FastQCFastQC Report
Wed 25 May 2016
SRR1032950_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032950_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2945583
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42980.1459133896413715No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36440.12371065422362908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47700.039.5922361
GTATCAA80100.037.5948071
TCAACGC88600.033.1915474
CAACGCA89350.033.0148935
ATCAACG89700.032.7320633
AACGCAG92200.032.197936
TATCAAC95200.031.1386382
GTACATG82500.028.4150851
ACGCAGA106800.027.443937
CGCAGAG108200.026.914918
TACATGG81700.026.4354442
ACATGGG83350.024.556413
GCAGAGT120700.023.9722849
TACACCG4200.023.523775
GAGTACT73900.021.57900212-13
GTATAGA11050.020.40711
ACACCGT5650.019.9844556
CATGGGG54500.019.7706174
AGAGTAC104200.019.7443310-11
CAGAGTA118750.019.55999210-11
Produced by FastQC (version 0.11.5)