##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032948_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1540967 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.83049020517636 34.0 31.0 34.0 31.0 34.0 2 32.86898032209645 34.0 31.0 34.0 31.0 34.0 3 32.95260378710251 34.0 31.0 34.0 31.0 34.0 4 36.371850922180684 37.0 37.0 37.0 35.0 37.0 5 36.35284921740699 37.0 37.0 37.0 35.0 37.0 6 36.41188292805751 37.0 37.0 37.0 35.0 37.0 7 36.37488083781158 37.0 37.0 37.0 35.0 37.0 8 36.30717335283624 37.0 37.0 37.0 35.0 37.0 9 38.14832828996338 39.0 39.0 39.0 37.0 39.0 10-11 38.16228316375367 39.0 39.0 39.0 37.0 39.0 12-13 38.163594677887325 39.0 39.0 39.0 37.0 39.0 14-15 39.72018771329951 41.0 40.0 41.0 37.0 41.0 16-17 39.72765996935691 41.0 40.0 41.0 37.0 41.0 18-19 39.74180595690888 41.0 40.0 41.0 37.0 41.0 20-21 39.75149857200057 41.0 40.0 41.0 37.0 41.0 22-23 39.718100063142174 41.0 40.0 41.0 37.0 41.0 24-25 39.69828912624345 41.0 40.0 41.0 37.0 41.0 26-27 39.626701934564466 41.0 40.0 41.0 37.0 41.0 28-29 39.56542644975525 41.0 40.0 41.0 37.0 41.0 30-31 39.45763504344999 41.0 39.5 41.0 36.0 41.0 32-33 39.336853092895566 41.0 39.0 41.0 35.5 41.0 34-35 39.245986124297275 41.0 39.0 41.0 35.0 41.0 36-37 39.16052842143927 41.0 39.0 41.0 35.0 41.0 38-39 39.062473758360824 41.0 39.0 41.0 35.0 41.0 40-41 38.9603294554653 41.0 39.0 41.0 35.0 41.0 42-43 38.827389879212205 41.0 39.0 41.0 35.0 41.0 44-45 38.65662632619647 41.0 39.0 41.0 35.0 41.0 46-47 38.44041338977408 41.0 38.0 41.0 35.0 41.0 48-49 38.238518410842026 41.0 38.0 41.0 34.0 41.0 50-51 38.07873238038193 40.0 38.0 41.0 34.0 41.0 52-53 37.92717754500907 40.0 37.5 41.0 34.0 41.0 54-55 37.76837109425445 40.0 37.0 41.0 34.0 41.0 56-57 37.549393011011915 40.0 37.0 41.0 33.0 41.0 58-59 37.366365730090266 40.0 36.0 41.0 33.0 41.0 60-61 37.1635168047077 39.5 36.0 41.0 33.0 41.0 62-63 36.91309450494397 39.0 35.0 41.0 33.0 41.0 64-65 36.62142245745691 39.0 35.0 41.0 32.0 41.0 66-67 36.33456751507333 38.0 35.0 41.0 32.0 41.0 68-69 36.03693362674217 37.0 35.0 40.0 32.0 41.0 70-71 35.70798109239199 37.0 35.0 39.5 31.5 41.0 72-73 35.36258368933274 36.0 35.0 39.0 31.0 41.0 74-75 35.02373022913534 36.0 35.0 39.0 31.0 41.0 76-77 34.68478364559397 35.5 35.0 38.0 31.0 40.0 78-79 34.352413452072625 35.0 35.0 37.0 31.0 39.0 80-81 34.0388768870456 35.0 34.0 37.0 30.5 39.0 82-83 33.75011891883473 35.0 34.0 36.0 30.0 38.5 84-85 33.490819076592814 35.0 34.0 36.0 30.0 37.0 86-87 33.36147659229562 35.0 34.0 36.0 30.0 37.0 88-89 33.18880839109468 35.0 34.0 35.0 30.0 36.5 90-91 33.03327196494149 35.0 34.0 35.0 30.0 36.0 92-93 32.883821327776644 35.0 34.0 35.0 30.0 36.0 94-95 32.79294689633198 35.0 34.0 35.0 30.0 36.0 96-97 32.7383457919605 35.0 34.0 35.0 30.0 36.0 98-99 32.637285224148215 35.0 34.0 35.0 30.0 35.5 100 32.56114180251751 35.0 34.0 35.0 30.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1784705300435192 1101 2 0.22483030751360644 1101 3 0.17766034762330918 1101 4 0.11056845652392866 1101 5 0.16753666884753216 1101 6 0.13974436755462705 1101 7 0.07530969489806694 1101 8 -5.299375892704461E-4 1101 9 0.12227467912262568 1101 10-11 0.15387880734058257 1101 12-13 0.22212816603133945 1101 14-15 0.2663805807996056 1101 16-17 0.2510770930342403 1101 18-19 0.2344856158594908 1101 20-21 0.22808655523155608 1101 22-23 0.22112255097133016 1101 24-25 0.24741439539566557 1101 26-27 0.3372097205982101 1101 28-29 0.2889916503292014 1101 30-31 0.2469950343432643 1101 32-33 0.30961338296518903 1101 34-35 0.301375195310186 1101 36-37 0.2905600246020015 1101 38-39 0.30098989567240864 1101 40-41 0.3007687322050643 1101 42-43 0.2851806255968583 1101 44-45 0.22865616879546735 1101 46-47 0.2532621216314226 1101 48-49 0.2628508581501521 1101 50-51 0.250454572610721 1101 52-53 0.2633564808860882 1101 54-55 0.20340302206975025 1101 56-57 0.2204937049795106 1101 58-59 0.22821861761536866 1101 60-61 0.2652540893067581 1101 62-63 0.2583207202916853 1101 64-65 0.2970498833718125 1101 66-67 0.27605351420151436 1101 68-69 0.2644145867422907 1101 70-71 0.30558886839470034 1101 72-73 0.3408707035107241 1101 74-75 0.32763288413813996 1101 76-77 0.22536653588086608 1101 78-79 0.16441948897677605 1101 80-81 0.23436320933269172 1101 82-83 0.18239800760463964 1101 84-85 0.1444876090782259 1101 86-87 0.18004795156939224 1101 88-89 0.1273257354652486 1101 90-91 0.11982008510459963 1101 92-93 0.21087422902874664 1101 94-95 0.19799534180393152 1101 96-97 0.23094019732364046 1101 98-99 0.236226621008349 1101 100 0.21574840033011355 1102 1 0.17627094174852687 1102 2 0.21429259015030055 1102 3 0.19360902722534945 1102 4 0.16008041506880488 1102 5 0.16070300657463576 1102 6 0.13310627972339262 1102 7 0.22038515126270397 1102 8 -0.024470954002126177 1102 9 0.07740802968315563 1102 10-11 0.12809512598877149 1102 12-13 0.16995337824000956 1102 14-15 0.23658783145154416 1102 16-17 0.21284322594912908 1102 18-19 0.2962920287870503 1102 20-21 0.2549500571708734 1102 22-23 0.25673884140686454 1102 24-25 0.20592962336264264 1102 26-27 0.17899873572277158 1102 28-29 0.20786144227544412 1102 30-31 0.1960757720500581 1102 32-33 0.22240434676020726 1102 34-35 0.19104270627587994 1102 36-37 0.21694029122406278 1102 38-39 0.20759207270722868 1102 40-41 0.13689024967963803 1102 42-43 0.1040897196835644 1102 44-45 0.177794018400391 1102 46-47 0.14119734238295223 1102 48-49 0.13093329652235042 1102 50-51 0.19433708729856392 1102 52-53 0.22944075183242774 1102 54-55 0.20669949170141422 1102 56-57 0.21528715964389278 1102 58-59 0.22253665994017524 1102 60-61 0.2531555214526193 1102 62-63 0.2381079977165257 1102 64-65 0.2511034133448362 1102 66-67 0.28645651406377937 1102 68-69 0.17981438875438016 1102 70-71 0.19940671868204163 1102 72-73 0.20047873487919077 1102 74-75 0.23963187547336418 1102 76-77 0.25760292546016217 1102 78-79 0.20759587590125506 1102 80-81 0.2361866289143535 1102 82-83 0.18448950981192525 1102 84-85 0.12155182448973534 1102 86-87 0.1841421152535503 1102 88-89 0.11303777415403005 1102 90-91 0.162063644359705 1102 92-93 0.23571135088868544 1102 94-95 0.16377179106255824 1102 96-97 0.15631991342445417 1102 98-99 0.2524258023420529 1102 100 0.2470565395142046 1103 1 0.1784345226880859 1103 2 0.20887242701414976 1103 3 0.23353983141854684 1103 4 0.13250935231243233 1103 5 0.13233244299118496 1103 6 0.1287801278251024 1103 7 0.11675788590094527 1103 8 0.06411547480463042 1103 9 0.11158194313063063 1103 10-11 0.16525868369877372 1103 12-13 0.18017701021591392 1103 14-15 0.2492873304404597 1103 16-17 0.2753689173365572 1103 18-19 0.28773314821747675 1103 20-21 0.2944850498757745 1103 22-23 0.253890796457064 1103 24-25 0.17161239363503 1103 26-27 0.1577567178537862 1103 28-29 0.21612631742464572 1103 30-31 0.2596779010088497 1103 32-33 0.24705919086057548 1103 34-35 0.15969825413088756 1103 36-37 0.17173233718061454 1103 38-39 0.15735572709259316 1103 40-41 0.2290180752777644 1103 42-43 0.3205895139504733 1103 44-45 0.24510025206268438 1103 46-47 0.25894817970756634 1103 48-49 0.30582905135072025 1103 50-51 0.27159658106789664 1103 52-53 0.3117497457723388 1103 54-55 0.2505387197205451 1103 56-57 0.23290910321464509 1103 58-59 0.21512239064817607 1103 60-61 0.26128314020666465 1103 62-63 0.3079356485273692 1103 64-65 0.36052857467413446 1103 66-67 0.2620651739626325 1103 68-69 0.21365632150218516 1103 70-71 0.26829124939142446 1103 72-73 0.2994184772007671 1103 74-75 0.3150923913836863 1103 76-77 0.31707695976193406 1103 78-79 0.32049254334829413 1103 80-81 0.3356334826119678 1103 82-83 0.38146829354856493 1103 84-85 0.29558202574457937 1103 86-87 0.22899395005542544 1103 88-89 0.2154804329972535 1103 90-91 0.24740480275519872 1103 92-93 0.34407350739167697 1103 94-95 0.37183875036780734 1103 96-97 0.3589659016088902 1103 98-99 0.30852256173775316 1103 100 0.34028625420067726 1104 1 0.11390186728169738 1104 2 0.1452908313365171 1104 3 0.19044919709152452 1104 4 0.10129496017648876 1104 5 0.04737349519782441 1104 6 0.09845696694556949 1104 7 0.0896985714887748 1104 8 -0.0814512644371348 1104 9 -0.005125012558544029 1104 10-11 0.0956092417590142 1104 12-13 0.15763000178475295 1104 14-15 0.17661403359151961 1104 16-17 0.18129183928944315 1104 18-19 0.1879728493461883 1104 20-21 0.15284230330357218 1104 22-23 0.19676825554765287 1104 24-25 0.178934411190518 1104 26-27 0.15432161480457296 1104 28-29 0.10700247539117669 1104 30-31 0.13481045786284795 1104 32-33 0.1250132700877984 1104 34-35 0.07443977673646884 1104 36-37 0.0675357280415696 1104 38-39 0.12438762496209677 1104 40-41 0.14501009040333201 1104 42-43 0.17777798235125886 1104 44-45 0.19389840648017298 1104 46-47 0.164789975026423 1104 48-49 0.18365011387728458 1104 50-51 0.14830142630286502 1104 52-53 0.15451674485167644 1104 54-55 0.06585393735387157 1104 56-57 0.21896597124252537 1104 58-59 0.11893844036195844 1104 60-61 0.07607635925922551 1104 62-63 -0.012309319055866297 1104 64-65 0.07983787529090591 1104 66-67 0.0636329122817969 1104 68-69 0.038782343692929544 1104 70-71 0.06461237920080976 1104 72-73 -0.013613480690828794 1104 74-75 -0.07988463080415897 1104 76-77 -0.010218861054696049 1104 78-79 -0.03193772211393053 1104 80-81 -0.04678207743771168 1104 82-83 -0.059926329440088466 1104 84-85 0.04664514284671384 1104 86-87 -0.02470646032142554 1104 88-89 0.009969340043362251 1104 90-91 -0.016481066204953265 1104 92-93 -0.03230257167484751 1104 94-95 -0.018915427218203718 1104 96-97 -0.04505131892292269 1104 98-99 -0.005796676407641144 1104 100 0.021986970496577385 1105 1 0.014176109106756485 1105 2 0.052411255300555126 1105 3 0.049015820340407856 1105 4 0.00972584524473774 1105 5 0.028801377894225766 1105 6 0.007739799222953536 1105 7 -0.024737241635932605 1105 8 -0.07473044998961598 1105 9 -0.02865228786691887 1105 10-11 0.030110891358162917 1105 12-13 0.02001407123844956 1105 14-15 0.09806315504977192 1105 16-17 0.11195253624162405 1105 18-19 0.12828224943412891 1105 20-21 0.11834166770780996 1105 22-23 0.1474809616553614 1105 24-25 0.10870186334108212 1105 26-27 0.13687052433610347 1105 28-29 0.07627900241113394 1105 30-31 0.0719879702175561 1105 32-33 0.14161812863893175 1105 34-35 0.14725075083421757 1105 36-37 0.15133674739152525 1105 38-39 0.19864401479662774 1105 40-41 0.24546224373170844 1105 42-43 0.13643319262329356 1105 44-45 0.20602938990674602 1105 46-47 0.31985229475275645 1105 48-49 0.3216382758486489 1105 50-51 0.2805291272161128 1105 52-53 0.2714911615462299 1105 54-55 0.2579108800967873 1105 56-57 0.2368053328259876 1105 58-59 0.2528712340611179 1105 60-61 0.2646954617924919 1105 62-63 0.2791309252636509 1105 64-65 0.3085729798116077 1105 66-67 0.31513349902103727 1105 68-69 0.24448104272658355 1105 70-71 0.2305808584446254 1105 72-73 0.2616924948311308 1105 74-75 0.2747613022587174 1105 76-77 0.3068679256515452 1105 78-79 0.28264871660477553 1105 80-81 0.2722292876966037 1105 82-83 0.28553512967391015 1105 84-85 0.21162192677826397 1105 86-87 0.2986673317470405 1105 88-89 0.34081366379067646 1105 90-91 0.25407955783116165 1105 92-93 0.2788092461658991 1105 94-95 0.2145783030144912 1105 96-97 0.16182555986403457 1105 98-99 0.17449266484967296 1105 100 0.22896866036087715 1106 1 0.038649354036522254 1106 2 0.09108447182495638 1106 3 0.04169836726799048 1106 4 -0.014597783645328377 1106 5 0.06289937929136613 1106 6 0.0474731574200149 1106 7 0.01602029978946007 1106 8 -0.04779179292728486 1106 9 0.02242659194983787 1106 10-11 0.03674956103304794 1106 12-13 0.015658853126090833 1106 14-15 -0.003266837581463733 1106 16-17 0.08557674628519862 1106 18-19 0.05514521306767506 1106 20-21 0.026734820352672273 1106 22-23 0.05700978677553792 1106 24-25 0.15223823359090716 1106 26-27 0.06262826271981226 1106 28-29 0.02139403871471046 1106 30-31 0.07962001450390233 1106 32-33 0.058832122581293333 1106 34-35 0.12639358825542502 1106 36-37 0.14246847347273928 1106 38-39 0.12201656993862997 1106 40-41 0.15851093074253697 1106 42-43 0.11936910383376897 1106 44-45 0.1757974633617181 1106 46-47 0.17677463860727016 1106 48-49 0.1284818905866274 1106 50-51 0.21093065474528316 1106 52-53 0.17091991015983865 1106 54-55 0.1424234697083051 1106 56-57 0.09254448123606807 1106 58-59 0.088596617072767 1106 60-61 0.10520458276933908 1106 62-63 0.1445899664272119 1106 64-65 0.14290547001880327 1106 66-67 0.13305041730645684 1106 68-69 0.15388979555402216 1106 70-71 0.20263274485352412 1106 72-73 0.12741363510772175 1106 74-75 0.20111913106255486 1106 76-77 0.24880669293824553 1106 78-79 0.15745342972686416 1106 80-81 0.13720017942627294 1106 82-83 0.19950970145220737 1106 84-85 0.2440249121603415 1106 86-87 0.2441976971173574 1106 88-89 0.26711231475271546 1106 90-91 0.2274618163322657 1106 92-93 0.2501977986596273 1106 94-95 0.28098270794199465 1106 96-97 0.20972654440262772 1106 98-99 0.2465944955500845 1106 100 0.2939573144778649 1107 1 0.00313981367312266 1107 2 0.03741469760812066 1107 3 0.04999060463319438 1107 4 0.054260833459387925 1107 5 0.016376899548589563 1107 6 0.05618947397314855 1107 7 0.022188595402440114 1107 8 0.010549234819407616 1107 9 0.016754000939471325 1107 10-11 0.06254552504730526 1107 12-13 0.04619946899119043 1107 14-15 0.009696653313596926 1107 16-17 0.07568605659627536 1107 18-19 0.015983131136714235 1107 20-21 0.0894605999704936 1107 22-23 0.13259995182602324 1107 24-25 0.12331397640319608 1107 26-27 0.055130464669446155 1107 28-29 0.03233730287632852 1107 30-31 0.09866102820917888 1107 32-33 0.18994474893632685 1107 34-35 0.22895515419973833 1107 36-37 0.20000974318568865 1107 38-39 0.2522402610728207 1107 40-41 0.24781931290171144 1107 42-43 0.17724598471006203 1107 44-45 0.11367394001537434 1107 46-47 0.10674235752929206 1107 48-49 0.14948161117658998 1107 50-51 0.18148959536993914 1107 52-53 0.21714666913109681 1107 54-55 0.16179087546414905 1107 56-57 0.21846834271109827 1107 58-59 0.2113533587262708 1107 60-61 0.18004445387196455 1107 62-63 0.24807964882167965 1107 64-65 0.19514840924215804 1107 66-67 0.20458931416160908 1107 68-69 0.19311800689012415 1107 70-71 0.2161974282762884 1107 72-73 0.1965752418998008 1107 74-75 0.18022223903821555 1107 76-77 0.18248882785783138 1107 78-79 0.2618758381278852 1107 80-81 0.16555019226211698 1107 82-83 0.1687749523468156 1107 84-85 0.18248751163810795 1107 86-87 0.12711709228682366 1107 88-89 0.203014660236839 1107 90-91 0.21412761072902953 1107 92-93 0.17894345326627814 1107 94-95 0.21958412598337418 1107 96-97 0.1825078230793835 1107 98-99 0.19682369687706824 1107 100 0.10932756113877673 1108 1 0.11201343012137244 1108 2 0.1077377179385337 1108 3 0.12966594210486448 1108 4 0.00883353356120864 1108 5 0.008282916561761056 1108 6 0.028557441260566918 1108 7 0.06586900726567535 1108 8 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2214 46-47 -0.12665028441345783 2214 48-49 -0.10397363640368695 2214 50-51 -0.10793641856095348 2214 52-53 -0.16345827868489948 2214 54-55 -0.0497201108399139 2214 56-57 -0.050166498159917694 2214 58-59 0.023587606890174584 2214 60-61 -0.016139141328778805 2214 62-63 -0.009594468370615061 2214 64-65 -0.01824605175059446 2214 66-67 -0.010366498082113651 2214 68-69 -0.10174715602853723 2214 70-71 -0.06658621088482874 2214 72-73 -0.08672379476163172 2214 74-75 -0.11259933489479579 2214 76-77 -0.13748301476402958 2214 78-79 -0.09394455342262376 2214 80-81 -0.025551431997953955 2214 82-83 -0.07037990944328243 2214 84-85 -0.06499992554782352 2214 86-87 -0.0853744756584831 2214 88-89 -0.057604991566670094 2214 90-91 -0.07679627718717086 2214 92-93 -0.08571790025851556 2214 94-95 -0.056016160517003755 2214 96-97 -0.0745220472375081 2214 98-99 -0.0405367478806582 2214 100 -0.08024208194845528 2215 1 -0.18841734825058154 2215 2 -0.2648219119740389 2215 3 -0.23253803039322207 2215 4 -0.17433215695742632 2215 5 -0.15530145658143368 2215 6 -0.1629850848407841 2215 7 -0.1952558972415801 2215 8 -0.06719145487812739 2215 9 -0.13409062197136734 2215 10-11 -0.1644199299332243 2215 12-13 -0.1934359694692489 2215 14-15 -0.26549071720336315 2215 16-17 -0.20734291561388574 2215 18-19 -0.1782225547922991 2215 20-21 -0.2146410772467675 2215 22-23 -0.2553486721187568 2215 24-25 -0.280817068431368 2215 26-27 -0.26251150760599273 2215 28-29 -0.2602078330400772 2215 30-31 -0.16448433957703656 2215 32-33 -0.2978726484657628 2215 34-35 -0.31546224729717665 2215 36-37 -0.2941597610710218 2215 38-39 -0.37393764270632346 2215 40-41 -0.35017575538599033 2215 42-43 -0.3491218488555248 2215 44-45 -0.3817163357843043 2215 46-47 -0.33899920999767374 2215 48-49 -0.24276866490460236 2215 50-51 -0.24028201414184025 2215 52-53 -0.3100004619917911 2215 54-55 -0.2594858353115228 2215 56-57 -0.20260474618640245 2215 58-59 -0.2502464022379485 2215 60-61 -0.14961840716098607 2215 62-63 -0.1559456661924017 2215 64-65 -0.11196360870343369 2215 66-67 -0.15595865102721262 2215 68-69 -0.17244116789902364 2215 70-71 -0.23311575976731547 2215 72-73 -0.19584506564761028 2215 74-75 -0.12258444782108313 2215 76-77 -0.20851492403122762 2215 78-79 -0.17780921816964934 2215 80-81 -0.2503805244741031 2215 82-83 -0.198327941803079 2215 84-85 -0.11061362557328636 2215 86-87 -0.24523422375352055 2215 88-89 -0.2747971131705569 2215 90-91 -0.2944217889303289 2215 92-93 -0.2623745769151924 2215 94-95 -0.31252190966981175 2215 96-97 -0.29452753194888004 2215 98-99 -0.2505154946241106 2215 100 -0.2352390457689495 2216 1 -0.28074631745581513 2216 2 -0.2868813940740864 2216 3 -0.34077016337180766 2216 4 -0.20846982456365026 2216 5 -0.19428356980289863 2216 6 -0.1919885357509301 2216 7 -0.24186425845440596 2216 8 -0.2394629967237094 2216 9 -0.3061777640382246 2216 10-11 -0.32750572851597326 2216 12-13 -0.3547594330239576 2216 14-15 -0.4399566164157065 2216 16-17 -0.38892401200228477 2216 18-19 -0.38086605733438006 2216 20-21 -0.3698358824415635 2216 22-23 -0.5495519333605614 2216 24-25 -0.4797765268228744 2216 26-27 -0.519282930908858 2216 28-29 -0.4822001409350207 2216 30-31 -0.5258815630432352 2216 32-33 -0.623396212123204 2216 34-35 -0.694273395580943 2216 36-37 -0.6155580382502279 2216 38-39 -0.6032940874785879 2216 40-41 -0.5150317918444642 2216 42-43 -0.664885395653549 2216 44-45 -0.650589653136322 2216 46-47 -0.6842008643271242 2216 48-49 -0.6857219590749324 2216 50-51 -0.6442018265809182 2216 52-53 -0.6085134094905413 2216 54-55 -0.6103353914349157 2216 56-57 -0.7033950309523505 2216 58-59 -0.708130809919524 2216 60-61 -0.6255581874618201 2216 62-63 -0.7273676519145837 2216 64-65 -0.7612233753060025 2216 66-67 -0.7167078409853218 2216 68-69 -0.7055699052873976 2216 70-71 -0.7472592227951651 2216 72-73 -0.7859072347158644 2216 74-75 -0.8578430026536807 2216 76-77 -0.8209671776599521 2216 78-79 -0.7922477208434415 2216 80-81 -0.8530622241132022 2216 82-83 -0.8585436895441845 2216 84-85 -0.8403717984349015 2216 86-87 -0.745747908851385 2216 88-89 -0.7360428653899405 2216 90-91 -0.8245000271221627 2216 92-93 -0.786500653678182 2216 94-95 -0.8270815893410557 2216 96-97 -0.6777102721295876 2216 98-99 -0.6256215244968715 2216 100 -0.6073419935311257 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 4.0 5 8.0 6 29.0 7 91.0 8 161.0 9 331.0 10 579.0 11 942.0 12 1471.0 13 2392.0 14 2661.0 15 3389.0 16 4265.0 17 6421.0 18 7869.0 19 4379.0 20 2992.0 21 2975.0 22 2933.0 23 3130.0 24 3505.0 25 4166.0 26 5427.0 27 6903.0 28 8806.0 29 11304.0 30 14571.0 31 18892.0 32 24101.0 33 31684.0 34 45583.0 35 70183.0 36 137833.0 37 315474.0 38 539541.0 39 255020.0 40 950.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.358920779460135 18.93165576872659 12.914513252743088 23.794910199070195 2 16.222214579084092 19.588101191399392 39.7117772610728 24.47790696844372 3 18.862908071949676 24.78633532472488 29.449968396447424 26.900788206878023 4 12.667310064986692 16.046384389175074 37.66995483447637 33.61635071136186 5 12.875082497220891 37.56214466167541 35.10445754305268 14.458315298051023 6 31.809945824150436 37.102864025626445 17.201805688594 13.885384461629116 7 28.491404037493606 32.01843530899 21.547857304167024 17.942303349349366 8 26.368579220056727 35.65084527216906 19.490003093568756 18.49057241420546 9 25.83424132240415 16.01290371984573 20.469372484913535 37.68348247283658 10-11 24.815132316266343 26.219867135376685 27.961435903559256 21.003564644797716 12-13 25.533205188440483 23.41537505094214 28.502969569697928 22.54845019091945 14-15 22.46106253583389 25.27105588740039 25.90491215079906 26.36296942596666 16-17 21.916108521467365 28.82180474987459 26.430643874917504 22.83144285374054 18-19 21.7902206180342 27.342158314278738 29.161535958114598 21.706085109572467 20-21 22.710706978150732 26.62500235241897 28.955487041578436 21.70880362785186 22-23 22.492698566384885 26.3663439358921 29.044666505944818 22.0962909917782 24-25 22.14661639497456 26.536769286678435 29.02294673450317 22.29366758384384 26-27 21.991895534951155 26.537060989214602 29.098001854785483 22.373041621048763 28-29 22.11375745246856 26.575649715075816 29.148485396195667 22.162107436259955 30-31 22.211985484962813 26.941444636919627 28.719056405756426 22.127513472361134 32-33 22.03254298937399 26.653493005292074 29.146709564902455 22.16725444043148 34-35 22.11714066961172 26.41288059877336 29.1460287384469 22.32394999316802 36-37 22.441544884160955 26.77192856032255 28.59174401117669 22.1947825443398 38-39 22.294064256600503 26.962847879127054 28.67281670261293 22.07027116165951 40-41 22.107354638469044 26.711815763116586 28.834072200677564 22.34675739773681 42-43 22.11945728510067 26.746184928829063 28.667066282031467 22.4672915040388 44-45 22.240677082961113 26.93067332124473 28.551362371531535 22.277287224262615 46-47 22.317048607275822 27.112964322610406 28.24530398227501 22.32468308783876 48-49 22.304530920013292 27.050475773727207 28.134923458108812 22.510069848150685 50-51 22.34726291161257 27.109789558696356 27.977569993873036 22.565377535818033 52-53 22.45142721635151 27.09411149406991 27.946575622813373 22.507885666765205 54-55 22.42964512717887 27.45403481642353 27.743429890404776 22.372890165992832 56-57 22.23719956709338 27.126140569924424 27.95771884715876 22.67894101582344 58-59 22.162980977844292 26.79808869037875 28.268369332782495 22.77056099899446 60-61 22.417147981433587 27.17155159338113 27.847606266278174 22.563694158907115 62-63 22.185041256148637 27.146691956796747 27.943883193376926 22.724383593677683 64-65 22.224493524031832 26.869987115229303 28.133836783603016 22.77168257713584 66-67 22.107041946415432 26.966428850474312 27.966937622244746 22.95959158086551 68-69 22.425777864592035 27.121405378640855 27.858151269447628 22.594665487319475 70-71 22.455380290428025 26.951096292133446 27.885217528993156 22.70830588844537 72-73 22.273643757458792 27.114435286414313 27.8686370311629 22.743283924964 74-75 22.31092554220824 27.168232674677657 27.727816364659336 22.793025418454775 76-77 22.318831927126208 27.14132795315664 27.599352483881045 22.940487635836103 78-79 22.342446231779288 27.228699400246338 27.660344225635086 22.768510142339284 80-81 22.223188429309605 27.155754925898584 27.736041597378264 22.88501504741355 82-83 22.152262430445347 27.146894039414924 27.775850773979542 22.924992756160183 84-85 22.141097116291263 27.165896479288655 27.591440958826503 23.10156544559358 86-87 22.30793391626226 27.163172943514212 27.60828681453178 22.920606325691747 88-89 21.945424894497517 27.135926393888482 27.693144334145163 23.225504377468837 90-91 21.985541527549294 27.13095365566382 27.65737703322269 23.226127783564195 92-93 22.143432621425628 27.160813093970717 27.544439205985128 23.15131507861853 94-95 21.919206569694303 26.98694218421115 27.67930526177119 23.414545984323354 96-97 22.00370807086218 27.0107069410492 27.615361303288914 23.370223684799697 98-99 21.871962132843944 27.114387987794668 27.600673863018393 23.412976016342995 100 21.939275792408274 26.908038913227863 27.590986698611975 23.56169859575189 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 32.0 1 34.5 2 159.0 3 300.0 4 203.0 5 104.0 6 264.0 7 619.0 8 1022.0 9 1129.5 10 856.5 11 627.5 12 631.0 13 744.5 14 856.5 15 951.0 16 1066.0 17 1197.0 18 1326.0 19 1492.0 20 1699.5 21 2026.5 22 2543.0 23 3313.0 24 4371.5 25 5721.0 26 7658.0 27 10055.0 28 12986.0 29 16553.0 30 20089.0 31 23458.5 32 26866.0 33 30673.0 34 34349.0 35 37612.0 36 40927.5 37 44484.5 38 47621.5 39 49377.5 40 50959.5 41 53015.0 42 54502.5 43 55204.5 44 54902.0 45 54721.5 46 55369.0 47 54704.0 48 53674.5 49 52858.5 50 51673.5 51 50108.0 52 47132.5 53 44754.0 54 43444.0 55 41611.0 56 38595.5 57 35111.5 58 32167.0 59 30039.0 60 27508.0 61 23847.5 62 20562.0 63 17470.5 64 14334.5 65 11485.0 66 9036.5 67 6933.0 68 5222.5 69 3944.5 70 2846.0 71 2103.5 72 1614.0 73 1156.0 74 848.0 75 585.0 76 350.0 77 232.0 78 159.5 79 88.5 80 53.0 81 26.0 82 9.5 83 5.5 84 3.0 85 2.5 86 3.0 87 1.5 88 0.5 89 0.0 90 1.0 91 2.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.9468294908327042E-4 2 3.8936589816654084E-4 3 6.489431636109015E-5 4 0.012329920108607126 5 3.8936589816654084E-4 6 0.0038287646653043187 7 7.138374799719916E-4 8 0.14867287878325752 9 0.0010383090617774424 10-11 0.0 12-13 1.9468294908327042E-4 14-15 1.6223579090272536E-4 16-17 0.0 18-19 3.2447158180545074E-5 20-21 0.0 22-23 1.6223579090272536E-4 24-25 4.5426021452763107E-4 26-27 0.0057106998397759325 28-29 0.007689976488789182 30-31 0.014276749599439833 32-33 0.017294335310230523 34-35 0.027093377080755137 36-37 0.04013713466933426 38-39 0.04575049303456855 40-41 0.05739902282138424 42-43 0.07482314676433693 44-45 0.11525230585729611 46-47 0.12310451813698801 48-49 0.11956777789530859 50-51 0.12122258296251638 52-53 0.11564167175546264 54-55 0.12294228234608529 56-57 0.10415537775954968 58-59 0.09727658022527413 60-61 0.09867180802703758 62-63 0.09730902738345468 64-65 3.2447158180545074E-5 66-67 6.489431636109015E-5 68-69 9.734147454163521E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 9.734147454163521E-5 78-79 6.489431636109015E-5 80-81 2.9202442362490566E-4 82-83 1.6223579090272536E-4 84-85 0.0 86-87 8.436261126941718E-4 88-89 5.191545308887212E-4 90-91 1.297886327221803E-4 92-93 8.76073270874717E-4 94-95 8.111789545136268E-4 96-97 2.595772654443606E-4 98-99 6.489431636109015E-5 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1540967.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.90674976365227 #Duplication Level Percentage of deduplicated Percentage of total 1 81.38099025298237 55.26318540627499 2 11.616370477464482 15.776599263501174 3 3.1234894372564868 6.36318046815562 4 1.314855376193437 3.5715022002624246 5 0.6960032507865539 2.363165929292552 6 0.4403369980658987 1.7941112603603295 7 0.2800466082040846 1.3311938461832042 8 0.21167748894543978 1.1499464217933 9 0.14081074899059007 0.8605800266162807 >10 0.757701372176337 8.84992703348029 >50 0.029396667907084072 1.3030619113909854 >100 0.007843149734794011 0.8136802454254733 >500 1.9126851699535752E-4 0.08487934640449041 >1k 2.869027754930363E-4 0.4749866408587933 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3209 0.2082458612027383 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2634 0.17093162929511146 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 6.489431636109015E-5 0.0 16-17 0.0 0.0 0.0 6.489431636109015E-5 0.0 18-19 0.0 0.0 0.0 6.489431636109015E-5 0.0 20-21 0.0 0.0 0.0 6.489431636109015E-5 0.0 22-23 0.0 0.0 0.0 6.489431636109015E-5 0.0 24-25 0.0 0.0 0.0 1.297886327221803E-4 0.0 26-27 0.0 0.0 0.0 1.9468294908327045E-4 0.0 28-29 0.0 0.0 0.0 0.0011356505363190777 0.0 30-31 0.0 0.0 0.0 0.004802179410720671 0.0 32-33 0.0 0.0 0.0 0.013076204746759664 0.0 34-35 0.0 0.0 0.0 0.023913555579061718 0.0 36-37 0.0 3.2447158180545074E-5 0.0 0.04435526523280511 0.0 38-39 0.0 6.489431636109015E-5 0.0 0.08150726134952924 0.0 40-41 0.0 6.489431636109015E-5 0.0 0.13030778725306902 0.0 42-43 0.0 1.297886327221803E-4 0.0 0.17991949211112243 0.0 44-45 0.0 1.297886327221803E-4 0.0 0.23423603490535488 0.0 46-47 0.0 1.297886327221803E-4 0.0 0.3033484818299159 0.0 48-49 0.0 1.297886327221803E-4 0.0 0.382292417683182 0.0 50-51 0.0 1.297886327221803E-4 0.0 0.4725279645832779 0.0 52-53 0.0 1.297886327221803E-4 0.0 0.5642885279178593 0.0 54-55 0.0 1.297886327221803E-4 0.0 0.6634470433176052 0.0 56-57 0.0 1.297886327221803E-4 0.0 0.7788940321239846 0.0 58-59 0.0 1.297886327221803E-4 0.0 0.9136795272059688 0.0 60-61 0.0 1.297886327221803E-4 0.0 1.061086966820185 0.0 62-63 0.0 1.297886327221803E-4 0.0 1.2109928376143033 0.0 64-65 0.0 1.297886327221803E-4 0.0 1.3649221560228089 0.0 66-67 0.0 1.297886327221803E-4 0.0 1.544874095292112 0.0 68-69 0.0 1.297886327221803E-4 0.0 1.750589078156768 0.0 70-71 0.0 1.297886327221803E-4 0.0 1.9795686734368743 0.0 72-73 0.0 1.297886327221803E-4 0.0 2.213674919709507 0.0 74-75 0.0 1.297886327221803E-4 0.0 2.445152946169516 0.0 76-77 0.0 1.297886327221803E-4 0.0 2.692692315928894 0.0 78-79 0.0 1.297886327221803E-4 0.0 2.982575227113884 0.0 80-81 0.0 1.297886327221803E-4 0.0 3.3026015482485995 0.0 82-83 0.0 1.297886327221803E-4 0.0 3.6321348867302152 0.0 84-85 0.0 1.297886327221803E-4 0.0 3.945931353494267 0.0 86-87 0.0 1.297886327221803E-4 0.0 4.267352902430747 0.0 88 0.0 1.297886327221803E-4 0.0 4.531570111494925 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2285 0.0 50.5822 1 GTATCAA 4225 0.0 42.924908 1 ATCAACG 5005 0.0 35.90643 3 TCAACGC 5015 0.0 35.83483 4 CAACGCA 5115 0.0 35.22164 5 AACGCAG 5285 0.0 34.177692 6 TATCAAC 5330 0.0 33.892437 2 ACGCAGA 6060 0.0 29.495335 7 GTACATG 5410 0.0 28.22506 1 CGCAGAG 6355 0.0 27.756079 8 TACATGG 5280 0.0 27.44199 2 ACATGGG 5325 0.0 26.504192 3 TACCTGG 1210 0.0 26.43762 2 GCAGAGT 6880 0.0 25.469053 9 GTACCTG 1550 0.0 22.734133 1 CATGGGG 3695 0.0 21.644493 4 AGAGTAC 6310 0.0 21.143034 10-11 CAGAGTA 6735 0.0 20.994581 10-11 GGTCTAG 435 0.0 20.52169 1 GAGTACT 4420 0.0 20.459776 12-13 >>END_MODULE