FastQCFastQC Report
Wed 25 May 2016
SRR1032951_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032951_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2842700
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46790.16459703802722764No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39550.13912829352376263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53000.039.0890851
GTATCAA89600.036.8062741
TCAACGC100900.032.0360374
ATCAACG102150.031.6900773
CAACGCA102900.031.5494375
AACGCAG105650.030.772226
TATCAAC108350.030.0069852
GTACATG86850.027.3158231
ACGCAGA121850.026.6791617
CGCAGAG123700.026.098
TACATGG87750.025.3622192
GCAGAGT135150.023.7379889
ACATGGG90350.023.642913
GAGTACT77050.021.3671712-13
AGAGTAC110600.020.72508410-11
TAGACCG1150.00393582720.4564425
TATAGCG1456.556252E-419.468895
GTACTTT85050.019.21925414-15
CAGAGTA132550.019.06488610-11
CATGGGG59700.018.9151654
Produced by FastQC (version 0.11.5)