FastQCFastQC Report
Wed 25 May 2016
SRR1032948_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032948_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1540967
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20200.035.367171
GGTATCA12250.033.3803751
CAACGCA21450.032.430725
TCAACGC21450.032.2115944
ATCAACG22000.031.4063013
AACGCAG24050.029.3155696
TATCAAC25400.027.756562
GTACATG53150.027.678921
TACATGG51900.026.2626572
ACATGGG52850.024.9909923
ACGCAGA28700.023.9107467
TACCTGG12450.023.783692
GTACCGT1000.001758376623.5013166
CGCAGAG30650.022.388788
CATGGGG36900.020.6353024
GTACCTG15950.020.0380881
GAGTACA37050.019.9167981
CCCTATA5150.019.1654992
GCAGAGT34850.018.2070279
TATAACG1550.001033378818.1939752
Produced by FastQC (version 0.11.5)