##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1032952_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2639531
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32779535455352	33.0	31.0	34.0	28.0	34.0
2	31.363279688702274	33.0	31.0	34.0	28.0	34.0
3	31.430824263855964	34.0	31.0	34.0	28.0	34.0
4	34.78353048325631	37.0	35.0	37.0	32.0	37.0
5	34.84068457616144	37.0	35.0	37.0	32.0	37.0
6	34.913406965101	37.0	35.0	37.0	32.0	37.0
7	34.820696555562336	37.0	35.0	37.0	32.0	37.0
8	34.81592260140154	37.0	35.0	37.0	32.0	37.0
9	36.411825812994806	39.0	37.0	39.0	32.0	39.0
10-11	36.4099156251622	39.0	37.0	39.0	32.0	39.0
12-13	36.38164185228361	39.0	37.0	39.0	32.0	39.0
14-15	37.81904493639211	41.0	38.5	41.0	33.0	41.0
16-17	37.85296194664886	41.0	39.0	41.0	33.0	41.0
18-19	37.856755234168496	41.0	39.0	41.0	33.0	41.0
20-21	37.878049547438536	41.0	39.0	41.0	33.0	41.0
22-23	37.87593004211733	41.0	39.0	41.0	33.0	41.0
24-25	37.90867563214829	41.0	39.0	41.0	33.0	41.0
26-27	37.8808648203033	41.0	39.0	41.0	32.5	41.0
28-29	37.86352954369545	41.0	38.5	41.0	32.0	41.0
30-31	37.823305162924775	41.0	38.0	41.0	32.0	41.0
32-33	37.76467656564746	40.5	38.0	41.0	32.0	41.0
34-35	37.69655480462249	40.0	38.0	41.0	32.0	41.0
36-37	37.614290947899455	40.0	38.0	41.0	32.0	41.0
38-39	37.521991785661925	40.0	38.0	41.0	31.0	41.0
40-41	37.43197749903297	40.0	38.0	41.0	31.0	41.0
42-43	37.324289807545355	40.0	38.0	41.0	31.0	41.0
44-45	37.197024963904575	40.0	38.0	41.0	31.0	41.0
46-47	37.07002645545743	40.0	37.5	41.0	30.0	41.0
48-49	36.93936138654935	40.0	37.0	41.0	30.0	41.0
50-51	36.802814780353025	40.0	37.0	41.0	30.0	41.0
52-53	36.64523299783181	40.0	36.5	41.0	30.0	41.0
54-55	36.47763731511394	40.0	36.0	41.0	30.0	41.0
56-57	36.29588930760806	40.0	35.5	41.0	30.0	41.0
58-59	36.08493990030804	39.0	35.0	41.0	29.5	41.0
60-61	35.84341706916872	39.0	35.0	41.0	29.0	41.0
62-63	35.590055960699075	39.0	35.0	41.0	29.0	41.0
64-65	35.31163339244737	38.0	35.0	40.0	28.0	41.0
66-67	35.01185172668932	37.5	35.0	40.0	28.0	41.0
68-69	34.70086939687391	37.0	34.0	40.0	28.0	41.0
70-71	34.365949291749175	36.5	34.0	39.5	28.0	41.0
72-73	34.07321509010502	36.0	34.0	39.0	27.5	41.0
74-75	33.79803950019909	36.0	34.0	39.0	27.0	40.5
76-77	33.47342008864453	35.0	34.0	38.0	27.0	40.0
78-79	33.10700253188919	35.0	34.0	37.0	26.0	39.0
80-81	32.78913242541951	35.0	33.5	37.0	26.0	39.0
82-83	32.47307305729692	35.0	33.0	36.0	26.0	38.5
84-85	32.30454671682204	35.0	33.0	36.0	26.0	37.0
86-87	32.07539237084164	35.0	33.0	36.0	26.0	37.0
88-89	31.843161531347803	35.0	33.0	35.0	25.0	37.0
90-91	31.6463777087672	35.0	33.0	35.0	25.0	36.0
92-93	31.455901635555712	35.0	33.0	35.0	25.0	36.0
94-95	31.275296255281717	35.0	33.0	35.0	24.0	36.0
96-97	31.09854042252203	35.0	33.0	35.0	23.5	36.0
98-99	30.915638990411555	35.0	33.0	35.0	21.5	35.0
100	30.781018673393113	35.0	33.0	35.0	19.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.49978497239012043
1101	2	0.5767815202155617
1101	3	0.5520870260145436
1101	4	0.5322255761791226
1101	5	0.4523332359559973
1101	6	0.4742085931156197
1101	7	0.5421767502894852
1101	8	0.49867773973053886
1101	9	0.5784004334436901
1101	10-11	0.5788084401663056
1101	12-13	0.43404328209140175
1101	14-15	0.5328052997551396
1101	16-17	0.4920805297263797
1101	18-19	0.5255187650269946
1101	20-21	0.44513445540474095
1101	22-23	0.48948562805536966
1101	24-25	0.49105386296933773
1101	26-27	0.4747446158329822
1101	28-29	0.46338566475260023
1101	30-31	0.5032575920162969
1101	32-33	0.4667235613539731
1101	34-35	0.512784055136386
1101	36-37	0.4873504469870795
1101	38-39	0.433290054069964
1101	40-41	0.5191621811018621
1101	42-43	0.5000839278561102
1101	44-45	0.5405562853498438
1101	46-47	0.51366587631383
1101	48-49	0.5727482343581158
1101	50-51	0.6202680617354943
1101	52-53	0.5957281247007487
1101	54-55	0.6238760723460857
1101	56-57	0.6016188900548016
1101	58-59	0.5898111407464128
1101	60-61	0.604995764595472
1101	62-63	0.6614725708883782
1101	64-65	0.5967268725649291
1101	66-67	0.6929820291935513
1101	68-69	0.5850277288749126
1101	70-71	0.5866787100511246
1101	72-73	0.5878005316343149
1101	74-75	0.5566347009045032
1101	76-77	0.5400982035163011
1101	78-79	0.5963098262283424
1101	80-81	0.6317460428949886
1101	82-83	0.5837399866855364
1101	84-85	0.4992506688366163
1101	86-87	0.47725798555729426
1101	88-89	0.5310717649298873
1101	90-91	0.5452962824529699
1101	92-93	0.5627637315175704
1101	94-95	0.5244005327147043
1101	96-97	0.5590291314447633
1101	98-99	0.5689455727932611
1101	100	0.5881460584611702
1102	1	0.45297901588928013
1102	2	0.4498884544076276
1102	3	0.49787975066886503
1102	4	0.47948118953825514
1102	5	0.39756953169033693
1102	6	0.42209280752202005
1102	7	0.44985057622564995
1102	8	0.4680589716415611
1102	9	0.5633136130622489
1102	10-11	0.4935955288335947
1102	12-13	0.527483657835468
1102	14-15	0.6606788946614515
1102	16-17	0.6091377124221822
1102	18-19	0.6053482127252536
1102	20-21	0.5957246819074342
1102	22-23	0.5523202393412703
1102	24-25	0.6448998587166415
1102	26-27	0.6160061403502084
1102	28-29	0.6285586529624467
1102	30-31	0.6344328443598499
1102	32-33	0.657581329062964
1102	34-35	0.6666983100643122
1102	36-37	0.7121735007723871
1102	38-39	0.7251815274271891
1102	40-41	0.6280283411723673
1102	42-43	0.7152047711111322
1102	44-45	0.7073578893892005
1102	46-47	0.67814900017013
1102	48-49	0.6821528396038588
1102	50-51	0.6439162908346461
1102	52-53	0.6167644421420633
1102	54-55	0.6319781248407565
1102	56-57	0.6440721479307143
1102	58-59	0.5645298690498137
1102	60-61	0.6890665698000475
1102	62-63	0.6112920338534451
1102	64-65	0.5218577369158766
1102	66-67	0.5430743944970899
1102	68-69	0.6005286892068895
1102	70-71	0.5304585200842808
1102	72-73	0.5646477843465618
1102	74-75	0.5020404786113417
1102	76-77	0.4387173127826074
1102	78-79	0.42277008851593223
1102	80-81	0.4735522495872644
1102	82-83	0.46169976523014356
1102	84-85	0.4504973296899877
1102	86-87	0.4123721997273293
1102	88-89	0.38638720257953807
1102	90-91	0.42983451482713875
1102	92-93	0.4689848266003587
1102	94-95	0.5301182568532319
1102	96-97	0.5082732726766821
1102	98-99	0.5456796662500771
1102	100	0.5770111561073712
1103	1	0.11741912302015223
1103	2	0.22202211289286566
1103	3	0.19836247354865222
1103	4	0.12480120577865961
1103	5	0.05854255165520783
1103	6	-8.599010147065655E-4
1103	7	0.08350253982396083
1103	8	0.09984119549436343
1103	9	0.15752442682521917
1103	10-11	0.1581524946985624
1103	12-13	0.06348490665417472
1103	14-15	0.161130139241088
1103	16-17	0.19470488526435759
1103	18-19	0.16901620288090413
1103	20-21	0.1383706882128024
1103	22-23	0.16079771487714112
1103	24-25	0.17719753499474677
1103	26-27	0.2029645084593099
1103	28-29	0.24605969301277497
1103	30-31	0.2531022821684843
1103	32-33	0.2468115795956507
1103	34-35	0.25336619966533647
1103	36-37	0.23406915722196686
1103	38-39	0.16650084964349787
1103	40-41	0.22580854088932512
1103	42-43	0.26999627495791856
1103	44-45	0.24061690127542334
1103	46-47	0.28548162411053823
1103	48-49	0.33307676676712106
1103	50-51	0.34205736488242877
1103	52-53	0.2943745892718326
1103	54-55	0.277516805688407
1103	56-57	0.24715304010960892
1103	58-59	0.2594630912220097
1103	60-61	0.25470936639563035
1103	62-63	0.3274560841028489
1103	64-65	0.2910042177086538
1103	66-67	0.25194046134453174
1103	68-69	0.3016537180424095
1103	70-71	0.31604329377798024
1103	72-73	0.34319039744131885
1103	74-75	0.2349324661061729
1103	76-77	0.22003025895585182
1103	78-79	0.2300029995754329
1103	80-81	0.21207434832463434
1103	82-83	0.23520478863905225
1103	84-85	0.16903631948149211
1103	86-87	0.18034216862515962
1103	88-89	0.12488116725437592
1103	90-91	0.15552729147104216
1103	92-93	0.19512285701274834
1103	94-95	0.1351349772830055
1103	96-97	0.17433651046593468
1103	98-99	0.2076107989183349
1103	100	0.19024370529155377
1104	1	-0.012609583437637184
1104	2	0.11826509760139814
1104	3	0.05285290321190672
1104	4	-0.04688402067833408
1104	5	0.0077101684353451105
1104	6	-0.047303648829711165
1104	7	-0.03315012504378956
1104	8	-0.05846423179367122
1104	9	-0.02917117127223179
1104	10-11	0.06172073500555797
1104	12-13	0.069731411925126
1104	14-15	0.09437693023256344
1104	16-17	0.06665862686635649
1104	18-19	0.050281550242338824
1104	20-21	0.019391287115965383
1104	22-23	0.10472816989538813
1104	24-25	0.10103921432035179
1104	26-27	0.0594971100089694
1104	28-29	0.06834846613289614
1104	30-31	0.056543436820462034
1104	32-33	0.07556418334493742
1104	34-35	0.11922825547434712
1104	36-37	0.10735080553888565
1104	38-39	0.10299559792111523
1104	40-41	0.10029593847543339
1104	42-43	0.05108630235789491
1104	44-45	0.03550620372281088
1104	46-47	0.02892602184217452
1104	48-49	0.00878816678466876
1104	50-51	9.38857924630554E-4
1104	52-53	0.02261466648140953
1104	54-55	0.054081515750006304
1104	56-57	0.04161784452979589
1104	58-59	0.006972755787266749
1104	60-61	0.056235167450040535
1104	62-63	0.01099014922949948
1104	64-65	0.04467305996534776
1104	66-67	0.02642032932055116
1104	68-69	0.12467218408595926
1104	70-71	0.16465325694069577
1104	72-73	0.16850300994286727
1104	74-75	0.15121855747112534
1104	76-77	0.13765495168345154
1104	78-79	0.12482923250572497
1104	80-81	0.13111319142966948
1104	82-83	0.056988745482392744
1104	84-85	0.013705052045786204
1104	86-87	0.026703220799291216
1104	88-89	0.06273471388137963
1104	90-91	0.07538510612961957
1104	92-93	0.03823035166289657
1104	94-95	0.03063959773212588
1104	96-97	-0.040787773624956
1104	98-99	-0.036215787946989764
1104	100	-0.07396315867787706
1105	1	0.007248285583077774
1105	2	0.08680808621608449
1105	3	0.08014449814138302
1105	4	0.10878148269252819
1105	5	0.024471897258244724
1105	6	0.07841277733514573
1105	7	0.09414488835884072
1105	8	-0.01737902849554729
1105	9	0.11352281808316889
1105	10-11	0.1427441192995147
1105	12-13	0.19559384979748273
1105	14-15	0.2612430539663393
1105	16-17	0.20879680293516145
1105	18-19	0.19004596346626812
1105	20-21	0.1956965487412674
1105	22-23	0.28485302166928506
1105	24-25	0.2951078727620171
1105	26-27	0.27272755606573895
1105	28-29	0.2524007560393571
1105	30-31	0.23251719650982494
1105	32-33	0.23605783024405724
1105	34-35	0.2602693369408513
1105	36-37	0.24771504316124293
1105	38-39	0.20633521091119889
1105	40-41	0.24879859879489885
1105	42-43	0.33198947265265843
1105	44-45	0.30434316718860543
1105	46-47	0.24868114982869827
1105	48-49	0.15590105168813295
1105	50-51	0.24694827491855875
1105	52-53	0.28984543196473567
1105	54-55	0.22153403910910896
1105	56-57	0.20315128854600317
1105	58-59	0.20466952836110153
1105	60-61	0.24584607462146835
1105	62-63	0.18904592514816443
1105	64-65	0.21925216564848427
1105	66-67	0.268469057648538
1105	68-69	0.35762334503819204
1105	70-71	0.33444220981947126
1105	72-73	0.19994358636110832
1105	74-75	0.2568811406078382
1105	76-77	0.18211520522066849
1105	78-79	0.24395073888152297
1105	80-81	0.21851867133901237
1105	82-83	0.16613471124119172
1105	84-85	0.11300470792871664
1105	86-87	0.11041280531225084
1105	88-89	0.21706562872568824
1105	90-91	0.2804783750867834
1105	92-93	0.23526121397067357
1105	94-95	0.16745142587067008
1105	96-97	0.20964621330568178
1105	98-99	0.19725973495484084
1105	100	0.20149090313254803
1106	1	-0.05781405307322984
1106	2	-0.004693739438646816
1106	3	-0.04948280189815435
1106	4	-0.10201054700845447
1106	5	-0.03770369753308955
1106	6	-0.04103004912088437
1106	7	-0.1400451478159752
1106	8	-0.12283648873179231
1106	9	-0.06073893873158909
1106	10-11	0.02921095147670627
1106	12-13	0.0020616307442864468
1106	14-15	0.01436566882824053
1106	16-17	-0.02907427433855503
1106	18-19	0.016241809599627288
1106	20-21	0.016661212226694033
1106	22-23	-0.03455799854083352
1106	24-25	0.008445987823719747
1106	26-27	0.025020592149637366
1106	28-29	0.007641915303679525
1106	30-31	-0.03539505842219626
1106	32-33	-0.033889191940765784
1106	34-35	-0.01879287697472165
1106	36-37	0.08480720436856615
1106	38-39	0.0843866019818833
1106	40-41	0.11761937330803818
1106	42-43	0.10176900660857058
1106	44-45	0.034090371621765314
1106	46-47	-0.009095391406575004
1106	48-49	0.04535366712089228
1106	50-51	-0.01894513570469769
1106	52-53	0.002595521291787861
1106	54-55	-0.0490200370588596
1106	56-57	-0.0054542104063841634
1106	58-59	0.08986899990435404
1106	60-61	0.01106873948538123
1106	62-63	-0.028378968769345647
1106	64-65	-0.019940383840818754
1106	66-67	0.01781826512198137
1106	68-69	0.02868452402847055
1106	70-71	0.11921706071200333
1106	72-73	0.007234405125501553
1106	74-75	0.020395557677446163
1106	76-77	0.10271792234883748
1106	78-79	0.0321937523429483
1106	80-81	-0.010646387616844777
1106	82-83	-0.009442241109958616
1106	84-85	0.02273720775428245
1106	86-87	0.05855362650802931
1106	88-89	0.05555596977533028
1106	90-91	-0.05097488360648228
1106	92-93	-0.04531724742629706
1106	94-95	-0.0211477858312783
1106	96-97	0.0017172082293654967
1106	98-99	-0.0517114842907489
1106	100	0.025858319582681588
1107	1	0.17662093040232918
1107	2	0.2060015423869821
1107	3	0.18310169557374678
1107	4	0.17959582298203713
1107	5	0.1188479205436721
1107	6	0.11055388027440927
1107	7	0.12038433065203691
1107	8	0.16793750779697802
1107	9	0.17772355095044645
1107	10-11	0.07086923299790016
1107	12-13	0.13745700892324209
1107	14-15	0.2141098774284913
1107	16-17	0.2145755393888109
1107	18-19	0.22572912830943181
1107	20-21	0.2568933703842333
1107	22-23	0.28178638142395585
1107	24-25	0.21475716198470707
1107	26-27	0.2550539548806796
1107	28-29	0.27069063493433276
1107	30-31	0.2689532070610312
1107	32-33	0.21675957665119228
1107	34-35	0.24404763159508036
1107	36-37	0.20916005212716726
1107	38-39	0.2475752379389391
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2216	50-51	-0.47372217263042415
2216	52-53	-0.5215122836606483
2216	54-55	-0.5029184270695666
2216	56-57	-0.5156767704418641
2216	58-59	-0.43253789199215475
2216	60-61	-0.5371813129319918
2216	62-63	-0.49442395459823274
2216	64-65	-0.5800060601857027
2216	66-67	-0.6525531580203037
2216	68-69	-0.608609527051847
2216	70-71	-0.5933160169391698
2216	72-73	-0.6211572891937962
2216	74-75	-0.5904536216306084
2216	76-77	-0.5290340102836169
2216	78-79	-0.4849492406879392
2216	80-81	-0.3836106230031149
2216	82-83	-0.4194559578266066
2216	84-85	-0.5156181304555538
2216	86-87	-0.4589683458053244
2216	88-89	-0.39248412287223644
2216	90-91	-0.46451932069971846
2216	92-93	-0.5121002073477143
2216	94-95	-0.5654284642851373
2216	96-97	-0.6138216233868263
2216	98-99	-0.5659434430570869
2216	100	-0.5743645910184014
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	49556.0
3	7307.0
4	4584.0
5	3911.0
6	3288.0
7	3454.0
8	3914.0
9	4044.0
10	4446.0
11	4895.0
12	5283.0
13	5544.0
14	6038.0
15	6126.0
16	5994.0
17	5832.0
18	5886.0
19	5866.0
20	5814.0
21	6140.0
22	7219.0
23	10609.0
24	16325.0
25	20958.0
26	20756.0
27	18071.0
28	20018.0
29	24336.0
30	29775.0
31	36156.0
32	46259.0
33	62007.0
34	89146.0
35	139154.0
36	259658.0
37	532730.0
38	821981.0
39	335967.0
40	484.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.53519249762565	19.590152280607303	13.204489747172108	23.67016547459494
2	15.441881521132078	19.934043176771187	40.52488679547887	24.099188506617864
3	18.279777815693187	25.58539498379116	29.72655405789683	26.408273142618814
4	12.384844368090047	16.63583463922357	38.67026312566614	32.30905786702024
5	12.914595537356158	38.539460463705474	35.29048891922122	13.255455079717152
6	31.042799311000895	38.059048724733636	17.740968640531058	13.157183323734412
7	27.72461660256488	32.85138192936266	22.06802574586574	17.35597572220672
8	25.400495320540333	36.976224310744634	19.896082781923987	17.72719758679105
9	25.23819074748046	17.444939065102503	20.704632856958156	36.61223733045888
10-11	24.093312624324668	27.411739793694746	28.335626224763267	20.15932135721732
12-13	24.474331897201335	24.73177026412456	28.546161372150287	22.24773646652382
14-15	21.795212534037216	26.642222301420183	25.475800685028627	26.086764479513974
16-17	21.746939806140333	30.025955630506473	26.123208489000138	22.10389607435306
18-19	21.767555512165902	29.021069363312634	27.861737812535843	21.34963731198562
20-21	22.756620054401512	28.221869756736258	27.534315623933104	21.487194564929126
22-23	22.56495552982886	27.877338648357785	27.53184815707126	22.025857664742094
24-25	22.27655916179947	27.973615716070377	27.547160154006267	22.202664968123887
26-27	22.115156222186794	27.97789331747532	27.612349001838243	22.294601458499642
28-29	22.14208224549144	28.026484673158365	27.690336551885725	22.141096529464473
30-31	22.24706857859758	28.32456191235555	27.393638700866678	22.03473080818019
32-33	22.08549519918865	28.092167995222884	27.697847454574752	22.124489351013715
34-35	22.210174447149296	27.841860567392075	27.771873946741167	22.176091038717463
36-37	22.43041196661685	28.095436317026177	27.33537220148476	22.138779514872212
38-39	22.208997266547623	28.31356224575291	27.453889577622842	22.02355091007662
40-41	22.07237349689474	28.025953671165286	27.736261019865232	22.165411812074737
42-43	22.045284920325134	28.00153023667673	27.658288595585102	22.294896247413035
44-45	22.0811734302345	28.147114045916837	27.6919798715736	22.079732652275062
46-47	22.13515781834534	28.178268348156422	27.578393510083004	22.108180323415233
48-49	22.07537003641592	28.0295096363413	27.619982193964454	22.275138133278325
50-51	22.115553240044264	28.007992305727875	27.634597151276513	22.24185730295135
52-53	22.10569622053066	28.005270591804038	27.63740982643069	22.251623361234607
54-55	22.162847040993206	28.30768536580418	27.45402125714065	22.075446336061965
56-57	22.028250573402538	28.064836101097818	27.53623270834447	22.370680617155173
58-59	22.010281905919634	27.698507385155146	27.846629468995694	22.44458123992953
60-61	22.272620849751696	28.035303473947526	27.47938087162916	22.212694804671614
62-63	22.02189013150648	28.03268068894405	27.566596401719345	22.378832777830123
64-65	22.022401787198756	27.874988601565125	27.638943840456413	22.463665770779702
66-67	21.896438892591977	27.89752782814231	27.557519835537363	22.648513443728348
68-69	22.136583753057884	27.97291620220183	27.550196326443327	22.34030371829696
70-71	22.171102743769392	27.785048964873216	27.617371430477995	22.4264768608794
72-73	21.899030272491668	27.874476278330164	27.711992465573847	22.514500983604318
74-75	21.995985984500145	27.966146838958057	27.588821040766316	22.449046135775486
76-77	22.091711897151463	27.839463706197076	27.536962426106903	22.53186197054456
78-79	22.038875093199106	27.925096450291818	27.62086878911969	22.415159667389382
80-81	21.982684914969617	27.79871535930849	27.711624288285474	22.50697543743642
82-83	21.908218110235026	27.76173349530539	27.84016027347192	22.489888120987665
84-85	21.88684710698837	27.771410242459655	27.68596984050493	22.655772810047047
86-87	22.086980028932807	27.767720869515927	27.682810611071396	22.46248849047987
88-89	21.825502292081428	27.71846999441529	27.724801606333738	22.731226107169547
90-91	21.854198304121823	27.68115170036137	27.74141412150034	22.72323587401647
92-93	21.98893816926834	27.74008925062798	27.698707380512456	22.572265199591225
94-95	21.799550391061963	27.490464434772864	27.82517836592041	22.884806808244765
96-97	21.914564865503745	27.4683585335898	27.825420352334106	22.79165624857235
98-99	21.830936255523845	27.60119211906329	27.783588913793157	22.78428271161971
100	21.90311904066847	27.38204551744532	27.731217324380985	22.983618117505223
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2438.0
1	1548.0
2	556.0
3	418.5
4	334.5
5	283.0
6	253.0
7	235.0
8	250.5
9	273.0
10	278.5
11	270.0
12	277.0
13	284.0
14	326.0
15	401.5
16	472.0
17	620.0
18	835.5
19	1155.0
20	1582.0
21	2262.0
22	3410.5
23	5186.5
24	7590.0
25	10565.0
26	14652.0
27	19806.5
28	26190.0
29	33721.0
30	41182.0
31	47945.5
32	54113.5
33	60856.5
34	67310.0
35	72447.5
36	77541.0
37	82953.5
38	87543.0
39	90310.0
40	92717.0
41	94986.5
42	95750.0
43	95959.0
44	94858.5
45	93302.0
46	92479.0
47	90375.0
48	88258.5
49	86533.5
50	84536.5
51	82190.0
52	77537.0
53	73301.5
54	70671.0
55	66753.5
56	61186.5
57	55223.0
58	49897.0
59	45793.0
60	41247.5
61	35606.0
62	30961.0
63	26529.0
64	21774.5
65	17546.0
66	13936.0
67	10705.0
68	8170.5
69	6293.0
70	4601.5
71	3286.5
72	2504.5
73	1891.0
74	1404.5
75	972.0
76	637.0
77	470.0
78	352.0
79	228.5
80	152.0
81	109.0
82	82.0
83	64.0
84	48.0
85	39.0
86	35.0
87	29.5
88	25.0
89	19.5
90	16.0
91	10.5
92	6.0
93	4.0
94	1.5
95	1.0
96	1.5
97	1.0
98	1.0
99	1.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07448292897488229
2	0.07758954147536058
3	0.07883976357921162
4	0.08039306982945076
5	0.07883976357921162
6	0.07887764909751012
7	0.07800628217664426
8	0.07755165595706207
9	0.07743799940216652
10-11	0.07696443042343507
12-13	0.07601729246597218
14-15	0.07489966967616596
16-17	0.07399041723700157
18-19	0.07325164963018051
20-21	0.0727591378922998
22-23	0.0748807269170167
24-25	0.07486178415786744
26-27	0.07416090206934489
28-29	0.07025869368459776
30-31	0.07531641037744963
32-33	0.07410407379189712
34-35	0.07137631647440398
36-37	0.06792873430923903
38-39	0.0697472391875678
40-41	0.07228556891356835
42-43	0.07692654490513655
44-45	0.07830936632303238
46-47	0.08135915054606292
48-49	0.08421950717760086
50-51	0.08503404582101896
52-53	0.08546972928145188
54-55	0.08533712996740708
56-57	0.08349968232992906
58-59	0.08257148713161543
60-61	0.08007104292391337
62-63	0.08079086777158519
64-65	0.07984372981412229
66-67	0.0792943897987938
68-69	0.0790670766890027
70-71	0.07683183110939026
72-73	0.07719174353322616
74-75	0.07662346075874843
76-77	0.07658557524044991
78-79	0.07700231594173358
80-81	0.0781199387315398
82-83	0.07724857181067393
84-85	0.07652874696300214
86-87	0.07730540008812171
88-89	0.07471024208467338
90-91	0.07200142752632949
92-93	0.07207719856292652
94-95	0.07219085511782207
96-97	0.07169834337994137
98-99	0.07103534680971733
100	0.07050494955353812
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2639531.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.58870532542711
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.30105337764977	49.7906260990191
2	12.985856655634489	16.51507624546756
3	3.89154655874166	7.4237522215200995
4	1.6733199558178007	4.256169983426194
5	0.8396662485597358	2.669664482568594
6	0.5376537143322523	2.0513222164676974
7	0.35482793645872707	1.579413436489236
8	0.2517891311913724	1.2808755893978792
9	0.18046964497899792	1.0328247967278552
>10	0.928586295169705	10.369587510050467
>50	0.04323252262519341	1.853898851647904
>100	0.011819076940413335	1.0493900408996297
>500	5.9627299969568435E-5	0.02282556929883673
>1k	1.1925459993769096E-4	0.10457295701898411
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	1.8942759149257955E-5	0.0
12-13	0.0	0.0	0.0	3.788551829851591E-5	0.0
14-15	0.0	0.0	0.0	5.682827744777387E-5	0.0
16-17	0.0	0.0	0.0	7.577103659703182E-5	0.0
18-19	0.0	0.0	0.0	1.1365655489554773E-4	0.0
20-21	0.0	0.0	0.0	1.3259931404480569E-4	0.0
22-23	0.0	0.0	0.0	1.704848323433216E-4	0.0
24-25	0.0	0.0	0.0	1.8942759149257954E-4	0.0
26-27	0.0	0.0	0.0	2.8414138723886935E-4	0.0
28-29	0.0	0.0	0.0	0.001269164863000283	0.0
30-31	5.682827744777387E-5	0.0	0.0	0.00564494222647887	0.0
32-33	7.577103659703182E-5	0.0	0.0	0.014169183843644951	0.0
34-35	7.577103659703182E-5	0.0	0.0	0.02545906829660269	0.0
36-37	7.577103659703182E-5	0.0	0.0	0.04845557790380185	0.0
38-39	7.577103659703182E-5	0.0	0.0	0.08814065832149727	0.0
40-41	7.577103659703182E-5	0.0	0.0	0.13511870101165702	0.0
42-43	7.577103659703182E-5	0.0	0.0	0.18027823882348795	0.0
44-45	7.577103659703182E-5	0.0	0.0	0.23790211215553064	0.0
46-47	7.577103659703182E-5	0.0	0.0	0.30778195065714326	0.0
48-49	7.577103659703182E-5	0.0	0.0	0.3888190742976688	0.0
50-51	7.577103659703182E-5	0.0	0.0	0.4783236112779127	0.0
52-53	7.577103659703182E-5	0.0	0.0	0.5682259462002909	0.0
54-55	9.471379574628978E-5	0.0	0.0	0.663185997815521	0.0
56-57	1.1365655489554773E-4	0.0	0.0	0.7781874886106661	0.0
58-59	1.1365655489554773E-4	0.0	0.0	0.9123401089057109	0.0
60-61	1.1365655489554773E-4	0.0	0.0	1.0585024385013853	0.0
62-63	1.1365655489554773E-4	0.0	0.0	1.2076766668017918	0.0
64-65	1.5154207319406364E-4	0.0	0.0	1.363026234584856	0.0
66-67	1.5154207319406364E-4	0.0	0.0	1.5367881642609995	0.0
68-69	1.5154207319406364E-4	0.0	0.0	1.7377140105571787	0.0
70-71	1.5154207319406364E-4	0.0	0.0	1.9612953967958702	0.0
72-73	1.5154207319406364E-4	0.0	0.0	2.1943481626091907	0.0
74-75	1.5154207319406364E-4	0.0	0.0	2.4235176627969137	0.0
76-77	1.5154207319406364E-4	0.0	0.0	2.6687316799840577	0.0
78-79	1.5154207319406364E-4	0.0	0.0	2.951433417527584	0.0
80-81	1.5154207319406364E-4	0.0	0.0	3.261374842727742	0.0
82-83	1.5154207319406364E-4	0.0	0.0	3.584386771740889	0.0
84-85	1.5154207319406364E-4	0.0	0.0	3.8999731391675265	0.0
86-87	1.5154207319406364E-4	0.0	0.0	4.230637942876974	0.0
88	1.5154207319406364E-4	0.0	0.0	4.501178429046675	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2605	0.0	37.709	1
GTATCAA	4250	0.0	32.955948	1
TCAACGC	4190	0.0	32.08178	4
ATCAACG	4215	0.0	32.003006	3
CAACGCA	4315	0.0	31.151823	5
AACGCAG	4570	0.0	29.516436	6
GTACATG	9065	0.0	28.20574	1
TATCAAC	5000	0.0	27.82455	2
TACATGG	9110	0.0	26.725008	2
ACATGGG	9210	0.0	25.36315	3
ACGCAGA	5530	0.0	24.052004	7
CGCAGAG	5810	0.0	22.488405	8
CATGGGG	6405	0.0	21.060524	4
TACCTGG	1740	0.0	19.718779	2
GCAGAGT	6600	0.0	19.297768	9
GTATAGA	1140	0.0	18.552998	1
GAGTACA	6380	0.0	18.196285	1
GTACCGC	310	6.373739E-9	18.193563	6
TACCGCT	310	1.2616329E-7	16.677116	7
CATGGGA	3165	0.0	16.48374	4
>>END_MODULE