FastQCFastQC Report
Wed 25 May 2016
SRR1032947_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032947_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3942107
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53070.13462343868393223No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43980.11156470384999698No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA125750.031.49451
GGTATCA81700.030.8217931
GTACATG124900.029.1510641
TCAACGC135700.028.9892044
ATCAACG136500.028.8541413
CAACGCA137800.028.6480465
AACGCAG141650.028.0019156
TATCAAC145450.027.0776232
TACATGG127400.026.9989432
ACATGGG129850.025.6932853
ACGCAGA164750.023.7326817
CGCAGAG168100.023.1754578
GAGTACA85700.020.8861921
GCAGAGT185050.020.8672319
CATGGGG92300.020.3923914
GAGTACT95550.020.0091812-13
AGAGTAC139550.019.25447810-11
AGTACAT88050.018.917892
GTACTTT103600.018.2503714-15
AGTACTT99850.017.40681312-13
Produced by FastQC (version 0.11.5)