##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032950_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2945583 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.86374106586031 34.0 31.0 34.0 31.0 34.0 2 32.905792503555325 34.0 31.0 34.0 31.0 34.0 3 32.991433953821705 34.0 33.0 34.0 31.0 34.0 4 36.394636307990645 37.0 37.0 37.0 35.0 37.0 5 36.37867342390284 37.0 37.0 37.0 35.0 37.0 6 36.433265333212475 37.0 37.0 37.0 35.0 37.0 7 36.39516082215303 37.0 37.0 37.0 35.0 37.0 8 36.32611744432257 37.0 37.0 37.0 35.0 37.0 9 38.1756966277983 39.0 39.0 39.0 37.0 39.0 10-11 38.18987989813901 39.0 39.0 39.0 37.0 39.0 12-13 38.19272755172745 39.0 39.0 39.0 37.0 39.0 14-15 39.76069474192376 41.0 40.0 41.0 37.0 41.0 16-17 39.7722607035687 41.0 40.0 41.0 37.0 41.0 18-19 39.79156655914975 41.0 40.0 41.0 37.5 41.0 20-21 39.803335876123676 41.0 40.0 41.0 37.5 41.0 22-23 39.773397150920545 41.0 40.0 41.0 37.0 41.0 24-25 39.76188143399796 41.0 40.0 41.0 37.0 41.0 26-27 39.693203009387275 41.0 40.0 41.0 37.0 41.0 28-29 39.63662320837675 41.0 40.0 41.0 37.0 41.0 30-31 39.53811826045981 41.0 40.0 41.0 37.0 41.0 32-33 39.428268869015064 41.0 40.0 41.0 36.0 41.0 34-35 39.351027623394074 41.0 39.0 41.0 36.0 41.0 36-37 39.27167083731811 41.0 39.0 41.0 35.5 41.0 38-39 39.17806950270965 41.0 39.0 41.0 35.0 41.0 40-41 39.0853179149934 41.0 39.0 41.0 35.0 41.0 42-43 38.967748999094574 41.0 39.0 41.0 35.0 41.0 44-45 38.81881023213401 41.0 39.0 41.0 35.0 41.0 46-47 38.62710030577988 41.0 39.0 41.0 35.0 41.0 48-49 38.45158445713463 41.0 39.0 41.0 35.0 41.0 50-51 38.31415308955816 41.0 38.5 41.0 35.0 41.0 52-53 38.18434517037883 40.5 38.0 41.0 34.0 41.0 54-55 38.04809421428627 40.0 38.0 41.0 34.0 41.0 56-57 37.85262696722516 40.0 37.5 41.0 34.0 41.0 58-59 37.69204330687677 40.0 37.0 41.0 34.0 41.0 60-61 37.513241012050926 40.0 36.5 41.0 33.0 41.0 62-63 37.2871737106033 39.5 36.0 41.0 33.0 41.0 64-65 37.00897479378446 39.0 35.5 41.0 33.0 41.0 66-67 36.74133405848689 39.0 35.0 41.0 32.5 41.0 68-69 36.449748997057625 38.5 35.0 41.0 32.0 41.0 70-71 36.12469025656381 37.0 35.0 40.0 32.0 41.0 72-73 35.782277226613544 37.0 35.0 39.5 32.0 41.0 74-75 35.432381976674904 36.5 35.0 39.0 31.0 41.0 76-77 35.0744158287171 36.0 35.0 39.0 31.0 40.5 78-79 34.71849851116061 35.5 35.0 37.5 31.0 39.5 80-81 34.373442031679296 35.0 35.0 37.0 31.0 39.0 82-83 34.05328605576553 35.0 34.5 37.0 31.0 39.0 84-85 33.766541631996105 35.0 34.0 36.0 30.5 38.0 86-87 33.60856764178772 35.0 34.0 36.0 31.0 37.0 88-89 33.41161410152082 35.0 34.0 36.0 31.0 37.0 90-91 33.23050224692362 35.0 34.0 35.0 30.0 36.5 92-93 33.06709232094292 35.0 34.0 35.0 30.0 36.0 94-95 32.966543804740866 35.0 34.0 35.0 30.0 36.0 96-97 32.90461701469624 35.0 34.0 35.0 30.0 36.0 98-99 32.8003714375049 35.0 34.0 35.0 30.0 36.0 100 32.7183379317439 35.0 34.0 35.0 30.0 36.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18296606334560295 1101 2 0.21060541248992593 1101 3 0.19983285315554866 1101 4 0.09646580648127667 1101 5 0.12131535903918689 1101 6 0.12249053386354092 1101 7 0.09506888607832309 1101 8 0.06628935361048605 1101 9 0.1809005360551197 1101 10-11 0.17513536482202596 1101 12-13 0.197127397377578 1101 14-15 0.2973664842254209 1101 16-17 0.2545080798306927 1101 18-19 0.2951456399735548 1101 20-21 0.2927779486046518 1101 22-23 0.284531063658207 1101 24-25 0.271656646328708 1101 26-27 0.28285069589833967 1101 28-29 0.26402519842176986 1101 30-31 0.3082516651258089 1101 32-33 0.3470999934955401 1101 34-35 0.35155487545445396 1101 36-37 0.40681669292460043 1101 38-39 0.43270596631683134 1101 40-41 0.45708375602368534 1101 42-43 0.48145717434933744 1101 44-45 0.4952467937895051 1101 46-47 0.5169554718008484 1101 48-49 0.5107478051921959 1101 50-51 0.5439702622046951 1101 52-53 0.5662105055263069 1101 54-55 0.5782971697221413 1101 56-57 0.5771785122639983 1101 58-59 0.6008643055670859 1101 60-61 0.6180608379355306 1101 62-63 0.5849524230291294 1101 64-65 0.625859746077353 1101 66-67 0.5932434151525996 1101 68-69 0.5287857377177687 1101 70-71 0.5180328214236525 1101 72-73 0.492487019424118 1101 74-75 0.5525747168740764 1101 76-77 0.5421404590411285 1101 78-79 0.5271762766807484 1101 80-81 0.5169482546610098 1101 82-83 0.5678144506686209 1101 84-85 0.5356589727289602 1101 86-87 0.5390472335290823 1101 88-89 0.5092395099105147 1101 90-91 0.4981738098895292 1101 92-93 0.5203599643201429 1101 94-95 0.5113158000877078 1101 96-97 0.5000387673495155 1101 98-99 0.556653554118391 1101 100 0.5263161179232085 1102 1 0.1792519455010506 1102 2 0.20365140879679444 1102 3 0.18881514289871149 1102 4 0.0999898118531064 1102 5 0.10994680104018784 1102 6 0.09197223606263805 1102 7 0.11924590471186747 1102 8 0.0574442973741256 1102 9 0.12800478315936203 1102 10-11 0.13988565859405355 1102 12-13 0.13889569517847633 1102 14-15 0.20805034355058893 1102 16-17 0.20146103433798856 1102 18-19 0.20752855083472355 1102 20-21 0.23452547252608724 1102 22-23 0.22803765254523256 1102 24-25 0.1715666261843367 1102 26-27 0.23234079158408605 1102 28-29 0.253847886016203 1102 30-31 0.2325138776488913 1102 32-33 0.24927852295667918 1102 34-35 0.21223055113013345 1102 36-37 0.22027963568102393 1102 38-39 0.25061409960540004 1102 40-41 0.26372741054778004 1102 42-43 0.25112731032382385 1102 44-45 0.2867616082934461 1102 46-47 0.24769535764943384 1102 48-49 0.2319377917626113 1102 50-51 0.2535923454680784 1102 52-53 0.24334895608867413 1102 54-55 0.22198212020057184 1102 56-57 0.21879195023613818 1102 58-59 0.17727986709135024 1102 60-61 0.22667927003222132 1102 62-63 0.19944000066888634 1102 64-65 0.27640301450105653 1102 66-67 0.3738590773986985 1102 68-69 0.29744089131857976 1102 70-71 0.3260788177305187 1102 72-73 0.33698865615401274 1102 74-75 0.25162310532464005 1102 76-77 0.26568397926942566 1102 78-79 0.23419470038395218 1102 80-81 0.23140036981964585 1102 82-83 0.23533648357108916 1102 84-85 0.2631807957942627 1102 86-87 0.284792994818325 1102 88-89 0.21819410114771642 1102 90-91 0.15960028294643536 1102 92-93 0.23142971419424185 1102 94-95 0.25094333911741984 1102 96-97 0.2637803738628577 1102 98-99 0.22697670335458753 1102 100 0.21934742562842757 1103 1 0.16923367106427634 1103 2 0.20685679653507094 1103 3 0.16136001004557698 1103 4 0.10567664910572461 1103 5 0.09228404261241963 1103 6 0.11892772041802147 1103 7 0.12201214466828958 1103 8 -0.09887759035863297 1103 9 0.039691221061247006 1103 10-11 0.11615304507354551 1103 12-13 0.1316741410968234 1103 14-15 0.16270229650449153 1103 16-17 0.21647383840584666 1103 18-19 0.22388214738374757 1103 20-21 0.1846535592128049 1103 22-23 0.20938629401660336 1103 24-25 0.18527647947355064 1103 26-27 0.23764852168924477 1103 28-29 0.2402362601250161 1103 30-31 0.2668304357976723 1103 32-33 0.2548429785330484 1103 34-35 0.3098253371186246 1103 36-37 0.26496263493385186 1103 38-39 0.2820729766016896 1103 40-41 0.30674560493036296 1103 42-43 0.2991671791453925 1103 44-45 0.23646170417443102 1103 46-47 0.3456234726771683 1103 48-49 0.34066091380199026 1103 50-51 0.4079238056563099 1103 52-53 0.4509698148951884 1103 54-55 0.37002679687370943 1103 56-57 0.43412446493113066 1103 58-59 0.40941927362302977 1103 60-61 0.36560777704938374 1103 62-63 0.40311186502950136 1103 64-65 0.39231513985669864 1103 66-67 0.44295020481918357 1103 68-69 0.42878852061067363 1103 70-71 0.42687715113133606 1103 72-73 0.38078024752067563 1103 74-75 0.38012950137751034 1103 76-77 0.4168206335936375 1103 78-79 0.42391010777691207 1103 80-81 0.3828367672343589 1103 82-83 0.41028373522830464 1103 84-85 0.32553172939162067 1103 86-87 0.3249624796374775 1103 88-89 0.33956658864269684 1103 90-91 0.32859488527039815 1103 92-93 0.35358065956967977 1103 94-95 0.34542716909337656 1103 96-97 0.34495335622149526 1103 98-99 0.3424350733410719 1103 100 0.2720035220762327 1104 1 0.1354612641900772 1104 2 0.15698131201656196 1104 3 0.16165176550057936 1104 4 0.07890272620829109 1104 5 0.0670840519839544 1104 6 0.08300908747799696 1104 7 0.11297955050034858 1104 8 0.011359998043822372 1104 9 0.03553271120665613 1104 10-11 0.09832139924345284 1104 12-13 0.1137437515170916 1104 14-15 0.20879545888386275 1104 16-17 0.14430351267635189 1104 18-19 0.14330735819363838 1104 20-21 0.19485394879474427 1104 22-23 0.18848314842865221 1104 24-25 0.14851683933180482 1104 26-27 0.17666759785443986 1104 28-29 0.16301859265242058 1104 30-31 0.2126263059992084 1104 32-33 0.2681766172518323 1104 34-35 0.22948074740305202 1104 36-37 0.21928660549572498 1104 38-39 0.2630195495470744 1104 40-41 0.2691793272636076 1104 42-43 0.23292529110742777 1104 44-45 0.21942105821901237 1104 46-47 0.2664632001333658 1104 48-49 0.18542891516881355 1104 50-51 0.11725873141448773 1104 52-53 0.18992341737882157 1104 54-55 0.18480040348170235 1104 56-57 0.22220940975288528 1104 58-59 0.21714425078543798 1104 60-61 0.17478802268537663 1104 62-63 0.16766935709961217 1104 64-65 0.21112215511661958 1104 66-67 0.23710871909431575 1104 68-69 0.19914796775177024 1104 70-71 0.20895312159949242 1104 72-73 0.2001201032829698 1104 74-75 0.17240138563370522 1104 76-77 0.18944367206314183 1104 78-79 0.1656285076220172 1104 80-81 0.14396428160828378 1104 82-83 0.09228192081881303 1104 84-85 0.09926465423784947 1104 86-87 0.09224517763278328 1104 88-89 0.08618775103622056 1104 90-91 0.06087651570597785 1104 92-93 0.10373957788191746 1104 94-95 0.08771032019375014 1104 96-97 0.06474975953981499 1104 98-99 0.11977308441319678 1104 100 0.07335187433204737 1105 1 0.07522341565116619 1105 2 0.12582670083861558 1105 3 0.11514670693561158 1105 4 0.1097697773568953 1105 5 0.051534968116037305 1105 6 0.1035604780153534 1105 7 0.09736550641976294 1105 8 0.010995310986736229 1105 9 0.09534921764959137 1105 10-11 0.10552383637288898 1105 12-13 0.131453952687977 1105 14-15 0.16173715407774836 1105 16-17 0.16517793215396637 1105 18-19 0.1753943705999319 1105 20-21 0.17897101810099514 1105 22-23 0.1680795846944534 1105 24-25 0.16787379247353584 1105 26-27 0.1309123060436903 1105 28-29 0.11995716594734773 1105 30-31 0.14342471515110589 1105 32-33 0.17383748717170278 1105 34-35 0.1962261703926913 1105 36-37 0.11712135676555135 1105 38-39 0.16088080331409316 1105 40-41 0.26329320727733574 1105 42-43 0.15988752991566457 1105 44-45 0.16676583333104134 1105 46-47 0.1274695840612381 1105 48-49 0.13665413725153996 1105 50-51 0.24329416043939744 1105 52-53 0.1207175152462483 1105 54-55 0.1490548826814475 1105 56-57 0.16948396118225162 1105 58-59 0.16980610923258155 1105 60-61 0.19270675248680647 1105 62-63 0.13603553210527508 1105 64-65 0.13461735417073584 1105 66-67 0.20703280187119333 1105 68-69 0.212996104422551 1105 70-71 0.16586191264708106 1105 72-73 0.2361626266190484 1105 74-75 0.1670058263413523 1105 76-77 0.18666358126802862 1105 78-79 0.1804663882495845 1105 80-81 0.17611720641276918 1105 82-83 0.17800747481754797 1105 84-85 0.2214253229556249 1105 86-87 0.16748103266549919 1105 88-89 0.16296178244003556 1105 90-91 0.13725311545039887 1105 92-93 0.09836464927598598 1105 94-95 0.11570290838160702 1105 96-97 0.1583526650598941 1105 98-99 0.11028561361421652 1105 100 0.07879059936831823 1106 1 0.09376121302496898 1106 2 0.13831240699876446 1106 3 0.08878600102246281 1106 4 0.054141945094180244 1106 5 0.06435948559111893 1106 6 0.04431926639934147 1106 7 0.0692534393713018 1106 8 0.03288945319902581 1106 9 0.04953414539492229 1106 10-11 0.0750764944760931 1106 12-13 0.07696993161630417 1106 14-15 0.10423423146914956 1106 16-17 0.12294819497821408 1106 18-19 0.15322487769361715 1106 20-21 0.10138664701551647 1106 22-23 0.11728783174794444 1106 24-25 0.15068964434383503 1106 26-27 0.1578749378460813 1106 28-29 0.17174209661110496 1106 30-31 0.19973328607416363 1106 32-33 0.1879672414172191 1106 34-35 0.16037312360648315 1106 36-37 0.1579936446600243 1106 38-39 0.11255451448968756 1106 40-41 0.14812738049487706 1106 42-43 0.16946392761114026 1106 44-45 0.17811624038341733 1106 46-47 0.25832667636702666 1106 48-49 0.19239832665269319 1106 50-51 0.1984062972565468 1106 52-53 0.17351340780125923 1106 54-55 0.1884240278691678 1106 56-57 0.16577664490986876 1106 58-59 0.10719601265539325 1106 60-61 0.11075826670282396 1106 62-63 0.16810176584130687 1106 64-65 0.1982707129551997 1106 66-67 0.13049306100636215 1106 68-69 0.15973713933684053 1106 70-71 0.17665698226604576 1106 72-73 0.18576501352880825 1106 74-75 0.19945236779831532 1106 76-77 0.18485886761070702 1106 78-79 0.15264206001636182 1106 80-81 0.16271683130349857 1106 82-83 0.20528490169989055 1106 84-85 0.23479886980241105 1106 86-87 0.20484999028338535 1106 88-89 0.16140779523582438 1106 90-91 0.18466427932122542 1106 92-93 0.15858847450589764 1106 94-95 0.18564943273839418 1106 96-97 0.1822260186367899 1106 98-99 0.2033336279959812 1106 100 0.2720740184532886 1107 1 0.081737129236231 1107 2 0.06413720678258983 1107 3 0.043108653442217815 1107 4 0.026968238831535984 1107 5 0.08286239895578973 1107 6 0.038488557578958194 1107 7 0.05268746315342554 1107 8 0.021923230603057675 1107 9 0.06908734924193283 1107 10-11 0.07001676060015427 1107 12-13 0.038798579456369 1107 14-15 0.09667016967041064 1107 16-17 0.09098198635894761 1107 18-19 0.13563886957004456 1107 20-21 0.1429380059386247 1107 22-23 0.09255320456077953 1107 24-25 0.1350360115287188 1107 26-27 0.11496070545400272 1107 28-29 0.09802060518891409 1107 30-31 0.11774562225564011 1107 32-33 0.10847085089900332 1107 34-35 0.17632135897094514 1107 36-37 0.1290663725927601 1107 38-39 0.13263495037625006 1107 40-41 0.16197527754564334 1107 42-43 0.15729546649198056 1107 44-45 0.1500397996966356 1107 46-47 0.12690440873348052 1107 48-49 0.16873747856229926 1107 50-51 0.183024821775561 1107 52-53 0.1928871712518898 1107 54-55 0.18214400223745741 1107 56-57 0.23640567097485388 1107 58-59 0.19181474100122387 1107 60-61 0.2015641824171368 1107 62-63 0.1664261395300244 1107 64-65 0.13038475910780534 1107 66-67 0.11943613547575183 1107 68-69 0.14388962839230857 1107 70-71 0.1868924129191143 1107 72-73 0.20881066860102493 1107 74-75 0.18742505675159293 1107 76-77 0.200759883095337 1107 78-79 0.18951260882685972 1107 80-81 0.20464924931852835 1107 82-83 0.19674880435840691 1107 84-85 0.19870473131820177 1107 86-87 0.16460110900143832 1107 88-89 0.16389957810318379 1107 90-91 0.18864724949340683 1107 92-93 0.18072872599325507 1107 94-95 0.1441831229821986 1107 96-97 0.1571285797110633 1107 98-99 0.12520052345340815 1107 100 0.14770369773470549 1108 1 0.052163478296300525 1108 2 0.06325893991595422 1108 3 0.055289382919461616 1108 4 -0.013991071023120583 1108 5 -0.007427425345660765 1108 6 0.020434959583496948 1108 7 0.024415401451129526 1108 8 -0.022142764771075463 1108 9 0.03212485007012589 1108 10-11 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2214 50-51 -0.15372071457385061 2214 52-53 -0.1674436901793186 2214 54-55 -0.130073343210519 2214 56-57 -0.12025906002966735 2214 58-59 -0.10917610598433924 2214 60-61 -0.14684679627553265 2214 62-63 -0.09358202034609775 2214 64-65 -0.11456422285702672 2214 66-67 -0.162069330888869 2214 68-69 -0.1560082192608192 2214 70-71 -0.16784417971360455 2214 72-73 -0.14473785154080332 2214 74-75 -0.09571939562701459 2214 76-77 -0.14498631124241257 2214 78-79 -0.09372760068976049 2214 80-81 -0.12716222949999434 2214 82-83 -0.148954812251894 2214 84-85 -0.15374719859852348 2214 86-87 -0.10202113319298434 2214 88-89 -0.180641102264957 2214 90-91 -0.20492621538287636 2214 92-93 -0.12810172368227057 2214 94-95 -0.127902747915293 2214 96-97 -0.13222384212375005 2214 98-99 -0.12631534624028262 2214 100 -0.15639463937419862 2215 1 -0.17982469036996207 2215 2 -0.21930705264372108 2215 3 -0.19262914779631046 2215 4 -0.11381884750598914 2215 5 -0.15051793312726147 2215 6 -0.17831661833145773 2215 7 -0.1714583352770518 2215 8 -0.11121135425921835 2215 9 -0.183156551377337 2215 10-11 -0.21288964858231907 2215 12-13 -0.22489210970412898 2215 14-15 -0.2747295932727596 2215 16-17 -0.28934827382703787 2215 18-19 -0.28710508446330607 2215 20-21 -0.264006334880591 2215 22-23 -0.27846452043972647 2215 24-25 -0.29023731177745304 2215 26-27 -0.2762609426844733 2215 28-29 -0.28237587151575383 2215 30-31 -0.38597006978753257 2215 32-33 -0.3560307786547625 2215 34-35 -0.3253718253907678 2215 36-37 -0.3130160690926118 2215 38-39 -0.31341834162757465 2215 40-41 -0.3263330557311619 2215 42-43 -0.3154655503767003 2215 44-45 -0.333457895018924 2215 46-47 -0.35717541481349 2215 48-49 -0.34312210341731486 2215 50-51 -0.3673110723254922 2215 52-53 -0.4053749418774615 2215 54-55 -0.36817824159533785 2215 56-57 -0.3575897583048828 2215 58-59 -0.38405499728668246 2215 60-61 -0.34936378147154556 2215 62-63 -0.3106628814150838 2215 64-65 -0.3398682753214004 2215 66-67 -0.34861596276547857 2215 68-69 -0.3367468513985088 2215 70-71 -0.3673209628176579 2215 72-73 -0.3751177302309898 2215 74-75 -0.40792073167433784 2215 76-77 -0.40479738698666523 2215 78-79 -0.3975002419256839 2215 80-81 -0.4031746435684127 2215 82-83 -0.485127094354894 2215 84-85 -0.421809926187251 2215 86-87 -0.48309396689744233 2215 88-89 -0.4890914839385516 2215 90-91 -0.4849159961842915 2215 92-93 -0.49376104224045747 2215 94-95 -0.5534916894749884 2215 96-97 -0.5500674432281656 2215 98-99 -0.5575121536609444 2215 100 -0.5541123390413247 2216 1 -0.3437447993126739 2216 2 -0.3391675454943055 2216 3 -0.2999654640848135 2216 4 -0.2008690763731522 2216 5 -0.21266982081382935 2216 6 -0.21390079709819787 2216 7 -0.213497777335526 2216 8 -0.24598069902169328 2216 9 -0.3290711898317298 2216 10-11 -0.36230427545928023 2216 12-13 -0.403326230415459 2216 14-15 -0.5239029740293901 2216 16-17 -0.4994349740016517 2216 18-19 -0.4305237062493603 2216 20-21 -0.3669834386645192 2216 22-23 -0.454243251635539 2216 24-25 -0.42912674050703714 2216 26-27 -0.4231246342725825 2216 28-29 -0.4161491607215595 2216 30-31 -0.4791730840380666 2216 32-33 -0.5614301201458645 2216 34-35 -0.5560610629837655 2216 36-37 -0.5482788700293355 2216 38-39 -0.5679665038724195 2216 40-41 -0.47450776858594423 2216 42-43 -0.5537398509756954 2216 44-45 -0.5268996815780582 2216 46-47 -0.5508303647821222 2216 48-49 -0.6161933565177407 2216 50-51 -0.575360816127656 2216 52-53 -0.4781989127569375 2216 54-55 -0.5537937586091246 2216 56-57 -0.5875852005541446 2216 58-59 -0.5365300657172583 2216 60-61 -0.5785444688785191 2216 62-63 -0.5697452239208545 2216 64-65 -0.6979152979306491 2216 66-67 -0.652324265926552 2216 68-69 -0.6660734936978017 2216 70-71 -0.6455046744100059 2216 72-73 -0.5608093416937194 2216 74-75 -0.6029606599852428 2216 76-77 -0.5790229998042591 2216 78-79 -0.5772833024899384 2216 80-81 -0.6007244221028927 2216 82-83 -0.6248625666975869 2216 84-85 -0.6130973591642643 2216 86-87 -0.5625449627649886 2216 88-89 -0.6091795476781954 2216 90-91 -0.54598482930313 2216 92-93 -0.5778131300287086 2216 94-95 -0.6837018981637613 2216 96-97 -0.5987277158501385 2216 98-99 -0.6023860892044937 2216 100 -0.622865504283439 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19.0 3 3.0 4 9.0 5 16.0 6 49.0 7 137.0 8 277.0 9 553.0 10 987.0 11 1569.0 12 2562.0 13 3939.0 14 4923.0 15 6560.0 16 8079.0 17 11566.0 18 13674.0 19 7430.0 20 5224.0 21 5045.0 22 5169.0 23 5444.0 24 6134.0 25 7596.0 26 9292.0 27 12242.0 28 15593.0 29 20365.0 30 26588.0 31 33803.0 32 43191.0 33 56519.0 34 79117.0 35 121377.0 36 227693.0 37 540177.0 38 1066361.0 39 593902.0 40 2399.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.087440032890036 19.778121268765027 13.338321610090803 23.79611708825414 2 15.544344003696395 20.072291537164418 40.48404164486842 23.89932281427077 3 18.34603655172718 25.208694114128328 30.171080117016043 26.274189217128445 4 12.127355585328704 16.569719078348275 39.05597993232456 32.24694540399846 5 12.425574676548171 38.034981344867035 36.05054369782419 13.4889002807606 6 30.936134022981093 38.314833141795944 17.49628666441462 13.252746170808344 7 27.269301475268755 32.642022837723836 22.39848721722318 17.690188469784236 8 25.19264421244598 36.4992998001226 20.138047661189614 18.170008326241806 9 25.141170723128848 16.238477946541 21.246885553005946 37.37346577732421 10-11 23.904741438282336 26.69116775864065 28.971039009934536 20.43305179314248 12-13 24.752344996969185 24.00846760374317 29.336790943853323 21.90239645543432 14-15 21.74797641549068 26.017292025078277 26.617853375102168 25.61687818432888 16-17 21.023868619556808 29.608162458840916 27.11840066974857 22.249568251853706 18-19 20.880980995253907 28.21204991886148 29.793487190977668 21.113481894906947 20-21 21.95923523458684 27.50075282210686 29.507944607230556 21.032067336075745 22-23 21.564701658673044 27.288078483760824 29.63982444868621 21.507395408879923 24-25 21.440990802970088 27.371811335019817 29.578701307607474 21.60849655440262 26-27 21.260324787859847 27.346528034652007 29.669897071687558 21.723250105800588 28-29 21.24565884543028 27.468384986947253 29.703852538079424 21.582103629543038 30-31 21.477436053624196 27.798919788140758 29.345472559583293 21.378171598651754 32-33 21.21838078588771 27.582706592673944 29.69339041640684 21.505522205031504 34-35 21.350358394944845 27.339822520812984 29.738800242869118 21.571018841373053 36-37 21.62091346108944 27.742895631883634 29.200575933238206 21.435614973788724 38-39 21.466048339684047 27.880737930277906 29.238111476149246 21.415102253888797 40-41 21.308925564025728 27.57929377300924 29.423395832639816 21.68838483032522 42-43 21.3655409974691 27.559544264757367 29.265009625709144 21.80990511206439 44-45 21.483253962535535 27.71428076190528 29.17373028248572 21.628734993073465 46-47 21.576527674581914 27.923096061773855 28.8623616032978 21.638014660346435 48-49 21.57246014391712 27.843933383398262 28.675680732599762 21.907925740084856 50-51 21.642879454217987 27.814808810088753 28.601008725999932 21.94130300969333 52-53 21.64827344078453 27.88979154721634 28.522171623055048 21.93976338894408 54-55 21.6948199107265 28.200585809541373 28.42379504993487 21.68079922979726 56-57 21.574612817188136 27.857061418942784 28.526784417160883 22.041541346708197 58-59 21.41064756425376 27.512972744196908 28.8865264410191 22.189853250530234 60-61 21.732249108103606 27.855763314984827 28.514280791770545 21.897706785141025 62-63 21.481875662419366 27.819353672390108 28.593222602470902 22.105548062719624 64-65 21.500327778915075 27.62358079877566 28.713195316512895 22.162896105796374 66-67 21.403386018998617 27.72403629434309 28.50946654702991 22.363111139628387 68-69 21.796147281564853 27.749384035246 28.523950695660368 21.930517987528784 70-71 21.72664969888813 27.647056626820564 28.42719081417838 22.19910286011292 72-73 21.537349312513008 27.749226553792578 28.518361220851695 22.19506291284272 74-75 21.581245546297627 27.82914282164176 28.373534203585503 22.21607742847511 76-77 21.634379617613835 27.776950738945523 28.23716531450061 22.35150432894003 78-79 21.67269490375756 27.85707544383419 28.29578331209248 22.174446340315768 80-81 21.64535760971911 27.693100014139837 28.345026282613077 22.316516093527976 82-83 21.477454606130983 27.692173984779565 28.52959833540395 22.3007730736855 84-85 21.44986238717429 27.653829479597082 28.35830801576462 22.538000117464012 86-87 21.57263215108442 27.743719909144566 28.26957943629366 22.414068503477356 88-89 21.269540791609362 27.62240504296453 28.334831063200355 22.77322310222575 90-91 21.279939380297986 27.5327889917663 28.383199624385984 22.80407200354973 92-93 21.44576803933927 27.708882076742402 28.214168770542603 22.631181113375728 94-95 21.166174710457643 27.37106964786075 28.471779582343355 22.99097605933825 96-97 21.14538529214909 27.393398202355296 28.452851250001487 23.00836525549413 98-99 21.118818134891193 27.469991918403885 28.318126658522264 23.093063288182655 100 21.20222040933832 27.356418067323172 28.21614600573129 23.22521551760721 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 37.0 1 30.5 2 78.5 3 144.0 4 107.5 5 81.5 6 206.0 7 482.5 8 841.0 9 1046.5 10 899.0 11 780.5 12 880.5 13 1036.5 14 1232.5 15 1399.5 16 1618.5 17 1891.0 18 2154.5 19 2532.0 20 3111.0 21 3920.0 22 5147.0 23 7117.5 24 9872.5 25 13316.0 26 17997.5 27 24065.0 28 31835.5 29 40524.0 30 48924.0 31 57660.5 32 66379.0 33 74712.0 34 82942.0 35 89478.0 36 94395.5 37 98806.0 38 102431.0 39 104379.5 40 105311.5 41 106634.0 42 106804.0 43 105492.5 44 103974.0 45 102660.0 46 101493.5 47 99795.5 48 98563.0 49 95697.5 50 91161.5 51 87406.0 52 81822.5 53 77296.0 54 74156.5 55 70012.0 56 64763.5 57 58626.0 58 52605.5 59 47563.0 60 42405.5 61 36374.0 62 31184.5 63 25755.5 64 20591.5 65 16376.5 66 12485.5 67 9179.5 68 6882.0 69 5288.0 70 3800.5 71 2689.5 72 1986.5 73 1422.5 74 1012.0 75 681.0 76 455.0 77 302.0 78 196.0 79 106.0 80 46.0 81 18.5 82 12.0 83 7.5 84 5.0 85 4.0 86 1.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.3949136724376804E-4 2 3.3949136724376804E-4 3 1.3579654689750722E-4 4 0.011848248716807504 5 4.413387774168985E-4 6 0.004005998133476463 7 6.110844610387825E-4 8 0.1453023051803327 9 0.001222168922077565 10-11 0.0 12-13 1.1882197853531882E-4 14-15 1.8672025198407242E-4 16-17 0.0 18-19 1.0184741017313042E-4 20-21 0.0 22-23 1.8672025198407242E-4 24-25 4.2436420905471006E-4 26-27 0.006229666588923144 28-29 0.007672504899709158 30-31 0.014258637424238257 32-33 0.017602627391589375 34-35 0.026717970602084546 36-37 0.03885478698104925 38-39 0.04393018292134358 40-41 0.05625371955229237 42-43 0.07344895730318922 44-45 0.11118342277233403 46-47 0.11872013112514568 48-49 0.11554588684141646 50-51 0.11732821651944624 52-53 0.11155686327630218 54-55 0.118838953103681 56-57 0.10009902963182502 58-59 0.09432767638868095 60-61 0.0962967263186948 62-63 0.0941070069999725 64-65 0.0 66-67 0.0 68-69 1.6974568362188402E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 1.6974568362188402E-5 78-79 3.3949136724376805E-5 80-81 1.5277111525969562E-4 82-83 1.1882197853531882E-4 84-85 0.0 86-87 8.317538497472316E-4 88-89 4.752879141412753E-4 90-91 2.0369482034626084E-4 92-93 9.336012599203621E-4 94-95 0.0010014995333691157 96-97 2.2066938870844924E-4 98-99 5.092370508656521E-5 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2945583.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.83385812098191 #Duplication Level Percentage of deduplicated Percentage of total 1 75.44930226095391 47.407707535918576 2 14.809638521824878 18.610934514067452 3 4.676902924939904 8.816035644938376 4 1.93316889966648 4.858738414621532 5 0.9830682014634256 3.0884983947000872 6 0.5659552272827937 2.1336690272349075 7 0.3557548292946088 1.5647413928826113 8 0.24268522483559524 1.2199079188302708 9 0.1729122847506572 0.9778271370657856 >10 0.765645669559304 8.33115282174169 >50 0.03472079276932846 1.4867548225267055 >100 0.009758554563141163 1.021449634937983 >500 2.7033783152423797E-4 0.10990180703311088 >1k 2.162702644777475E-4 0.3726809335009568 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4298 0.1459133896413715 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3644 0.12371065422362908 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.6974568362188402E-5 0.0 18-19 0.0 0.0 0.0 3.3949136724376805E-5 0.0 20-21 0.0 0.0 0.0 5.092370508656521E-5 0.0 22-23 0.0 0.0 0.0 1.1882197853531882E-4 0.0 24-25 0.0 0.0 0.0 2.0369482034626082E-4 0.0 26-27 0.0 0.0 0.0 3.0554223051939124E-4 0.0 28-29 0.0 0.0 0.0 0.0011712452169909998 0.0 30-31 0.0 0.0 0.0 0.005177243350467462 0.0 32-33 0.0 0.0 0.0 0.013189239617420388 0.0 34-35 0.0 0.0 0.0 0.025529750816731357 0.0 36-37 0.0 0.0 0.0 0.047681562529387225 0.0 38-39 0.0 0.0 0.0 0.0875378490438056 0.0 40-41 0.0 0.0 0.0 0.13778257139588326 0.0 42-43 0.0 0.0 0.0 0.1889439204395191 0.0 44-45 0.0 0.0 0.0 0.248219113160281 0.0 46-47 0.0 0.0 0.0 0.32231310406123337 0.0 48-49 0.0 1.6974568362188402E-5 0.0 0.40946053803270865 0.0 50-51 0.0 3.3949136724376805E-5 0.0 0.5084562207209914 0.0 52-53 0.0 3.3949136724376805E-5 0.0 0.6056186500261578 0.0 54-55 0.0 3.3949136724376805E-5 0.0 0.7104366096626712 0.0 56-57 0.0 3.3949136724376805E-5 0.0 0.8354203565134644 0.0 58-59 0.0 3.3949136724376805E-5 0.0 0.9824031439616537 0.0 60-61 0.0 3.3949136724376805E-5 0.0 1.1448497631877967 0.0 62-63 0.0 6.789827344875361E-5 0.0 1.3126433714480292 0.0 64-65 0.0 6.789827344875361E-5 0.0 1.4833905546032824 0.0 66-67 0.0 6.789827344875361E-5 0.0 1.6745581434982482 0.0 68-69 0.0 8.487284181094201E-5 0.0 1.8970268364530893 0.0 70-71 0.0 1.0184741017313041E-4 0.0 2.143293874251719 0.0 72-73 0.0 1.0184741017313041E-4 0.0 2.397912399684545 0.0 74-75 0.0 1.0184741017313041E-4 0.0 2.6470311649680216 0.0 76-77 0.0 1.0184741017313041E-4 0.0 2.9125982869944593 0.0 78-79 0.0 1.0184741017313041E-4 0.0 3.2183272377658345 0.0 80-81 0.0 1.3579654689750722E-4 0.0 3.55849758774409 0.0 82-83 0.0 1.3579654689750722E-4 0.0 3.9092770429487134 0.0 84-85 0.0 1.3579654689750722E-4 0.0 4.248921181307741 0.0 86-87 0.0 1.3579654689750722E-4 0.0 4.607882378462939 0.0 88 0.0 1.3579654689750722E-4 0.0 4.90038814048017 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4770 0.0 39.592236 1 GTATCAA 8010 0.0 37.594807 1 TCAACGC 8860 0.0 33.191547 4 CAACGCA 8935 0.0 33.014893 5 ATCAACG 8970 0.0 32.732063 3 AACGCAG 9220 0.0 32.19793 6 TATCAAC 9520 0.0 31.138638 2 GTACATG 8250 0.0 28.415085 1 ACGCAGA 10680 0.0 27.44393 7 CGCAGAG 10820 0.0 26.91491 8 TACATGG 8170 0.0 26.435444 2 ACATGGG 8335 0.0 24.55641 3 GCAGAGT 12070 0.0 23.972284 9 TACACCG 420 0.0 23.52377 5 GAGTACT 7390 0.0 21.579002 12-13 GTATAGA 1105 0.0 20.4071 1 ACACCGT 565 0.0 19.984455 6 CATGGGG 5450 0.0 19.770617 4 AGAGTAC 10420 0.0 19.74433 10-11 CAGAGTA 11875 0.0 19.559992 10-11 >>END_MODULE