FastQCFastQC Report
Wed 25 May 2016
SRR1032951_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032951_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2842700
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG87800.028.0508881
TACATGG87350.026.6887722
ACATGGG89900.024.5201423
GTATCAA52950.024.4992561
TCAACGC51050.024.2141844
TAGACCG1000.001759032123.5006485
ATCAACG52700.023.4560533
CAACGCA53000.023.4119645
AACGCAG55100.022.4339796
GGTATCA34900.021.9518931
CATGGGG60500.020.8980964
TATCAAC61050.020.6328372
GAGTACA64400.019.7055111
ACGCAGA63850.018.8443537
GTATAGG13650.017.9052561
AGTACAT65100.017.9052542
CGCAGAG66450.017.4701448
GTCGTAC1700.001919220116.5886921
TACACCG4201.0022632E-915.6670985
TAGACTG11450.015.5986835
Produced by FastQC (version 0.11.5)