##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1032950_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2945583
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.413377928919335	33.0	31.0	34.0	30.0	34.0
2	31.532224350833093	33.0	31.0	34.0	30.0	34.0
3	31.613813292648686	34.0	31.0	34.0	30.0	34.0
4	35.00210586495101	37.0	35.0	37.0	33.0	37.0
5	35.062392062963426	37.0	35.0	37.0	33.0	37.0
6	35.19429566235275	37.0	35.0	37.0	33.0	37.0
7	35.15388532592699	37.0	35.0	37.0	33.0	37.0
8	35.172820456935014	37.0	36.0	37.0	33.0	37.0
9	36.83157357983122	39.0	38.0	39.0	34.0	39.0
10-11	36.813866728589886	39.0	38.0	39.0	34.0	39.0
12-13	36.75838637037218	39.0	38.0	39.0	33.0	39.0
14-15	38.21872274520867	41.0	39.0	41.0	34.0	41.0
16-17	38.23720838964647	41.0	39.0	41.0	34.0	41.0
18-19	38.24768780917054	41.0	39.0	41.0	34.0	41.0
20-21	38.2764748099103	41.0	39.0	41.0	34.0	41.0
22-23	38.2677191578034	41.0	39.0	41.0	34.0	41.0
24-25	38.29690557013671	41.0	39.0	41.0	34.0	41.0
26-27	38.265766573204694	41.0	39.0	41.0	34.0	41.0
28-29	38.245796672509314	41.0	39.0	41.0	34.0	41.0
30-31	38.200806088302386	41.0	39.0	41.0	33.0	41.0
32-33	38.15021270831615	41.0	39.0	41.0	33.0	41.0
34-35	38.08878751676663	41.0	38.5	41.0	33.0	41.0
36-37	38.00462319343913	41.0	38.0	41.0	33.0	41.0
38-39	37.919279137610445	40.5	38.0	41.0	33.0	41.0
40-41	37.83976652499692	40.0	38.0	41.0	33.0	41.0
42-43	37.739972528358564	40.0	38.0	41.0	32.5	41.0
44-45	37.61912225864965	40.0	38.0	41.0	32.0	41.0
46-47	37.50474693804249	40.0	38.0	41.0	32.0	41.0
48-49	37.38089369744462	40.0	38.0	41.0	31.5	41.0
50-51	37.25641749018785	40.0	37.5	41.0	31.0	41.0
52-53	37.10534366201869	40.0	37.0	41.0	31.0	41.0
54-55	36.95299368580006	40.0	37.0	41.0	31.0	41.0
56-57	36.77525399895369	40.0	36.0	41.0	30.5	41.0
58-59	36.57723581375911	40.0	36.0	41.0	30.0	41.0
60-61	36.34745006336607	39.0	35.0	41.0	30.0	41.0
62-63	36.1044283932926	39.0	35.0	41.0	30.0	41.0
64-65	35.83445569179344	39.0	35.0	41.0	29.5	41.0
66-67	35.54682774174077	38.0	35.0	40.0	29.0	41.0
68-69	35.234394685194744	37.5	35.0	40.0	29.0	41.0
70-71	34.89616367965187	37.0	34.5	40.0	29.0	41.0
72-73	34.60718574217735	36.5	34.0	39.0	29.0	41.0
74-75	34.32774887008786	36.0	34.0	39.0	29.0	41.0
76-77	33.991833874652315	35.5	34.0	39.0	28.5	40.0
78-79	33.609069919265565	35.0	34.0	37.5	28.0	39.5
80-81	33.27575440922901	35.0	34.0	37.0	28.0	39.0
82-83	32.946036658956814	35.0	34.0	36.5	27.0	39.0
84-85	32.76616819149214	35.0	34.0	36.0	27.0	37.5
86-87	32.52276883727262	35.0	34.0	36.0	27.0	37.0
88-89	32.279261694544005	35.0	34.0	35.5	27.0	37.0
90-91	32.0735825471562	35.0	33.5	35.0	26.0	36.0
92-93	31.877778524658787	35.0	33.0	35.0	26.0	36.0
94-95	31.694635662957044	35.0	33.0	35.0	25.5	36.0
96-97	31.521995815429406	35.0	33.0	35.0	25.0	36.0
98-99	31.344086043408048	35.0	33.0	35.0	25.0	35.5
100	31.215071515553966	35.0	33.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.35317680678236485
1101	2	0.3737370904346271
1101	3	0.4449834353457902
1101	4	0.39142649712229627
1101	5	0.3749168011335371
1101	6	0.42339997520473815
1101	7	0.42350064168535795
1101	8	0.37398012988298035
1101	9	0.5176044834793316
1101	10-11	0.5460254187749669
1101	12-13	0.3706086236188355
1101	14-15	0.42757364969728684
1101	16-17	0.46636011215606743
1101	18-19	0.4902437003622566
1101	20-21	0.47030318360643264
1101	22-23	0.45071688544948074
1101	24-25	0.43470836044099315
1101	26-27	0.494533198606419
1101	28-29	0.45888289360588175
1101	30-31	0.4230745820816324
1101	32-33	0.3967157344756842
1101	34-35	0.4227877995140261
1101	36-37	0.4001878751919108
1101	38-39	0.46341193122198376
1101	40-41	0.4287709036768348
1101	42-43	0.5086759296261292
1101	44-45	0.4362804563704259
1101	46-47	0.41356839319973204
1101	48-49	0.42518136604745393
1101	50-51	0.4327746623178115
1101	52-53	0.4301049781277939
1101	54-55	0.4637004578128483
1101	56-57	0.49487255660854146
1101	58-59	0.514655744879498
1101	60-61	0.5933826156636286
1101	62-63	0.5335288796989275
1101	64-65	0.4167382986477861
1101	66-67	0.40881405388728354
1101	68-69	0.4193784305831798
1101	70-71	0.45019733678107343
1101	72-73	0.41870623713953137
1101	74-75	0.45579851800135174
1101	76-77	0.38346333031141455
1101	78-79	0.39550669048323783
1101	80-81	0.3880797811008705
1101	82-83	0.3866280851026076
1101	84-85	0.4674347239240504
1101	86-87	0.429523143257839
1101	88-89	0.3865163362926509
1101	90-91	0.4020121618309602
1101	92-93	0.37564816023644454
1101	94-95	0.40881921595908466
1101	96-97	0.44080431783925533
1101	98-99	0.43111306778333613
1101	100	0.411252789561086
1102	1	0.38320702333432166
1102	2	0.33063653646450675
1102	3	0.34974506032481045
1102	4	0.34033954060056004
1102	5	0.309124875667699
1102	6	0.3835506382901812
1102	7	0.4064538899646948
1102	8	0.48877239108828974
1102	9	0.49700518853221354
1102	10-11	0.4707710961293401
1102	12-13	0.49579265081373336
1102	14-15	0.5971904870315186
1102	16-17	0.5273387926999646
1102	18-19	0.5020080112570042
1102	20-21	0.48173993868449116
1102	22-23	0.5263793413836737
1102	24-25	0.5605221089721368
1102	26-27	0.5779785150408685
1102	28-29	0.5700445521588406
1102	30-31	0.5930571655968251
1102	32-33	0.5869157092952264
1102	34-35	0.607535994914393
1102	36-37	0.6235689814534524
1102	38-39	0.6178015149050466
1102	40-41	0.6718106048687105
1102	42-43	0.7003470728190209
1102	44-45	0.6378666155109372
1102	46-47	0.6530412822378366
1102	48-49	0.6505152169791373
1102	50-51	0.6550069100935403
1102	52-53	0.5765027034929062
1102	54-55	0.5899561234163428
1102	56-57	0.6374560718007558
1102	58-59	0.6691578012837383
1102	60-61	0.635327176684271
1102	62-63	0.6405554870516212
1102	64-65	0.6779295442412092
1102	66-67	0.6490866323663909
1102	68-69	0.6051048868531126
1102	70-71	0.607727343495867
1102	72-73	0.6219070932751691
1102	74-75	0.5918539989481317
1102	76-77	0.5338987424316102
1102	78-79	0.5977834237382353
1102	80-81	0.5284543404428206
1102	82-83	0.49074131323757086
1102	84-85	0.5129454675869738
1102	86-87	0.5515531080051943
1102	88-89	0.5478702056899962
1102	90-91	0.5331696236405961
1102	92-93	0.5754639232043885
1102	94-95	0.5664979972248219
1102	96-97	0.5376336782555704
1102	98-99	0.47136739042895925
1102	100	0.52451323945197
1103	1	0.1867540675868007
1103	2	0.24995756660536372
1103	3	0.3279928675764303
1103	4	0.26085908302191285
1103	5	0.17664326052402402
1103	6	0.23587805252179095
1103	7	0.279224105025186
1103	8	0.24610033901442563
1103	9	0.34027842908857764
1103	10-11	0.3491752390711369
1103	12-13	0.33114708379090274
1103	14-15	0.36217235061919695
1103	16-17	0.38329371943659396
1103	18-19	0.3719176696556943
1103	20-21	0.3392907730882797
1103	22-23	0.37636482581267217
1103	24-25	0.37808208519512476
1103	26-27	0.423865363519468
1103	28-29	0.4038571370438788
1103	30-31	0.40911118008387604
1103	32-33	0.4099491599800089
1103	34-35	0.3460670835408166
1103	36-37	0.3472036666288929
1103	38-39	0.34954030547962844
1103	40-41	0.3940044298389864
1103	42-43	0.36584436569952317
1103	44-45	0.39722823864162393
1103	46-47	0.39099634883923073
1103	48-49	0.4237643998744076
1103	50-51	0.34301852071001093
1103	52-53	0.3894939555034682
1103	54-55	0.3706302164116906
1103	56-57	0.3206922665955716
1103	58-59	0.38640908583125366
1103	60-61	0.37832370122870174
1103	62-63	0.4239990405738112
1103	64-65	0.33929035946879793
1103	66-67	0.3455523553752471
1103	68-69	0.47152137654176585
1103	70-71	0.38051621486076215
1103	72-73	0.43820460589060417
1103	74-75	0.3338750357141649
1103	76-77	0.35134519354702576
1103	78-79	0.29093460467539956
1103	80-81	0.20733597895863198
1103	82-83	0.22206085455472646
1103	84-85	0.22099454723996104
1103	86-87	0.26395501863160575
1103	88-89	0.19557062638850908
1103	90-91	0.15795384542195023
1103	92-93	0.1833316387341597
1103	94-95	0.19230843076881143
1103	96-97	0.18018417679987309
1103	98-99	0.19753562934743485
1103	100	0.19331828820220665
1104	1	0.10966680596150624
1104	2	0.1035975584981017
1104	3	0.15947502728292307
1104	4	0.10955470405986745
1104	5	0.19692877418049903
1104	6	0.25282699995872804
1104	7	0.21487434504931002
1104	8	0.159345362539419
1104	9	0.18914921391296247
1104	10-11	0.2713848910046721
1104	12-13	0.28691330986999475
1104	14-15	0.30291913849109875
1104	16-17	0.30757466933046373
1104	18-19	0.2959062286423588
1104	20-21	0.2899756745445643
1104	22-23	0.3316777534305757
1104	24-25	0.32998429208201685
1104	26-27	0.29881875448040063
1104	28-29	0.29536966694604416
1104	30-31	0.29489546737281813
1104	32-33	0.33212372389954226
1104	34-35	0.33335872980853765
1104	36-37	0.3504020910610208
1104	38-39	0.3191629540577523
1104	40-41	0.33577587060478464
1104	42-43	0.2980485759517748
1104	44-45	0.28562674874376626
1104	46-47	0.2759819831984771
1104	48-49	0.3458952557324082
1104	50-51	0.33163323376078324
1104	52-53	0.33618545416945267
1104	54-55	0.28403277683104733
1104	56-57	0.27289938003561787
1104	58-59	0.30016423934877423
1104	60-61	0.3496776532000112
1104	62-63	0.29687237538508526
1104	64-65	0.28110075621864183
1104	66-67	0.3214615604145692
1104	68-69	0.2929837808675302
1104	70-71	0.3558775495712325
1104	72-73	0.346367835110712
1104	74-75	0.292889444897213
1104	76-77	0.2535004706627717
1104	78-79	0.21582832390984663
1104	80-81	0.2979382042128549
1104	82-83	0.3249934776108816
1104	84-85	0.26944681217955235
1104	86-87	0.29306710179429274
1104	88-89	0.3183178377478768
1104	90-91	0.26730072320758325
1104	92-93	0.25118083409692815
1104	94-95	0.2883103974658141
1104	96-97	0.31078140574192403
1104	98-99	0.37474236704182573
1104	100	0.3245931026114768
1105	1	-0.007869811787688974
1105	2	0.04433622911697199
1105	3	-0.02845592593572377
1105	4	0.07029048744062294
1105	5	0.0513044819386792
1105	6	0.04487951997327144
1105	7	0.07589953464134425
1105	8	0.13795800600799168
1105	9	0.18872279349417198
1105	10-11	0.13854924388695622
1105	12-13	0.1777310885914858
1105	14-15	0.23352348694254488
1105	16-17	0.26406312336680315
1105	18-19	0.2700680924587715
1105	20-21	0.17812680479671883
1105	22-23	0.15751029816914297
1105	24-25	0.20617496702232074
1105	26-27	0.18928290207677634
1105	28-29	0.1763620019054457
1105	30-31	0.1891139238070778
1105	32-33	0.269398105522491
1105	34-35	0.2637030522621444
1105	36-37	0.22989851610690692
1105	38-39	0.17934548590744015
1105	40-41	0.19528962436928765
1105	42-43	0.23193751483039904
1105	44-45	0.29733654121545783
1105	46-47	0.2526429372809744
1105	48-49	0.2128838245864486
1105	50-51	0.20303695465849358
1105	52-53	0.210378338074392
1105	54-55	0.23888963451617684
1105	56-57	0.2655287763621601
1105	58-59	0.26428779303846994
1105	60-61	0.14003332570510452
1105	62-63	0.19491835314446604
1105	64-65	0.17314587563566874
1105	66-67	0.1751991980708425
1105	68-69	0.20725031843899444
1105	70-71	0.21976723062558534
1105	72-73	0.17620807583590192
1105	74-75	0.17690658233234302
1105	76-77	0.15514853982360677
1105	78-79	0.2075431724866874
1105	80-81	0.1586552824379055
1105	82-83	0.1932585305135106
1105	84-85	0.15384067123884648
1105	86-87	0.13271231429274621
1105	88-89	0.18054838841951693
1105	90-91	0.18983527286270885
1105	92-93	0.2015234859939241
1105	94-95	0.18821932621001025
1105	96-97	0.1190558344980559
1105	98-99	0.1800559484501676
1105	100	0.2263074544648731
1106	1	0.0496402548908037
1106	2	0.10118311330688812
1106	3	0.05794506341155525
1106	4	0.1321172443536156
1106	5	0.09743465327627376
1106	6	0.1485079018733586
1106	7	0.15728082687591183
1106	8	0.16847975263275572
1106	9	0.2024257275636785
1106	10-11	0.21264138080442763
1106	12-13	0.2524008311602586
1106	14-15	0.20942395515272239
1106	16-17	0.2376844052774274
1106	18-19	0.22862655899480444
1106	20-21	0.20103692494933512
1106	22-23	0.19825422080768362
1106	24-25	0.2137294409352961
1106	26-27	0.25759807047238326
1106	28-29	0.19741587989659592
1106	30-31	0.16431047313011504
1106	32-33	0.15677708763844578
1106	34-35	0.16957217928569435
1106	36-37	0.24426090862855432
1106	38-39	0.23918201134799943
1106	40-41	0.30673893878938685
1106	42-43	0.33491191033188983
1106	44-45	0.22592548252966083
1106	46-47	0.22226840409653192
1106	48-49	0.2802630438247249
1106	50-51	0.26894130346664014
1106	52-53	0.2427925032145879
1106	54-55	0.2456017161871884
1106	56-57	0.27886994689217204
1106	58-59	0.263658962885998
1106	60-61	0.30553087293310455
1106	62-63	0.19768690823200785
1106	64-65	0.22107224854730134
1106	66-67	0.2518638081573812
1106	68-69	0.2600810953353161
1106	70-71	0.2435442152596039
1106	72-73	0.1717891646012788
1106	74-75	0.14214217019733155
1106	76-77	0.1541237457970155
1106	78-79	0.22713943221648947
1106	80-81	0.22584138378672236
1106	82-83	0.1990935636034763
1106	84-85	0.15916057128875138
1106	86-87	0.14566170098953535
1106	88-89	0.1806944618486952
1106	90-91	0.16761827949164854
1106	92-93	0.1613577010118874
1106	94-95	0.18161060555028996
1106	96-97	0.18897910262731088
1106	98-99	0.22804965802062327
1106	100	0.21628645241938926
1107	1	0.0499769088542088
1107	2	0.11023700356497557
1107	3	0.12510483568458497
1107	4	0.17141471542573328
1107	5	0.12128146907429027
1107	6	0.10977289576243976
1107	7	0.13736655837736578
1107	8	0.09406743388333183
1107	9	0.07654136181838567
1107	10-11	0.024851728334972734
1107	12-13	0.08789353978961856
1107	14-15	0.1298660040820323
1107	16-17	0.1058232305594089
1107	18-19	0.18620852358251483
1107	20-21	0.19064083508538943
1107	22-23	0.14994580870013863
1107	24-25	0.17249459594462735
1107	26-27	0.21325540031775603
1107	28-29	0.13257281781210395
1107	30-31	0.15923127864594022
1107	32-33	0.12405635581087182
1107	34-35	0.08724041211880973
1107	36-37	0.1810777733372433
1107	38-39	0.14644769980123584
1107	40-41	0.16233190789001384
1107	42-43	0.1634249577719018
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2216	72-73	-0.39087066502646195
2216	74-75	-0.36409162990863564
2216	76-77	-0.37395162239820223
2216	78-79	-0.3674885659037841
2216	80-81	-0.31612161777704273
2216	82-83	-0.2990448603350728
2216	84-85	-0.3343471915425553
2216	86-87	-0.24882522271133212
2216	88-89	-0.2887389608263291
2216	90-91	-0.3025954160369153
2216	92-93	-0.3422785653272271
2216	94-95	-0.27493648860621533
2216	96-97	-0.3215790720380802
2216	98-99	-0.39816291975121487
2216	100	-0.395178855140653
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	42888.0
3	6352.0
4	4105.0
5	3449.0
6	2754.0
7	3275.0
8	3681.0
9	4033.0
10	4349.0
11	4737.0
12	5169.0
13	5684.0
14	6082.0
15	6181.0
16	6140.0
17	6230.0
18	6150.0
19	6066.0
20	6015.0
21	6272.0
22	7573.0
23	10318.0
24	15392.0
25	20399.0
26	20906.0
27	18897.0
28	20515.0
29	25483.0
30	31048.0
31	38110.0
32	48732.0
33	64431.0
34	93056.0
35	144132.0
36	268423.0
37	574701.0
38	964501.0
39	438570.0
40	784.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.26015837266262	19.73073861375845	13.08624878580619	23.922854227772735
2	15.328199312187039	19.996996509291453	40.77846809742454	23.896336081096965
3	18.444259536294304	25.172133896302768	29.7586676827932	26.624938884609726
4	12.085806607251463	16.474229816555845	39.2135923319731	32.226371244219585
5	12.812433320603372	38.349571960744605	35.84966369800366	12.988331020648358
6	31.159923538849295	38.246646330483145	17.74361459013575	12.849815540531818
7	27.630069233672906	32.498341103165544	22.333253262327226	17.53833640083432
8	25.04157859705404	37.02786844752738	20.22260645734724	17.70794649807133
9	24.893849578133402	17.023717828276357	21.037982433578904	37.04445016001133
10-11	23.811923179047806	27.179223123139906	28.95560274099897	20.053250956813315
12-13	24.300655138115822	24.45891435468513	29.05074198872174	22.189688518477308
14-15	21.507697628757295	26.637536188011516	25.876793771907742	25.977972411323446
16-17	21.371814993265307	30.019316480945214	26.506285458211355	22.102583067578124
18-19	21.306012148713187	29.043979374571283	28.388690192864814	21.26131828385072
20-21	22.442527178459926	28.34613527115777	27.85379137753517	21.35754617284714
22-23	22.032804474406095	28.074948068812137	28.01598400172866	21.876263455053106
24-25	21.92121645048487	28.13079119055284	27.96764195503883	21.980350403923463
26-27	21.756244996112407	28.072852016750378	28.055830517509634	22.11507246962758
28-29	21.718220355178143	28.209678939952315	28.094898762592567	21.97720194227697
30-31	21.935736167215683	28.57480671704456	27.750949320802228	21.738507794937533
32-33	21.637418158480447	28.27546995064452	28.227684118150087	21.859427772724942
34-35	21.75792176765533	28.039516556310517	28.267397380160418	21.935164295873737
36-37	22.019398200216372	28.4272183597838	27.755828437410234	21.797555002589586
38-39	21.840466349047322	28.5803223790501	27.88743045812913	21.69178081377345
40-41	21.655104324382265	28.256995374213727	28.17156671139398	21.91633359001003
42-43	21.64874231695274	28.1577555691422	28.102816291992916	22.090685821912142
44-45	21.72993390054724	28.35447205463818	28.11867560541333	21.796918439401246
46-47	21.792149245140635	28.457958548226365	28.010849669960326	21.739042536672677
48-49	21.65596763191583	28.273212318288426	28.07730827745965	21.993511772336095
50-51	21.645974530629776	28.249369857688755	28.059511666042585	22.045143945638884
52-53	21.755540185618326	28.18639780942716	28.088503655060553	21.96955834989396
54-55	21.744180040883784	28.59367524573765	27.878344913448156	21.78379979993041
56-57	21.669184464541523	28.228319755573132	28.01199640644665	22.090499373438693
58-59	21.570730421126417	27.876855664621083	28.349829073797782	22.20258484045472
60-61	21.885911420732917	28.27869903798994	27.935139792750004	21.900249748527138
62-63	21.66609613040505	28.250514969144326	28.01184110494328	22.071547795507346
64-65	21.591902726437045	28.071902903117685	28.165084950258457	22.171109420186816
66-67	21.488465363472624	28.15476054973211	27.988953441160053	22.367820645635213
68-69	21.869940121103273	28.211102883078752	27.95186040455078	21.9670965912672
70-71	21.82533750197911	28.00228455560229	28.00439107623498	22.167986866183618
72-73	21.57243145134712	28.069408590131005	28.149133709200548	22.209026249321326
74-75	21.590265478006167	28.184966518384226	28.040415770692128	22.18435223291748
76-77	21.650966690546056	28.128368391073376	27.893883779009254	22.326781139371313
78-79	21.727724810001472	28.15110796870055	28.061682906953255	22.059484314344722
80-81	21.66524191937244	27.956056172644033	28.122337092944715	22.256364815038808
82-83	21.499386473468522	27.995819559494233	28.290665647831524	22.214128319205717
84-85	21.47660971455995	27.926527110646777	28.175451560980374	22.421411613812904
86-87	21.713961386280406	28.037425568555076	28.050251619947282	22.19836142521724
88-89	21.43676797025938	27.914006471275837	28.151986476470658	22.497239081994124
90-91	21.469725335739174	27.800717097459106	28.178155588099248	22.551401978702472
92-93	21.68267456096139	27.940133006431527	28.07032295907629	22.30686947353079
94-95	21.407537605042624	27.664832556082896	28.28836560311355	22.639264235760926
96-97	21.422685403422047	27.663383912380613	28.336768971389503	22.57716171280784
98-99	21.45103674238171	27.805178760196664	28.123600164638052	22.620184332783577
100	21.544865842940247	27.61236547687359	28.052902492412823	22.789866187773338
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2352.0
1	1325.0
2	273.0
3	221.0
4	180.5
5	151.0
6	140.5
7	140.0
8	161.5
9	181.0
10	174.0
11	179.5
12	191.0
13	219.0
14	257.5
15	312.5
16	412.0
17	562.5
18	783.5
19	1157.0
20	1702.0
21	2452.5
22	3758.5
23	5957.5
24	8952.5
25	12531.5
26	17285.0
27	23497.5
28	31487.0
29	40552.0
30	49083.5
31	57877.5
32	66771.0
33	74958.5
34	83000.5
35	90141.5
36	95262.0
37	99565.0
38	103407.0
39	105238.5
40	106733.0
41	107944.0
42	107146.5
43	105785.5
44	104514.0
45	103378.5
46	102100.0
47	100011.5
48	98674.5
49	95759.5
50	91253.0
51	87584.0
52	81983.5
53	77413.0
54	74371.5
55	70208.5
56	64749.5
57	58321.0
58	52625.0
59	47753.0
60	42365.5
61	36390.5
62	31320.5
63	26259.0
64	21257.0
65	16966.5
66	13097.0
67	9851.5
68	7432.5
69	5664.0
70	4121.5
71	2962.5
72	2216.0
73	1617.0
74	1187.0
75	865.5
76	576.0
77	415.0
78	307.5
79	195.5
80	114.5
81	67.0
82	59.0
83	51.5
84	45.0
85	38.5
86	27.5
87	23.0
88	18.0
89	16.5
90	16.0
91	12.0
92	8.0
93	5.5
94	5.0
95	4.5
96	3.5
97	3.5
98	3.0
99	1.5
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0771324386377841
2	0.07947492907176609
3	0.08079894540401679
4	0.0832772323848963
5	0.08130818245488246
6	0.08110448763453619
7	0.08018786094297801
8	0.08018786094297801
9	0.079916267849183
10-11	0.0797634967339233
12-13	0.0784394804016726
14-15	0.07720033691123285
16-17	0.07609698996769061
18-19	0.07557077834846276
20-21	0.07514641413940805
22-23	0.07689479468071346
24-25	0.07691176924907565
26-27	0.07626673565131249
28-29	0.07232863579128479
30-31	0.07789629421408258
32-33	0.07611396453605279
34-35	0.07327921161956734
36-37	0.07002009449402716
38-39	0.07205704269748976
40-41	0.07439953313147177
42-43	0.07922031054633327
44-45	0.0803406320582377
46-47	0.08324328324817193
48-49	0.08692676458276681
50-51	0.08816590807320655
52-53	0.0885563231455369
54-55	0.08850539944045033
56-57	0.0860610615962952
58-59	0.08487284181094201
60-61	0.08224178371480281
62-63	0.08302261385946348
64-65	0.08213993630462968
66-67	0.08161372468540183
68-69	0.0812233096130715
70-71	0.07947492907176609
72-73	0.0796107256186636
74-75	0.07896569202090045
76-77	0.07874502263219199
78-79	0.0793221579565064
80-81	0.0804934031734974
82-83	0.07986534414409642
84-85	0.07877897176891638
86-87	0.07993324241754518
88-89	0.07701361665924877
90-91	0.07433163485802302
92-93	0.07439953313147177
94-95	0.0745013805416449
96-97	0.07368660126025985
98-99	0.07321131334611858
100	0.0723116612229226
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2945583.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.7080161302818
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.389153696613	49.42990589579011
2	14.411337144063038	18.65058072753921
3	4.506420759686454	8.748046416228835
4	1.862562899063997	4.820910004651903
5	0.8882782631687829	2.873936209065216
6	0.5048648646670593	1.960128228389317
7	0.3202562368709808	1.4506202018889545
8	0.21811121875736114	1.12908354092374
9	0.15178835789799033	0.8839731160126931
>10	0.7086506538239051	7.826357769591101
>50	0.030779885039871117	1.340798824766766
>100	0.007586000125114618	0.743439799507025
>500	1.5751516684657597E-4	0.07501675551648866
>1k	5.2505055593361626E-5	0.06720251012859531
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	6.789827344875361E-5	0.0	0.0	0.0
6	0.0	6.789827344875361E-5	0.0	0.0	0.0
7	0.0	6.789827344875361E-5	0.0	0.0	0.0
8	0.0	6.789827344875361E-5	0.0	0.0	0.0
9	0.0	6.789827344875361E-5	0.0	0.0	0.0
10-11	0.0	6.789827344875361E-5	0.0	0.0	0.0
12-13	0.0	6.789827344875361E-5	0.0	0.0	0.0
14-15	0.0	6.789827344875361E-5	0.0	3.3949136724376805E-5	0.0
16-17	0.0	6.789827344875361E-5	0.0	8.487284181094201E-5	0.0
18-19	0.0	6.789827344875361E-5	0.0	1.3579654689750722E-4	0.0
20-21	0.0	6.789827344875361E-5	0.0	1.3579654689750722E-4	0.0
22-23	0.0	8.487284181094201E-5	0.0	1.8672025198407242E-4	0.0
24-25	0.0	1.0184741017313041E-4	0.0	2.7159309379501444E-4	0.0
26-27	0.0	1.0184741017313041E-4	0.0	3.7344050396814483E-4	0.0
28-29	0.0	1.0184741017313041E-4	0.0	0.001222168922077565	0.0
30-31	0.0	1.0184741017313041E-4	0.0	0.005228167055554028	0.0
32-33	0.0	1.0184741017313041E-4	0.0	0.013206214185782578	0.0
34-35	0.0	1.0184741017313041E-4	0.0	0.02541092883819604	0.0
36-37	0.0	1.0184741017313041E-4	0.0	0.04739299486723002	0.0
38-39	0.0	1.0184741017313041E-4	0.0	0.08707953569802651	0.0
40-41	0.0	1.0184741017313041E-4	0.0	0.1372903089133798	0.0
42-43	0.0	1.0184741017313041E-4	0.0	0.1882479631366694	0.0
44-45	0.0	1.0184741017313041E-4	0.0	0.24759105413088003	0.0
46-47	0.0	1.0184741017313041E-4	0.0	0.32139647736967525	0.0
48-49	0.0	1.0184741017313041E-4	0.0	0.4085778604778748	0.0
50-51	0.0	1.0184741017313041E-4	0.0	0.5072680009356383	0.0
52-53	0.0	1.3579654689750722E-4	0.0	0.6039551423266634	0.0
54-55	0.0	1.3579654689750722E-4	0.0	0.7081280683654136	0.0
56-57	0.0	1.3579654689750722E-4	0.0	0.8326365273020655	0.0
58-59	0.0	1.3579654689750722E-4	0.0	0.9787875609005077	0.0
60-61	0.0	1.3579654689750722E-4	0.0	1.1403005788667302	0.0
62-63	0.0	1.3579654689750722E-4	0.0	1.3069059673416095	0.0
64-65	0.0	1.3579654689750722E-4	0.0	1.4769911423307374	0.0
66-67	0.0	1.3579654689750722E-4	0.0	1.6669535368719877	0.0
68-69	0.0	1.3579654689750722E-4	0.0	1.8876738492855236	0.0
70-71	0.0	1.3579654689750722E-4	0.0	2.13166629492362	0.0
72-73	0.0	2.0369482034626082E-4	0.0	2.383789558807204	0.0
74-75	0.0	2.0369482034626082E-4	0.0	2.6311938926861	0.0
76-77	0.0	2.0369482034626082E-4	0.0	2.8945543208254527	0.0
78-79	0.0	2.0369482034626082E-4	0.0	3.1980426285730195	0.0
80-81	0.0	2.0369482034626082E-4	0.0	3.5358025898438443	0.0
82-83	0.0	2.0369482034626082E-4	0.0	3.88427350375121	0.0
84-85	0.0	2.0369482034626082E-4	0.0	4.221099863762115	0.0
86-87	0.0	2.0369482034626082E-4	0.0	4.577396053684449	0.0
88	0.0	2.0369482034626082E-4	0.0	4.867253783037178	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	8515	0.0	28.261076	1
TACATGG	8575	0.0	26.802671	2
TCAACGC	4615	0.0	25.46078	4
GTATCAA	4720	0.0	25.292692	1
CAACGCA	4710	0.0	24.947237	5
ATCAACG	4800	0.0	24.871147	3
ACATGGG	8920	0.0	24.44874	3
GGTATCA	3370	0.0	23.7095	1
AACGCAG	4975	0.0	23.61839	6
TATCAAC	5535	0.0	21.738306	2
CATGGGG	5865	0.0	21.556965	4
GAGTACA	6315	0.0	20.24412	1
CGCAGAG	5830	0.0	19.993391	8
ACGCAGA	5960	0.0	19.636154	7
AGTACAT	6165	0.0	18.983208	2
TACACCG	485	0.0	18.412603	5
GTCTAGG	835	0.0	18.012203	1
TACCGCG	195	2.468183E-4	16.872007	8
GCAGAGT	6970	0.0	16.048985	9
GTATAGA	1215	0.0	15.860283	1
>>END_MODULE