FastQCFastQC Report
Wed 25 May 2016
SRR1032953_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032953_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3299063
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58730.17802024392986737No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52010.15765082388544868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47000.051.1800961
GTATCAA89950.044.187371
TCAACGC105750.036.9294054
ATCAACG106450.036.6865653
CAACGCA108850.035.8733185
TATCAAC112600.034.9753232
AACGCAG112500.034.7512476
ACGCAGA131200.029.6538017
CGCAGAG133850.028.890098
GTACATG115550.028.7867261
TACATGG116400.027.082982
ACATGGG117400.026.1709633
GCAGAGT149050.025.779319
GTGGTAT23850.023.4416161
CATGGGG82150.021.7073214
CAGAGTA143450.021.02250510-11
GAGTACT91000.020.85402712-13
GAGTACA83150.020.8493751
AGAGTAC137100.020.21457110-11
TGGTATC25700.019.4065062
Produced by FastQC (version 0.11.5)