FastQCFastQC Report
Wed 25 May 2016
SRR1032949_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032949_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1525
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCG110.7213114754098361No Hit
TGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACT110.7213114754098361No Hit
ATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAA80.5245901639344263No Hit
CCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCCAAGTCCC80.5245901639344263No Hit
GTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGCCCCTCTA70.45901639344262296No Hit
CGCTACGAGAGTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGC70.45901639344262296No Hit
CAGTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTGTCTACTGTGGGCA70.45901639344262296No Hit
CTTCAGGGGAGATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTG60.39344262295081966No Hit
GTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATC50.32786885245901637No Hit
CTACGAGAGTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCA50.32786885245901637No Hit
GTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGC50.32786885245901637No Hit
GGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGC40.26229508196721313No Hit
ACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGG40.26229508196721313No Hit
CCATGAAGGCCTCGGGCACGCTACGAGAGTACAAGGTAGTGGGTCGCTGC40.26229508196721313No Hit
CCCCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCCAAGTC40.26229508196721313No Hit
GTCCCGCTTCTGGTACTTTGTATCTCAGTTAAAGAAGATGAAGAAGTCTT40.26229508196721313No Hit
TTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTGTCTAC40.26229508196721313No Hit
GGGGTAGGAAATCCTCCAGGATCTTAGGAAATTTCACTCCGCTTCTCCTG40.26229508196721313No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG30.19672131147540983RNA PCR Primer, Index 46 (96% over 28bp)
CTTCTGGTACTTTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGG30.19672131147540983No Hit
GGCCTCGGGCACGCTACGAGAGTACAAGGTAGTGGGTCGCTGCCTGCCCA30.19672131147540983No Hit
CCCACAACATGTACCGGGAATACCGGGACCTGACCACCGCAGGCGCTGTC30.19672131147540983No Hit
TATCAACGCAGAGTACATGGGGCCTTTTGCGGGTGGCGGCGAACGCGGAG30.19672131147540983No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.19672131147540983No Hit
GTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTATGACTCCC30.19672131147540983No Hit
CCCCCAAATGCCACACGCCGCCCCTCTACCGCATGCGAATCTTTGCGCCT30.19672131147540983No Hit
GAGATACGGAGGCCCGGTCAGACTCTCCCTCTGCACCTCAATGGAAACGA30.19672131147540983No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG30.19672131147540983RNA PCR Primer, Index 27 (96% over 30bp)
GAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTATGACT30.19672131147540983No Hit
TCACAGCGCTGCGCCGCACAGTGCCCCCCGCTGTCACTGGGATCACCTTC20.13114754098360656No Hit
CCCTCAGCCCTCGCCATCATGGAAAATGCCAATGTTCTGGCCCGTTATGC20.13114754098360656No Hit
GTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTGTCTACTGTGGGCAGG20.13114754098360656No Hit
GCCTCAGAGGCTGCTGTCAGCGCATTGATCCGCACCATAGCTGGCTCCCA20.13114754098360656No Hit
GCCTGAGATCCTCCCTGATGGGGACCATGACTTGAAGCGCTGCCAGTATG20.13114754098360656No Hit
GATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGA20.13114754098360656No Hit
AGTGTACTTGGCGTGCTGGCTGATTTCAATTTTCTTCACCATTTTCCGGA20.13114754098360656No Hit
GGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTG20.13114754098360656No Hit
ATGCCACACGCCGCCCCTCTACCGCATGCGAATCTTTGCGCCTAATCATG20.13114754098360656No Hit
GTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTT20.13114754098360656No Hit
GTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCT20.13114754098360656No Hit
ACATTCCACTCTCCTGACAGATGGAGTTAATATCAGCTCCTGAAATCTTA20.13114754098360656No Hit
CGAGAGTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACAC20.13114754098360656No Hit
ATCATGTCGTCGCCAAGTCCCGCTTCTGGTACTTTGTATCTCAGTTAAAG20.13114754098360656No Hit
GTACATGGGGTCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATG20.13114754098360656No Hit
AGATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAG20.13114754098360656No Hit
CCCCGGAACACGCCTCCGCCTGAGCCTCGGGCCCGTCGGGATCTGCCTTT20.13114754098360656No Hit
GTATCAACGCAGAGTACATGGGGCCTTTTGCGGGTGGCGGCGAACGCGGA20.13114754098360656No Hit
TCTTTGCGCCTAATCATGTCGTCGCCAAGTCCCGCTTCTGGTACTTTGTA20.13114754098360656No Hit
CTACTGGTCTTTCAACTCCTTCAGTCTTCTGATGGCGGACTTTACCGTGA20.13114754098360656No Hit
GTACTGGACTGTGTGCCAGAGGAGAGGCGTAAACTGATTGTGGCATATGT20.13114754098360656No Hit
CTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTATGACTCCCGGAGCGG20.13114754098360656No Hit
GTACCTGGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAA20.13114754098360656No Hit
ACCGGGACCTGACCACCGCAGGCGCTGTCACCCAGTGCTACCGAGACATG20.13114754098360656No Hit
CTGCCTGCCCACCCCCAAATGCCACACGCCGCCCCTCTACCGCATGCGAA20.13114754098360656No Hit
TAATCATGTCGTCGCCAAGTCCCGCTTCTGGTACTTTGTATCTCAGTTAA20.13114754098360656No Hit
GTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTGTCTACTG20.13114754098360656No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.13114754098360656No Hit
GTGGAGGGGGCAGGACCTCCTGCTGGTGCAGCACCAGCTGCTGGAGCAGG20.13114754098360656No Hit
ATAATATCCCGGGGCAAACCGGAGAATTTGGCAGTCCGATTGGGGGGGGG20.13114754098360656No Hit
CTGTAGACCTGGTCTTTTTCCCCCCAGTTTTTTCTTTTTTACATTCTGGG20.13114754098360656No Hit
GTCGTCGCCAAGTCCCGCTTCTGGTACTTTGTATCTCAGTTAAAGAAGAT20.13114754098360656No Hit
TAGTCATCCCACTCGGCCTGGTAACACGTGCCAGCCACCGGGGCCCTGAG20.13114754098360656No Hit
TGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGC20.13114754098360656No Hit
ACATGGGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAAG20.13114754098360656No Hit
GTCTTCAGGGGAGATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCC20.13114754098360656No Hit
TGTCCATCTTGCAGGTGGCTCTTCCTCCACATCACGACTGGGGTCTCGAA20.13114754098360656No Hit
GGCACCCACAACATGTACCGGGAATACCGGGACCTGACCACCGCAGGCGC20.13114754098360656No Hit
GGCAAGAAGGGTGTGCTGTTTGGAGTTCCTGGGGCCTTCACCCCTGGATG20.13114754098360656No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)