##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032949_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1525 Sequences flagged as poor quality 0 Sequence length 100 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.226885245901638 31.0 31.0 34.0 28.0 34.0 2 31.28983606557377 31.0 31.0 34.0 28.0 34.0 3 31.33049180327869 31.0 31.0 34.0 28.0 34.0 4 35.278688524590166 35.0 35.0 37.0 33.0 37.0 5 35.10622950819672 35.0 35.0 37.0 33.0 37.0 6 35.081311475409834 35.0 35.0 37.0 32.0 37.0 7 34.8944262295082 35.0 35.0 37.0 32.0 37.0 8 34.79409836065574 35.0 35.0 37.0 32.0 37.0 9 36.284590163934425 38.0 35.0 39.0 32.0 39.0 10-11 36.34655737704918 37.0 35.0 39.0 32.0 39.0 12-13 36.30196721311475 37.0 35.0 39.0 32.0 39.0 14-15 37.32327868852459 39.0 36.0 40.0 32.0 41.0 16-17 37.20360655737704 38.5 36.0 40.0 32.0 41.0 18-19 37.28229508196721 39.0 36.0 40.0 31.5 41.0 20-21 37.268524590163935 39.0 36.0 40.0 31.5 41.0 22-23 37.09704918032787 39.0 36.0 40.0 31.0 41.0 24-25 37.21836065573771 39.0 36.0 40.0 31.0 41.0 26-27 36.93901639344262 38.0 35.5 40.0 31.0 41.0 28-29 36.89606557377049 38.5 35.5 40.0 30.0 41.0 30-31 36.68327868852459 38.0 35.0 40.0 30.0 41.0 32-33 36.55803278688525 38.0 35.0 40.0 30.0 41.0 34-35 36.281639344262295 38.0 35.0 40.0 30.0 41.0 36-37 36.02393442622951 38.0 34.5 40.0 30.0 41.0 38-39 36.03672131147541 38.0 34.5 40.0 30.0 41.0 40-41 35.5272131147541 38.0 34.5 40.0 27.5 41.0 42-43 35.41213114754098 38.0 34.0 40.0 27.0 41.0 44-45 35.213114754098356 38.0 33.0 40.0 27.5 41.0 46-47 35.01081967213115 37.5 33.5 40.0 27.5 41.0 48-49 34.68786885245902 37.0 33.0 40.0 27.0 41.0 50-51 34.522295081967215 37.0 33.0 40.0 26.5 41.0 52-53 34.02098360655738 36.5 33.0 39.5 24.0 41.0 54-55 33.91016393442623 36.0 33.0 39.0 25.5 41.0 56-57 33.60622950819672 36.0 32.0 39.5 23.5 41.0 58-59 33.334754098360655 35.0 32.0 39.0 23.5 41.0 60-61 32.93901639344262 35.0 31.5 39.0 22.5 40.0 62-63 32.736393442622955 35.0 31.0 38.5 23.0 40.0 64-65 32.40688524590164 35.0 31.0 38.0 22.5 40.0 66-67 31.988196721311475 34.5 31.0 37.5 22.0 40.0 68-69 31.887213114754097 34.5 31.0 37.0 22.0 39.5 70-71 31.44327868852459 34.0 30.0 36.0 20.5 39.0 72-73 31.094754098360653 34.0 30.0 36.0 20.0 39.0 74-75 30.808524590163934 34.0 29.5 35.5 20.0 38.0 76-77 30.479344262295083 34.0 30.0 35.0 20.0 37.5 78-79 30.177704918032788 34.0 29.0 35.0 18.5 37.0 80-81 29.964262295081966 34.0 29.0 35.0 17.5 36.5 82-83 29.66655737704918 33.5 29.0 35.0 15.0 36.0 84-85 29.42327868852459 33.5 29.0 35.0 11.0 36.0 86-87 29.34065573770492 33.0 29.0 35.0 12.5 35.0 88-89 29.14327868852459 33.0 29.0 35.0 10.0 35.0 90-91 29.017704918032784 33.0 29.0 35.0 7.0 35.0 92-93 28.937049180327868 33.5 29.0 35.0 2.0 35.0 94-95 28.89049180327869 33.5 29.0 35.0 2.0 35.0 96-97 28.478032786885244 33.0 29.0 35.0 2.0 35.0 98-99 28.382950819672132 33.0 29.0 35.0 2.0 35.0 100 28.289180327868852 33.0 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1102 1 2.3492063492063444 1102 2 2.113756613756607 1102 3 2.0079365079365097 1102 4 1.2208994708994823 1102 5 1.2539682539682602 1102 6 1.6613756613756507 1102 7 1.650793650793652 1102 8 2.0873015873015888 1102 9 2.1865079365079296 1102 10-11 -0.5972222222222214 1102 12-13 2.3148148148148096 1102 14-15 0.886243386243379 1102 16-17 1.0886243386243493 1102 18-19 1.628968253968253 1102 20-21 1.9431216931216966 1102 22-23 3.3115079365079296 1102 24-25 -2.283730158730151 1102 26-27 1.9113756613756507 1102 28-29 2.460317460317455 1102 30-31 2.0019841269841336 1102 32-33 0.8220899470899425 1102 34-35 0.1203703703703809 1102 36-37 2.5707671957672034 1102 38-39 3.7109788359788354 1102 40-41 3.988756613756614 1102 42-43 4.074735449735456 1102 44-45 4.513888888888893 1102 46-47 2.978835978835974 1102 48-49 5.052248677248677 1102 50-51 4.604497354497354 1102 52-53 3.8134920634920633 1102 54-55 2.56216931216931 1102 56-57 2.5191798941798993 1102 58-59 1.2440476190476133 1102 60-61 1.058201058201064 1102 62-63 1.584656084656082 1102 64-65 1.6448412698412795 1102 66-67 1.9609788359788354 1102 68-69 2.0165343915343925 1102 70-71 2.8802910052909922 1102 72-73 2.835317460317455 1102 74-75 2.8941798941798993 1102 76-77 2.960317460317455 1102 78-79 3.7817460317460316 1102 80-81 4.3220899470899425 1102 82-83 4.074074074074073 1102 84-85 3.470899470899475 1102 86-87 4.270502645502649 1102 88-89 4.185185185185183 1102 90-91 4.300925925925931 1102 92-93 4.218253968253972 1102 94-95 4.652777777777779 1102 96-97 4.545634920634917 1102 98-99 1.1388888888888857 1102 100 0.7460317460317505 1103 1 2.3492063492063444 1103 2 -0.8862433862433932 1103 3 2.0079365079365097 1103 4 1.2208994708994823 1103 5 1.2539682539682602 1103 6 1.6613756613756507 1103 7 1.650793650793652 1103 8 2.0873015873015888 1103 9 2.1865079365079296 1103 10-11 2.4027777777777786 1103 12-13 2.3148148148148096 1103 14-15 1.886243386243379 1103 16-17 1.5886243386243493 1103 18-19 3.128968253968253 1103 20-21 1.9431216931216966 1103 22-23 2.3115079365079296 1103 24-25 1.716269841269849 1103 26-27 2.9113756613756507 1103 28-29 0.9603174603174551 1103 30-31 3.0019841269841336 1103 32-33 3.8220899470899425 1103 34-35 3.620370370370381 1103 36-37 4.070767195767203 1103 38-39 2.7109788359788354 1103 40-41 3.488756613756614 1103 42-43 1.5747354497354564 1103 44-45 2.013888888888893 1103 46-47 -1.0211640211640258 1103 48-49 -0.4477513227513228 1103 50-51 -0.3955026455026456 1103 52-53 -0.18650793650793673 1103 54-55 0.062169312169309876 1103 56-57 -3.9808201058201007 1103 58-59 0.7440476190476133 1103 60-61 1.058201058201064 1103 62-63 0.584656084656082 1103 64-65 1.6448412698412795 1103 66-67 1.9609788359788354 1103 68-69 2.0165343915343925 1103 70-71 2.8802910052909922 1103 72-73 2.835317460317455 1103 74-75 2.8941798941798993 1103 76-77 2.460317460317455 1103 78-79 3.7817460317460316 1103 80-81 4.3220899470899425 1103 82-83 4.074074074074073 1103 84-85 3.470899470899475 1103 86-87 3.270502645502649 1103 88-89 4.185185185185183 1103 90-91 4.300925925925931 1103 92-93 3.718253968253972 1103 94-95 4.152777777777779 1103 96-97 5.045634920634917 1103 98-99 3.6388888888888857 1103 100 4.7460317460317505 1104 1 0.3492063492063444 1104 2 0.11375661375660684 1104 3 0.007936507936509685 1104 4 0.554232804232818 1104 5 -0.07936507936507553 1104 6 -2.3386243386243493 1104 7 0.31746031746031633 1104 8 1.4206349206349245 1104 9 2.1865079365079296 1104 10-11 1.7361111111111143 1104 12-13 0.48148148148148096 1104 14-15 0.21957671957670755 1104 16-17 -2.7447089947089793 1104 18-19 1.4623015873015888 1104 20-21 0.7764550264550323 1104 22-23 0.47817460317460103 1104 24-25 2.216269841269849 1104 26-27 2.4113756613756507 1104 28-29 2.960317460317455 1104 30-31 1.3353174603174693 1104 32-33 -2.011243386243386 1104 34-35 0.1203703703703809 1104 36-37 -1.595899470899468 1104 38-39 -1.1223544973545003 1104 40-41 1.988756613756614 1104 42-43 1.5747354497354564 1104 44-45 0.6805555555555571 1104 46-47 2.1455026455026456 1104 48-49 1.5522486772486772 1104 50-51 2.6044973544973544 1104 52-53 3.646825396825392 1104 54-55 3.2288359788359813 1104 56-57 4.852513227513228 1104 58-59 3.7440476190476133 1104 60-61 3.3915343915343925 1104 62-63 3.584656084656082 1104 64-65 2.6448412698412795 1104 66-67 1.9609788359788354 1104 68-69 1.5165343915343925 1104 70-71 2.8802910052909922 1104 72-73 3.0019841269841265 1104 74-75 1.727513227513228 1104 76-77 2.6269841269841265 1104 78-79 3.6150793650793602 1104 80-81 3.988756613756607 1104 82-83 3.4074074074074012 1104 84-85 4.304232804232804 1104 86-87 4.4371693121693205 1104 88-89 3.8518518518518476 1104 90-91 4.300925925925931 1104 92-93 3.718253968253972 1104 94-95 3.6527777777777786 1104 96-97 4.045634920634917 1104 98-99 3.9722222222222143 1104 100 4.412698412698415 1105 1 -3.6507936507936556 1105 2 -0.8862433862433932 1105 3 -3.9920634920634903 1105 4 -5.279100529100518 1105 5 -0.7460317460317398 1105 6 -1.3386243386243493 1105 7 -0.34920634920634797 1105 8 -0.9126984126984112 1105 9 -0.8134920634920704 1105 10-11 -0.34722222222222143 1105 12-13 -0.18518518518519045 1105 14-15 -0.11375661375662105 1105 16-17 -1.9113756613756507 1105 18-19 -0.12103174603174693 1105 20-21 -1.0568783068783034 1105 22-23 -1.4384920634920704 1105 24-25 1.716269841269849 1105 26-27 -5.338624338624349 1105 28-29 -4.039682539682545 1105 30-31 -1.7480158730158664 1105 32-33 1.0720899470899425 1105 34-35 -0.1296296296296191 1105 36-37 -10.179232804232797 1105 38-39 -0.2890211640211646 1105 40-41 -0.26124338624338606 1105 42-43 0.07473544973545643 1105 44-45 -1.9861111111111072 1105 46-47 -2.021164021164026 1105 48-49 0.8022486772486772 1105 50-51 -0.8955026455026456 1105 52-53 -7.436507936507937 1105 54-55 -4.68783068783069 1105 56-57 1.2691798941798993 1105 58-59 0.2440476190476133 1105 60-61 2.808201058201064 1105 62-63 1.834656084656082 1105 64-65 -1.8551587301587205 1105 66-67 0.21097883597883538 1105 68-69 -1.2334656084656075 1105 70-71 -4.119708994709008 1105 72-73 -1.6646825396825449 1105 74-75 0.3941798941798993 1105 76-77 -0.7896825396825449 1105 78-79 -0.46825396825396837 1105 80-81 0.07208994708994254 1105 82-83 1.3240740740740726 1105 84-85 -2.029100529100525 1105 86-87 -1.4794973544973509 1105 88-89 1.1851851851851833 1105 90-91 -2.699074074074069 1105 92-93 1.468253968253972 1105 94-95 3.1527777777777786 1105 96-97 1.2956349206349174 1105 98-99 0.38888888888888573 1105 100 3.2460317460317505 1106 1 -2.1507936507936556 1106 2 0.11375661375660684 1106 3 0.5079365079365097 1106 4 0.22089947089948225 1106 5 0.25396825396826017 1106 6 0.6613756613756507 1106 7 0.650793650793652 1106 8 1.0873015873015888 1106 9 1.1865079365079296 1106 10-11 -1.0972222222222214 1106 12-13 1.8148148148148096 1106 14-15 2.636243386243379 1106 16-17 2.0886243386243493 1106 18-19 -2.121031746031747 1106 20-21 1.4431216931216966 1106 22-23 1.0615079365079296 1106 24-25 0.46626984126984894 1106 26-27 1.9113756613756507 1106 28-29 1.4603174603174551 1106 30-31 2.2519841269841336 1106 32-33 2.3220899470899425 1106 34-35 3.620370370370381 1106 36-37 2.0707671957672034 1106 38-39 2.2109788359788354 1106 40-41 3.238756613756614 1106 42-43 1.3247354497354564 1106 44-45 2.263888888888893 1106 46-47 3.228835978835974 1106 48-49 3.302248677248677 1106 50-51 1.6044973544973544 1106 52-53 0.8134920634920633 1106 54-55 2.06216931216931 1106 56-57 4.019179894179899 1106 58-59 2.2440476190476133 1106 60-61 1.558201058201064 1106 62-63 2.584656084656082 1106 64-65 1.8948412698412795 1106 66-67 4.210978835978835 1106 68-69 4.5165343915343925 1106 70-71 1.3802910052909922 1106 72-73 2.585317460317455 1106 74-75 4.144179894179899 1106 76-77 3.460317460317455 1106 78-79 -1.2182539682539684 1106 80-81 2.3220899470899425 1106 82-83 1.0740740740740726 1106 84-85 -2.029100529100525 1106 86-87 2.020502645502649 1106 88-89 -3.3148148148148167 1106 90-91 0.3009259259259309 1106 92-93 -0.5317460317460281 1106 94-95 1.6527777777777786 1106 96-97 3.2956349206349174 1106 98-99 3.3888888888888857 1106 100 4.7460317460317505 1107 1 -3.6507936507936556 1107 2 -1.8862433862433932 1107 3 -1.4920634920634903 1107 4 -1.2791005291005177 1107 5 -2.24603174603174 1107 6 -3.8386243386243493 1107 7 -8.849206349206348 1107 8 -0.41269841269841123 1107 9 -4.31349206349207 1107 10-11 -3.0972222222222214 1107 12-13 -3.4351851851851904 1107 14-15 -9.863756613756621 1107 16-17 -4.161375661375651 1107 18-19 -1.371031746031747 1107 20-21 -9.306878306878303 1107 22-23 -7.93849206349207 1107 24-25 -7.783730158730151 1107 26-27 -4.588624338624349 1107 28-29 -8.789682539682545 1107 30-31 -6.748015873015866 1107 32-33 -10.927910052910057 1107 34-35 -12.129629629629619 1107 36-37 -0.42923280423279664 1107 38-39 1.7109788359788354 1107 40-41 -1.011243386243386 1107 42-43 2.5747354497354564 1107 44-45 3.513888888888893 1107 46-47 -1.0211640211640258 1107 48-49 -2.197751322751323 1107 50-51 -2.6455026455026456 1107 52-53 -7.436507936507937 1107 54-55 -1.1878306878306901 1107 56-57 -6.230820105820101 1107 58-59 2.4940476190476133 1107 60-61 -7.691798941798936 1107 62-63 -10.415343915343918 1107 64-65 -4.1051587301587205 1107 66-67 -0.03902116402116462 1107 68-69 -3.9834656084656075 1107 70-71 -0.6197089947090078 1107 72-73 -5.664682539682545 1107 74-75 -5.855820105820101 1107 76-77 -13.539682539682545 1107 78-79 -11.718253968253968 1107 80-81 -13.427910052910057 1107 82-83 -14.425925925925927 1107 84-85 -14.779100529100525 1107 86-87 -14.22949735449735 1107 88-89 -13.314814814814817 1107 90-91 -12.949074074074069 1107 92-93 -12.781746031746028 1107 94-95 -12.597222222222221 1107 96-97 -11.954365079365083 1107 98-99 -12.361111111111114 1107 100 -12.25396825396825 1108 1 1.3492063492063444 1108 2 1.1137566137566068 1108 3 1.0079365079365097 1108 4 -0.11243386243385345 1108 5 0.5873015873015959 1108 6 -2.0052910052910136 1108 7 0.31746031746031633 1108 8 1.0873015873015888 1108 9 0.8531746031745939 1108 10-11 0.40277777777777857 1108 12-13 -1.6851851851851904 1108 14-15 -2.4470899470899568 1108 16-17 0.5886243386243493 1108 18-19 1.128968253968253 1108 20-21 0.609788359788368 1108 22-23 -0.02182539682539897 1108 24-25 0.7162698412698489 1108 26-27 -2.0886243386243493 1108 28-29 -0.03968253968254487 1108 30-31 0.5019841269841336 1108 32-33 0.15542328042327114 1108 34-35 1.120370370370381 1108 36-37 0.5707671957672034 1108 38-39 0.8776455026455068 1108 40-41 1.488756613756614 1108 42-43 1.0747354497354564 1108 44-45 2.3472222222222214 1108 46-47 0.14550264550264558 1108 48-49 -1.114417989417987 1108 50-51 0.2711640211640187 1108 52-53 1.6468253968253919 1108 54-55 1.0621693121693099 1108 56-57 3.852513227513228 1108 58-59 3.7440476190476133 1108 60-61 4.058201058201064 1108 62-63 1.584656084656082 1108 64-65 3.311507936507944 1108 66-67 3.794312169312164 1108 68-69 3.183201058201064 1108 70-71 4.713624338624328 1108 72-73 -0.33134920634920917 1108 74-75 -0.43915343915343286 1108 76-77 1.6269841269841265 1108 78-79 -1.7182539682539684 1108 80-81 1.6554232804232711 1108 82-83 2.240740740740744 1108 84-85 2.470899470899475 1108 86-87 1.6038359788359777 1108 88-89 2.018518518518519 1108 90-91 1.800925925925931 1108 92-93 2.718253968253972 1108 94-95 2.819444444444443 1108 96-97 1.7123015873015817 1108 98-99 2.6388888888888857 1108 100 3.7460317460317505 1109 1 0.8492063492063444 1109 2 0.6137566137566068 1109 3 0.5079365079365097 1109 4 0.22089947089948225 1109 5 1.2539682539682602 1109 6 1.6613756613756507 1109 7 1.650793650793652 1109 8 1.0873015873015888 1109 9 2.1865079365079296 1109 10-11 2.4027777777777786 1109 12-13 2.3148148148148096 1109 14-15 3.136243386243379 1109 16-17 3.0886243386243493 1109 18-19 2.878968253968253 1109 20-21 1.1931216931216966 1109 22-23 2.3115079365079296 1109 24-25 2.466269841269849 1109 26-27 -2.5886243386243493 1109 28-29 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2216 56-57 -2.3141534391534364 2216 58-59 -5.089285714285722 2216 60-61 -1.1084656084656075 2216 62-63 -0.915343915343918 2216 64-65 -2.0218253968253848 2216 66-67 -5.039021164021165 2216 68-69 -1.8167989417989432 2216 70-71 -2.45304232804234 2216 72-73 -0.9980158730158806 2216 74-75 -1.272486772486765 2216 76-77 0.12698412698412653 2216 78-79 0.11507936507936378 2216 80-81 -2.511243386243393 2216 82-83 -5.7592592592592595 2216 84-85 -1.6957671957671927 2216 86-87 -5.396164021164015 2216 88-89 -1.9814814814814845 2216 90-91 -4.532407407407405 2216 92-93 -2.615079365079364 2216 94-95 0.15277777777777857 2216 96-97 -3.9543650793650826 2216 98-99 -0.36111111111111427 2216 100 -2.2539682539682495 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 4.0 11 4.0 12 4.0 13 7.0 14 6.0 15 11.0 16 8.0 17 17.0 18 5.0 19 7.0 20 10.0 21 11.0 22 11.0 23 12.0 24 11.0 25 14.0 26 29.0 27 27.0 28 36.0 29 40.0 30 56.0 31 70.0 32 71.0 33 117.0 34 142.0 35 157.0 36 237.0 37 224.0 38 148.0 39 29.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.06557377049181 16.459016393442624 12.983606557377051 26.491803278688526 2 16.524590163934427 17.704918032786885 38.950819672131146 26.81967213114754 3 19.934426229508198 23.344262295081965 28.852459016393446 27.86885245901639 4 14.098360655737704 14.22950819672131 34.622950819672134 37.049180327868854 5 15.016393442622949 33.63934426229508 35.868852459016395 15.475409836065573 6 32.721311475409834 34.622950819672134 16.0 16.65573770491803 7 31.868852459016395 28.78688524590164 19.01639344262295 20.327868852459016 8 28.41207349081365 34.90813648293963 18.241469816272964 18.438320209973753 9 29.18032786885246 15.540983606557376 18.950819672131146 36.32786885245902 10-11 25.639344262295083 25.278688524590166 25.573770491803277 23.508196721311474 12-13 27.57377049180328 22.098360655737707 27.409836065573774 22.918032786885245 14-15 25.114754098360653 22.885245901639344 24.950819672131146 27.049180327868854 16-17 23.573770491803277 26.852459016393443 24.852459016393443 24.721311475409834 18-19 25.08196721311475 26.065573770491802 26.32786885245902 22.524590163934427 20-21 25.47540983606557 23.114754098360656 28.819672131147538 22.59016393442623 22-23 25.37704918032787 22.098360655737707 27.147540983606554 25.37704918032787 24-25 25.37704918032787 23.344262295081965 27.311475409836067 23.9672131147541 26-27 23.80327868852459 24.0327868852459 26.852459016393443 25.311475409836063 28-29 24.16393442622951 25.540983606557376 26.983606557377048 23.311475409836067 30-31 23.311475409836067 24.885245901639344 26.885245901639344 24.91803278688525 32-33 23.18032786885246 24.19672131147541 26.688524590163937 25.934426229508194 34-35 23.4251968503937 24.114173228346456 27.559055118110237 24.901574803149607 36-37 25.03280839895013 23.982939632545932 26.837270341207347 24.146981627296586 38-39 26.156875615359372 24.187725631768952 25.402034788316374 24.253363964555298 40-41 24.852265265922522 23.93302692055154 26.887721602101117 24.32698621142482 42-43 23.637557452396585 22.390019697964544 28.95600787918582 25.016414970453056 44-45 23.908045977011493 25.8128078817734 25.97701149425287 24.302134646962234 46-47 24.794745484400657 24.23645320197044 25.12315270935961 25.845648604269293 48-49 23.160315374507228 24.704336399474375 27.201051248357423 24.93429697766097 50-51 23.678160919540232 23.316912972085387 26.108374384236456 26.89655172413793 52-53 23.013788575180566 24.261326329612608 26.723571897570586 26.001313197636243 54-55 24.334975369458128 24.400656814449917 26.272577996715928 24.991789819376027 56-57 26.526592252133945 24.359816152330925 24.458305975049242 24.655285620485884 58-59 24.983585029546948 23.736047275114906 25.344714379514116 25.93565331582403 60-61 23.539067629678268 25.968483256730142 24.983585029546948 25.508864084044646 62-63 23.40774786605384 24.162836506894287 27.64281024294156 24.78660538411031 64-65 23.54098360655738 24.59016393442623 26.295081967213115 25.573770491803277 66-67 25.442622950819672 23.442622950819672 26.163934426229506 24.950819672131146 68-69 24.360655737704917 23.80327868852459 26.491803278688526 25.344262295081965 70-71 24.852459016393443 23.37704918032787 25.967213114754102 25.80327868852459 72-73 25.049180327868854 25.049180327868854 23.901639344262296 26.0 74-75 23.9672131147541 25.37704918032787 24.786885245901637 25.868852459016395 76-77 24.75409836065574 24.65573770491803 25.540983606557376 25.049180327868854 78-79 25.540983606557376 25.278688524590166 24.81967213114754 24.360655737704917 80-81 24.688524590163937 25.114754098360653 25.114754098360653 25.08196721311475 82-83 25.18032786885246 24.19672131147541 25.60655737704918 25.016393442622952 84-85 24.950819672131146 24.39344262295082 24.688524590163937 25.967213114754102 86-87 25.245901639344265 24.65573770491803 24.098360655737704 26.0 88-89 24.42622950819672 25.901639344262296 24.065573770491802 25.60655737704918 90-91 23.21311475409836 25.278688524590166 26.0327868852459 25.47540983606557 92-93 22.885245901639344 24.557377049180328 26.32786885245902 26.229508196721312 94-95 24.16393442622951 24.557377049180328 23.86885245901639 27.409836065573774 96-97 24.91803278688525 23.9672131147541 25.967213114754102 25.147540983606557 98-99 23.80327868852459 23.901639344262296 27.278688524590166 25.016393442622952 100 26.491803278688526 22.360655737704917 24.262295081967213 26.885245901639344 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 0.5 6 0.0 7 0.5 8 0.5 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 2.0 16 2.0 17 1.0 18 2.0 19 1.5 20 0.5 21 0.0 22 0.5 23 2.0 24 3.5 25 5.0 26 4.5 27 3.0 28 5.0 29 12.5 30 14.5 31 15.5 32 19.5 33 20.5 34 23.0 35 24.5 36 24.5 37 29.5 38 34.0 39 33.5 40 27.5 41 27.5 42 33.5 43 31.0 44 37.0 45 46.5 46 47.5 47 51.0 48 60.5 49 60.5 50 46.0 51 42.0 52 50.5 53 52.5 54 49.0 55 39.0 56 34.5 57 40.5 58 42.0 59 49.5 60 55.0 61 53.5 62 51.0 63 45.0 64 38.0 65 32.5 66 26.5 67 20.5 68 18.5 69 11.0 70 5.0 71 6.0 72 4.0 73 1.0 74 1.0 75 0.5 76 0.5 77 0.5 78 0.5 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.06557377049180328 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.06557377049180328 36-37 0.06557377049180328 38-39 0.09836065573770492 40-41 0.13114754098360656 42-43 0.13114754098360656 44-45 0.16393442622950818 46-47 0.16393442622950818 48-49 0.19672131147540983 50-51 0.16393442622950818 52-53 0.13114754098360656 54-55 0.16393442622950818 56-57 0.13114754098360656 58-59 0.13114754098360656 60-61 0.13114754098360656 62-63 0.13114754098360656 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1525.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.09836065573771 #Duplication Level Percentage of deduplicated Percentage of total 1 95.0509461426492 85.63934426229508 2 2.8384279475982535 5.114754098360655 3 0.8005822416302766 2.1639344262295084 4 0.5094614264919942 1.8360655737704918 5 0.21834061135371177 0.9836065573770493 6 0.0727802037845706 0.39344262295081966 7 0.21834061135371177 1.377049180327869 8 0.1455604075691412 1.0491803278688525 9 0.0 0.0 >10 0.1455604075691412 1.4426229508196722 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCG 11 0.7213114754098361 No Hit TGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACT 11 0.7213114754098361 No Hit ATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAA 8 0.5245901639344263 No Hit CCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCCAAGTCCC 8 0.5245901639344263 No Hit GTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGCCCCTCTA 7 0.45901639344262296 No Hit CGCTACGAGAGTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGC 7 0.45901639344262296 No Hit CAGTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTGTCTACTGTGGGCA 7 0.45901639344262296 No Hit CTTCAGGGGAGATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTG 6 0.39344262295081966 No Hit GTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATC 5 0.32786885245901637 No Hit CTACGAGAGTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCA 5 0.32786885245901637 No Hit GTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGC 5 0.32786885245901637 No Hit GGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGC 4 0.26229508196721313 No Hit ACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGG 4 0.26229508196721313 No Hit CCATGAAGGCCTCGGGCACGCTACGAGAGTACAAGGTAGTGGGTCGCTGC 4 0.26229508196721313 No Hit CCCCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCCAAGTC 4 0.26229508196721313 No Hit GTCCCGCTTCTGGTACTTTGTATCTCAGTTAAAGAAGATGAAGAAGTCTT 4 0.26229508196721313 No Hit TTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTGTCTAC 4 0.26229508196721313 No Hit GGGGTAGGAAATCCTCCAGGATCTTAGGAAATTTCACTCCGCTTCTCCTG 4 0.26229508196721313 No Hit TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG 3 0.19672131147540983 RNA PCR Primer, Index 46 (96% over 28bp) CTTCTGGTACTTTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGG 3 0.19672131147540983 No Hit GGCCTCGGGCACGCTACGAGAGTACAAGGTAGTGGGTCGCTGCCTGCCCA 3 0.19672131147540983 No Hit CCCACAACATGTACCGGGAATACCGGGACCTGACCACCGCAGGCGCTGTC 3 0.19672131147540983 No Hit TATCAACGCAGAGTACATGGGGCCTTTTGCGGGTGGCGGCGAACGCGGAG 3 0.19672131147540983 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3 0.19672131147540983 No Hit GTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTATGACTCCC 3 0.19672131147540983 No Hit CCCCCAAATGCCACACGCCGCCCCTCTACCGCATGCGAATCTTTGCGCCT 3 0.19672131147540983 No Hit GAGATACGGAGGCCCGGTCAGACTCTCCCTCTGCACCTCAATGGAAACGA 3 0.19672131147540983 No Hit TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG 3 0.19672131147540983 RNA PCR Primer, Index 27 (96% over 30bp) GAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTATGACT 3 0.19672131147540983 No Hit TCACAGCGCTGCGCCGCACAGTGCCCCCCGCTGTCACTGGGATCACCTTC 2 0.13114754098360656 No Hit CCCTCAGCCCTCGCCATCATGGAAAATGCCAATGTTCTGGCCCGTTATGC 2 0.13114754098360656 No Hit GTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTGTCTACTGTGGGCAGG 2 0.13114754098360656 No Hit GCCTCAGAGGCTGCTGTCAGCGCATTGATCCGCACCATAGCTGGCTCCCA 2 0.13114754098360656 No Hit GCCTGAGATCCTCCCTGATGGGGACCATGACTTGAAGCGCTGCCAGTATG 2 0.13114754098360656 No Hit GATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGA 2 0.13114754098360656 No Hit AGTGTACTTGGCGTGCTGGCTGATTTCAATTTTCTTCACCATTTTCCGGA 2 0.13114754098360656 No Hit GGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTG 2 0.13114754098360656 No Hit ATGCCACACGCCGCCCCTCTACCGCATGCGAATCTTTGCGCCTAATCATG 2 0.13114754098360656 No Hit GTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTT 2 0.13114754098360656 No Hit GTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCT 2 0.13114754098360656 No Hit ACATTCCACTCTCCTGACAGATGGAGTTAATATCAGCTCCTGAAATCTTA 2 0.13114754098360656 No Hit CGAGAGTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACAC 2 0.13114754098360656 No Hit ATCATGTCGTCGCCAAGTCCCGCTTCTGGTACTTTGTATCTCAGTTAAAG 2 0.13114754098360656 No Hit GTACATGGGGTCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATG 2 0.13114754098360656 No Hit AGATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAG 2 0.13114754098360656 No Hit CCCCGGAACACGCCTCCGCCTGAGCCTCGGGCCCGTCGGGATCTGCCTTT 2 0.13114754098360656 No Hit GTATCAACGCAGAGTACATGGGGCCTTTTGCGGGTGGCGGCGAACGCGGA 2 0.13114754098360656 No Hit TCTTTGCGCCTAATCATGTCGTCGCCAAGTCCCGCTTCTGGTACTTTGTA 2 0.13114754098360656 No Hit CTACTGGTCTTTCAACTCCTTCAGTCTTCTGATGGCGGACTTTACCGTGA 2 0.13114754098360656 No Hit GTACTGGACTGTGTGCCAGAGGAGAGGCGTAAACTGATTGTGGCATATGT 2 0.13114754098360656 No Hit CTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTATGACTCCCGGAGCGG 2 0.13114754098360656 No Hit GTACCTGGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAA 2 0.13114754098360656 No Hit ACCGGGACCTGACCACCGCAGGCGCTGTCACCCAGTGCTACCGAGACATG 2 0.13114754098360656 No Hit CTGCCTGCCCACCCCCAAATGCCACACGCCGCCCCTCTACCGCATGCGAA 2 0.13114754098360656 No Hit TAATCATGTCGTCGCCAAGTCCCGCTTCTGGTACTTTGTATCTCAGTTAA 2 0.13114754098360656 No Hit GTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTGTCTACTG 2 0.13114754098360656 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2 0.13114754098360656 No Hit GTGGAGGGGGCAGGACCTCCTGCTGGTGCAGCACCAGCTGCTGGAGCAGG 2 0.13114754098360656 No Hit ATAATATCCCGGGGCAAACCGGAGAATTTGGCAGTCCGATTGGGGGGGGG 2 0.13114754098360656 No Hit CTGTAGACCTGGTCTTTTTCCCCCCAGTTTTTTCTTTTTTACATTCTGGG 2 0.13114754098360656 No Hit GTCGTCGCCAAGTCCCGCTTCTGGTACTTTGTATCTCAGTTAAAGAAGAT 2 0.13114754098360656 No Hit TAGTCATCCCACTCGGCCTGGTAACACGTGCCAGCCACCGGGGCCCTGAG 2 0.13114754098360656 No Hit TGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGC 2 0.13114754098360656 No Hit ACATGGGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAAG 2 0.13114754098360656 No Hit GTCTTCAGGGGAGATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCC 2 0.13114754098360656 No Hit TGTCCATCTTGCAGGTGGCTCTTCCTCCACATCACGACTGGGGTCTCGAA 2 0.13114754098360656 No Hit GGCACCCACAACATGTACCGGGAATACCGGGACCTGACCACCGCAGGCGC 2 0.13114754098360656 No Hit GGCAAGAAGGGTGTGCTGTTTGGAGTTCCTGGGGCCTTCACCCCTGGATG 2 0.13114754098360656 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.03278688524590164 0.0 38-39 0.0 0.0 0.0 0.1639344262295082 0.0 40-41 0.0 0.0 0.0 0.22950819672131148 0.0 42-43 0.0 0.0 0.0 0.29508196721311475 0.0 44-45 0.0 0.0 0.0 0.42622950819672134 0.0 46-47 0.0 0.0 0.0 0.4918032786885246 0.0 48-49 0.0 0.0 0.0 0.5573770491803278 0.0 50-51 0.0 0.0 0.0 0.6557377049180327 0.0 52-53 0.0 0.0 0.0 0.8524590163934427 0.0 54-55 0.0 0.0 0.0 1.0163934426229508 0.0 56-57 0.0 0.0 0.0 1.081967213114754 0.0 58-59 0.0 0.0 0.0 1.1147540983606556 0.0 60-61 0.0 0.0 0.0 1.3114754098360657 0.0 62-63 0.0 0.0 0.0 1.4426229508196722 0.0 64-65 0.0 0.0 0.0 1.4426229508196722 0.0 66-67 0.0 0.0 0.0 1.4426229508196722 0.0 68-69 0.0 0.0 0.0 1.7377049180327868 0.0 70-71 0.0 0.0 0.0 1.8688524590163933 0.0 72-73 0.0 0.0 0.0 2.0327868852459017 0.0 74-75 0.0 0.0 0.0 2.2950819672131146 0.0 76-77 0.0 0.0 0.0 2.5245901639344264 0.0 78-79 0.0 0.0 0.0 2.6557377049180326 0.0 80-81 0.0 0.0 0.0 2.7540983606557377 0.0 82-83 0.0 0.0 0.0 2.918032786885246 0.0 84-85 0.0 0.0 0.0 3.180327868852459 0.0 86-87 0.0 0.0 0.0 3.4098360655737707 0.0 88 0.0 0.0 0.0 3.540983606557377 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE