FastQCFastQC Report
Wed 25 May 2016
SRR1032952_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032952_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2639531
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26050.037.7091
GTATCAA42500.032.9559481
TCAACGC41900.032.081784
ATCAACG42150.032.0030063
CAACGCA43150.031.1518235
AACGCAG45700.029.5164366
GTACATG90650.028.205741
TATCAAC50000.027.824552
TACATGG91100.026.7250082
ACATGGG92100.025.363153
ACGCAGA55300.024.0520047
CGCAGAG58100.022.4884058
CATGGGG64050.021.0605244
TACCTGG17400.019.7187792
GCAGAGT66000.019.2977689
GTATAGA11400.018.5529981
GAGTACA63800.018.1962851
GTACCGC3106.373739E-918.1935636
TACCGCT3101.2616329E-716.6771167
CATGGGA31650.016.483744
Produced by FastQC (version 0.11.5)