##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1032947_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3942107
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.505150671962987	33.0	31.0	34.0	30.0	34.0
2	31.619897176814327	34.0	31.0	34.0	30.0	34.0
3	31.698414071459755	34.0	31.0	34.0	30.0	34.0
4	35.06345007885377	37.0	35.0	37.0	33.0	37.0
5	35.12669366914698	37.0	35.0	37.0	33.0	37.0
6	35.15803528417671	37.0	36.0	37.0	33.0	37.0
7	35.12012307124083	37.0	36.0	37.0	33.0	37.0
8	35.11870707720516	37.0	36.0	37.0	33.0	37.0
9	36.76040148073099	39.0	38.0	39.0	33.0	39.0
10-11	36.75036611132067	39.0	38.0	39.0	33.0	39.0
12-13	36.69894005921199	39.0	38.0	39.0	33.0	39.0
14-15	38.14748179082912	41.0	39.0	41.0	33.5	41.0
16-17	38.15686991753395	41.0	39.0	41.0	33.5	41.0
18-19	38.15061805780513	41.0	39.0	41.0	33.5	41.0
20-21	38.17680798618606	41.0	39.0	41.0	34.0	41.0
22-23	38.150421335595404	41.0	39.0	41.0	34.0	41.0
24-25	38.163738072051316	41.0	39.0	41.0	34.0	41.0
26-27	38.13044635774726	41.0	39.0	41.0	33.0	41.0
28-29	38.108501367415954	41.0	39.0	41.0	33.0	41.0
30-31	38.058246135886215	41.0	39.0	41.0	33.0	41.0
32-33	38.00398441747015	41.0	38.5	41.0	33.0	41.0
34-35	37.932949689087586	41.0	38.0	41.0	33.0	41.0
36-37	37.84112886839449	40.5	38.0	41.0	33.0	41.0
38-39	37.74421064674297	40.0	38.0	41.0	32.5	41.0
40-41	37.658005604617024	40.0	38.0	41.0	32.0	41.0
42-43	37.535566132527606	40.0	38.0	41.0	32.0	41.0
44-45	37.38896394745247	40.0	38.0	41.0	32.0	41.0
46-47	37.253894047015976	40.0	37.5	41.0	31.0	41.0
48-49	37.106545687369724	40.0	37.0	41.0	31.0	41.0
50-51	36.9562094585459	40.0	37.0	41.0	31.0	41.0
52-53	36.79019290952782	40.0	36.5	41.0	30.5	41.0
54-55	36.60915926432236	40.0	36.0	41.0	30.0	41.0
56-57	36.40902022700044	39.5	35.5	41.0	30.0	41.0
58-59	36.18491469155961	39.0	35.0	41.0	30.0	41.0
60-61	35.92693539267199	39.0	35.0	41.0	29.5	41.0
62-63	35.66255748512153	38.5	35.0	40.5	29.0	41.0
64-65	35.37165759326167	38.0	35.0	40.0	29.0	41.0
66-67	35.05982042090689	37.0	35.0	40.0	29.0	41.0
68-69	34.73468794733375	37.0	34.0	40.0	28.0	41.0
70-71	34.38888087005249	36.0	34.0	39.0	28.0	41.0
72-73	34.08857484081482	36.0	34.0	39.0	28.0	41.0
74-75	33.81242492910518	35.5	34.0	38.5	28.0	40.0
76-77	33.492069469448694	35.0	34.0	37.5	27.5	39.5
78-79	33.13041464881598	35.0	34.0	37.0	27.0	39.0
80-81	32.82173682753918	35.0	33.5	36.5	27.0	39.0
82-83	32.520792180425346	35.0	33.0	36.0	26.0	38.0
84-85	32.372016792035325	35.0	33.0	36.0	26.0	37.0
86-87	32.160399882600856	35.0	33.0	35.5	26.0	37.0
88-89	31.943195225294495	35.0	33.0	35.0	26.0	36.5
90-91	31.757972196087017	35.0	33.0	35.0	25.0	36.0
92-93	31.577335039358395	35.0	33.0	35.0	25.0	36.0
94-95	31.400847313378353	35.0	33.0	35.0	25.0	36.0
96-97	31.231102681890675	35.0	33.0	35.0	24.0	35.0
98-99	31.052761885966056	35.0	33.0	35.0	23.5	35.0
100	30.91979821958156	35.0	33.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3700734115815685
1101	2	0.438811458919357
1101	3	0.3995682047233018
1101	4	0.41915546419348004
1101	5	0.34379952786531476
1101	6	0.41477425594418094
1101	7	0.3833530681704005
1101	8	0.3686240291864493
1101	9	0.42228092626527314
1101	10-11	0.42816930271489184
1101	12-13	0.23108004740440435
1101	14-15	0.34003418416339315
1101	16-17	0.38491580936614156
1101	18-19	0.42421598037908126
1101	20-21	0.37045532405851134
1101	22-23	0.36448263302413864
1101	24-25	0.3707490763535901
1101	26-27	0.40330544380731936
1101	28-29	0.38750953018354295
1101	30-31	0.3886323418370665
1101	32-33	0.44528209756209236
1101	34-35	0.4657998092719353
1101	36-37	0.48390500984613993
1101	38-39	0.4859105728795967
1101	40-41	0.5180534453741004
1101	42-43	0.4929185756590613
1101	44-45	0.4962481413569151
1101	46-47	0.48376186133787513
1101	48-49	0.4581708711971899
1101	50-51	0.5669476088215575
1101	52-53	0.5211274634656675
1101	54-55	0.5207243016411596
1101	56-57	0.5596751340721156
1101	58-59	0.516988401970842
1101	60-61	0.6178672036355408
1101	62-63	0.5579320418937641
1101	64-65	0.550424134057991
1101	66-67	0.6379684686117315
1101	68-69	0.5248908268792647
1101	70-71	0.5150805634162694
1101	72-73	0.49587133568413577
1101	74-75	0.5086913975817993
1101	76-77	0.3972688648702771
1101	78-79	0.4329625354996125
1101	80-81	0.46036491791884515
1101	82-83	0.45402256019590226
1101	84-85	0.3570568846346731
1101	86-87	0.33027831886187187
1101	88-89	0.35643110030060043
1101	90-91	0.357861671789486
1101	92-93	0.4713069511797592
1101	94-95	0.413327420407537
1101	96-97	0.38984197876211724
1101	98-99	0.42865475977949075
1101	100	0.4210288459621019
1102	1	0.3522426535283927
1102	2	0.4115052816574014
1102	3	0.41828520650047096
1102	4	0.3672218390881099
1102	5	0.3996020637501161
1102	6	0.271341224804587
1102	7	0.38864592552879884
1102	8	0.33696216239655996
1102	9	0.41373121160632564
1102	10-11	0.4166507412173388
1102	12-13	0.4210951192972061
1102	14-15	0.5511082132083445
1102	16-17	0.43622966738323754
1102	18-19	0.41595354410340235
1102	20-21	0.4489489597058096
1102	22-23	0.4677404366702831
1102	24-25	0.46368035684309916
1102	26-27	0.44239215959705547
1102	28-29	0.4155968889526207
1102	30-31	0.4759004760060108
1102	32-33	0.46525893530721163
1102	34-35	0.4493097193851696
1102	36-37	0.4503530052094078
1102	38-39	0.4754209033131289
1102	40-41	0.4908614188178646
1102	42-43	0.5395194897574811
1102	44-45	0.5599270045137388
1102	46-47	0.5528138605286657
1102	48-49	0.576803558938316
1102	50-51	0.5307846723933025
1102	52-53	0.5350167813844067
1102	54-55	0.4550403285752225
1102	56-57	0.5000559328270882
1102	58-59	0.41294613139135805
1102	60-61	0.5271178211260406
1102	62-63	0.4361107051574038
1102	64-65	0.45758530974776335
1102	66-67	0.4522051132202165
1102	68-69	0.44753078826835946
1102	70-71	0.46007169008712623
1102	72-73	0.4183539537260188
1102	74-75	0.48253274470654617
1102	76-77	0.3884879204852254
1102	78-79	0.3841371378692884
1102	80-81	0.40659018638191924
1102	82-83	0.3747853290317096
1102	84-85	0.4225569095432036
1102	86-87	0.39423197935343524
1102	88-89	0.3861085339399004
1102	90-91	0.37068663660061674
1102	92-93	0.3071528439773772
1102	94-95	0.3573018683621889
1102	96-97	0.323422659456007
1102	98-99	0.3525458793259766
1102	100	0.4123080034502564
1103	1	0.36701283096349613
1103	2	0.4930948754706783
1103	3	0.48701645209093414
1103	4	0.43733323814225145
1103	5	0.3568265787957259
1103	6	0.3595097542809569
1103	7	0.3472898735129206
1103	8	0.4547610679088905
1103	9	0.532245085677971
1103	10-11	0.546232240310971
1103	12-13	0.49639539514313213
1103	14-15	0.5983098927822681
1103	16-17	0.581416580201271
1103	18-19	0.6268429915424676
1103	20-21	0.6019516155183879
1103	22-23	0.5931165855435978
1103	24-25	0.6163467078141096
1103	26-27	0.7016231612305077
1103	28-29	0.7021205562048252
1103	30-31	0.6750787548670161
1103	32-33	0.6877688287393866
1103	34-35	0.6537079256681153
1103	36-37	0.6702183087733715
1103	38-39	0.6489398823478751
1103	40-41	0.6939521968320292
1103	42-43	0.6443843108819038
1103	44-45	0.6745233668511545
1103	46-47	0.7070808843146423
1103	48-49	0.7440477102997889
1103	50-51	0.7019274313863519
1103	52-53	0.6155388494008491
1103	54-55	0.6591074649988684
1103	56-57	0.6723095345290986
1103	58-59	0.6883641259072775
1103	60-61	0.7071754983130845
1103	62-63	0.7155982140791082
1103	64-65	0.6945315564023389
1103	66-67	0.6696721790229176
1103	68-69	0.6722558148367526
1103	70-71	0.6837555368479045
1103	72-73	0.6320311831954797
1103	74-75	0.5997739535289668
1103	76-77	0.5917176650386153
1103	78-79	0.5922123546420437
1103	80-81	0.5882340289820789
1103	82-83	0.6055753905034038
1103	84-85	0.5724228871538983
1103	86-87	0.5804717258023686
1103	88-89	0.5728537983338775
1103	90-91	0.6022584383172713
1103	92-93	0.6057556682674239
1103	94-95	0.6119486336563789
1103	96-97	0.6252535346287083
1103	98-99	0.6218712954044179
1103	100	0.6106305166797092
1104	1	0.04350486532463549
1104	2	0.14328800014822463
1104	3	0.1666456805128682
1104	4	0.10144777211847611
1104	5	0.13557869384274568
1104	6	0.04454079507949871
1104	7	0.1207199125706353
1104	8	0.1258409294088807
1104	9	0.17466614206444575
1104	10-11	0.2265245069567925
1104	12-13	0.23746337880661628
1104	14-15	0.25746188581162954
1104	16-17	0.2569998754805951
1104	18-19	0.27665402417327556
1104	20-21	0.23923806822749327
1104	22-23	0.2722325765449298
1104	24-25	0.2436261783435114
1104	26-27	0.28576377084142024
1104	28-29	0.2271136186145526
1104	30-31	0.13662975087679285
1104	32-33	0.2172038505344389
1104	34-35	0.22685012685927575
1104	36-37	0.24833632938050698
1104	38-39	0.23803260684842797
1104	40-41	0.25642719724513796
1104	42-43	0.2414078551485801
1104	44-45	0.17266346315906844
1104	46-47	0.15875630580931954
1104	48-49	0.14522917930109003
1104	50-51	0.1860008748940416
1104	52-53	0.14780913581972044
1104	54-55	0.13922064607941564
1104	56-57	0.12282871283513686
1104	58-59	0.09382680285190048
1104	60-61	0.10308321839534784
1104	62-63	0.16030139013155065
1104	64-65	0.12992823050320368
1104	66-67	0.12990978689982313
1104	68-69	0.11698289213357782
1104	70-71	0.11621219395756555
1104	72-73	0.16730627242023388
1104	74-75	0.1697439120581734
1104	76-77	0.1517851314647416
1104	78-79	0.10686096503877707
1104	80-81	0.0772697839322305
1104	82-83	0.10868796202025521
1104	84-85	0.10109782314840032
1104	86-87	0.07164882241690407
1104	88-89	0.12601980503330523
1104	90-91	0.14136552979353212
1104	92-93	0.09805851677117161
1104	94-95	0.11945936200368834
1104	96-97	0.13915900976446594
1104	98-99	0.17645547539042994
1104	100	0.0984597669766849
1105	1	0.0196923694285438
1105	2	0.05346433261607331
1105	3	0.056232450432855074
1105	4	0.0018277904252812505
1105	5	0.07301452678387932
1105	6	0.10465058092263035
1105	7	0.0043559404253201706
1105	8	0.07657009336173815
1105	9	0.1233380553653447
1105	10-11	0.0984929150689382
1105	12-13	0.12390418617276566
1105	14-15	0.17069849459588227
1105	16-17	0.16828692712493165
1105	18-19	0.1529499308951685
1105	20-21	0.1349600883284907
1105	22-23	0.14052750351357446
1105	24-25	0.17591835360626362
1105	26-27	0.15040366279241368
1105	28-29	0.1689954676954457
1105	30-31	0.19087106031494017
1105	32-33	0.21836744027485366
1105	34-35	0.18196598571059042
1105	36-37	0.19527124551326835
1105	38-39	0.18454546437605046
1105	40-41	0.19647731947688385
1105	42-43	0.19383935754538584
1105	44-45	0.13443006409443115
1105	46-47	0.13587150478600307
1105	48-49	0.11187731862272443
1105	50-51	0.09422465135985902
1105	52-53	0.11658193771470593
1105	54-55	0.11312515706153192
1105	56-57	0.05615516752848748
1105	58-59	0.09740480459885958
1105	60-61	0.1075131468893602
1105	62-63	0.048101056895490046
1105	64-65	0.06275164169511527
1105	66-67	0.01412109647104387
1105	68-69	0.06773784317683607
1105	70-71	0.060818243749544365
1105	72-73	0.05442764967765612
1105	74-75	0.011947832683041781
1105	76-77	-0.026414627864078
1105	78-79	0.029390032290820045
1105	80-81	2.7389468279181983E-5
1105	82-83	0.00934003334993605
1105	84-85	-0.012401642148233805
1105	86-87	-0.06528701586596242
1105	88-89	-0.053557587819689445
1105	90-91	0.021513084430772267
1105	92-93	0.026507134798666954
1105	94-95	0.005062737123044059
1105	96-97	-0.0010084929287330624
1105	98-99	0.00885908337142638
1105	100	-0.0075160843112414
1106	1	0.014125629765977976
1106	2	0.06118991024137088
1106	3	0.054632578457784575
1106	4	0.1008648923019706
1106	5	0.03621709033068754
1106	6	0.10499977712508723
1106	7	0.11230393498718882
1106	8	0.08442727170459818
1106	9	0.1452652848580911
1106	10-11	0.1505440617317788
1106	12-13	0.1908499108420898
1106	14-15	0.1977481721081773
1106	16-17	0.06728633668029005
1106	18-19	0.12488582874252785
1106	20-21	0.18652595368160974
1106	22-23	0.16284743951283787
1106	24-25	0.09583864284646637
1106	26-27	0.1395265557337666
1106	28-29	0.18572051203008044
1106	30-31	0.1511732642166237
1106	32-33	0.20317281849429492
1106	34-35	0.16619572070220556
1106	36-37	0.19290442565925758
1106	38-39	0.14165346386781863
1106	40-41	0.1451397623173989
1106	42-43	0.11816384505365818
1106	44-45	0.10755088132022905
1106	46-47	0.100705785336757
1106	48-49	0.18261784817052984
1106	50-51	0.20197883528812355
1106	52-53	0.2512055589297333
1106	54-55	0.19626095009726896
1106	56-57	0.14796681847121107
1106	58-59	0.16242250548156534
1106	60-61	0.26948002659384684
1106	62-63	0.22870763948131412
1106	64-65	0.19886375788595245
1106	66-67	0.16972335220700074
1106	68-69	0.17940544848782025
1106	70-71	0.1856379778779953
1106	72-73	0.2012599339422323
1106	74-75	0.20315875126337346
1106	76-77	0.16674613560180518
1106	78-79	0.22941419816868347
1106	80-81	0.20435508932367696
1106	82-83	0.22430639090146798
1106	84-85	0.2118610311254301
1106	86-87	0.16510640433264712
1106	88-89	0.1385531836404219
1106	90-91	0.12457050029973615
1106	92-93	0.13646236271044998
1106	94-95	0.17901391384301846
1106	96-97	0.20046934795779237
1106	98-99	0.21143847209049227
1106	100	0.27813294536794686
1107	1	0.01154571248067171
1107	2	0.16326416519559572
1107	3	0.11970512050853088
1107	4	0.13209573980537215
1107	5	0.07658205027608744
1107	6	0.02787824502621561
1107	7	0.048936227784423636
1107	8	0.022991245595846976
1107	9	0.09843370803994844
1107	10-11	0.08699182658044435
1107	12-13	0.11086754416643885
1107	14-15	0.1645110646905792
1107	16-17	0.18620632778237933
1107	18-19	0.11301467079848493
1107	20-21	0.15403527883574242
1107	22-23	0.14754457722141723
1107	24-25	0.17140875618274265
1107	26-27	0.16353077999917076
1107	28-29	0.13280685312368945
1107	30-31	0.1488202912336547
1107	32-33	0.10616272317796671
1107	34-35	0.15071467610563616
1107	36-37	0.16935507056153654
1107	38-39	0.1214114777290618
1107	40-41	0.105890080720215
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2216	74-75	-0.31781397078127327
2216	76-77	-0.2828577793203735
2216	78-79	-0.2516358885362564
2216	80-81	-0.20792073888766538
2216	82-83	-0.2564454666060385
2216	84-85	-0.32694339499773406
2216	86-87	-0.26397275183734337
2216	88-89	-0.21082571305261766
2216	90-91	-0.2954802027120209
2216	92-93	-0.3657243610790921
2216	94-95	-0.3563816388360017
2216	96-97	-0.2678648228200373
2216	98-99	-0.24161240609764434
2216	100	-0.28109691155662375
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	63375.0
3	9386.0
4	6144.0
5	4971.0
6	4316.0
7	4741.0
8	5407.0
9	5791.0
10	6307.0
11	6791.0
12	7508.0
13	7912.0
14	8433.0
15	8595.0
16	8646.0
17	8538.0
18	8363.0
19	8305.0
20	8353.0
21	8828.0
22	10414.0
23	14055.0
24	20607.0
25	26422.0
26	27361.0
27	26009.0
28	29482.0
29	36156.0
30	44460.0
31	54202.0
32	70286.0
33	94630.0
34	136560.0
35	217302.0
36	411975.0
37	844063.0
38	1225135.0
39	451766.0
40	512.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.317771315640165	18.712119385101232	12.668432683410401	24.3016766158482
2	15.949744674207336	19.345228650816114	39.878550475554604	24.826476199421947
3	18.953261926709498	24.423532959959505	29.074898255588867	27.548306857742134
4	12.616799059032127	15.73171143482622	37.81157945966448	33.83991004647717
5	13.370381465440643	37.86063538646668	35.18936182096054	13.57962132713213
6	32.38640575574635	37.07363760529269	17.12865128889927	13.411305350061689
7	28.957301358094647	31.76678730495287	21.34528444436321	17.930626892589274
8	26.22335334661911	36.059049770883846	19.405338850738122	18.312258031758926
9	25.67332603041585	16.649204823987418	20.05468302757126	37.622786118025466
10-11	24.835983067090623	26.402086784333882	28.03933664629386	20.722593502281626
12-13	25.30649526507242	23.64209263413682	28.079226103422307	22.972185997368456
14-15	22.381692562879742	25.661431067230637	25.046595921086233	26.91028044880338
16-17	22.490432480685676	28.999315730866325	25.660968316346338	22.849283472101657
18-19	22.455864228479168	28.123253957273754	27.337917098748115	22.082964715498957
20-21	23.442229603146156	27.34375079329137	26.970180367802882	22.243839235759594
22-23	23.202947922797648	27.06259588764719	26.915763316760938	22.818692872794227
24-25	23.002722528878873	27.15041258072611	26.95596799172211	22.890896898672906
26-27	22.75338498428043	27.12951632165782	27.097123945391427	23.019974748670318
28-29	22.737993744436775	27.32335484309878	27.16117169392544	22.777479718539002
30-31	22.91771754878145	27.568148568054383	26.808340172793578	22.705793710370585
32-33	22.680202873010423	27.28815375050803	27.209800536300015	22.821842840181528
34-35	22.837878471142034	27.08093364856544	27.258070841930948	22.823117038361577
36-37	23.05973500354999	27.33364488282498	26.853806204877888	22.75281390874714
38-39	22.839903081564554	27.61373256553354	26.922001982537928	22.624362370363976
40-41	22.705170834437062	27.297729429607546	27.188940969536056	22.808158766419336
42-43	22.63899089357971	27.28257726596175	27.08631361210676	22.992118228351785
44-45	22.684330966607227	27.309296948304368	27.122577084458815	22.883795000629593
46-47	22.804774749078398	27.336335925788973	27.08734695556242	22.77154236957021
48-49	22.71390456131193	27.28759335645543	27.055669986881796	22.942832095350845
50-51	22.613337585090147	27.309531619563902	27.08300116483242	22.994129630513534
52-53	22.706113510066135	27.30707594689892	27.065806631135953	22.921003911898996
54-55	22.739421257719457	27.527072054368546	26.96059763153165	22.772909056380353
56-57	22.645338025075944	27.2859487640381	27.06618835264742	23.00252485823854
58-59	22.66662096839711	26.94260805101958	27.294015049963445	23.09675593061987
60-61	22.83446176062075	27.38595158167264	26.94852680769025	22.83105985001636
62-63	22.553830554155006	27.328904216216877	27.063667479576964	23.053597750051154
64-65	22.608312414190895	27.092066567554614	27.11881209735728	23.18080892089721
66-67	22.473536744546426	27.14911489580032	26.978703602272606	23.398644757380648
68-69	22.782586509571555	27.276260689537867	26.982646676897648	22.958506123992926
70-71	22.755421807868412	27.05241461737883	27.061401460031902	23.130762114720856
72-73	22.571958860559455	27.099183542599043	27.189229697917618	23.13962789892389
74-75	22.579808636976374	27.25016564655468	27.080914216663153	23.089111499805796
76-77	22.58993247733886	27.080857104705057	27.03673559742501	23.292474820531073
78-79	22.62936604203911	27.137077713809315	27.16812546235251	23.065430781799062
80-81	22.617443718128477	27.011172978710384	27.205788085828665	23.165595217332474
82-83	22.514859422865676	27.014028391887145	27.300579855768465	23.170532329478714
84-85	22.471909969739436	27.002845813278093	27.15199079997563	23.373253417006843
86-87	22.624189023009457	27.026452396450335	27.196784220461858	23.152574360078354
88-89	22.34047684386117	26.98899241545159	27.197029950043067	23.473500790644174
90-91	22.392678489674488	26.828432437111548	27.317593382473245	23.46129569074072
92-93	22.617736653720204	27.01790927322113	27.149062016094227	23.215292056964433
94-95	22.385856682224414	26.757595634962744	27.31783447930623	23.53871320350661
96-97	22.41816194865377	26.720867862215325	27.385865888531946	23.475104300598957
98-99	22.30824042664939	26.90011821756245	27.27846772574236	23.51317363004579
100	22.427149814717318	26.525402689657728	27.245768756857032	23.80167873876792
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3033.0
1	1742.5
2	391.5
3	303.5
4	255.0
5	221.5
6	205.5
7	208.0
8	222.0
9	219.5
10	205.5
11	216.0
12	239.0
13	265.0
14	303.0
15	340.0
16	431.5
17	555.0
18	745.0
19	1046.5
20	1470.0
21	2062.5
22	2986.5
23	4651.5
24	7030.5
25	9935.0
26	13988.5
27	19064.5
28	25825.0
29	34552.5
30	44233.0
31	54205.5
32	63826.0
33	74843.5
34	86066.0
35	95522.0
36	104478.0
37	113767.5
38	122091.5
39	127495.0
40	132880.0
41	138447.0
42	142292.0
43	144696.5
44	144010.0
45	143264.0
46	143668.5
47	142888.5
48	143359.0
49	141574.5
50	136459.5
51	132647.5
52	126257.5
53	120854.0
54	117428.0
55	110778.0
56	102355.5
57	93814.5
58	85640.5
59	79064.5
60	71598.0
61	61923.0
62	54050.0
63	46137.0
64	37673.5
65	30665.5
66	24416.5
67	18712.0
68	14428.0
69	11277.5
70	8287.5
71	5953.5
72	4698.0
73	3610.0
74	2699.5
75	1935.5
76	1187.5
77	779.0
78	562.0
79	391.0
80	278.5
81	179.5
82	130.0
83	103.5
84	83.0
85	62.0
86	41.0
87	35.0
88	24.5
89	15.5
90	12.5
91	8.5
92	11.5
93	9.0
94	3.5
95	4.5
96	4.0
97	3.0
98	2.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07412279778301299
2	0.07665951228619619
3	0.07787713524772412
4	0.08076898978135297
5	0.07830837671326527
6	0.07830837671326527
7	0.07759809665237398
8	0.0775473623623103
9	0.07716685518683282
10-11	0.0769131837365145
12-13	0.07549262361473193
14-15	0.07409743063798116
16-17	0.07274028837877815
18-19	0.07209342618046644
20-21	0.07161145042486163
22-23	0.07423694993565624
24-25	0.07454135567603822
26-27	0.07378034132508327
28-29	0.06934109094451267
30-31	0.07498528071409528
32-33	0.07341251772212169
34-35	0.07071091677623159
36-37	0.06742587149460937
38-39	0.06879569732632827
40-41	0.07129436111196373
42-43	0.07655804370606886
44-45	0.07739515949211932
46-47	0.08060410333864605
48-49	0.08386378147523647
50-51	0.08480236584141425
52-53	0.08510677158179623
54-55	0.08520824016192356
56-57	0.08300129854415418
58-59	0.08185977701772174
60-61	0.07975430398007968
62-63	0.08048995118600281
64-65	0.07942453109466587
66-67	0.07882840318641782
68-69	0.07843521243842443
70-71	0.07648194227097337
72-73	0.0765073094160052
74-75	0.07602533366040039
76-77	0.0757209279200184
78-79	0.0764312079809097
80-81	0.07736979234708748
82-83	0.07671024657625986
84-85	0.07577166221008207
86-87	0.07688781659148268
88-89	0.07391986062275833
90-91	0.07129436111196373
92-93	0.07120557610435232
94-95	0.07115484181428865
96-97	0.0703177260282382
98-99	0.07001332028785621
100	0.06902400163161478
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3942107.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.86336076382112
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.56923766075258	39.08823028476591
2	14.756869221482788	15.89709141242511
3	5.223377925106138	8.44046068757314
4	2.466751452332891	5.314700935667449
5	1.3710896484442556	3.6925748186846805
6	0.8362377358944934	2.702554491168364
7	0.5529013898749999	2.0846788920758668
8	0.3817680896668413	1.6450649873471908
9	0.2902001348440428	1.4068039101132792
>10	1.4369027761122422	13.759224760632602
>50	0.08397393240107641	3.08148827095657
>100	0.029933283741720954	2.515360204464803
>500	6.14858867028696E-4	0.21205943044215603
>1k	1.4189047891393855E-4	0.15970691368289774
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	2.5367145031831962E-5	0.0	0.0	0.0
9	0.0	2.5367145031831962E-5	0.0	2.5367145031831962E-5	0.0
10-11	0.0	2.5367145031831962E-5	0.0	2.5367145031831962E-5	0.0
12-13	0.0	2.5367145031831962E-5	0.0	2.5367145031831962E-5	0.0
14-15	0.0	2.5367145031831962E-5	0.0	2.5367145031831962E-5	0.0
16-17	0.0	2.5367145031831962E-5	0.0	5.0734290063663924E-5	0.0
18-19	0.0	2.5367145031831962E-5	0.0	6.34178625795799E-5	0.0
20-21	0.0	2.5367145031831962E-5	0.0	1.0146858012732785E-4	0.0
22-23	0.0	2.5367145031831962E-5	0.0	1.3951929767507578E-4	0.0
24-25	0.0	2.5367145031831962E-5	0.0	2.663550228342356E-4	0.0
26-27	0.0	2.5367145031831962E-5	0.0	4.946593281207232E-4	0.0
28-29	0.0	2.5367145031831962E-5	0.0	0.0015727629919735817	0.0
30-31	0.0	2.5367145031831962E-5	0.0	0.005796392639773603	0.0
32-33	0.0	2.5367145031831962E-5	0.0	0.01558811062206074	0.0
34-35	0.0	2.5367145031831962E-5	0.0	0.0299205475650458	0.0
36-37	0.0	2.5367145031831962E-5	0.0	0.053752980322451925	0.0
38-39	0.0	2.5367145031831962E-5	0.0	0.09739715334971882	0.0
40-41	0.0	2.5367145031831962E-5	0.0	0.1518096794429984	0.0
42-43	0.0	2.5367145031831962E-5	0.0	0.20732567634516263	0.0
44-45	0.0	2.5367145031831962E-5	0.0	0.2698937395661761	0.0
46-47	0.0	2.5367145031831962E-5	0.0	0.3489250799128486	0.0
48-49	0.0	2.5367145031831962E-5	0.0	0.43996776343209354	0.0
50-51	0.0	2.5367145031831962E-5	0.0	0.5385698561708244	0.0
52-53	0.0	2.5367145031831962E-5	0.0	0.6388842311992039	0.0
54-55	0.0	2.5367145031831962E-5	0.0	0.7473287762102855	0.0
56-57	0.0	2.5367145031831962E-5	0.0	0.876028986529285	0.0
58-59	0.0	2.5367145031831962E-5	0.0	1.0214968797143253	0.0
60-61	0.0	2.5367145031831962E-5	0.0	1.1814367291400258	0.0
62-63	0.0	2.5367145031831962E-5	0.0	1.3473251740756909	0.0
64-65	0.0	2.5367145031831962E-5	0.0	1.519555405269314	0.0
66-67	0.0	2.5367145031831962E-5	0.0	1.7160619942584003	0.0
68-69	0.0	2.5367145031831962E-5	0.0	1.9420578893469913	0.0
70-71	0.0	2.5367145031831962E-5	0.0	2.1856458995151575	0.0
72-73	0.0	2.5367145031831962E-5	0.0	2.438949526230516	0.0
74-75	0.0	2.5367145031831962E-5	0.0	2.689031525526831	0.0
76-77	0.0	2.5367145031831962E-5	0.0	2.9596228615813827	0.0
78-79	0.0	2.5367145031831962E-5	0.0	3.271613378327884	0.0
80-81	0.0	2.5367145031831962E-5	0.0	3.613981051250004	0.0
82-83	0.0	2.5367145031831962E-5	0.0	3.9593927815759438	0.0
84-85	0.0	2.5367145031831962E-5	0.0	4.3034981039327445	0.0
86-87	0.0	2.5367145031831962E-5	0.0	4.668658156665966	0.0
88	0.0	2.5367145031831962E-5	0.0	4.966531857202252	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	12535	0.0	30.034317	1
TACATGG	12465	0.0	28.506186	2
ACATGGG	12770	0.0	26.500324	3
GTATCAA	7780	0.0	22.896505	1
TCAACGC	7720	0.0	21.978575	4
CAACGCA	7960	0.0	21.31536	5
ATCAACG	7995	0.0	21.222586	3
CATGGGG	9215	0.0	21.014132	4
AACGCAG	8165	0.0	20.664543	6
TATCAAC	8920	0.0	19.70681	2
TACACCG	880	0.0	19.227304	5
GAGTACA	8505	0.0	18.568401	1
ACACCGT	915	0.0	18.491364	6
GGTATCA	6005	0.0	18.002151	1
CATGGGA	4360	0.0	17.89498	4
ACGCAGA	9685	0.0	17.27579	7
AGTACAT	8700	0.0	16.855627	2
CGCAGAG	9995	0.0	16.645716	8
AGAGTAC	7425	0.0	15.5395	10-11
GTATTAG	1805	0.0	15.363295	1
>>END_MODULE