FastQCFastQC Report
Wed 25 May 2016
SRR1032947_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032947_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3942107
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG125350.030.0343171
TACATGG124650.028.5061862
ACATGGG127700.026.5003243
GTATCAA77800.022.8965051
TCAACGC77200.021.9785754
CAACGCA79600.021.315365
ATCAACG79950.021.2225863
CATGGGG92150.021.0141324
AACGCAG81650.020.6645436
TATCAAC89200.019.706812
TACACCG8800.019.2273045
GAGTACA85050.018.5684011
ACACCGT9150.018.4913646
GGTATCA60050.018.0021511
CATGGGA43600.017.894984
ACGCAGA96850.017.275797
AGTACAT87000.016.8556272
CGCAGAG99950.016.6457168
AGAGTAC74250.015.539510-11
GTATTAG18050.015.3632951
Produced by FastQC (version 0.11.5)