FastQCFastQC Report
Wed 25 May 2016
SRR1032950_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032950_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2945583
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG85150.028.2610761
TACATGG85750.026.8026712
TCAACGC46150.025.460784
GTATCAA47200.025.2926921
CAACGCA47100.024.9472375
ATCAACG48000.024.8711473
ACATGGG89200.024.448743
GGTATCA33700.023.70951
AACGCAG49750.023.618396
TATCAAC55350.021.7383062
CATGGGG58650.021.5569654
GAGTACA63150.020.244121
CGCAGAG58300.019.9933918
ACGCAGA59600.019.6361547
AGTACAT61650.018.9832082
TACACCG4850.018.4126035
GTCTAGG8350.018.0122031
TACCGCG1952.468183E-416.8720078
GCAGAGT69700.016.0489859
GTATAGA12150.015.8602831
Produced by FastQC (version 0.11.5)