FastQCFastQC Report
Wed 25 May 2016
SRR1032946_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032946_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2644855
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33150.035.5885731
TCAACGC32700.033.6347734
ATCAACG34350.032.019133
CAACGCA34500.031.8781075
GGTATCA21750.030.686651
AACGCAG36100.030.4640736
GTACATG83250.028.3990731
TATCAAC40850.027.4995922
TACATGG83200.027.229782
ACATGGG87050.024.945583
ACGCAGA45350.024.146717
GAGTACA60400.022.3339421
CGCAGAG48950.021.9868038
CATGGGG57900.020.3758434
AGTACAT61700.019.8065382
TACGACC2652.0962034E-819.5104474
GTACGAC2451.9642539E-719.184673
TACCTGG17550.018.4795912
GCAGAGT57300.018.3723419
GTATAGG11000.017.9463861
Produced by FastQC (version 0.11.5)