FastQCFastQC Report
Wed 25 May 2016
SRR1032948_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032948_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1540967
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32090.2082458612027383No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26340.17093162929511146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22850.050.58221
GTATCAA42250.042.9249081
ATCAACG50050.035.906433
TCAACGC50150.035.834834
CAACGCA51150.035.221645
AACGCAG52850.034.1776926
TATCAAC53300.033.8924372
ACGCAGA60600.029.4953357
GTACATG54100.028.225061
CGCAGAG63550.027.7560798
TACATGG52800.027.441992
ACATGGG53250.026.5041923
TACCTGG12100.026.437622
GCAGAGT68800.025.4690539
GTACCTG15500.022.7341331
CATGGGG36950.021.6444934
AGAGTAC63100.021.14303410-11
CAGAGTA67350.020.99458110-11
GGTCTAG4350.020.521691
GAGTACT44200.020.45977612-13
Produced by FastQC (version 0.11.5)