##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1032946_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2644855
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.36956203648215	33.0	31.0	34.0	30.0	34.0
2	31.472231559007962	33.0	31.0	34.0	28.0	34.0
3	31.56971440778417	34.0	31.0	34.0	28.0	34.0
4	34.90843694644886	37.0	35.0	37.0	32.0	37.0
5	34.940269315331086	37.0	35.0	37.0	32.0	37.0
6	35.047742503842365	37.0	35.0	37.0	33.0	37.0
7	34.98040346257167	37.0	35.0	37.0	32.0	37.0
8	34.97923440037356	37.0	36.0	37.0	32.0	37.0
9	36.59391081930767	39.0	38.0	39.0	33.0	39.0
10-11	36.5823071586155	39.0	38.0	39.0	33.0	39.0
12-13	36.51268387113849	39.0	38.0	39.0	33.0	39.0
14-15	37.94327042503275	41.0	39.0	41.0	33.0	41.0
16-17	37.95647360630356	41.0	39.0	41.0	33.0	41.0
18-19	37.963578154568026	41.0	39.0	41.0	33.0	41.0
20-21	37.99355673562445	41.0	39.0	41.0	33.0	41.0
22-23	37.97682859740893	41.0	39.0	41.0	33.0	41.0
24-25	38.00242111571333	41.0	39.0	41.0	33.0	41.0
26-27	37.973756028213266	41.0	39.0	41.0	33.0	41.0
28-29	37.953845863005725	41.0	39.0	41.0	33.0	41.0
30-31	37.9043930574644	41.0	38.5	41.0	32.5	41.0
32-33	37.85077404999518	41.0	38.0	41.0	32.0	41.0
34-35	37.775648381480266	40.5	38.0	41.0	32.0	41.0
36-37	37.68555988891641	40.0	38.0	41.0	32.0	41.0
38-39	37.585559321777566	40.0	38.0	41.0	31.5	41.0
40-41	37.500623474632825	40.0	38.0	41.0	31.0	41.0
42-43	37.38435074890684	40.0	38.0	41.0	31.0	41.0
44-45	37.246562287913704	40.0	38.0	41.0	31.0	41.0
46-47	37.12098508235801	40.0	37.5	41.0	31.0	41.0
48-49	36.98023143045649	40.0	37.0	41.0	30.0	41.0
50-51	36.83625642993661	40.0	37.0	41.0	30.0	41.0
52-53	36.6702055500207	40.0	36.5	41.0	30.0	41.0
54-55	36.49506456875707	40.0	36.0	41.0	30.0	41.0
56-57	36.3104100224776	40.0	35.5	41.0	30.0	41.0
58-59	36.093677347151356	39.0	35.0	41.0	29.5	41.0
60-61	35.8441842747523	39.0	35.0	41.0	29.0	41.0
62-63	35.58197708380989	39.0	35.0	41.0	29.0	41.0
64-65	35.299110915343185	38.0	35.0	40.0	28.5	41.0
66-67	34.99966066192664	37.5	34.5	40.0	28.0	41.0
68-69	34.68574250762329	37.0	34.0	40.0	28.0	41.0
70-71	34.345711201559254	36.5	34.0	39.0	28.0	41.0
72-73	34.04870153562294	36.0	34.0	39.0	27.5	41.0
74-75	33.77865270496871	35.5	34.0	39.0	27.0	40.5
76-77	33.45800185643448	35.0	34.0	37.5	27.0	40.0
78-79	33.095537373504406	35.0	34.0	37.0	26.0	39.0
80-81	32.78109575005057	35.0	33.5	37.0	26.0	39.0
82-83	32.46769860729606	35.0	33.0	36.0	26.0	38.5
84-85	32.31201029924136	35.0	33.0	36.0	26.0	37.0
86-87	32.08525439012725	35.0	33.0	36.0	26.0	37.0
88-89	31.856358099026224	35.0	33.0	35.0	25.0	37.0
90-91	31.660724311918802	35.0	33.0	35.0	25.0	36.0
92-93	31.469150104637116	35.0	33.0	35.0	25.0	36.0
94-95	31.289255554652335	35.0	33.0	35.0	24.0	36.0
96-97	31.11453728087173	35.0	33.0	35.0	23.5	36.0
98-99	30.930408661344384	35.0	33.0	35.0	21.5	35.0
100	30.795806197315166	35.0	33.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5326644143048753
1101	2	0.5649738947407101
1101	3	0.5534095334059543
1101	4	0.4932506559682821
1101	5	0.48711280193916195
1101	6	0.4897980013560428
1101	7	0.4221294876144128
1101	8	0.48858397805504694
1101	9	0.6175957544080433
1101	10-11	0.5813414675056237
1101	12-13	0.38498064758826445
1101	14-15	0.5040039836539307
1101	16-17	0.48811344875583984
1101	18-19	0.581788551966909
1101	20-21	0.4512961460020719
1101	22-23	0.5305716106337712
1101	24-25	0.525437727895401
1101	26-27	0.5556250094742907
1101	28-29	0.5266439886986376
1101	30-31	0.539925644603656
1101	32-33	0.5891251378325677
1101	34-35	0.6040550243302434
1101	36-37	0.495517963966023
1101	38-39	0.5438996780741405
1101	40-41	0.5344148626402472
1101	42-43	0.6041726383632309
1101	44-45	0.5698462230616741
1101	46-47	0.6029276634577201
1101	48-49	0.6284133110174039
1101	50-51	0.5965599633355865
1101	52-53	0.545419184443638
1101	54-55	0.513328865783329
1101	56-57	0.6738458695671667
1101	58-59	0.5778826103755392
1101	60-61	0.5964645398474175
1101	62-63	0.671724607704256
1101	64-65	0.6058240625773834
1101	66-67	0.6314022750600685
1101	68-69	0.7169977795430214
1101	70-71	0.6585722717568459
1101	72-73	0.5691128940546264
1101	74-75	0.6229827201674141
1101	76-77	0.6072053095640442
1101	78-79	0.6143869628228558
1101	80-81	0.4929920927454674
1101	82-83	0.4348130071701135
1101	84-85	0.43654075604717235
1101	86-87	0.46272214506151954
1101	88-89	0.5440127772889021
1101	90-91	0.5732392844106506
1101	92-93	0.5299815974370148
1101	94-95	0.5350760933820737
1101	96-97	0.5005277220122544
1101	98-99	0.5834217518639733
1101	100	0.5982994285698027
1102	1	0.22539974422247155
1102	2	0.20924269286715358
1102	3	0.2694280746407678
1102	4	0.1850795465875663
1102	5	0.11513253785338406
1102	6	0.11854426353839642
1102	7	0.16993313826075962
1102	8	0.11286204442849623
1102	9	0.12005160282598126
1102	10-11	0.19793044445471253
1102	12-13	0.21417965534063654
1102	14-15	0.24826531503406102
1102	16-17	0.23916371181050522
1102	18-19	0.21567608380428993
1102	20-21	0.24915911579967087
1102	22-23	0.24668520473433375
1102	24-25	0.20053802526153675
1102	26-27	0.26765156738720464
1102	28-29	0.2315080405818577
1102	30-31	0.2582858997056334
1102	32-33	0.2445268547101378
1102	34-35	0.2426992291276875
1102	36-37	0.22328963797499313
1102	38-39	0.2690693687730601
1102	40-41	0.2274275288494252
1102	42-43	0.25388398343238805
1102	44-45	0.1546388312198701
1102	46-47	0.1686631201914821
1102	48-49	0.14661214217530727
1102	50-51	0.14610431403087887
1102	52-53	0.13085488094291975
1102	54-55	0.07047495682311222
1102	56-57	0.14901964919858557
1102	58-59	0.1040267707280691
1102	60-61	0.17712981509183834
1102	62-63	0.20626044275316247
1102	64-65	0.14768667207484754
1102	66-67	0.13839956451264612
1102	68-69	0.25334009312028627
1102	70-71	0.18851771710085075
1102	72-73	0.20375990065973326
1102	74-75	0.11791682909420587
1102	76-77	0.07867582006429785
1102	78-79	0.07435512840921632
1102	80-81	0.11397839067338822
1102	82-83	0.06210906129098959
1102	84-85	0.10767668520077933
1102	86-87	0.1009630270383397
1102	88-89	0.11559718530036278
1102	90-91	0.09933366645443087
1102	92-93	0.13219448030035963
1102	94-95	0.18826587555456697
1102	96-97	0.15240146051731784
1102	98-99	0.18262387668664104
1102	100	0.25772074387864663
1103	1	0.30592170454467293
1103	2	0.37459132849082266
1103	3	0.42142081072633175
1103	4	0.40500256778844346
1103	5	0.3494304985494594
1103	6	0.3613012128278612
1103	7	0.27292605349617816
1103	8	0.34498795227870716
1103	9	0.37376517893518013
1103	10-11	0.4417432651301638
1103	12-13	0.4067314724876212
1103	14-15	0.46136773422468025
1103	16-17	0.49591538007393865
1103	18-19	0.42115751286372927
1103	20-21	0.45063893807412114
1103	22-23	0.4596414109413729
1103	24-25	0.42433157341890393
1103	26-27	0.4712731761973643
1103	28-29	0.5402313030230346
1103	30-31	0.47066971657665135
1103	32-33	0.4476808935378074
1103	34-35	0.4534510019879292
1103	36-37	0.46877867812124663
1103	38-39	0.5252557691852218
1103	40-41	0.5122860282858213
1103	42-43	0.49621152944664004
1103	44-45	0.5075106255780923
1103	46-47	0.44156197619172133
1103	48-49	0.44098438885908564
1103	50-51	0.36424718659302613
1103	52-53	0.4556364238348394
1103	54-55	0.4703324158551041
1103	56-57	0.34333002798368994
1103	58-59	0.3724715831158889
1103	60-61	0.4463465296616107
1103	62-63	0.5037691470991632
1103	64-65	0.460789647631465
1103	66-67	0.46382126211882024
1103	68-69	0.49757029956545296
1103	70-71	0.5065222770765487
1103	72-73	0.4900026386925802
1103	74-75	0.45172329606998574
1103	76-77	0.3862966989222869
1103	78-79	0.43044216185460016
1103	80-81	0.3989269101278339
1103	82-83	0.36706038483006154
1103	84-85	0.3988128847091801
1103	86-87	0.37958499748640406
1103	88-89	0.36623194326446296
1103	90-91	0.3064552624681802
1103	92-93	0.3741755215727025
1103	94-95	0.4175009056929113
1103	96-97	0.4302802372687857
1103	98-99	0.484127400152488
1103	100	0.5241820002198274
1104	1	0.12597793258026257
1104	2	0.16495520043369538
1104	3	0.2036571989430236
1104	4	0.09909525863372437
1104	5	0.12222284311881992
1104	6	0.15563159162266516
1104	7	0.1538473514994365
1104	8	0.18195242220092211
1104	9	0.1925770544292149
1104	10-11	0.15648577553153586
1104	12-13	0.1422720512244382
1104	14-15	0.18846835659265082
1104	16-17	0.24406913961824728
1104	18-19	0.22801512651420097
1104	20-21	0.14319354783370386
1104	22-23	0.2579245580158869
1104	24-25	0.3052187912430071
1104	26-27	0.36457803980103876
1104	28-29	0.37151311607152593
1104	30-31	0.2848377416579666
1104	32-33	0.33247827941766417
1104	34-35	0.36412142088232713
1104	36-37	0.30719879769581837
1104	38-39	0.36086619098920636
1104	40-41	0.42607318606491873
1104	42-43	0.3762975435588203
1104	44-45	0.3573782773323373
1104	46-47	0.3084116747205812
1104	48-49	0.3187660503572829
1104	50-51	0.41610806022563906
1104	52-53	0.3421752003691907
1104	54-55	0.27626134462347807
1104	56-57	0.251569039096168
1104	58-59	0.30447587466245096
1104	60-61	0.3402577009526411
1104	62-63	0.2753847669824765
1104	64-65	0.22743733589835813
1104	66-67	0.35024874556810204
1104	68-69	0.22698709610955348
1104	70-71	0.3395586868489602
1104	72-73	0.38253973976011935
1104	74-75	0.31161882754079784
1104	76-77	0.3410978613120008
1104	78-79	0.34610255798130396
1104	80-81	0.3121441834110428
1104	82-83	0.32061155014853426
1104	84-85	0.3456679338261708
1104	86-87	0.2744228301488931
1104	88-89	0.2939782359267191
1104	90-91	0.2148754310330041
1104	92-93	0.2018177844880782
1104	94-95	0.2574529293206993
1104	96-97	0.31350354487371135
1104	98-99	0.33353422398415944
1104	100	0.3248879285308952
1105	1	0.025739322535681453
1105	2	0.030501500458111508
1105	3	0.11763259861790232
1105	4	0.05898738404085435
1105	5	-0.013627193121195091
1105	6	0.030696984453939535
1105	7	0.022901896347015338
1105	8	0.09319485953270856
1105	9	0.1643206339218608
1105	10-11	0.12105711000126007
1105	12-13	0.14473794619124192
1105	14-15	0.22408992673440764
1105	16-17	0.20145034993557687
1105	18-19	0.17105985337877883
1105	20-21	0.2673500566442826
1105	22-23	0.3432452906001515
1105	24-25	0.2438109363409353
1105	26-27	0.14194236977743913
1105	28-29	0.17772486168930612
1105	30-31	0.24945562293158474
1105	32-33	0.24014679072195833
1105	34-35	0.23613016109646878
1105	36-37	0.20353709769526773
1105	38-39	0.15560771170179066
1105	40-41	0.17323830929112916
1105	42-43	0.1536744940972099
1105	44-45	0.14381557003451917
1105	46-47	0.12547031840100686
1105	48-49	0.13347099315289057
1105	50-51	0.15574291479808267
1105	52-53	0.10423038153735575
1105	54-55	0.1276816735261832
1105	56-57	0.115823529607475
1105	58-59	0.12161292188791606
1105	60-61	0.15621930567473896
1105	62-63	0.08526234481707462
1105	64-65	0.08052364683616986
1105	66-67	0.09601753252989198
1105	68-69	0.04930793739350747
1105	70-71	0.0258925995067969
1105	72-73	0.03318287274270659
1105	74-75	0.03437748019130993
1105	76-77	-0.025147590705650202
1105	78-79	0.06140188174988026
1105	80-81	0.07164733088421116
1105	82-83	0.043982775022932685
1105	84-85	-0.022784623785000235
1105	86-87	-0.06606076400260719
1105	88-89	0.02033245954706686
1105	90-91	0.05193736013068673
1105	92-93	0.04476557729427455
1105	94-95	-0.0434043393155612
1105	96-97	0.014634184382018134
1105	98-99	0.028147327085875418
1105	100	-0.05073114056831329
1106	1	-0.03268980536200061
1106	2	0.014395965581922354
1106	3	0.05379896077915092
1106	4	0.07564434961196298
1106	5	0.01695352621525359
1106	6	0.051480026338218465
1106	7	0.11583714751957785
1106	8	0.098761941128231
1106	9	0.10057787144972963
1106	10-11	0.14691321130387536
1106	12-13	0.19308570073300757
1106	14-15	0.24298678988666467
1106	16-17	0.226065732174618
1106	18-19	0.27021684919877487
1106	20-21	0.21874660250506395
1106	22-23	0.23890176987220002
1106	24-25	0.2048757330517077
1106	26-27	0.25642435058239954
1106	28-29	0.19578601900898462
1106	30-31	0.16923249687560826
1106	32-33	0.2643981566199045
1106	34-35	0.189768857114764
1106	36-37	0.22419019810276097
1106	38-39	0.23912674314028948
1106	40-41	0.21762487610988757
1106	42-43	0.22835499510108548
1106	44-45	0.19232866368170676
1106	46-47	0.16438029283489897
1106	48-49	0.2434835406468565
1106	50-51	0.20600523053412445
1106	52-53	0.1771939044741231
1106	54-55	0.1958387133679551
1106	56-57	0.21742199641389703
1106	58-59	0.17620082784401347
1106	60-61	0.25040505852824424
1106	62-63	0.2078893843318994
1106	64-65	0.21989292802746263
1106	66-67	0.3198126036868487
1106	68-69	0.31011650676036595
1106	70-71	0.26787683868046486
1106	72-73	0.22079377994917593
1106	74-75	0.22446538473150213
1106	76-77	0.24072940420816025
1106	78-79	0.24094451092097557
1106	80-81	0.2433077448279377
1106	82-83	0.24952039291955685
1106	84-85	0.2284326041250111
1106	86-87	0.29748916506054
1106	88-89	0.2062946739300351
1106	90-91	0.17424601406570517
1106	92-93	0.12371104203424466
1106	94-95	0.16772166311310244
1106	96-97	0.16338081227519297
1106	98-99	0.14366575045747254
1106	100	0.16074639249243106
1107	1	0.12418383511999664
1107	2	0.1587418058957084
1107	3	0.27184381235369415
1107	4	0.11753964813892281
1107	5	0.12158305421019122
1107	6	0.17466823395436393
1107	7	0.1577036187519667
1107	8	0.13044473937647183
1107	9	0.0729263978760386
1107	10-11	0.14881288689825567
1107	12-13	0.22019518421072348
1107	14-15	0.23936089523958515
1107	16-17	0.30472245293188394
1107	18-19	0.2658677775177267
1107	20-21	0.24390506946953394
1107	22-23	0.2775742015050042
1107	24-25	0.2975974325020303
1107	26-27	0.38443614069949206
1107	28-29	0.3866742039698323
1107	30-31	0.314627669190763
1107	32-33	0.23717145446363475
1107	34-35	0.29377250019491186
1107	36-37	0.27124807702738707
1107	38-39	0.3145565008173179
1107	40-41	0.34916662388410913
1107	42-43	0.30489201655413467
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2216	70-71	-0.360097631868058
2216	72-73	-0.49118471114222473
2216	74-75	-0.518781822935793
2216	76-77	-0.44102270841178637
2216	78-79	-0.3810531586992454
2216	80-81	-0.3428286437032497
2216	82-83	-0.3159439448740713
2216	84-85	-0.3997207735326782
2216	86-87	-0.4455899885242154
2216	88-89	-0.34202579782082054
2216	90-91	-0.3318538344506585
2216	92-93	-0.33231224229524514
2216	94-95	-0.3608689852254514
2216	96-97	-0.37579122934359077
2216	98-99	-0.3431388992029092
2216	100	-0.4406097994781426
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	45931.0
3	6976.0
4	4359.0
5	3715.0
6	3134.0
7	3401.0
8	3802.0
9	4242.0
10	4393.0
11	4902.0
12	5208.0
13	5717.0
14	5920.0
15	6133.0
16	6082.0
17	6161.0
18	6118.0
19	5889.0
20	6128.0
21	6359.0
22	7491.0
23	10443.0
24	15897.0
25	20521.0
26	20974.0
27	18634.0
28	20310.0
29	24578.0
30	30227.0
31	36166.0
32	46771.0
33	62144.0
34	89281.0
35	140038.0
36	263235.0
37	539797.0
38	817600.0
39	335634.0
40	544.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.17007500036893	19.04024047340932	12.741586817250122	24.04809770897163
2	15.830233001655863	19.440631044889976	40.379751529480146	24.349384423974016
3	18.912008464115676	24.546162502847483	29.0374263154987	27.50440271753814
4	12.371751961017667	15.928394143265448	38.27364601895155	33.42620787676533
5	13.007195758226157	37.95240264762732	35.08617049133679	13.954231102809736
6	31.874852895656762	37.31069421141098	17.475314850594962	13.339138042337293
7	28.446994522285472	31.939699732244346	21.619414630749972	17.993891114720203
8	25.543353018926396	36.682596015985105	19.657427543155496	18.116623421933006
9	25.150205660098912	17.089767700580087	20.500433267365697	37.259593371955305
10-11	24.273829049638167	26.88869720887368	28.23801057586811	20.599463165620048
12-13	24.75927905844638	24.099546739111723	28.395563919774887	22.745610282667013
14-15	22.01388988741427	26.221684237770294	25.227941271067728	26.536484603747713
16-17	21.926499921390594	29.5870102071145	25.78861504983231	22.697874821662595
18-19	21.87140831440628	28.507703411787965	27.741798777632624	21.879089496173126
20-21	22.946267606846227	27.836274658003724	27.27524695605485	21.942210779095202
22-23	22.691653443555463	27.588288034835983	27.32852413808239	22.391534383526164
24-25	22.461857530056314	27.591154647609024	27.400201075454756	22.546786746879906
26-27	22.26547371993803	27.592584639502288	27.46028235460607	22.681659285953614
28-29	22.28541450718878	27.664933661028485	27.522968583605113	22.52668324817762
30-31	22.4704752081048	28.128742509305543	27.111744871591608	22.289037410998045
32-33	22.1736081970234	27.793093949515157	27.610958474928044	22.422339378533405
34-35	22.27661979558343	27.481665413302686	27.673841584252074	22.56787320686181
36-37	22.617452419612828	27.90130967968098	27.131911412690513	22.34932648801568
38-39	22.426171301461203	27.99812177830968	27.34363870642589	22.232068213803228
40-41	22.178437543454386	27.704993553379246	27.616509781552733	22.50005912161363
42-43	22.13339402786954	27.719277931784486	27.543437838426904	22.603890201919068
44-45	22.2640081507472	27.862387082919852	27.52176985491178	22.351834911421168
46-47	22.23308687926791	28.038736622864736	27.36126386907214	22.36691262879522
48-49	22.13671070402633	27.789704483848617	27.54843939271811	22.52514541940694
50-51	22.153142042330355	27.706990460930765	27.5529697055933	22.586897791145585
52-53	22.20594343041006	27.610357247263238	27.605399815893865	22.578299506432835
54-55	22.27719550001211	28.09080884133789	27.349539980382183	22.28245567826782
56-57	22.081046256194263	27.865558652294265	27.4697689555413	22.583626135970164
58-59	22.008189695786974	27.396220146227808	27.82827749604649	22.76731266193873
60-61	22.394357643044145	27.89068718593369	27.31819464103234	22.396760529989827
62-63	22.119494368310416	27.844159333392614	27.425543065917164	22.6108032323798
64-65	22.13120290615998	27.50856380638844	27.641270877382183	22.71896241006939
66-67	22.00963682085189	27.58612338664986	27.47509958180655	22.9291402106917
68-69	22.3471661058445	27.744170492460828	27.424572808654794	22.484090593039877
70-71	22.348181630532117	27.498539866884293	27.507545676577532	22.645732826006054
72-73	22.038147557570976	27.617153119227105	27.69075103169516	22.65394829150676
74-75	22.108500215968174	27.662524328346542	27.56243920869564	22.66653624698964
76-77	22.103486904502663	27.62833697126053	27.436624482855894	22.831551641380912
78-79	22.185219464905064	27.719576531380618	27.494753563149843	22.600450440564472
80-81	22.09991319529847	27.534667012775504	27.58628061909083	22.7791391728352
82-83	22.000385585314913	27.54653274987224	27.765869872334935	22.687211792477907
84-85	22.04109417468638	27.537338819014522	27.5709211471799	22.8506458591192
86-87	22.205875536257384	27.573983416816844	27.585505560049	22.63463548687677
88-89	21.888019990835517	27.47652026573221	27.675550767724218	22.959908975708064
90-91	21.921434407876728	27.389212011391383	27.679520915578852	23.009832665153038
92-93	22.114597484975178	27.58059258538944	27.517233589991815	22.787576339643568
94-95	21.925391573160187	27.287224281225107	27.739422936850726	23.047961208763972
96-97	21.94659734339406	27.291044043371564	27.79002555352894	22.972333059705434
98-99	21.91935825016393	27.37282052776137	27.697728895428845	23.010092326645854
100	22.021176689598494	27.20471329665149	27.69243401013884	23.081676003611175
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2318.0
1	1364.0
2	373.0
3	304.5
4	240.5
5	188.5
6	173.5
7	181.0
8	183.5
9	188.5
10	180.0
11	190.0
12	224.0
13	244.5
14	279.0
15	320.5
16	392.5
17	542.5
18	750.0
19	1018.0
20	1488.0
21	2212.0
22	3255.0
23	4985.5
24	7266.5
25	9905.5
26	13718.5
27	18629.0
28	24700.0
29	31756.0
30	38633.0
31	45344.0
32	51871.5
33	58676.0
34	65248.5
35	70626.5
36	75860.0
37	81207.0
38	85186.5
39	87398.0
40	90051.0
41	92307.0
42	92425.0
43	92658.5
44	92050.5
45	91268.5
46	91799.0
47	90735.5
48	89230.5
49	88097.0
50	86096.5
51	83725.0
52	78996.0
53	74798.0
54	72530.5
55	69280.0
56	64729.5
57	58985.5
58	53507.0
59	49844.0
60	45981.5
61	39978.5
62	34371.0
63	29722.0
64	24380.0
65	19274.5
66	15486.0
67	12033.5
68	9077.5
69	6782.5
70	4882.0
71	3558.0
72	2771.0
73	2034.0
74	1480.5
75	1117.0
76	737.0
77	477.5
78	341.0
79	232.5
80	146.5
81	96.0
82	66.5
83	56.0
84	43.0
85	34.0
86	28.0
87	18.0
88	11.0
89	12.0
90	11.5
91	6.5
92	4.0
93	7.0
94	5.5
95	3.0
96	2.0
97	3.0
98	3.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07818954158167461
2	0.08042028769062955
3	0.08200827644615678
4	0.08401216701860782
5	0.08268884305566847
6	0.0821595134704927
7	0.08117647281230919
8	0.08106304504405723
9	0.08110085430014122
10-11	0.08093071264776329
12-13	0.08002329050174772
14-15	0.07822735083775859
16-17	0.07718759629544909
18-19	0.07684731299069325
20-21	0.07662045745418936
22-23	0.07828406472188457
24-25	0.07811392306950665
26-27	0.07726321480761705
28-29	0.07378476324789071
30-31	0.07849201563034647
32-33	0.07720650092349109
34-35	0.07438971134523444
36-37	0.071100306065928
38-39	0.0729529596140431
40-41	0.07552398902775388
42-43	0.0798909581054538
44-45	0.08138442372077108
46-47	0.08467382900007751
48-49	0.08794432965134194
50-51	0.08900298882169344
52-53	0.08913532121798737
54-55	0.08919203510211335
56-57	0.0865831964323186
58-59	0.08567577428630303
60-61	0.08319926801280222
62-63	0.08393654850643986
64-65	0.08289679396413036
66-67	0.08236746437895462
68-69	0.08157347000119099
70-71	0.08002329050174772
72-73	0.08004219512978973
74-75	0.07956957942873995
76-77	0.0792292961239841
78-79	0.07973972108111786
80-81	0.0809496172758053
82-83	0.08013671826999968
84-85	0.0792482007520261
86-87	0.08006109975783171
88-89	0.07728211943565905
90-91	0.07488123167432618
92-93	0.07480561316215824
94-95	0.07524041960712402
96-97	0.07454094836957036
98-99	0.07395490490026864
100	0.07369024010768076
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2644855.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.55218350372519
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.83009264438762	54.60249250936779
2	12.032838733742809	16.25689060425053
3	3.2556867668538194	6.597862497154769
4	1.387516957317934	3.74919200461086
5	0.6759463645843833	2.283082642954009
6	0.41200872508452446	1.6699253401227392
7	0.2582842370265757	1.2213364923017225
8	0.20115166266513612	1.0870587222747758
9	0.15014270497492335	0.9128220802390525
>10	0.7509937437098264	8.919994557513382
>50	0.035995173171137686	1.6160436405217367
>100	0.009174390704957658	0.9685962501333374
>500	1.1193051756567456E-4	0.04687913534334608
>1k	5.596525876424536E-5	0.06782352321197126
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.780925608398192E-5	0.0
3	0.0	0.0	0.0	3.780925608398192E-5	0.0
4	0.0	0.0	0.0	7.561851216796384E-5	0.0
5	0.0	0.0	0.0	7.561851216796384E-5	0.0
6	0.0	0.0	0.0	7.561851216796384E-5	0.0
7	0.0	0.0	0.0	7.561851216796384E-5	0.0
8	0.0	0.0	0.0	7.561851216796384E-5	0.0
9	0.0	0.0	0.0	1.1342776825194575E-4	0.0
10-11	0.0	0.0	0.0	1.1342776825194575E-4	0.0
12-13	0.0	0.0	0.0	1.1342776825194575E-4	0.0
14-15	0.0	0.0	0.0	1.1342776825194575E-4	0.0
16-17	0.0	0.0	0.0	1.1342776825194575E-4	0.0
18-19	0.0	0.0	0.0	1.5123702433592767E-4	0.0
20-21	0.0	0.0	0.0	1.5123702433592767E-4	0.0
22-23	0.0	0.0	0.0	2.835694206298644E-4	0.0
24-25	0.0	0.0	0.0	4.348064449657921E-4	0.0
26-27	0.0	0.0	0.0	5.293295851757469E-4	0.0
28-29	0.0	0.0	0.0	0.001361133219023349	0.0
30-31	0.0	0.0	0.0	0.004915203290917649	0.0
32-33	0.0	0.0	0.0	0.013857092354779374	0.0
34-35	0.0	0.0	0.0	0.023782022076824626	0.0
36-37	0.0	0.0	0.0	0.043197075075949345	0.0
38-39	0.0	0.0	0.0	0.08198937181811479	0.0
40-41	0.0	0.0	0.0	0.13055536125798956	0.0
42-43	0.0	0.0	0.0	0.18061481631318163	0.0
44-45	0.0	0.0	0.0	0.23575961631166925	0.0
46-47	0.0	0.0	0.0	0.3064818298167574	0.0
48-49	0.0	0.0	0.0	0.3908153755120791	0.0
50-51	0.0	0.0	0.0	0.48492261390511016	0.0
52-53	0.0	0.0	0.0	0.5764588228844303	0.0
54-55	0.0	0.0	0.0	0.6769369209276123	0.0
56-57	0.0	0.0	0.0	0.7912721113255736	0.0
58-59	0.0	0.0	0.0	0.9269317221549007	0.0
60-61	0.0	0.0	0.0	1.072875450639071	0.0
62-63	0.0	0.0	0.0	1.2225433908475134	0.0
64-65	0.0	0.0	0.0	1.382684494991219	0.0
66-67	0.0	0.0	0.0	1.5634316437006945	0.0
68-69	0.0	0.0	0.0	1.7694164708462279	0.0
70-71	0.0	0.0	0.0	1.9931149344671066	0.0
72-73	0.0	0.0	0.0	2.2274377990475847	0.0
74-75	0.0	0.0	0.0	2.4639157912248497	0.0
76-77	0.0	0.0	0.0	2.719033746651518	0.0
78-79	0.0	0.0	0.0	3.0097302120532126	0.0
80-81	0.0	0.0	0.0	3.3251161216777483	0.0
82-83	0.0	0.0	0.0	3.6521661868041915	0.0
84-85	0.0	0.0	0.0	3.9740741931032133	0.0
86-87	0.0	0.0	0.0	4.309517913080301	0.0
88	0.0	0.0	0.0	4.583200213244204	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3315	0.0	35.588573	1
TCAACGC	3270	0.0	33.634773	4
ATCAACG	3435	0.0	32.01913	3
CAACGCA	3450	0.0	31.878107	5
GGTATCA	2175	0.0	30.68665	1
AACGCAG	3610	0.0	30.464073	6
GTACATG	8325	0.0	28.399073	1
TATCAAC	4085	0.0	27.499592	2
TACATGG	8320	0.0	27.22978	2
ACATGGG	8705	0.0	24.94558	3
ACGCAGA	4535	0.0	24.14671	7
GAGTACA	6040	0.0	22.333942	1
CGCAGAG	4895	0.0	21.986803	8
CATGGGG	5790	0.0	20.375843	4
AGTACAT	6170	0.0	19.806538	2
TACGACC	265	2.0962034E-8	19.510447	4
GTACGAC	245	1.9642539E-7	19.18467	3
TACCTGG	1755	0.0	18.479591	2
GCAGAGT	5730	0.0	18.372341	9
GTATAGG	1100	0.0	17.946386	1
>>END_MODULE