FastQCFastQC Report
Wed 25 May 2016
SRR1032952_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032952_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2639531
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56290.21325758250234605No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49560.18776062868744484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42650.046.9243281
GTATCAA75250.039.8310471
ATCAACG87000.033.7905463
TCAACGC86750.033.7794844
CAACGCA89850.032.6096955
TATCAAC93000.031.7616392
AACGCAG93500.031.4384926
ACGCAGA109050.026.9108547
CGCAGAG111650.025.9893258
GTACATG83600.025.7937151
TACATGG84800.024.4607032
GCAGAGT119950.023.8884379
GTGGTAT20750.023.772711
ACATGGG88200.022.824943
GTACCGT2756.002665E-1122.2332976
TGGTATC23150.021.943152
TAGACCG1100.003049201621.3773615
GAGTACT77200.020.50474212-13
AGAGTAC109250.020.10064910-11
CATGGGG58300.019.927964
Produced by FastQC (version 0.11.5)