103	6	0.173837133198262
1103	7	0.17595649513854994
1103	8	0.2287722614278067
1103	9	0.23655688117100482
1103	10-11	0.25941045587990885
1103	12-13	0.25258327285440174
1103	14-15	0.3060091939858651
1103	16-17	0.3538377318886532
1103	18-19	0.34292873836837146
1103	20-21	0.2997341713784252
1103	22-23	0.28950185274068474
1103	24-25	0.3096774159897038
1103	26-27	0.3643959218499049
1103	28-29	0.382685421194104
1103	30-31	0.343200815705174
1103	32-33	0.3957837287839112
1103	34-35	0.3928380195087371
1103	36-37	0.3965784142948081
1103	38-39	0.4031960253632292
1103	40-41	0.4212028364129665
1103	42-43	0.4569918569779361
1103	44-45	0.4162540743603955
1103	46-47	0.41330058820644666
1103	48-49	0.4633720743566698
1103	50-51	0.4195025755688988
1103	52-53	0.4221192902749564
1103	54-55	0.45281039485226415
1103	56-57	0.45147817155310577
1103	58-59	0.43791856685699315
1103	60-61	0.4503687549605857
1103	62-63	0.5214823268921549
1103	64-65	0.5130759491283214
1103	66-67	0.511332225566683
1103	68-69	0.5098506550755815
1103	70-71	0.5080656717181213
1103	72-73	0.46810923611742083
1103	74-75	0.4714715877136939
1103	76-77	0.44241627914895787
1103	78-79	0.3969593111619787
1103	80-81	0.41659885449408307
1103	82-83	0.4319192426184344
1103	84-85	0.43950553475836074
1103	86-87	0.4173243776021991
1103	88-89	0.3767656216458235
1103	90-91	0.40573478617083936
1103	92-93	0.34234464150349453
1103	94-95	0.3753664341880345
1103	96-97	0.3602282446747367
1103	98-99	0.3998311476853118
1103	100	0.364025840443869
1104	1	0.17235446265977572
1104	2	0.22682818230054025
1104	3	0.26423267236327774
1104	4	0.2079318288785501
1104	5	0.23432842873376813
1104	6	0.2113785602381668
1104	7	0.17647213733702216
1104	8	0.23596897631275482
1104	9	0.2666754906503783
1104	10-11	0.30811333012010067
1104	12-13	0.2745110880328596
1104	14-15	0.3626025147216225
1104	16-17	0.39222743249499814
1104	18-19	0.3455963355049292
1104	20-21	0.37574870411494743
1104	22-23	0.4850075051662941
1104	24-25	0.4670071316174287
1104	26-27	0.4132077501704856
1104	28-29	0.4525116824099982
1104	30-31	0.40163677199281267
1104	32-33	0.40044567773340134
1104	34-35	0.464401353029821
1104	36-37	0.4644355901183914
1104	38-39	0.4587470650399439
1104	40-41	0.4931680072761395
1104	42-43	0.4181386482414169
1104	44-45	0.3423471096067914
1104	46-47	0.32077026991453295
1104	48-49	0.33431388218720315
1104	50-51	0.3474510090884948
1104	52-53	0.35998862035359736
1104	54-55	0.3651929707518349
1104	56-57	0.3657006750470799
1104	58-59	0.3303167226955708
1104	60-61	0.3810433010456862
1104	62-63	0.30129642066641793
1104	64-65	0.39278525201247305
1104	66-67	0.39306288919616605
1104	68-69	0.36528382560494066
1104	70-71	0.3009593413895004
1104	72-73	0.3322321715470977
1104	74-75	0.34646268677520453
1104	76-77	0.3889103125060984
1104	78-79	0.41611483721309384
1104	80-81	0.37228086983233055
1104	82-83	0.3851174487166986
1104	84-85	0.37434713290349464
1104	86-87	0.3510358626762269
1104	88-89	0.3226654113952918
1104	90-91	0.3329100625096615
1104	92-93	0.3319874105788614
1104	94-95	0.2802141301824612
1104	96-97	0.3099273458589096
1104	98-99	0.35187913118475933
1104	100	0.33178419230277
1105	1	0.18103709739031615
1105	2	0.21207952834793886
1105	3	0.18503350766746252
1105	4	0.24911584739614057
1105	5	0.19840657496231984
1105	6	0.20386837053354157
1105	7	0.20433657652586845
1105	8	0.32971803282158874
1105	9	0.29977316085618355
1105	10-11	0.29756698583388186
1105	12-13	0.327667380489963
1105	14-15	0.3856941465839512
1105	16-17	0.38351209007060305
1105	18-19	0.3975229444718664
1105	20-21	0.3620913549477365
1105	22-23	0.4181944925360881
1105	24-25	0.33783186869789716
1105	26-27	0.33389788251085406
1105	28-29	0.37352134375956325
1105	30-31	0.2963613983703084
1105	32-33	0.3709697556280318
1105	34-35	0.4104103853852763
1105	36-37	0.34878621659652964
1105	38-39	0.363115429152046
1105	40-41	0.3867428324157771
1105	42-43	0.3770036912646191
1105	44-45	0.36342029936173503
1105	46-47	0.35235269330305385
1105	48-49	0.341610842887647
1105	50-51	0.33021845738517897
1105	52-53	0.3032552383780498
1105	54-55	0.29883031790626546
1105	56-57	0.3073256184376163
1105	58-59	0.28528703417345014
1105	60-61	0.23757414691696965
1105	62-63	0.23015404155908925
1105	64-65	0.24651204212971578
1105	66-67	0.25273744764229633
1105	68-69	0.31340061723176404
1105	70-71	0.27941566403832496
1105	72-73	0.2869754161503266
1105	74-75	0.3417515208717177
1105	76-77	0.30779837671381216
1105	78-79	0.27362691390005267
1105	80-81	0.30336890384833026
1105	82-83	0.24108031290429466
1105	84-85	0.2398361129266462
1105	86-87	0.2531882805295851
1105	88-89	0.2518519962088561
1105	90-91	0.25844219152893544
1105	92-93	0.23651868416614263
1105	94-95	0.15656835450691986
1105	96-97	0.19708580205575998
1105	98-99	0.21404765128740877
1105	100	0.19166065785905317
1106	1	0.0613906181089483
1106	2	0.12626382275535875
1106	3	0.14626938132726863
1106	4	0.09271190650944305
1106	5	0.06894410031728881
1106	6	0.049174079023835304
1106	7	0.11392799930187891
1106	8	0.13928772276653234
1106	9	0.1544964294739799
1106	10-11	0.1373561773725669
1106	12-13	0.17553031876872183
1106	14-15	0.23160113006699845
1106	16-17	0.14169812310451135
1106	18-19	0.1270325069175371
1106	20-21	0.06962178091121984
1106	22-23	0.12680069363722168
1106	24-25	0.16142647895579643
1106	26-27	0.19134499928859583
1106	28-29	0.15925736230491339
1106	30-31	0.14628533346158434
1106	32-33	0.13383559161759706
1106	34-35	0.11552846710072373
1106	36-37	0.11398470978690511
1106	38-39	0.08843055520247844
1106	40-41	0.09526572433115632
1106	42-43	0.17120102537067083
1106	44-45	0.08847463143939649
1106	46-47	0.07549453624712754
1106	48-49	0.1756480784715393
1106	50-51	0.10521483524667019
1106	52-53	0.0801713496501506
1106	54-55	0.07499664650667626
1106	56-57	0.05135995631069079
1106	58-59	0.11873386118011808
1106	60-61	0.1611868678674213
1106	62-63	0.15507073237334623
1106	64-65	0.20082600909581316
1106	66-67	0.18670948473299376
1106	68-69	0.1729558186226683
1106	70-71	0.17507271996414886
1106	72-73	0.15672693744849653
1106	74-75	0.21396247421857595
1106	76-77	0.21686458940578746
1106	78-79	0.19441436681225355
1106	80-81	0.21576748397003342
1106	82-83	0.1877914111366863
1106	84-85	0.20031495963774404
1106	86-87	0.1982042179390362
1106	88-89	0.173903575461253
1106	90-91	0.19959692928610195
1106	92-93	0.16834279312006828
1106	94-95	0.20229060892592798
1106	96-97	0.2012874827280271
1106	98-99	0.2123959561265849
1106	100	0.2648351000497229
1107	1	0.20850700427198632
1107	2	0.2551410772918423
1107	3	0.2370264670133615
1107	4	0.26371952670569954
1107	5	0.18767525540746988
1107	6	0.22027763694281077
1107	7	0.23093775753881118
1107	8	0.22707210958742508
1107	9	0.22899755331587102
1107	10-11	0.23943048799150546
1107	12-13	0.2603269307965732
1107	14-15	0.3286818822187456
1107	16-17	0.32257636442898985
1107	18-19	0.27561742550751944
1107	20-21	0.2825802261719019
1107	22-23	0.2817449127041556
1107	24-25	0.3173855820751399
1107	26-27	0.34167666970139976
1107	28-29	0.3083158020834347
1107	30-31	0.24097586577889274
1107	32-33	0.18155912651151596
1107	34-35	0.2694468378040824
1107	36-37	0.33556795631944425
1107	38-39	0.24794963310390017
1107	40-41	0.23941146743734976
1107	42-43	0.279592835030229
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2216	58-59	-0.5877571869848239
2216	60-61	-0.6176669467446416
2216	62-63	-0.5751490130423491
2216	64-65	-0.5912069351211429
2216	66-67	-0.5676221153362704
2216	68-69	-0.5902961911237696
2216	70-71	-0.5825708620085948
2216	72-73	-0.627871810040638
2216	74-75	-0.6199019468998443
2216	76-77	-0.6337115895211127
2216	78-79	-0.5471296364647174
2216	80-81	-0.5488982104922044
2216	82-83	-0.5304939031804778
2216	84-85	-0.5451912810982762
2216	86-87	-0.4992270297999539
2216	88-89	-0.47384284105984165
2216	90-91	-0.4303457941719415
2216	92-93	-0.4333067755111344
2216	94-95	-0.43399441119617066
2216	96-97	-0.4797499912483758
2216	98-99	-0.5450110381147901
2216	100	-0.5734299654825783
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	61744.0
3	8961.0
4	5741.0
5	4736.0
6	4007.0
7	4432.0
8	5011.0
9	5385.0
10	5789.0
11	6153.0
12	6581.0
13	6994.0
14	7651.0
15	7663.0
16	7756.0
17	7403.0
18	7494.0
19	7394.0
20	7475.0
21	7968.0
22	9301.0
23	12753.0
24	19285.0
25	24582.0
26	25303.0
27	23098.0
28	25297.0
29	31645.0
30	38529.0
31	46580.0
32	60232.0
33	80655.0
34	117192.0
35	184434.0
36	351266.0
37	695407.0
38	996114.0
39	370586.0
40	466.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.024085786655746	19.02928819523441	12.921508850161533	24.02511716794831
2	15.670825896026141	19.655179945419974	39.98684643209471	24.687147726459177
3	18.593634960182477	24.992833118908344	29.34171173332953	27.071820187579647
4	12.451521932230209	16.073088423024835	37.969794341353555	33.5055953033914
5	13.132134655579858	38.24848850719419	35.116535867807706	13.502840969418244
6	31.738571989353293	37.237116166699735	17.519713822700883	13.504598021246093
7	28.538678975695614	32.17869448332475	21.743109692207536	17.5395168487721
8	26.122111695613544	36.17055484991237	19.6429118555436	18.064421598930483
9	25.764609022538142	16.794096447821538	20.27231669404961	37.1689778355907
10-11	24.569436567742027	26.759767393288357	28.103813008205304	20.566983030764312
12-13	24.950822909539482	24.158877987274096	28.172493014949623	22.7178060882368
14-15	22.2390394340276	25.965306525157388	25.047439654075397	26.748214386739615
16-17	22.327908600334556	29.343230605728905	25.72795868225991	22.60090211167663
18-19	22.33119265136672	28.361290815755442	27.447534787977844	21.859981744899994
20-21	23.208547163069152	27.528979629475714	27.12668953044719	22.135783677007947
22-23	23.063284150751088	27.291327929720676	27.086705535467274	22.558682384060962
24-25	22.7061997258345	27.38905139890231	27.148982162861827	22.755766712401364
26-27	22.487970859479134	27.551767317503177	27.167308038820863	22.792953784196825
28-29	22.6655245177047	27.421023902773356	27.290211648918415	22.623239930603532
30-31	22.705932137835635	27.722881753113498	27.006523516358843	22.564662592692024
32-33	22.504187670745033	27.617134959785623	27.289856584789945	22.588820784679402
34-35	22.768126624745577	27.339997633976104	27.271443608247107	22.62043213303121
36-37	22.88994894858596	27.598005262416343	26.840871281915096	22.671174507082604
38-39	22.74576007442534	27.779222813903427	26.95730143219342	22.51771567947781
40-41	22.580566390697893	27.467604028236114	27.299265401111256	22.652564179954734
42-43	22.578286334862494	27.492964069926263	27.202623410327252	22.726126184883995
44-45	22.530280657767708	27.572595085171102	27.279719638004796	22.617404619056394
46-47	22.583341208220563	27.59824635994398	27.15335947239722	22.66505295943824
48-49	22.552070613683146	27.49114522737236	27.219472460329857	22.737311698614633
50-51	22.48593233687639	27.521788647089444	27.196673029526014	22.79560598650815
52-53	22.617084866723427	27.442566984895834	27.183982086090097	22.75636606229064
54-55	22.603686331866342	27.750577292937162	27.026714218342406	22.61902215685409
56-57	22.4872029868849	27.575837831590004	27.1504488245375	22.786510356987595
58-59	22.509297520347758	27.178823344534752	27.398402273573257	22.91347686154423
60-61	22.720646309275466	27.509037569563084	27.042859307570417	22.727456813591036
62-63	22.417157839907606	27.573695704867095	27.14490442333496	22.864242031890335
64-65	22.531296817434512	27.29903987144692	27.277137066810987	22.892526244307586
66-67	22.319430991211924	27.365937800612123	27.2638263753872	23.05080483278875
68-69	22.625074190480046	27.490164689593705	27.119018897356174	22.765742222570076
70-71	22.556783856183106	27.25251970327649	27.228827689745046	22.961868750795357
72-73	22.36342227758792	27.32370473769557	27.305063559743004	23.007809424973505
74-75	22.399869436628457	27.483999237369154	27.210101309471135	22.90603001653125
76-77	22.46352290537213	27.30375204024042	27.138333495374546	23.094391559012905
78-79	22.41217617079353	27.36917337219509	27.22037791013816	22.998272546873224
80-81	22.38221304868678	27.28517045211515	27.302962378493884	23.02965412070419
82-83	22.336147566918058	27.30187288959025	27.386023843174655	22.975955700317037
84-85	22.350493472368232	27.269184933716552	27.285232322352904	23.095089271562312
86-87	22.47137531943396	27.24031505349988	27.31707931184278	22.97123031522338
88-89	22.215573884743918	27.207711909690197	27.382727497892883	23.193986707673005
90-91	22.27305501620283	27.20536957702781	27.35485479640374	23.166720610365623
92-93	22.423193702340875	27.257717561157097	27.293693437906047	23.025395298595985
94-95	22.18920543013657	27.052052105189556	27.45305142601298	23.305691038660893
96-97	22.36112322349278	26.986029094488455	27.415598607383657	23.237249074635105
98-99	22.186448945123104	27.073429454046	27.428952937008294	23.311168663822603
100	22.229259543380987	26.939441728099144	27.427200884275805	23.404097844244063
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2809.0
1	1667.0
2	507.0
3	409.5
4	292.0
5	244.0
6	229.0
7	235.0
8	245.0
9	272.0
10	281.5
11	255.5
12	278.0
13	315.5
14	325.5
15	378.0
16	470.5
17	592.5
18	796.5
19	1077.5
20	1500.5
21	2164.5
22	3215.5
23	4993.5
24	7329.0
25	10180.5
26	14165.5
27	19239.0
28	25719.0
29	33581.5
30	41431.5
31	49467.0
32	57527.5
33	66013.0
34	74368.5
35	81656.0
36	89655.5
37	97638.0
38	104123.0
39	107615.5
40	110418.5
41	115040.0
42	118789.0
43	121481.0
44	121164.0
45	120318.5
46	121162.0
47	118924.0
48	115693.5
49	113381.5
50	111814.0
51	108666.0
52	101565.0
53	95935.5
54	93183.5
55	89285.5
56	82638.5
57	74259.0
58	67136.0
59	63148.5
60	58066.5
61	50969.0
62	44773.5
63	38993.0
64	32519.0
65	25761.5
66	20704.5
67	16368.5
68	12122.0
69	8866.0
70	6436.5
71	4746.5
72	3681.0
73	2737.0
74	1947.0
75	1344.5
76	917.5
77	651.0
78	441.5
79	302.5
80	215.5
81	135.5
82	94.5
83	73.5
84	64.0
85	49.5
86	49.0
87	39.0
88	22.0
89	22.5
90	21.5
91	13.5
92	7.0
93	10.0
94	9.5
95	7.5
96	5.0
97	2.5
98	2.5
99	1.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07617314370777399
2	0.07938617722668528
3	0.08032583797278196
4	0.08265983401953828
5	0.08041677288369455
6	0.08017427978792767
7	0.079446800500627
8	0.07947711213759787
9	0.07902243758303494
10-11	0.07873447703181176
12-13	0.07755232318994817
14-15	0.07597611806746339
16-17	0.07470302931468722
18-19	0.07426351057860975
20-21	0.07386945929798855
22-23	0.07587002733806537
24-25	0.07620345534474486
26-27	0.07532441787258988
28-29	0.07133843761092164
30-31	0.07662781826233692
32-33	0.07520317132470644
34-35	0.07253574727127066
36-37	0.06936818120781567
38-39	0.07082313978241701
40-41	0.07377852438707597
42-43	0.07852229557301574
44-45	0.07912852831243294
46-47	0.08243249674225682
48-49	0.08541819298388664
50-51	0.08641847700392505
52-53	0.08649425609635221
54-55	0.08667612591817737
56-57	0.08444822060081908
58-59	0.08347824821775152
60-61	0.08137158944827667
62-63	0.08196266636920847
64-65	0.08128065453736409
66-67	0.08075020089037402
68-69	0.08031068215429654
70-71	0.07861323048392832
72-73	0.07873447703181176
74-75	0.07818886756633626
76-77	0.07809793265542368
78-79	0.0785526072099866
80-81	0.07953773541153958
82-83	0.07899212594606407
84-85	0.07809793265542368
86-87	0.07902243758303494
88-89	0.07576393660866737
90-91	0.07323291492160047
92-93	0.07303588928128987
94-95	0.07347540801736736
96-97	0.07245996817884351
98-99	0.07199013780579516
100	0.07135359342940707
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3299063.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.81366801632915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.83564884489904	42.516877092606805
2	14.278181988103508	16.22391782697687
3	4.677911480391049	7.973079295701359
4	2.1151225951405497	4.806714917366071
5	1.1200187618725186	3.181618705454265
6	0.6746448000770575	2.299742742031242
7	0.44536533258758465	1.7711986706129141
8	0.29352003471095717	1.3340759846567785
9	0.2191393212532219	1.1205097782303828
>10	1.2321339865054575	12.627890887428164
>50	0.07676527342630529	2.9935646820827673
>100	0.030958747261030192	2.864901022680412
>500	5.35303429220232E-4	0.21915284441289296
>1k	5.3530342618188775E-5	0.06675554975910743
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.031163697086112E-5	0.0
2	0.0	0.0	0.0	3.031163697086112E-5	0.0
3	0.0	0.0	0.0	3.031163697086112E-5	0.0
4	0.0	0.0	0.0	9.093491091258337E-5	0.0
5	0.0	0.0	0.0	1.2124654788344448E-4	0.0
6	0.0	0.0	0.0	1.2124654788344448E-4	0.0
7	0.0	0.0	0.0	1.2124654788344448E-4	0.0
8	0.0	0.0	0.0	1.2124654788344448E-4	0.0
9	0.0	0.0	0.0	1.515581848543056E-4	0.0
10-11	0.0	0.0	0.0	1.515581848543056E-4	0.0
12-13	0.0	0.0	0.0	1.515581848543056E-4	0.0
14-15	0.0	0.0	0.0	1.515581848543056E-4	0.0
16-17	0.0	0.0	0.0	1.818698218251667E-4	0.0
18-19	0.0	0.0	0.0	2.273372772814584E-4	0.0
20-21	0.0	0.0	0.0	2.576489142523195E-4	0.0
22-23	0.0	0.0	0.0	3.637396436503334E-4	0.0
24-25	0.0	0.0	0.0	5.001420100192085E-4	0.0
26-27	0.0	0.0	0.0	6.668560133589447E-4	0.0
28-29	0.0	0.0	0.0	0.001758074944309945	0.0
30-31	0.0	0.0	0.0	0.005804678479919905	0.0
32-33	0.0	0.0	0.0	0.014549585746013338	0.0
34-35	0.0	0.0	0.0	0.026355968346163744	0.0
36-37	0.0	0.0	0.0	0.04980201954312482	0.0
38-39	0.0	0.0	0.0	0.092647518401437	0.0
40-41	0.0	0.0	0.0	0.1449502479946579	0.0
42-43	0.0	0.0	0.0	0.19573739573933568	0.0
44-45	0.0	0.0	0.0	0.2553300740240486	0.0
46-47	0.0	0.0	0.0	0.3286084564011054	0.0
48-49	0.0	0.0	0.0	0.412404976807051	0.0
50-51	3.031163697086112E-5	0.0	0.0	0.5070227516115939	0.0
52-53	3.031163697086112E-5	0.0	0.0	0.6005644633036714	0.0
54-55	3.031163697086112E-5	0.0	0.0	0.7011990980469303	0.0
56-57	3.031163697086112E-5	0.0	0.0	0.8210058431742588	0.0
58-59	3.031163697086112E-5	0.0	0.0	0.9588025448437936	0.0
60-61	3.031163697086112E-5	0.0	0.0	1.109542315499886	0.0
62-63	3.031163697086112E-5	0.0	0.0	1.265904894814073	0.0
64-65	3.031163697086112E-5	0.0	0.0	1.4235860303364927	0.0
66-67	3.031163697086112E-5	0.0	0.0	1.6016365859033308	0.0
68-69	3.031163697086112E-5	0.0	0.0	1.8134543050557084	0.0
70-71	3.031163697086112E-5	0.0	0.0	2.0430801109284666	0.0
72-73	3.031163697086112E-5	0.0	0.0	2.282102524262192	0.0
74-75	3.031163697086112E-5	0.0	0.0	2.519776069750714	0.0
76-77	3.031163697086112E-5	0.0	0.0	2.7716506171600845	0.0
78-79	3.031163697086112E-5	0.0	0.0	3.061323775872119	0.0
80-81	3.031163697086112E-5	0.0	0.0	3.384021463063906	0.0
82-83	3.031163697086112E-5	0.0	0.0	3.7181163257567373	0.0
84-85	3.031163697086112E-5	1.515581848543056E-5	0.0	4.042435685526466	0.0
86-87	3.031163697086112E-5	3.031163697086112E-5	0.0	4.376985222773861	0.0
88	3.031163697086112E-5	3.031163697086112E-5	0.0	4.651896614281085	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2925	0.0	40.495255	1
GTATCAA	4755	0.0	32.521824	1
TCAACGC	4970	0.0	30.07508	4
CAACGCA	5160	0.0	28.874819	5
TAGACCG	115	4.1986277E-6	28.609476	5
ATCAACG	5165	0.0	28.575167	3
GTACATG	11750	0.0	28.282059	1
AACGCAG	5270	0.0	28.182505	6
TACATGG	11665	0.0	27.400194	2
TATCAAC	5910	0.0	25.29118	2
ACATGGG	12100	0.0	25.133234	3
ACGCAGA	6510	0.0	22.525278	7
GAGTACA	8245	0.0	21.492165	1
CGCAGAG	7015	0.0	20.702715	8
CATGGGG	8370	0.0	20.048357	4
AGTACAT	8690	0.0	19.093449	2
CATGGGA	4100	0.0	17.425922	4
TATACTG	1765	0.0	17.042957	5
GCAGAGT	8150	0.0	16.896881	9
GTATAGA	1380	0.0	16.689621	1
>>END_MODULE