##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032949_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1525 Sequences flagged as poor quality 0 Sequence length 100 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 16.79016393442623 20.0 2.0 30.0 2.0 31.0 2 16.60655737704918 16.0 2.0 30.0 2.0 31.0 3 16.72 16.0 2.0 30.0 2.0 31.0 4 18.58032786885246 19.0 2.0 33.0 2.0 35.0 5 18.630819672131146 22.0 2.0 35.0 2.0 35.0 6 18.53704918032787 19.0 2.0 35.0 2.0 36.0 7 18.448524590163935 19.0 2.0 35.0 2.0 36.0 8 18.297049180327868 19.0 2.0 35.0 2.0 37.0 9 19.007213114754098 22.0 2.0 35.0 2.0 39.0 10-11 18.994754098360655 22.5 2.0 35.0 2.0 39.0 12-13 18.67377049180328 19.0 2.0 35.0 2.0 39.0 14-15 18.762622950819672 18.5 2.0 35.0 2.0 39.0 16-17 18.640983606557377 17.5 2.0 35.0 2.0 39.0 18-19 18.440327868852457 17.5 2.0 35.5 2.0 39.0 20-21 18.281967213114754 14.5 2.0 35.5 2.0 40.0 22-23 17.92950819672131 10.0 2.0 36.0 2.0 40.0 24-25 17.788196721311476 10.0 2.0 35.0 2.0 39.5 26-27 17.210819672131148 8.0 2.0 34.5 2.0 39.0 28-29 16.875081967213113 2.0 2.0 34.0 2.0 39.0 30-31 16.75704918032787 2.0 2.0 34.0 2.0 39.0 32-33 16.456065573770495 2.0 2.0 34.0 2.0 39.0 34-35 16.155409836065573 2.0 2.0 34.0 2.0 39.0 36-37 15.99344262295082 2.0 2.0 33.5 2.0 39.0 38-39 16.02360655737705 2.0 2.0 34.0 2.0 39.0 40-41 15.86655737704918 2.0 2.0 33.5 2.0 39.0 42-43 15.64327868852459 2.0 2.0 33.0 2.0 39.0 44-45 15.554426229508197 2.0 2.0 33.0 2.0 39.0 46-47 15.432131147540984 2.0 2.0 33.0 2.0 39.0 48-49 15.078688524590163 2.0 2.0 33.0 2.0 38.5 50-51 15.01704918032787 2.0 2.0 33.0 2.0 38.0 52-53 14.718032786885246 2.0 2.0 32.0 2.0 38.5 54-55 14.575409836065575 2.0 2.0 32.0 2.0 38.0 56-57 14.267868852459017 2.0 2.0 31.5 2.0 38.0 58-59 14.211803278688524 2.0 2.0 31.5 2.0 38.0 60-61 14.059344262295081 2.0 2.0 31.5 2.0 38.0 62-63 13.740655737704918 2.0 2.0 31.0 2.0 38.0 64-65 13.487868852459016 2.0 2.0 30.5 2.0 37.0 66-67 13.393770491803277 2.0 2.0 30.0 2.0 37.0 68-69 13.145573770491804 2.0 2.0 30.0 2.0 36.5 70-71 13.014426229508196 2.0 2.0 30.0 2.0 36.0 72-73 12.832459016393443 2.0 2.0 29.5 2.0 36.0 74-75 12.562295081967214 2.0 2.0 29.5 2.0 35.0 76-77 12.476393442622951 2.0 2.0 29.0 2.0 35.0 78-79 12.101639344262296 2.0 2.0 28.5 2.0 35.0 80-81 11.951803278688525 2.0 2.0 29.0 2.0 35.0 82-83 11.83672131147541 2.0 2.0 29.0 2.0 34.5 84-85 11.657377049180328 2.0 2.0 29.0 2.0 34.0 86-87 11.463934426229507 2.0 2.0 28.0 2.0 34.0 88-89 11.261311475409837 2.0 2.0 27.5 2.0 34.0 90-91 11.289180327868852 2.0 2.0 28.5 2.0 34.0 92-93 10.999016393442623 2.0 2.0 28.0 2.0 34.0 94-95 10.720655737704918 2.0 2.0 26.5 2.0 34.0 96-97 10.321639344262294 2.0 2.0 26.0 2.0 34.0 98-99 10.040655737704917 2.0 2.0 25.0 2.0 34.0 100 9.973770491803279 2.0 2.0 25.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1102 1 -14.789682539682545 1102 2 -14.11111111111111 1102 3 -14.23941798941799 1102 4 -16.694444444444443 1102 5 -16.288359788359784 1102 6 -16.089947089947092 1102 7 -16.77777777777778 1102 8 -15.670634920634924 1102 9 -15.841269841269842 1102 10-11 -16.670634920634924 1102 12-13 -17.131613756613756 1102 14-15 -17.060185185185187 1102 16-17 -16.521164021164015 1102 18-19 -16.452380952380953 1102 20-21 -16.41137566137566 1102 22-23 -16.254629629629626 1102 24-25 -15.798280423280428 1102 26-27 -14.581349206349202 1102 28-29 -14.444444444444443 1102 30-31 -14.764550264550266 1102 32-33 -14.55357142857143 1102 34-35 -13.991402116402117 1102 36-37 -13.660052910052908 1102 38-39 -14.01058201058201 1102 40-41 -13.178571428571429 1102 42-43 -13.293650793650794 1102 44-45 -13.146164021164017 1102 46-47 -13.149470899470899 1102 48-49 -12.892195767195767 1102 50-51 -12.814814814814813 1102 52-53 -11.780423280423278 1102 54-55 -11.625661375661377 1102 56-57 -11.681878306878307 1102 58-59 -12.11441798941799 1102 60-61 -11.756613756613756 1102 62-63 -11.66335978835979 1102 64-65 -11.228835978835976 1102 66-67 -11.178571428571429 1102 68-69 -10.867724867724867 1102 70-71 -10.388888888888888 1102 72-73 -10.356481481481481 1102 74-75 -9.632936507936511 1102 76-77 -9.838624338624337 1102 78-79 -9.703703703703704 1102 80-81 -9.26719576719577 1102 82-83 -9.451058201058203 1102 84-85 -9.412698412698413 1102 86-87 -9.267195767195766 1102 88-89 -8.982804232804233 1102 90-91 -8.72089947089947 1102 92-93 -8.713624338624339 1102 94-95 -8.351190476190476 1102 96-97 -7.777116402116402 1102 98-99 -7.882275132275133 1102 100 -7.777777777777777 1103 1 -14.789682539682545 1103 2 -14.11111111111111 1103 3 -14.23941798941799 1103 4 -16.694444444444443 1103 5 -16.288359788359784 1103 6 -16.089947089947092 1103 7 -16.77777777777778 1103 8 -15.670634920634924 1103 9 -15.841269841269842 1103 10-11 -16.670634920634924 1103 12-13 -17.131613756613756 1103 14-15 -17.060185185185187 1103 16-17 -16.521164021164015 1103 18-19 -16.452380952380953 1103 20-21 -16.41137566137566 1103 22-23 -16.254629629629626 1103 24-25 -15.798280423280428 1103 26-27 -14.581349206349202 1103 28-29 -14.444444444444443 1103 30-31 -14.764550264550266 1103 32-33 -14.55357142857143 1103 34-35 -13.991402116402117 1103 36-37 -13.660052910052908 1103 38-39 -14.01058201058201 1103 40-41 -13.178571428571429 1103 42-43 -13.293650793650794 1103 44-45 -13.146164021164017 1103 46-47 -13.149470899470899 1103 48-49 -12.892195767195767 1103 50-51 -12.814814814814813 1103 52-53 -11.780423280423278 1103 54-55 -11.625661375661377 1103 56-57 -11.681878306878307 1103 58-59 -12.11441798941799 1103 60-61 -11.756613756613756 1103 62-63 -11.66335978835979 1103 64-65 -11.228835978835976 1103 66-67 -11.178571428571429 1103 68-69 -10.867724867724867 1103 70-71 -10.388888888888888 1103 72-73 -10.356481481481481 1103 74-75 -9.632936507936511 1103 76-77 -9.838624338624337 1103 78-79 -9.703703703703704 1103 80-81 -9.26719576719577 1103 82-83 -9.451058201058203 1103 84-85 -9.412698412698413 1103 86-87 -9.267195767195766 1103 88-89 -8.982804232804233 1103 90-91 -8.72089947089947 1103 92-93 -8.713624338624339 1103 94-95 -8.351190476190476 1103 96-97 -7.777116402116402 1103 98-99 -7.882275132275133 1103 100 -7.777777777777777 1104 1 -5.123015873015879 1104 2 -5.777777777777777 1104 3 -4.572751322751325 1104 4 -5.694444444444443 1104 5 -5.288359788359784 1104 6 -4.423280423280426 1104 7 -7.444444444444445 1104 8 -6.6706349206349245 1104 9 -10.841269841269842 1104 10-11 -6.6706349206349245 1104 12-13 -5.96494708994709 1104 14-15 -6.226851851851853 1104 16-17 -5.687830687830683 1104 18-19 -4.785714285714285 1104 20-21 -5.078042328042329 1104 22-23 -3.754629629629626 1104 24-25 -3.7982804232804277 1104 26-27 -1.914682539682536 1104 28-29 -1.7777777777777768 1104 30-31 -2.431216931216934 1104 32-33 -3.2202380952380985 1104 34-35 -2.991402116402117 1104 36-37 -4.49338624338624 1104 38-39 -7.8439153439153415 1104 40-41 -4.011904761904761 1104 42-43 -6.9603174603174605 1104 44-45 -1.146164021164017 1104 46-47 -1.1494708994708986 1104 48-49 -0.22552910052910136 1104 50-51 -1.314814814814813 1104 52-53 -1.2804232804232782 1104 54-55 -2.7923280423280445 1104 56-57 -2.515211640211641 1104 58-59 -3.9477513227513246 1104 60-61 -1.0899470899470884 1104 62-63 -1.4966931216931219 1104 64-65 -2.228835978835976 1104 66-67 -0.5119047619047628 1104 68-69 -5.2010582010582 1104 70-71 -1.3888888888888875 1104 72-73 0.3101851851851851 1104 74-75 1.0337301587301546 1104 76-77 0.6613756613756632 1104 78-79 -2.5370370370370363 1104 80-81 -1.9338624338624353 1104 82-83 -0.6177248677248706 1104 84-85 -0.24603174603174693 1104 86-87 -4.6005291005291 1104 88-89 0.8505291005290996 1104 90-91 1.9457671957671963 1104 92-93 1.9530423280423275 1104 94-95 2.31547619047619 1104 96-97 2.0562169312169303 1104 98-99 1.4510582010581992 1104 100 2.222222222222223 1105 1 -2.789682539682545 1105 2 -4.111111111111111 1105 3 -5.239417989417991 1105 4 -2.694444444444443 1105 5 -2.2883597883597844 1105 6 -3.089947089947092 1105 7 -2.2777777777777786 1105 8 -15.670634920634924 1105 9 -15.841269841269842 1105 10-11 -16.670634920634924 1105 12-13 -17.131613756613756 1105 14-15 -17.060185185185187 1105 16-17 -16.521164021164015 1105 18-19 -16.452380952380953 1105 20-21 -16.41137566137566 1105 22-23 -16.254629629629626 1105 24-25 -15.798280423280428 1105 26-27 -14.581349206349202 1105 28-29 -14.444444444444443 1105 30-31 -14.764550264550266 1105 32-33 -14.55357142857143 1105 34-35 -13.991402116402117 1105 36-37 -13.660052910052908 1105 38-39 -14.01058201058201 1105 40-41 -13.178571428571429 1105 42-43 -13.293650793650794 1105 44-45 -13.146164021164017 1105 46-47 -13.149470899470899 1105 48-49 -12.892195767195767 1105 50-51 -12.814814814814813 1105 52-53 -11.780423280423278 1105 54-55 -11.625661375661377 1105 56-57 -11.681878306878307 1105 58-59 -12.11441798941799 1105 60-61 -11.756613756613756 1105 62-63 -11.66335978835979 1105 64-65 -11.228835978835976 1105 66-67 -11.178571428571429 1105 68-69 -10.867724867724867 1105 70-71 -10.388888888888888 1105 72-73 -10.356481481481481 1105 74-75 -9.632936507936511 1105 76-77 -9.838624338624337 1105 78-79 -9.703703703703704 1105 80-81 -9.26719576719577 1105 82-83 -9.451058201058203 1105 84-85 -9.412698412698413 1105 86-87 -9.267195767195766 1105 88-89 -8.982804232804233 1105 90-91 -8.72089947089947 1105 92-93 -8.713624338624339 1105 94-95 -8.351190476190476 1105 96-97 -7.777116402116402 1105 98-99 -7.882275132275133 1105 100 -7.777777777777777 1106 1 -14.789682539682545 1106 2 -14.11111111111111 1106 3 -14.23941798941799 1106 4 -16.694444444444443 1106 5 -16.288359788359784 1106 6 -16.089947089947092 1106 7 -16.77777777777778 1106 8 -15.670634920634924 1106 9 -15.841269841269842 1106 10-11 -16.670634920634924 1106 12-13 -17.131613756613756 1106 14-15 -17.060185185185187 1106 16-17 -16.521164021164015 1106 18-19 -16.452380952380953 1106 20-21 -16.41137566137566 1106 22-23 -16.254629629629626 1106 24-25 -15.798280423280428 1106 26-27 -14.581349206349202 1106 28-29 -14.444444444444443 1106 30-31 -14.764550264550266 1106 32-33 -14.55357142857143 1106 34-35 -13.991402116402117 1106 36-37 -13.660052910052908 1106 38-39 -14.01058201058201 1106 40-41 -13.178571428571429 1106 42-43 -13.293650793650794 1106 44-45 -13.146164021164017 1106 46-47 -13.149470899470899 1106 48-49 -12.892195767195767 1106 50-51 -12.814814814814813 1106 52-53 -11.780423280423278 1106 54-55 -11.625661375661377 1106 56-57 -11.681878306878307 1106 58-59 -12.11441798941799 1106 60-61 -11.756613756613756 1106 62-63 -11.66335978835979 1106 64-65 -11.228835978835976 1106 66-67 -11.178571428571429 1106 68-69 -10.867724867724867 1106 70-71 -10.388888888888888 1106 72-73 -10.356481481481481 1106 74-75 -9.632936507936511 1106 76-77 -9.838624338624337 1106 78-79 -9.703703703703704 1106 80-81 -9.26719576719577 1106 82-83 -9.451058201058203 1106 84-85 -9.412698412698413 1106 86-87 -9.267195767195766 1106 88-89 -8.982804232804233 1106 90-91 -8.72089947089947 1106 92-93 -8.713624338624339 1106 94-95 -8.351190476190476 1106 96-97 -7.777116402116402 1106 98-99 -7.882275132275133 1106 100 -7.777777777777777 1107 1 13.710317460317455 1107 2 -0.11111111111111072 1107 3 9.76058201058201 1107 4 12.305555555555557 1107 5 4.211640211640216 1107 6 13.410052910052908 1107 7 16.22222222222222 1107 8 18.329365079365076 1107 9 17.158730158730158 1107 10-11 11.079365079365076 1107 12-13 10.618386243386244 1107 14-15 14.189814814814813 1107 16-17 14.228835978835985 1107 18-19 13.297619047619047 1107 20-21 15.838624338624339 1107 22-23 9.745370370370374 1107 24-25 12.951719576719572 1107 26-27 17.918650793650798 1107 28-29 14.805555555555557 1107 30-31 16.235449735449734 1107 32-33 20.19642857142857 1107 34-35 19.508597883597883 1107 36-37 20.339947089947092 1107 38-39 15.73941798941799 1107 40-41 19.07142857142857 1107 42-43 22.956349206349206 1107 44-45 22.60383597883598 1107 46-47 24.6005291005291 1107 48-49 24.107804232804234 1107 50-51 17.185185185185187 1107 52-53 21.96957671957672 1107 54-55 24.124338624338623 1107 56-57 18.568121693121693 1107 58-59 18.88558201058201 1107 60-61 17.743386243386244 1107 62-63 16.08664021164021 1107 64-65 16.021164021164026 1107 66-67 15.071428571428571 1107 68-69 21.132275132275133 1107 70-71 19.111111111111114 1107 72-73 17.64351851851852 1107 74-75 8.367063492063489 1107 76-77 17.161375661375665 1107 78-79 18.796296296296298 1107 80-81 20.48280423280423 1107 82-83 19.298941798941797 1107 84-85 21.83730158730159 1107 86-87 19.982804232804234 1107 88-89 18.767195767195766 1107 90-91 3.52910052910053 1107 92-93 4.786375661375661 1107 94-95 6.398809523809524 1107 96-97 -2.027116402116402 1107 98-99 3.617724867724867 1107 100 7.222222222222223 1108 1 -6.123015873015879 1108 2 -9.444444444444443 1108 3 -4.906084656084657 1108 4 -9.027777777777777 1108 5 -13.621693121693117 1108 6 -5.089947089947092 1108 7 -6.444444444444445 1108 8 -10.670634920634924 1108 9 -8.174603174603176 1108 10-11 -9.170634920634924 1108 12-13 -6.131613756613756 1108 14-15 -5.060185185185187 1108 16-17 -5.187830687830683 1108 18-19 -10.285714285714285 1108 20-21 -8.411375661375661 1108 22-23 -6.754629629629626 1108 24-25 -9.464947089947094 1108 26-27 -9.414682539682536 1108 28-29 -6.944444444444443 1108 30-31 -6.931216931216934 1108 32-33 -3.5535714285714306 1108 34-35 -2.6580687830687832 1108 36-37 -4.99338624338624 1108 38-39 -4.510582010582009 1108 40-41 -5.678571428571429 1108 42-43 -4.293650793650794 1108 44-45 -2.479497354497349 1108 46-47 -4.982804232804231 1108 48-49 -2.8921957671957674 1108 50-51 -2.1481481481481453 1108 52-53 -1.947089947089946 1108 54-55 -6.792328042328043 1108 56-57 -1.848544973544973 1108 58-59 -2.7810846560846567 1108 60-61 -3.923280423280424 1108 62-63 -0.9966931216931219 1108 64-65 -4.895502645502644 1108 66-67 -5.845238095238096 1108 68-69 -2.034391534391535 1108 70-71 -2.0555555555555554 1108 72-73 -6.023148148148148 1108 74-75 -1.9662698412698454 1108 76-77 -3.1719576719576708 1108 78-79 -4.037037037037037 1108 80-81 -9.26719576719577 1108 82-83 -9.451058201058203 1108 84-85 -9.412698412698413 1108 86-87 -9.267195767195766 1108 88-89 -8.982804232804233 1108 90-91 -8.72089947089947 1108 92-93 -8.713624338624339 1108 94-95 -8.351190476190476 1108 96-97 -7.777116402116402 1108 98-99 -7.882275132275133 1108 100 -7.777777777777777 1109 1 -0.28968253968254487 1109 2 0.3888888888888893 1109 3 0.26058201058200936 1109 4 -0.19444444444444287 1109 5 0.21164021164021563 1109 6 0.410052910052908 1109 7 0.7222222222222214 1109 8 0.8293650793650755 1109 9 2.658730158730158 1109 10-11 0.8293650793650755 1109 12-13 1.3683862433862437 1109 14-15 0.6898148148148131 1109 16-17 2.228835978835985 1109 18-19 1.7976190476190474 1109 20-21 3.0886243386243386 1109 22-23 1.4953703703703738 1109 24-25 3.4517195767195723 1109 26-27 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2.0171957671957674 2216 90-91 2.27910052910053 2216 92-93 2.2863756613756614 2216 94-95 2.6488095238095237 2216 96-97 3.222883597883598 2216 98-99 3.117724867724867 2216 100 3.222222222222223 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 596.0 3 40.0 4 28.0 5 35.0 6 36.0 7 23.0 8 23.0 9 20.0 10 19.0 11 17.0 12 21.0 13 17.0 14 13.0 15 13.0 16 21.0 17 12.0 18 20.0 19 14.0 20 9.0 21 10.0 22 15.0 23 19.0 24 19.0 25 23.0 26 31.0 27 15.0 28 24.0 29 23.0 30 27.0 31 30.0 32 37.0 33 48.0 34 38.0 35 56.0 36 41.0 37 53.0 38 31.0 39 8.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.7596848325673 20.420223243598162 12.672357189757058 25.147734734077474 2 16.874589625738672 22.980958634274458 35.58765594221931 24.556795797767563 3 19.908015768725363 26.938239159001316 26.084099868593956 27.06964520367937 4 16.754270696452036 17.54270696452037 33.31143232588699 32.3915900131406 5 13.65725541694025 37.88575180564675 32.17334208798424 16.283650689428757 6 29.94090610636901 37.03217334208798 17.662508207485224 15.364412344057781 7 28.890347997373606 32.239001969796455 20.81418253447144 18.056467498358504 8 24.622455679579776 36.50689428759028 21.273801707156927 17.596848325673015 9 26.59225213394616 19.63230466185161 20.288903479973737 33.4865397242285 10-11 24.294156270518712 27.839789888378203 25.673013788575183 22.193040052527905 12-13 24.819435325016414 24.852265265922522 26.23112278397899 24.097176625082074 14-15 23.571897570584373 27.839789888378203 23.112278397898883 25.47603414313854 16-17 23.80170715692712 29.218647406434666 24.392646093237033 22.58699934340118 18-19 24.917925147734735 26.986211424819434 25.443204202232433 22.652659225213394 20-21 23.604727511490477 27.675640183847673 25.31188443860801 23.40774786605384 22-23 23.96585686145765 27.380170715692714 24.622455679579776 24.03151674326986 24-25 23.99081063340991 26.87889727600919 23.268788972760092 25.861503117820806 26-27 25.21339461588969 26.263952724885097 24.556795797767563 23.96585686145765 28-29 26.0498687664042 26.279527559055115 23.589238845144354 24.081364829396325 30-31 24.49113591595535 26.85489166119501 23.96585686145765 24.688115561391992 32-33 23.03905480800788 27.075812274368232 23.72825730226452 26.156875615359372 34-35 27.511490479317136 24.294156270518712 24.06434668417597 24.130006565988182 36-37 26.189694781752543 24.187725631768952 23.498523137512308 26.124056448966197 38-39 22.809320643255663 27.502461437479486 23.957991467016736 25.73022645224811 40-41 24.745651460452905 25.17230062356416 23.400065638332787 26.681982277650146 42-43 23.637557452396585 24.556795797767563 25.508864084044646 26.296782665791202 44-45 24.622455679579776 24.359816152330925 25.673013788575183 25.344714379514116 46-47 24.532019704433498 26.00985221674877 23.842364532019705 25.615763546798032 48-49 23.258869908015768 25.0 24.277266754270695 27.463863337713533 50-51 23.71879106438896 27.595269382391592 24.96714848883049 23.71879106438896 52-53 24.342969776609724 27.135348226018397 24.178712220762154 24.342969776609724 54-55 22.601839684625492 27.102496714848883 24.11300919842313 26.182654402102497 56-57 24.67148488830486 25.295663600525625 25.459921156373195 24.57293035479632 58-59 25.854139290407357 24.57293035479632 24.540078843626805 25.03285151116951 60-61 25.459921156373195 24.441524310118265 24.375821287779235 25.7227332457293 62-63 25.295663600525625 27.23390275952694 23.390275952693823 24.080157687253614 64-65 24.11300919842313 26.872536136662283 23.68593955321945 25.32851511169514 66-67 25.418719211822662 26.403940886699505 22.758620689655174 25.418719211822662 68-69 26.23112278397899 25.31188443860801 23.604727511490477 24.852265265922522 70-71 25.508864084044646 25.08207485226527 23.703217334208798 25.705843729481288 72-73 24.556795797767563 25.869993434011818 24.7537754432042 24.819435325016414 74-75 26.822061720288904 24.655285620485884 22.35718975705844 26.165462902166777 76-77 27.708470124753777 24.78660538411031 23.013788575180566 24.49113591595535 78-79 27.3145108338805 24.720945502298093 24.162836506894287 23.80170715692712 80-81 25.31188443860801 25.377544320420224 24.261326329612608 25.04924491135916 82-83 26.59225213394616 25.673013788575183 23.210768220617204 24.52396585686146 84-85 27.511490479317136 23.96585686145765 24.359816152330925 24.162836506894287 86-87 25.869993434011818 24.32698621142482 23.473407747866055 26.329612606697307 88-89 26.85489166119501 25.08207485226527 23.40774786605384 24.655285620485884 90-91 25.33639645553003 26.189694781752543 24.61437479488021 23.85953396783722 92-93 26.509186351706038 24.409448818897637 23.9501312335958 25.131233595800524 94-95 24.835958005249346 24.376640419947506 25.098425196850393 25.688976377952756 96-97 26.31233595800525 23.68766404199475 23.72047244094488 26.279527559055115 98-99 26.443569553805773 25.26246719160105 23.12992125984252 25.164041994750658 100 25.984251968503933 25.0 24.278215223097114 24.73753280839895 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1.0 1 1.0 2 1.0 3 0.5 4 0.0 5 0.5 6 0.5 7 0.0 8 1.0 9 1.0 10 0.0 11 0.5 12 0.5 13 0.0 14 0.0 15 0.0 16 1.0 17 1.5 18 1.0 19 1.5 20 1.0 21 0.0 22 1.0 23 2.5 24 2.0 25 3.5 26 5.5 27 5.5 28 9.0 29 13.0 30 17.0 31 18.5 32 16.0 33 20.0 34 21.5 35 24.5 36 32.0 37 34.0 38 34.5 39 38.0 40 43.0 41 44.0 42 46.0 43 42.5 44 40.0 45 45.5 46 48.0 47 41.0 48 38.5 49 40.0 50 45.5 51 49.0 52 42.0 53 42.0 54 44.0 55 47.5 56 47.0 57 43.5 58 41.0 59 34.5 60 32.5 61 36.0 62 30.5 63 26.0 64 28.0 65 26.0 66 23.0 67 16.5 68 13.0 69 14.0 70 11.0 71 7.0 72 7.5 73 9.5 74 8.5 75 6.5 76 6.0 77 7.5 78 7.5 79 4.0 80 2.0 81 6.5 82 7.0 83 2.5 84 3.0 85 2.5 86 1.0 87 1.0 88 1.5 89 1.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13114754098360656 2 0.13114754098360656 3 0.19672131147540983 4 0.19672131147540983 5 0.13114754098360656 6 0.13114754098360656 7 0.13114754098360656 8 0.13114754098360656 9 0.13114754098360656 10-11 0.13114754098360656 12-13 0.13114754098360656 14-15 0.13114754098360656 16-17 0.13114754098360656 18-19 0.13114754098360656 20-21 0.13114754098360656 22-23 0.13114754098360656 24-25 0.09836065573770492 26-27 0.13114754098360656 28-29 0.06557377049180328 30-31 0.13114754098360656 32-33 0.09836065573770492 34-35 0.13114754098360656 36-37 0.09836065573770492 38-39 0.09836065573770492 40-41 0.09836065573770492 42-43 0.13114754098360656 44-45 0.13114754098360656 46-47 0.16393442622950818 48-49 0.19672131147540983 50-51 0.19672131147540983 52-53 0.19672131147540983 54-55 0.19672131147540983 56-57 0.19672131147540983 58-59 0.19672131147540983 60-61 0.19672131147540983 62-63 0.19672131147540983 64-65 0.19672131147540983 66-67 0.16393442622950818 68-69 0.13114754098360656 70-71 0.13114754098360656 72-73 0.13114754098360656 74-75 0.13114754098360656 76-77 0.13114754098360656 78-79 0.13114754098360656 80-81 0.13114754098360656 82-83 0.13114754098360656 84-85 0.13114754098360656 86-87 0.13114754098360656 88-89 0.13114754098360656 90-91 0.09836065573770492 92-93 0.06557377049180328 94-95 0.06557377049180328 96-97 0.06557377049180328 98-99 0.06557377049180328 100 0.06557377049180328 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1525.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.9344262295082 #Duplication Level Percentage of deduplicated Percentage of total 1 99.93438320209974 99.8688524590164 2 0.06561679790026247 0.13114754098360656 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2 0.13114754098360656 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.03278688524590164 0.0 38-39 0.0 0.0 0.0 0.13114754098360656 0.0 40-41 0.0 0.0 0.0 0.13114754098360656 0.0 42-43 0.0 0.0 0.0 0.13114754098360656 0.0 44-45 0.0 0.0 0.0 0.19672131147540983 0.0 46-47 0.0 0.0 0.0 0.22950819672131148 0.0 48-49 0.0 0.0 0.0 0.36065573770491804 0.0 50-51 0.0 0.0 0.0 0.5245901639344263 0.0 52-53 0.0 0.0 0.0 0.5901639344262295 0.0 54-55 0.0 0.0 0.0 0.7540983606557377 0.0 56-57 0.0 0.0 0.0 0.8852459016393442 0.0 58-59 0.0 0.0 0.0 0.9180327868852459 0.0 60-61 0.0 0.0 0.0 1.081967213114754 0.0 62-63 0.0 0.0 0.0 1.2459016393442623 0.0 64-65 0.0 0.0 0.0 1.2459016393442623 0.0 66-67 0.0 0.0 0.0 1.278688524590164 0.0 68-69 0.0 0.0 0.0 1.5737704918032787 0.0 70-71 0.0 0.0 0.0 1.6721311475409837 0.0 72-73 0.0 0.0 0.0 1.7704918032786885 0.0 74-75 0.0 0.0 0.0 2.0655737704918034 0.0 76-77 0.0 0.0 0.0 2.3278688524590163 0.0 78-79 0.0 0.0 0.0 2.459016393442623 0.0 80-81 0.0 0.0 0.0 2.5245901639344264 0.0 82-83 0.0 0.0 0.0 2.6557377049180326 0.0 84-85 0.0 0.0 0.0 2.918032786885246 0.0 86-87 0.0 0.0 0.0 3.081967213114754 0.0 88 0.0 0.0 0.0 3.1475409836065573 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE